Polarimetric radar observations and interpretation of co-cross-polar correlation coefficients

Includes bibliographical references (page 354).Preliminary analysis of all components of the polarimetric radar covariance matrix for precipitation measured with the NCAR S-band dual-polarization Doppler radar (S-Pol) and the Colorado State University-University of Chicago-Illinois State Water Survey (CSU-CHILL) radars is presented. Radar reflectivity at horizontal polarization Zh, differential reflectivity ZDR, linear depolarization ratio LDR, specific differential phase KDP, crosscorrelation coefficient | ρhv | , and two co-cross-polar correlation coefficients, ρxh and ρxv, have been measured and examined for two rain events: the 14 August 1998 case in Florida and the 8 August 1998 case in Colorado. Examination of the coefficients ρxh and ρxv is the major focus of the study. It is shown that hydrometeors with different types of orientation can be better delineated if the coefficients ρxv and ρxv are used. Rough estimates of the raindrop mean canting angles and the rms width of the canting angle distribution are obtained from the co-cross-polar correlation coefficients in combination with other polarimetric variables. Analysis of the two cases indicates that the raindrop net canting angles averaged over the propagation paths near the ground in typical convective cells do not exceed 2.5°. Nonetheless, the mean canting angles in individual radar resolution volumes in rain can be noticeably higher. Although the net canting angle for individual convective cells can deviate by a few degrees from zero, the average over a long propagation path along several cells is close to zero, likely because canting angles in different cells vary in sign. The rms width of the canting angle distribution in rain is estimated to vary mainly between 5° and 15° with the median value slightly below 10°

Analysis of meniscal degeneration and meniscal gene expression

<p>Abstract</p> <p>Background</p> <p>Menisci play a vital role in load transmission, shock absorption and joint stability. There is increasing evidence suggesting that OA menisci may not merely be bystanders in the disease process of OA. This study sought: 1) to determine the prevalence of meniscal degeneration in OA patients, and 2) to examine gene expression in OA meniscal cells compared to normal meniscal cells.</p> <p>Methods</p> <p>Studies were approved by our human subjects Institutional Review Board. Menisci and articular cartilage were collected during joint replacement surgery for OA patients and lower limb amputation surgery for osteosarcoma patients (normal control specimens), and graded. Meniscal cells were prepared from these meniscal tissues and expanded in monolayer culture. Differential gene expression in OA meniscal cells and normal meniscal cells was examined using Affymetrix microarray and real time RT-PCR.</p> <p>Results</p> <p>The grades of meniscal degeneration correlated with the grades of articular cartilage degeneration (r = 0.672; P < 0.0001). Many of the genes classified in the biological processes of immune response, inflammatory response, biomineral formation and cell proliferation, including major histocompatibility complex, class II, DP alpha 1 (<it>HLA-DPA1</it>), integrin, beta 2 (<it>ITGB2</it>), ectonucleotide pyrophosphatase/phosphodiesterase 1 (<it>ENPP1</it>), ankylosis, progressive homolog (<it>ANKH</it>) and fibroblast growth factor 7 (<it>FGF7</it>), were expressed at significantly higher levels in OA meniscal cells compared to normal meniscal cells. Importantly, many of the genes that have been shown to be differentially expressed in other OA cell types/tissues, including ADAM metallopeptidase with thrombospondin type 1 motif 5 (<it>ADAMTS5</it>) and prostaglandin E synthase (<it>PTGES</it>), were found to be expressed at significantly higher levels in OA meniscal cells. This consistency suggests that many of the genes detected in our study are disease-specific.</p> <p>Conclusion</p> <p>Our findings suggest that OA is a whole joint disease. Meniscal cells may play an active role in the development of OA. Investigation of the gene expression profiles of OA meniscal cells may reveal new therapeutic targets for OA therapy and also may uncover novel disease markers for early diagnosis of OA.</p

Fibronectin Matrix Assembly Suppresses Dispersal of Glioblastoma Cells

Glioblastoma (GBM), the most aggressive and most common form of primary brain tumor, has a median survival of 12–15 months. Surgical excision, radiation and chemotherapy are rarely curative since tumor cells broadly disperse within the brain. Preventing dispersal could be of therapeutic benefit. Previous studies have reported that increased cell-cell cohesion can markedly reduce invasion by discouraging cell detachment from the tumor mass. We have previously reported that α5β1 integrin-fibronectin interaction is a powerful mediator of indirect cell-cell cohesion and that the process of fibronectin matrix assembly (FNMA) is crucial to establishing strong bonds between cells in 3D tumor-like spheroids. Here, we explore a potential role for FNMA in preventing dispersal of GBM cells from a tumor-like mass. Using a series of GBM-derived cell lines we developed an in vitro assay to measure the dispersal velocity of aggregates on a solid substrate. Despite their similar pathologic grade, aggregates from these lines spread at markedly different rates. Spreading velocity is inversely proportional to capacity for FNMA and restoring FNMA in GBM cells markedly reduces spreading velocity by keeping cells more connected. Blocking FNMA using the 70 KDa fibronectin fragment in FNMA-restored cells rescues spreading velocity, establishing a functional role for FNMA in mediating dispersal. Collectively, the data support a functional causation between restoration of FNMA and decreased dispersal velocity. This is a first demonstration that FNMA can play a suppressive role in GBM dispersal

The Changing Landscape for Stroke\ua0Prevention in AF: Findings From the GLORIA-AF Registry Phase 2

Background GLORIA-AF (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients with Atrial Fibrillation) is a prospective, global registry program describing antithrombotic treatment patterns in patients with newly diagnosed nonvalvular atrial fibrillation at risk of stroke. Phase 2 began when dabigatran, the first non\u2013vitamin K antagonist oral anticoagulant (NOAC), became available. Objectives This study sought to describe phase 2 baseline data and compare these with the pre-NOAC era collected during phase&nbsp;1. Methods During phase 2, 15,641 consenting patients were enrolled (November 2011 to December 2014); 15,092 were eligible. This pre-specified cross-sectional analysis describes eligible patients\u2019 baseline characteristics. Atrial fibrillation&nbsp;disease characteristics, medical outcomes, and concomitant diseases and medications were collected. Data were analyzed using descriptive statistics. Results Of the total patients, 45.5% were female; median age was 71 (interquartile range: 64, 78) years. Patients were from Europe (47.1%), North America (22.5%), Asia (20.3%), Latin America (6.0%), and the Middle East/Africa (4.0%). Most had high stroke risk (CHA2DS2-VASc [Congestive heart failure, Hypertension, Age&nbsp; 6575 years, Diabetes mellitus, previous Stroke, Vascular disease, Age 65 to 74 years, Sex category] score&nbsp; 652; 86.1%); 13.9% had moderate risk (CHA2DS2-VASc&nbsp;= 1). Overall, 79.9% received oral anticoagulants, of whom 47.6% received NOAC and 32.3% vitamin K antagonists (VKA); 12.1% received antiplatelet agents; 7.8% received no antithrombotic treatment. For comparison, the proportion of phase 1 patients (of N&nbsp;= 1,063 all eligible) prescribed VKA was 32.8%, acetylsalicylic acid 41.7%, and no therapy 20.2%. In Europe in phase 2, treatment with NOAC was more common than VKA (52.3% and 37.8%, respectively); 6.0% of patients received antiplatelet treatment; and 3.8% received no antithrombotic treatment. In North America, 52.1%, 26.2%, and 14.0% of patients received NOAC, VKA, and antiplatelet drugs, respectively; 7.5% received no antithrombotic treatment. NOAC use was less common in Asia (27.7%), where 27.5% of patients received VKA, 25.0% antiplatelet drugs, and 19.8% no antithrombotic treatment. Conclusions The baseline data from GLORIA-AF phase 2 demonstrate that in newly diagnosed nonvalvular atrial fibrillation patients, NOAC have been highly adopted into practice, becoming more frequently prescribed than VKA in&nbsp;Europe and North America. Worldwide, however, a large proportion of patients remain undertreated, particularly in&nbsp;Asia&nbsp;and North America. (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients With Atrial Fibrillation [GLORIA-AF]; NCT01468701

Relationships between thunderstorm mesoscale circulation and tornadogenesis.

The data show that vertical vorticity production in tornadic storms begins at the very roots of the updraft as horizontal vorticity associated with low level environmental air is tilted toward the vertical. The vertical vorticity produced is amplified by the convergence mechanism within strong updraft to create the low level mesocyclone.A sudden intensification of mesocyclone rotation, particularly at low levels, heralds the tornadic stage and transforms the mesocyclone. Updrafts and rainy downdrafts are strong at this critical stage and interact to locally increase convergence. Low level vorticity amplification by the convergence mechanism surges, exceeding maximum twisting term generation by a factor of 2 or more. Tornadoes feed upon low level air which passes through the region of strong convergence term amplification and are most likely triggered by that vorticity production.A methodology for obtaining pressure and potential temperature deviations in thunderstorm flows reconstructed from Doppler radar observations is described. Results are combined with detailed vorticity analyses to study properties of severe thunderstorm circulations (mesocyclones) known to have spawned tornadoes.The dependence on vorticity causes the low level pressure deficit associated with the mesocyclone to deepen during intensification. Upward perturbation pressure gradients in vicinity of the mesocyclone are reduced and can be reversed by the build-up of low level vorticity. The sudden formation of concentrated rear downdrafts commonly observed in tornadic thunderstorms results from the vertical pressure gradient reversal. Mesocyclone intensification may also precipitate storm decline. The reduction in the vertical perturbation pressure gradient decreases the storm's ability to lift negatively buoyant air at the base of updrafts. Further, downdraft formation results in a flux of air parcels into the mesocyclone from higher levels on the storm's rear. This air is potentially cold and when mixed with updraft air reduces buoyancy in middle storm levels. In final stages, updrafts weaken and downdrafts fill the mesocyclone