13 research outputs found
The Ursinus Weekly, February 10, 1905
Society notes β’ Only an amateur β’ Alumni notes β’ Dr. Miller to preach β’ Sophomore reception β’ St. Valentine\u27s treat β’ College world β’ College notes β’ Noticehttps://digitalcommons.ursinus.edu/weekly/3002/thumbnail.jp
The Ursinus Weekly, May 11, 1906
Baseball β’ Schaff prize debate β’ Lecture recital β’ A reply β’ Personals β’ The Rutgers gamehttps://digitalcommons.ursinus.edu/weekly/2979/thumbnail.jp
The Ursinus Weekly, May 25, 1906
Exercises of Commencement Week β’ Baseball β’ The freshman banquet β’ Society notes β’ Report of Field House Committee β’ Announcementhttps://digitalcommons.ursinus.edu/weekly/2981/thumbnail.jp
The Ursinus Weekly, May 4, 1906
Baseball β’ Memorial tablet unveiled β’ Schaff prize debate β’ Dr. Shaw on a leave of absence β’ Society notes β’ Alumni β’ The business manager\u27s song β’ Personalshttps://digitalcommons.ursinus.edu/weekly/2978/thumbnail.jp
The Ursinus Weekly, June 1, 1906
Exercises of Commencement Week β’ Song recital β’ Junior oratorical contest β’ Commencement orations β’ Alumni notes β’ Baseball β’ Provisional football schedule β’ Society notes β’ Glee concert β’ College noteshttps://digitalcommons.ursinus.edu/weekly/2982/thumbnail.jp
The Ursinus Weekly, April 20, 1906
Baseball β’ Ursinus Union β’ Fire at Olevian Hall β’ Society notes β’ Notes on the baseball triphttps://digitalcommons.ursinus.edu/weekly/2976/thumbnail.jp
The Ursinus Weekly, April 20, 1906
Baseball β’ Ursinus Union β’ Fire at Olevian Hall β’ Society notes β’ Notes on the baseball triphttps://digitalcommons.ursinus.edu/weekly/2976/thumbnail.jp
The Ursinus Weekly, May 18, 1906
Baseball β’ Ursinus Union β’ Notice β’ Society notes β’ The Union as viewed by a charter member β’ College notes β’ Class game β’ Heard on the campushttps://digitalcommons.ursinus.edu/weekly/2980/thumbnail.jp
The Ursinus Weekly, June 8, 1906
A dream of Heaven β’ The Baccalaureate sermon β’ Song recital β’ Class Day β’ The junior oratorical contest β’ Commencement β’ The Charmidean banquet β’ Society notes β’ Baseball β’ Alumni Day β’ Commencement game β’ Literary Supplement: A twentieth century renaissance; The college man in public life; Formation of the Schuylkill Valley; Does prevalence of natural science tend to check poetic spirit?; Janice Meredith and the modern girlhttps://digitalcommons.ursinus.edu/weekly/2983/thumbnail.jp
Respiratory Syncytial Virus NS1 Protein Colocalizes with Mitochondrial Antiviral Signaling Protein MAVS following Infection
Respiratory syncytial virus (RSV) nonstructural protein 1(NS1) attenuates type-I interferon (IFN) production during RSV infection; however the precise role of RSV NS1 protein in orchestrating the early host-virus interaction during infection is poorly understood. Since NS1 constitutes the first RSV gene transcribed and the production of IFN depends upon RLR (RIG-I-like receptor) signaling, we reasoned that NS1 may interfere with this signaling. Herein, we report that NS1 is localized to mitochondria and binds to mitochondrial antiviral signaling protein (MAVS). Live-cell imaging of rgRSV-infected A549 human epithelial cells showed that RSV replication and transcription occurs in proximity to mitochondria. NS1 localization to mitochondria was directly visualized by confocal microscopy using a cell-permeable chemical probe for His6-NS1. Further, NS1 colocalization with MAVS in A549 cells infected with RSV was shown by confocal laser microscopy and immuno-electron microscopy. NS1 protein is present in the mitochondrial fraction and co-immunoprecipitates with MAVS in total cell lysatesof A549 cells transfected with the plasmid pNS1-Flag. By immunoprecipitation with anti-RIG-I antibody, RSV NS1 was shown to associate with MAVS at an early stage of RSV infection, and to disrupt MAVS interaction with RIG-I (retinoic acid inducible gene) and the downstream IFN antiviral and inflammatory response. Together, these results demonstrate that NS1 binds to MAVS and that this binding inhibits the MAVS-RIG-I interaction required for IFN production