The Ursinus Weekly, February 10, 1905

Society notes • Only an amateur • Alumni notes • Dr. Miller to preach • Sophomore reception • St. Valentine\u27s treat • College world • College notes • Noticehttps://digitalcommons.ursinus.edu/weekly/3002/thumbnail.jp

The Ursinus Weekly, May 11, 1906

Baseball • Schaff prize debate • Lecture recital • A reply • Personals • The Rutgers gamehttps://digitalcommons.ursinus.edu/weekly/2979/thumbnail.jp

The Ursinus Weekly, May 25, 1906

Exercises of Commencement Week • Baseball • The freshman banquet • Society notes • Report of Field House Committee • Announcementhttps://digitalcommons.ursinus.edu/weekly/2981/thumbnail.jp

The Ursinus Weekly, May 4, 1906

Baseball • Memorial tablet unveiled • Schaff prize debate • Dr. Shaw on a leave of absence • Society notes • Alumni • The business manager\u27s song • Personalshttps://digitalcommons.ursinus.edu/weekly/2978/thumbnail.jp

The Ursinus Weekly, June 1, 1906

Exercises of Commencement Week • Song recital • Junior oratorical contest • Commencement orations • Alumni notes • Baseball • Provisional football schedule • Society notes • Glee concert • College noteshttps://digitalcommons.ursinus.edu/weekly/2982/thumbnail.jp

The Ursinus Weekly, April 20, 1906

Baseball • Ursinus Union • Fire at Olevian Hall • Society notes • Notes on the baseball triphttps://digitalcommons.ursinus.edu/weekly/2976/thumbnail.jp

The Ursinus Weekly, April 20, 1906

Baseball • Ursinus Union • Fire at Olevian Hall • Society notes • Notes on the baseball triphttps://digitalcommons.ursinus.edu/weekly/2976/thumbnail.jp

The Ursinus Weekly, May 18, 1906

Baseball • Ursinus Union • Notice • Society notes • The Union as viewed by a charter member • College notes • Class game • Heard on the campushttps://digitalcommons.ursinus.edu/weekly/2980/thumbnail.jp

The Ursinus Weekly, June 8, 1906

A dream of Heaven • The Baccalaureate sermon • Song recital • Class Day • The junior oratorical contest • Commencement • The Charmidean banquet • Society notes • Baseball • Alumni Day • Commencement game • Literary Supplement: A twentieth century renaissance; The college man in public life; Formation of the Schuylkill Valley; Does prevalence of natural science tend to check poetic spirit?; Janice Meredith and the modern girlhttps://digitalcommons.ursinus.edu/weekly/2983/thumbnail.jp

Respiratory Syncytial Virus NS1 Protein Colocalizes with Mitochondrial Antiviral Signaling Protein MAVS following Infection

Respiratory syncytial virus (RSV) nonstructural protein 1(NS1) attenuates type-I interferon (IFN) production during RSV infection; however the precise role of RSV NS1 protein in orchestrating the early host-virus interaction during infection is poorly understood. Since NS1 constitutes the first RSV gene transcribed and the production of IFN depends upon RLR (RIG-I-like receptor) signaling, we reasoned that NS1 may interfere with this signaling. Herein, we report that NS1 is localized to mitochondria and binds to mitochondrial antiviral signaling protein (MAVS). Live-cell imaging of rgRSV-infected A549 human epithelial cells showed that RSV replication and transcription occurs in proximity to mitochondria. NS1 localization to mitochondria was directly visualized by confocal microscopy using a cell-permeable chemical probe for His6-NS1. Further, NS1 colocalization with MAVS in A549 cells infected with RSV was shown by confocal laser microscopy and immuno-electron microscopy. NS1 protein is present in the mitochondrial fraction and co-immunoprecipitates with MAVS in total cell lysatesof A549 cells transfected with the plasmid pNS1-Flag. By immunoprecipitation with anti-RIG-I antibody, RSV NS1 was shown to associate with MAVS at an early stage of RSV infection, and to disrupt MAVS interaction with RIG-I (retinoic acid inducible gene) and the downstream IFN antiviral and inflammatory response. Together, these results demonstrate that NS1 binds to MAVS and that this binding inhibits the MAVS-RIG-I interaction required for IFN production