The Different Nature in Seyfert 2 Galaxies With and Without Hidden Broad-Line Regions

We compile a large sample of 120 Seyfert 2 galaxies (Sy2s) which contains 49 hidden broad-line region (HBLR) Sy2s and 71 non-HBLR Sy2s. From the difference in the power sources between two groups, we test if HBLR Sy2s are dominated by active galactic nuclei (AGNs), and if non-HBLR Sy2s are dominated by starbursts. We show that: (1) HBLR Sy2s have larger accretion rates than non-HBLR Sy2s; (2) HBLR Sy2s have larger \Nev $\lambda 14.32$/\Neii $\lambda 12.81$ and \oiv $\lambda 25.89$/\Neii $\lambda 12.81$ line ratios than non-HBLR Sy2s; (3) HBLR Sy2s have smaller $IRAS$ $f_{60}/f_{25}$ flux ratio which shows the relative strength of the host galaxy and nuclear emission than non-HBLR Sy2s. So we suggest that HBLR Sy2s and non-HBLR Sy2s are AGN-dominated and starburst-dominated, respectively. In addition, non-HBLR Sy2s can be classified into the luminous ($L_{\rm [O III]}>10^{41} \rm ergs s^{-1}$) and less luminous ($L_{\rm [O III]}<10^{41} \rm ergs s^{-1}$) samples, when considering only their obscuration. We suggest that: (1) the invisibility of polarized broad lines (PBLs) in the luminous non-HBLR Sy2s depends on the obscuration; (2) the invisibility of PBLs in the less luminous non-HBLR Sy2s depends on the very low Eddington ratio rather than the obscuration.Comment: Accepted by ApJ, 11 pages, 4 figure

Determinants of Survival of HIV Patients Receiving Dolutegravir: A Prospective Cohort Study in Conflict-Affected Bunia, Democratic Republic of Congo.

This study aims to determine the factors influencing HIV-related mortality in settings experiencing continuous armed conflict atrocities. In such settings, people living with HIV (PLHIV), and the partners of those affected may encounter specific difficulties regarding adherence to antiretroviral therapy (ART), and retention in HIV prevention, treatment, and care programs. Between July 2019 and July 2021, we conducted an observational prospective cohort study of 468 PLHIV patients treated with Dolutegravir at all the ART facilities in Bunia. The probability of death being the primary outcome, as a function of time of inclusion in the cohort, was determined using Kaplan-Meier plots. We used the log-rank test to compare survival curves and Cox proportional hazard modeling to determine mortality predictors from the baseline to 31 July 2021 (endpoint). The total number of person-months (p-m) was 3435, with a death rate of 6.70 per 1000 p-m. Compared with the 35-year-old reference group, older patients had a higher mortality risk. ART-naïve participants at the time of enrollment had a higher mortality risk than those already using ART. Patients with a high baseline viral load (≥1000 copies/mL) had a higher mortality risk compared with the reference group (adjusted hazard ratio = 6.04; 95% CI: 1.78-20.43). One-fourth of deaths in the cohort were direct victims of armed conflict, with an estimated excess death of 35.6%. Improving baseline viral load monitoring, starting ART early in individuals with high baseline viral loads, the proper tailoring of ART regimens and optimizing long-term ART, and care to manage non-AIDS-related chronic complications are recommended actions to reduce mortality. Not least, fostering women's inclusion, justice, peace, and security in conflict zones is critical in preventing premature deaths in the general population as well as among PLHIV

Prospectives

Tiré de: Prospectives, vol. 10, no 3, juin 1974Titre de l'écran-titre (visionné le 24 janv. 2013

Molecular insights of nickel binding to therapeutic antibodies as a possible new antibody superantigen

The binding of nickel by immune proteins can manifest as Type IV contact dermatitis (Ni-specific T cells mediated) and less frequently as Type I hypersensitivity with both mechanisms remaining unknown to date. Since there are reports of patients co-manifesting the two hypersensitivities, a common mechanism may underlie both the TCR and IgE nickel binding. Focusing on Trastuzumab and Pertuzumab IgE variants as serendipitous investigation models, we found Ni-NTA interactions independent of Her2 binding to be due to glutamine stretches. These stretches are both Ni-inducible and in fixed pockets at the antibody complementarity-determining regions (CDRs) and framework regions (FWRs) of both the antibody heavy and light chains with influence from the heavy chain constant region. Comparisons with TCRs structures revealed similar interactions, demonstrating the possible underlying mechanism in selecting for Ni-binding IgEs and TCRs respectively. With the elucidation of the interaction, future therapeutic antibodies could also be sagaciously engineered to utilize such nickel binding for biotechnological purposes

Towards 100 % recycling of reclaimed asphalt in road surface courses: binder design methodology and case studies

Reclaimed Asphalt (RA) has shown great potential to be reused in new asphalt mixtures, however its incorporation in top asphalt pavement layers is still very limited (10-30%). In fact, despite the advantages that its use implies, RA content in road pavement surface courses is still restricted in most countries due to mainly legislation limitations, but also some technical issues. This paper aims at being a step further to improve the latter by providing a methodology that allows producing fundamental inputs for confidently performing mix design of asphalt mixtures incorporating up to 100% RA. The methodology consists in an advanced preliminary binder’s blend design that can be used with any type of RA and also in presence of rejuvenators. This procedure includes in the production of blending charts and laws that considers the uncertainties on accounting the extent of final binder content, Degree of Blending and Replaced Virgin Binder. The description of the methodology is accompanied with results of two extreme case studies consisting in the preliminary design of binders for asphalt mixtures with high content of two types of RA corresponding to extreme cases: the short-term aged RA (STA-RA), having a very soft residual binder (Pen> 20dmm) and the long-term aged RA, having a much harder residual binder (Pen <10dmm). As a result, the proposed methodology allowed assessing the feasibility of using up to 90% of RA and determining whether the use of rejuvenating agents was needed

Variable-heavy (VH) families influencing IgA1&2 engagement to the antigen, FcαRI and superantigen proteins G, A, and L

Interest in IgA as an alternative antibody format has increased over the years with much remaining to be investigated in relation to interactions with immune cells. Considering the recent whole antibody investigations showing significant distal effects between the variable (V) and constant (C)- regions that can be mitigated by the hinge regions of both human IgA subtypes A1 and A2, we performed an in-depth mechanistic investigation using a panel of 28 IgA1s and A2s of both Trastuzumab and Pertuzumab models. FcαRI binding were found to be mitigated by the differing glycosylation patterns in IgA1 and 2 with contributions from the CDRs. On their interactions with antigen-Her2 and superantigens PpL, SpG and SpA, PpL was found to sterically hinder Her2 antigen binding with unexpected findings of IgAs binding SpG at the CH2-3 region alongside SpA interacting with IgAs at the CH1. Although the VH3 framework (FWR) is commonly used in CDR grafting, we found the VH1 framework (FWR) to be a possible alternative when grafting IgA1 and 2 owing to its stronger binding to antigen Her2 and weaker interactions to superantigen Protein L and A. These findings lay the foundation to understanding the interactions between IgAs and microbial superantigens, and also guide the engineering of IgAs for future antibody applications and targeting of superantigen-producing microbes

Textile-reinforced mortar (TRM) versus fibre-reinforced polymers (FRP) in flexural strengthening of RC beams

The aim of this paper is to compare the flexural performance of reinforced concrete (RC) beams strengthened with textile-reinforced mortar (TRM) and fibre-reinforced polymers (FRP). The investigated parameters included the strengthening material, namely TRM or FRP; the number of TRM/FRP layers; the textile surface condition (coated and uncoated); the textile fibre material (carbon, coated basalt or glass fibres); and the end-anchorage system of the external reinforcement. Thirteen RC beams were fabricated, strengthened and tested in four-point bending. One beam served as control specimen, seven beams strengthened with TRM, and five with FRP. It was mainly found that: (a) TRM was generally inferior to FRP in enhancing the flexural capacity of RC beams, with the effectiveness ratio between the two systems varying from 0.46 to 0.80, depending on the parameters examined, (b) by tripling the number of TRM layers (from one to three), the TRM versus FRP effectiveness ratio was almost doubled, (c) providing coating to the dry textile enhanced the TRM effectiveness and altered the failure mode; (d) different textile materials, having approximately same axial stiffness, resulted in different flexural capacity increases; and (e) providing end-anchorage had a limited effect on the performance of TRM-retrofitted beams. Finally, a simple formula proposed by fib Model Code 2010 for FRP reinforcement was used to predict the mean debonding stress developed in the TRM reinforcement. It was found that this formula is in a good agreement with the average stress calculated based on the experimental results when failure was similar to FRP-strengthened beams

Interleukin-1β sequesters hypoxia inducible factor 2α to the primary cilium.

BACKGROUND: The primary cilium coordinates signalling in development, health and disease. Previously we have shown that the cilium is essential for the anabolic response to loading and the inflammatory response to interleukin-1β (IL-1β). We have also shown the primary cilium elongates in response to IL-1β exposure. Both anabolic phenotype and inflammatory pathology are proposed to be dependent on hypoxia-inducible factor 2 alpha (HIF-2α). The present study tests the hypothesis that an association exists between the primary cilium and HIFs in inflammatory signalling. RESULTS: Here we show, in articular chondrocytes, that IL-1β-induces primary cilia elongation with alterations to cilia trafficking of arl13b. This elongation is associated with a transient increase in HIF-2α expression and accumulation in the primary cilium. Prolyl hydroxylase inhibition results in primary cilia elongation also associated with accumulation of HIF-2α in the ciliary base and axoneme. This recruitment and the associated cilia elongation is not inhibited by blockade of HIFα transcription activity or rescue of basal HIF-2α expression. Hypomorphic mutation to intraflagellar transport protein IFT88 results in limited ciliogenesis. This is associated with increased HIF-2α expression and inhibited response to prolyl hydroxylase inhibition. CONCLUSIONS: These findings suggest that ciliary sequestration of HIF-2α provides negative regulation of HIF-2α expression and potentially activity. This study indicates, for the first time, that the primary cilium regulates HIF signalling during inflammation

Bond between textile-reinforced mortar (TRM) and concrete substrates: experimental investigation

This paper presents an extended experimental study on the bond behaviour between textile-reinforced mortar (TRM) and concrete substrates. The parameters examined include: (a) the bond length (from 50 mm to 450 mm); (b) the number of TRM layers (from one to four); (c) the concrete surface preparation (grinding versus sandblasting); (d) the concrete compressive strength (15 MPa or 30 MPa); (e) the textile coating; and (f) the anchorage through wrapping with TRM jackets. For this purpose, a total of 80 specimens were fabricated and tested under double-lap direct shear. It is mainly concluded that: (a) after a certain bond length (between 200 mm and 300 mm for any number of layers) the bond strength marginally increases; (b) by increasing the number of layers the bond capacity increases in a non-proportional way, whereas the failure mode is altered; (c) concrete sandblasting is equivalent to grinding in terms of bond capacity and failure mode; (d) concrete compressive strength has a marginal effect on the bond capacity; (e) the use of coated textiles alters the failure mode and significantly increases the bond strength; and (f) anchorage of TRM through wrapping with TRM jackets substantially increases the ultimate load capacity