Pokusna infekcija virusom orf u ovaca i koza.

Typical orf lesions were successfully produced (experimently) on the udder dermis of lactating sheep and goats. The animals were subjected to daily clinical examination and the developing lesions were monitored and recorded. The lesions involved were erythema, papules, pustules and firm irregular scabs on the skin of the udders. One goat developed unilateral moderate udder fibrosis and two of the sheep developed bilateral similar lesions. Examination of milk samples from infected animals by California Mastitis Test (CMT) and Mastitis Yellow Card Test was found positive for mastitis. Staphylococcus aureus, Staphylococcus epidermidis, Proteus mirabilis and Arcanobacterium pyogenes were recovered from the milk of affected animals. All the infected animals were sero-converted to orf infection and produced neutralizing antibodies. The causative orf virus was re-isolated from the scabs of their lesions. The bionomics of the disease was discussed.Pokusna infekcija virusom orf za vrijeme laktacije očitovala se je tipičnim promjenama na koži vimena u ovaca i koza. Životinje su bile dnevno klinički promatrane, a sve uočene promjene zabilježene. Promjene su se očitovale pojavom eritema, papula, pustula i čvrstih nepravilnih krasta na koži vimena. U jedne koze razvila se jednostrana umjerena fibroza vimena, a u dvije su se takve promjene razvile obostrano. Pretraga uzoraka mlijeka kalifornijskim mastitis testom i mjerenjem pH pokazala je da se u zaraženih životinja razvio mastitis. Staphylococcus aureus, Staphylococcus epidermidis, Proteus mirabilis i Arcanobaterium pyogenes izdvojeni su iz vimena zaraženih životinja. U svih zaraženih životinja dokazana su neutralizacijska protutijela za virus orf. Virus je bio ponovo izdvojen iz krasta nastalih nakon pokusne infekcije

Veterinarsko značenje komarčića roda Culicoides (Ceratopogonidae) u nekim podruèjima Saudijske Arabije.

Despite reports of severe outbreaks of arboviral diseases in domestic animals in Saudi Arabia, very little effort has been made to study the potential local vectors of these diseases. Diseases such as bluetongue and African horse sickness were reported in Saudi Arabia. Such diseases are internationally known to be vectored by Culicoides midges. The present study was undertaken at three localities in Saudi Arabia where arboviral activity had previously been reported. Results revealed the presence of four Culicoides species in the three localities. Two of these species were confirmed as vectors of arboviruses of domestic animals in neighbouring countries. The results were discussed in relation to the epidemiology of arboviral diseases in Saudi Arabia.Usprkos pojave teških arbovirusnih zaraza u domaćih životinja u Saudijskoj Arabiji, vrlo malo pozornosti posvećuje se istraživanjima mogućih vektora. U Saudijskoj Arabiji javljaju se bolesti kao što su bolest plavog jezika i konjska kuga. Poznato je da njih prenose komarčići roda Culicoides. Istraživanje je poduzeto na trima područjima u Saudijskoj Arabiji na kojima je već prethodno dokazana aktivnost arbovirusa. Dokazane su četiri vrste komarčića. Potvrđeno je da dvije od tih vrsta služe kao vektori arbovirusa u domaćih životinja. Rezultati su razmatrani u sklopu epizootiologije arbovirusnih bolesti u Saudijskoj Arabiji