Roaring high and low: composition and possible functions of the Iberian stag's vocal repertoire

We provide a detailed description of the rutting vocalisations of free-ranging male Iberian deer (Cervus elaphus hispanicus, Hilzheimer 1909), a geographically isolated and morphologically differentiated subspecies of red deer Cervus elaphus. We combine spectrographic examinations, spectral analyses and automated classifications to identify different call types, and compare the composition of the vocal repertoire with that of other red deer subspecies. Iberian stags give bouts of roars (and more rarely, short series of barks) that are typically composed of two different types of calls. Long Common Roars are mostly given at the beginning or at the end of the bout, and are characterised by a high fundamental frequency (F0) resulting in poorly defined formant frequencies but a relatively high amplitude. In contrast, Short Common Roars are typically given in the middle or at the end of the bout, and are characterised by a lower F0 resulting in relatively well defined vocal tract resonances, but low amplitude. While we did not identify entirely Harsh Roars (as described in the Scottish red deer subspecies (Cervus elaphus scoticus), a small percentage of Long Common Roars contained segments of deterministic chaos. We suggest that the evolution of two clearly distinct types of Common Roars may reflect divergent selection pressures favouring either vocal efficiency in high pitched roars or the communication of body size in low-pitched, high spectral density roars highlighting vocal tract resonances. The clear divergence of the Iberian red deer vocal repertoire from those of other documented European red deer populations reinforces the status of this geographical variant as a distinct subspecies

Emergent complex neural dynamics

A large repertoire of spatiotemporal activity patterns in the brain is the basis for adaptive behaviour. Understanding the mechanism by which the brain's hundred billion neurons and hundred trillion synapses manage to produce such a range of cortical configurations in a flexible manner remains a fundamental problem in neuroscience. One plausible solution is the involvement of universal mechanisms of emergent complex phenomena evident in dynamical systems poised near a critical point of a second-order phase transition. We review recent theoretical and empirical results supporting the notion that the brain is naturally poised near criticality, as well as its implications for better understanding of the brain

Predicting cell types and genetic variations contributing to disease by combining GWAS and epigenetic data

Genome-wide association studies (GWASs) identify single nucleotide polymorphisms (SNPs) that are enriched in individuals suffering from a given disease. Most disease-associated SNPs fall into non-coding regions, so that it is not straightforward to infer phenotype or function; moreover, many SNPs are in tight genetic linkage, so that a SNP identified as associated with a particular disease may not itself be causal, but rather signify the presence of a linked SNP that is functionally relevant to disease pathogenesis. Here, we present an analysis method that takes advantage of the recent rapid accumulation of epigenomics data to address these problems for some SNPs. Using asthma as a prototypic example; we show that non-coding disease-associated SNPs are enriched in genomic regions that function as regulators of transcription, such as enhancers and promoters. Identifying enhancers based on the presence of the histone modification marks such as H3K4me1 in different cell types, we show that the location of enhancers is highly cell-type specific. We use these findings to predict which SNPs are likely to be directly contributing to disease based on their presence in regulatory regions, and in which cell types their effect is expected to be detectable. Moreover, we can also predict which cell types contribute to a disease based on overlap of the disease-associated SNPs with the locations of enhancers present in a given cell type. Finally, we suggest that it will be possible to re-analyze GWAS studies with much higher power by limiting the SNPs considered to those in coding or regulatory regions of cell types relevant to a given disease

Feasibility of MRI-guided large-core-needle biopsy of suspiscious breast lesions at 3 T

The feasibility of large-core-needle magnetic resonance imaging (MRI)-guided breast biopsy at 3 T was assessed. Thirty-one suspicious breast lesions shown only by MRI were detected in 30 patients. Biopsy procedures were performed in a closed-bore 3-T clinical MR system on a dedicated phased-array breast coil with a commercially available add-on stereotactic biopsy device. Tissue sampling was technically successful in 29/31 (94%) lesions. Median lesion size (n = 29) was 9 mm. Histopathological analysis showed 19 benign lesions (66%) and one inconclusive biopsy result (3%). At follow-up of these lesions, 15 lesions showed no malignancy, no information was available in three patients and two lesions turned out to be malignant (one lesion at surgical excision 1 month after biopsy and one lesion at a second biopsy because of a more malignant enhancement curve at 12-months follow-up MRI). Nine biopsy results showed a malignant lesion (31%) which were all surgically removed. No complications occurred. MRI-guided biopsy at 3 T is a safe and effective method for breast biopsy in lesions that are occult on mammography and ultrasound. Follow-up MRI at 6 months after the biopsy should be performed in case of a benign biopsy result

Density distributions, magnetic field structures and fragmentation in high-mass star formation

Methods: Observing the large pc-scale Stokes I mm dust continuum emission with the IRAM 30m telescope and the intermediate-scale (<0.1pc) polarized submm dust emission with the Submillimeter Array toward a sample of 20 high-mass star-forming regions allows us to quantify the dependence of the fragmentation behaviour of these regions depending on the density and magnetic field structures. Results: We infer density distributions n~r^{-p} of the regions with typical power-law slopes p around ~1.5. There is no obvious correlation between the power-law slopes of the density structures on larger clump scales (~1pc) and the number of fragments on smaller core scales (<0.1pc). Comparing the large-scale single-dish density profiles to those derived earlier from interferometric observations at smaller spatial scales, we find that the smaller-scale power-law slopes are steeper, typically around ~2.0. The flattening toward larger scales is consistent with the star-forming regions being embedded in larger cloud structures that do not decrease in density away from a particular core. Regarding the magnetic field, for several regions it appears aligned with filamentary structures leading toward the densest central cores. Furthermore, we find different polarization structures with some regions exhibiting central polarization holes whereas other regions show polarized emission also toward the central peak positions. Nevertheless, the polarized intensities are inversely related to the Stokes I intensities. We estimate magnetic field strengths between ~0.2 and ~4.5mG, and we find no clear correlation between magnetic field strength and the fragmentation level of the regions. Comparison of the turbulent to magnetic energies shows that they are of roughly equal importance in this sample. The mass-to-flux ratios range between ~2 and ~7, consistent with collapsing star-forming regions.Comment: Accepted for Astronomy & Astrophysics, 14 pages, 14 figures plus appendices, also download option at https://www2.mpia-hd.mpg.de/homes/beuther/papers.htm

Different Evolutionary Stages in the Massive Star Forming Region S255 Complex

To understand evolutionary and environmental effects during the formation of high-mass stars, we observed three regions of massive star formation at different evolutionary stages that reside in the same natal molecular cloud. Methods. The three regions S255IR, S255N and S255S were observed at 1.3 mm with the Submillimeter Array (SMA) and followup short spacing information was obtained with the IRAM 30m telescope. Near infrared (NIR) H + K-band spectra and continuum observations were taken for S255IR with VLT-SINFONI to study the different stellar populations in this region. The combination of millimeter (mm) and near infrared data allow us to characterize different stellar populations within the young forming cluster in detail. While we find multiple mm continuum sources toward all regions, their outflow, disk and chemical properties vary considerably. The most evolved source S255IR exhibits a collimated bipolar outflow visible in CO and H2 emission, the outflows from the youngest region S255S are still small and rather confined in the regions of the mm continuum peaks. Also the chemistry toward S255IR is most evolved exhibiting strong emission from complex molecules, while much fewer molecular lines are detected in S255N, and in S255S we detect only CO isotopologues and SO lines. Also, rotational structures are found toward S255N and S255IR. Furthermore, a comparison of the NIR SINFONI and mm data from S255IR clearly reveal two different (proto) stellar populations with an estimated age difference of approximately 1 Myr. A multi-wavelength spectroscopy and mapping study reveals different evolutionary phases of the star formation regions. We propose the triggered outside-in collapse star formation scenario for the bigger picture and the fragmentation scenario for S255IR.Comment: 23 pages,25 figures, accepted by A&

Direct Observation of the Dynamics of Latex Particles Confined inside Thinning Water-Air Films

The dynamics of micrometer-size polystyrene latex particles confined in thinning foam films was investigated by microscopic interferometric observation. The behavior of the entrapped particles depends on the mobility of the film surfaces, the particle concentration, hydrophobicity, and rate of film formation. When the films were stabilized by sodium dodecyl sulfate, no entrapment of particles between the surfaces was possible. When protein was used as a stabilizer, a limited number of particles were caught inside the film area due to the decreased mobility of the interfaces. In this case, extraordinary long-ranged (>100 Ìm) capillary attraction leads to two-dimensional (2D) particle aggregation. A major change occurs when the microspheres are partially hydrophobized by the presence of cationic surfactant. After the foam films are opened and closed a few times, a layer of particles simultaneously adsorbed to the two interfaces is formed, which sterically inhibits any further film opening and thinning. The particles within this layer show an excellent 2D hexagonal ordering. The experimental data are relevant to the dynamics of defects in coating films, Pickering emulsions, and particle assembly into 2D arrays