Assessment and zoning of the urban area according to the level of heavy metal pollution

Urban area ranking according to the level of heavy metal pollution with identification of four areas in the territory of the city of Kazan was carried out: the 1st - Derbyshki; the 2 nd - Teplocontrol; the 3 rd - Gorki; the 4th - the Kirovsky district. Pollution of snow cover in the city territory was determined by pollution coefficients calculated with application of Maximum Permissible Concentration (MPC) of chemical substances in ambient waters for household and amenity water use. The entire territory of the city of Kazan is mildly polluted by Mn, as far as other metals (Cd, Pb, Cu, Zn, Co, Ni, Cr) are concerned, the soil pollution category varies with areas. Metal content in the hair of the children living in these areas is an informative additional method to assess the present ecological situation in certain territories

COMPOSITION AND INFECTION OF INTERMEDIATE AND DEFINITIVE HOSTS OF ECHINOCOCCUS MULTILOCULARIS (LEUCKART, 1858) IN BASIN OF LAKE BAIKAL (BURYATIA)

Based the available data and our own researches materials on Echinococcus multilocularis infection of wild animals in the basin of Lake Baikal (Republic of Buryatia) are presented here. E. multilocularis circulated by the circuit: red fox, common wolf (definitive hosts) - rodents (intermediate hosts) - red fox, common wolf (definitive hosts). The range of intermediate hosts in wild fauna includes rodents of family: Muridae (brown rat), Сricetids (muskrat, northern red-backed vole, Mongolian gerbil), Squirrels (long-tailed ground squirrel)

Recombinant Retroviral Particles: Technology of Poduction and Application as Positive Controls for PCR Diagnostics of Dangerous Viral Infections

Objective. Construction of positive control samples based on recombinant retroviral particles and their application in RT-PCR diagnostic assays for RNA detection of agents of dangerous and particularly dangerous viral infections.Materials and methods. Molecular biological, genetic engineering, and immunological methods were used: polymerase chain reaction, restriction, ligation, cloning, transformation, transfection, flow cytometry.Results and discussion. Technology of positive control samples producing based on recombinant virions has been developed and tested. It includes construction of retroviral vector with cloned diagnostic sequence of the viral genome; obtaining a packaging cell line producing chimeric retroviral particles; determination of recombinant virions titer by flow cytometry and polymerase chain reaction; application of the obtained preparation as a control sample for PCR diagnostics of infectious agents. Positive controls based on retroviral vectors as carriers of genomic RNA fragments of pathogenic viruses were used in the development of PCR diagnostic kits for dangerous and particularly dangerous viral infections. Their application increased the kits quality and made it possible to exclude the work with concentrated hazardous infectious agents (Lassa virus, tick-borne encephalitis virus, lymphocytic choriomeningitis virus, Puumala virus)

Peculiarities of chidren's risk assessment on ingestion of chemicals with drinking water

© 2010 RJPBCS.Results of the noncarcinogenic health risk assessment for the child population living in different districts of the city of Kazan are given in the article. The risk assessment was carried out for the peroral route of ingestion with the account of standard and regional exposure factors. Risk level analysis with application of local factors (particularly at the level of 95% pers.) and age differences in exposure to the chemicals ingested with drinking water showed that application of standard values resulted in underestimation of the actual health risk for the child population

Heavy metals as criteria of health and ecological well-being of the urban environment

© 2018 Elsevier GmbH The study of the content of Pb, Cd, Ni, Zn, Mn, Cr, and Cu in biological media (the hair) of children living in the zones of the city of Kazan with different pollution levels was carried out. The identification of the zones in the city of Kazan was performed on the basis of the snow cover and soils pollution with heavy metals, which are natural accumulators of chemical substances and heavy metals (HM). Statistically significant differences (р < 0.01) in the content of certain metals in the hair, lead and cadmium in particular, were revealed in children living in the technologically polluted zone (Teplocontrol). Microelement composition of the hair in children with respiratory diseases (RD) varied widely in the content of lead (р < 0.05), and a statistically significantly lower level of zinc (р < 0.01) and copper (р < 0.05) compared with all the rest groups of children was determined in genitourinary diseases (GUD). However, relatively high values of toxic elements in the control zone show that the ecological status of the city and region is instable, and implies additional measures of the environmental monitoring and activities on chemical safety in certain city zones

QUINQUESERIALIS QUINQUESERIALIS - SPECIFIC PARASITE OF MUSKRAT, AND ITS INFLUENCE ON SOME HEMATOLOGIC PARAMETERS OF THE HOST

Data on infection of muskrat by trematoda species Quinqueserialis quinqueserialis in the Selenga River Delta are presented in this paper. The indexes of infection of muskrat by parasite in 2011 are similar to those in 1981 and 1982. The study is the first attempt to assess the influence of infestation on structure and. quantity leucocytes of blood of infected muskrat in Lake Baikal basin

DANSSino: a pilot version of the DANSS neutrino detector

DANSSino is a reduced pilot version of a solid-state detector of reactor antineutrinos (to be created within the DANSS project and installed under the industrial 3 GW(th) reactor of the Kalinin Nuclear Power Plant -- KNPP). Numerous tests performed at a distance of 11 m from the reactor core demonstrate operability of the chosen design and reveal the main sources of the background. In spite of its small size (20x20x100 ccm), the pilot detector turned out to be quite sensitive to reactor antineutrinos, detecting about 70 IBD events per day with the signal-to-background ratio about unity.Comment: 16 pages, 11 figures, 3 tables. arXiv admin note: substantial text overlap with arXiv:1304.369

Significance of individual management plan for the patients with hereditary lymphangioedema during pregnancy and lactation, and in newborns

Hereditary angioedema (HAE) is a genetically caused orphan disease with a high risk of developing life-threatening attacks, thus requiring availability for up-to-date information on this problem for the doctors of any specialties. A limited number of observations determine the value of the analysis for each clinical case. Many facets of clinical manifestations, a list of predisposing and triggering factors, as well as limitations of some diagnostic and therapeutic algorithms, require the development of individual management schemes under distinct clinical situations. In this paper, we present the unique clinical cases with certain limitations, describing unexpected onset of the disease in the course of pregnancy, management aspects during delivery and post-delivery periods in a women with a previously confirmed HAE diagnosis. Adapted diagnostic algorithms of postnatal diagnostic verification are presented for children with burdened genetic history. We express some assumptions about involvement of a multidisciplinary team of specialists, personalized approach to building a management plan with an “online” correction depending on observation stage of НАЕ patient

Impact of obesity on the incidence of purulent-septic complications in the wound after cesarean section

This study was based on a retrospective analysis of 1,390 deliveries by cesarean section. The patients were divided into groups according to the degrees of obesity (I-III degree). The peculiarities of suture management in obese patients and the incidence of infectious complications were studied.Данное исследование основано на ретроспективном анализе 1390 родоразрешений женщин путем операции кесарева сечения. Пациентки были разделены на группы согласно степеням ожирения (I-III степени). Были изучены особенности ведения шва у пациенток с ожирением, частота возникновения инфекционных осложнений

Cкринінг діуретичної активності в ряду нових похідних оксамінових кислот

The screening study of oxamic acid derivatives and their influence on the excretory function of the kidneys in Wistar white rats under conditions of water load has been carried out. The compounds under research are white crystalline substances of the basic character, odourless, with a distinct melting point, soluble in polar organic solvents and mineral acids. The structure and purity of the substances studied have been confirmed by modern physicochemical methods of elemental, IR and PMR spectral, chromatographic analysis and qualitative reactions. The analysis of the effect of these substances on the excretory function of the kidneys have been carried out on white male rats by the method of E.B. Berkhin using a number of animals with 7 rats in each group. The activity of the compounds studied was compared with the activity of well-known drugs – hypothiazide, furosemide and adiurecrine. A number of compounds caused decrease in urine output in animals. The diuretic activity of the compounds was influenced by the chemical characteristics of the substituent and its location. As a result of the research performed substances increasing diuresis with the diuretic activity exceeding that of the reference medicine – hypothiazide – have been found. Substituted amides of arenesulphonyloxamic acid are a promising group of compounds for further pharmacological study with the aim of development of new medicines with the diuretic activity on their basis.Проведены скрининговые исследования производных оксаминовых кислот и их влияния на выделительную функцию почек у белых крыс линии Вистар в условиях водной нагрузки. Исследуемые соединения представляют собой белые кристаллические вещества основного характера без запаха, с четкой температурой плавления, растворимые в полярных органических растворителях и минеральных кислотах. Строение и чистота исследуемых веществ подтверждены современными физико-химическими методами элементного, ИК-, ПМР-спектрального, хроматографического анализов и качественными реакциями. Изучение влияния данных веществ на выделительную функцию почек проводили на белых крысах-самцах по методу Е.Б.Берхина, использовали серии животных по 7 крыс в каждой группе. Активность исследуемых соединений сравнивали с активностью хорошо известных лекарственных средств – гипотиазида, фуросемида и адиурекрина. В результате проведенных исследований выявлены вещества, вызывающие увеличение диуреза, превышающие по диуретической активности эталонный препарат сравнения гипотиазид. Ряд соединений вызывал уменьшение диуреза у животных. На диуретическую активность изучаемых соединений влияла как химическая особенность заместителя, так и его расположение. Замещенные амиды аренсульфонилоксаминовых кислот являются перспективной группой соединений для дальнейшего фармакологического изучения с целью создания на их основе лекарственных препаратов с диуретическими свойствами.Проведені скринінгові дослідження похідних оксамінових кислот та їх впливу на видільну функцію нирок у білих щурів лінії Вістар в умовах водного навантаження. Досліджувані сполуки являють собою білі кристалічні речовини основного характеру без запаху, з чіткою температурою плавлення, розчинні в полярних органічних розчинниках, мінеральних кислотах. Будова та чистота досліджуваних речовин підтверджені сучасними фізико-хімічними методами елементного, ІЧ-, ПМР-спектрального, хроматографічного аналізів та якісними реакціями. Вивчення впливу даних речовин на видільну функцію нирок проводили на білих щурах-самцях за методом Є.Б.Берхіна, використовували серії тварин по 7 щурів у кожній групі. Активність сполук, які досліджувались, порівнювали з активністю добре відомих лікарських засобів – гіпотіазиду, фуросеміду та адіурекрину. В результаті проведених досліджень виявлені речовини, які викликають збільшення діурезу, що перевищує по діуретичній активності еталонний препарат порівняння – гіпотіазид. Ряд сполук викликав зменшення діурезу у тварин. На діуретичну активність сполук, що вивчались, впливала як хімічна особливість замісника, так і його розташування. Заміщені аміди аренсульфонілоксамінових кислот є перспективною групою сполук для подальшого фармакологічного вивчення з метою створення на їх основі лікарських препаратів з діуретичними властивостями