The use of color infrared aerial photography in determining salt marsh vegetation and delimiting man-made structures of Lynnhaven Bay, Virginia

Color infrared aerial photography was found to be superior to color aerial photography in an ecological study of Lynnhaven Bay, Virginia. The research was divided into three phases: (1) Determination of the feasibility of correlating color infrared aerial photography with saline wetland species composition and zonation patterns, (2) determination of the accuracy of the aerial interpretation and problems related to the aerial method used; and (3) comparison of developed with undeveloped areas along Lynnhaven Bay's shoreline. Wetland species composition and plant community zonation bands were compared with aerial infrared photography and resulted in a high degree of correlation. Problems existed with changing physical conditions; time of day, aircraft angle and sun angle, making it necessary to use several different characteristics in wetland species identification. The main characteristics used were known zonation patterns, textural signatures and color tones. Lynnhaven Bay's shoreline was 61.5 percent developed

Why the Universe Started from a Low Entropy State

We show that the inclusion of backreaction of massive long wavelengths imposes dynamical constraints on the allowed phase space of initial conditions for inflation, which results in a superselection rule for the initial conditions. Only high energy inflation is stable against collapse due to the gravitational instability of massive perturbations. We present arguments to the effect that the initial conditions problem {\it cannot} be meaningfully addressed by thermostatistics as far as the gravitational degrees of freedom are concerned. Rather, the choice of the initial conditions for the universe in the phase space and the emergence of an arrow of time have to be treated as a dynamic selection.Comment: 12 pages, 2 figs. Final version; agrees with accepted version in Phys. Rev.

5 S,15 S-Dihydroperoxyeicosatetraenoic Acid (5,15-diHpETE) as a Lipoxin Intermediate: Reactivity and Kinetics with Human Leukocyte 5-Lipoxygenase, Platelet 12-Lipoxygenase, and Reticulocyte 15-Lipoxygenase-1.

The reaction of 5 S,15 S-dihydroperoxyeicosatetraenoic acid (5,15-diHpETE) with human 5-lipoxygenase (LOX), human platelet 12-LOX, and human reticulocyte 15-LOX-1 was investigated to determine the reactivity and relative rates of producing lipoxins (LXs). 5-LOX does not react with 5,15-diHpETE, although it can produce LXA4 when 15-HpETE is the substrate. In contrast, both 12-LOX and 15-LOX-1 react with 5,15-diHpETE, forming specifically LXB4. For 12-LOX and 5,15-diHpETE, the kinetic parameters are kcat = 0.17 s-1 and kcat/ KM = 0.011 μM-1 s-1 [106- and 1600-fold lower than those for 12-LOX oxygenation of arachidonic acid (AA), respectively]. On the other hand, for 15-LOX-1 the equivalent parameters are kcat = 4.6 s-1 and kcat/ KM = 0.21 μM-1 s-1 (3-fold higher and similar to those for 12-HpETE formation by 15-LOX-1 from AA, respectively). This contrasts with the complete lack of reaction of 15-LOX-2 with 5,15-diHpETE [Green, A. R., et al. (2016) Biochemistry 55, 2832-2840]. Our data indicate that 12-LOX is markedly inferior to 15-LOX-1 in catalyzing the production of LXB4 from 5,15-diHpETE. Platelet aggregation was inhibited by the addition of 5,15-diHpETE, with an IC50 of 1.3 μM; however, LXB4 did not significantly inhibit collagen-mediated platelet activation up to 10 μM. In summary, LXB4 is the primary product of 12-LOX and 15-LOX-1 catalysis, if 5,15-diHpETE is the substrate, with 15-LOX-1 being 20-fold more efficient than 12-LOX. LXA4 is the primary product with 5-LOX but only if 15-HpETE is the substrate. Approximately equal proportions of LXA4 and LXB4 are produced by 12-LOX but only if LTA4 is the substrate, as described previously [Sheppard, K. A., et al. (1992) Biochim. Biophys. Acta 1133, 223-234]

The Size Distribution of Trans-Neptunian Bodies

[Condensed] We search 0.02 deg^2 for trans-Neptunian objects (TNOs) with m<=29.2 (diameter ~15 km) using the ACS on HST. Three new objects are discovered, roughly 25 times fewer than expected from extrapolation of the differential sky density Sigma(m) of brighter objects. The ACS and other recent TNO surveys show departures from a power law size distribution. Division of the TNO sample into ``classical Kuiper belt'' (CKB) and ``Excited'' samples reveals that Sigma(m) differs for the two populations at 96% confidence. A double power law adequately fits all data. Implications include: The total mass of the CKB is ~0.010 M_Earth, only a few times Pluto's mass, and is predominately in the form of ~100 km bodies. The mass of Excited objects is perhaps a few times larger. The Excited class has a shallower bright-end size distribution; the largest objects, including Pluto, comprise tens of percent of the total mass whereas the largest CKBOs are only ~2% of its mass. The predicted mass of the largest Excited body is close to the Pluto mass; the largest CKBO is ~60 times less massive. The deficit of small TNOs occurs for sizes subject to disruption by present-day collisions, suggesting extensive depletion by collisions. Both accretion and erosion appearing to have proceeded to more advanced stages in the Excited class than the CKB. The absence of distant TNOs implies that any distant (60 AU) population must have less than the CKB mass in the form of objects 40 km or larger. The CKB population is sparser than theoretical estimates of the required precursor population for short period comets, but the Excited population could be a viable precursor population.Comment: Revised version accepted to the Astronomical Journal. Numerical results are very slightly revised. Implications for the origins of short-period comets are substantially revised, and tedious material on statistical tests has been collected into a new Appendi

Space Station Facilities and Operations at the Kennedy Space Center

The Spaca Station Program potentially will a significant Impact on the Kennedy Space Canter Current and past programs have had their major Impact on KSC In the prelaunch and launch operations area with program support, subsequent to liftoff, generally performed at other locations. The Space Station Program also will Impact pre- launch and launch operations at KSC but, due to Its proposed 10-year operational lifetime, KSC also could have a significant role in support of the operational program. Support operations of the Space Station Program may be performed elsewhere; however, centralizing them at KSC has a number of attractive features which warrant study

A novel protein isoform of the RON tyrosine kinase receptor transforms human pancreatic duct epithelial cells.

The MST1R gene is overexpressed in pancreatic cancer producing elevated levels of the RON tyrosine kinase receptor protein. While mutations in MST1R are rare, alternative splice variants have been previously reported in epithelial cancers. We report the discovery of a novel RON isoform discovered in human pancreatic cancer. Partial splicing of exons 5 and 6 (P5P6) produces a RON isoform that lacks the first extracellular immunoglobulin-plexin-transcription domain. The splice variant is detected in 73% of xenografts derived from pancreatic adenocarcinoma patients and 71% of pancreatic cancer cell lines. Peptides specific to RON P5P6 detected in human pancreatic cancer specimens by mass spectrometry confirm translation of the protein isoform. The P5P6 isoform is found to be constitutively phosphorylated, present in the cytoplasm, and it traffics to the plasma membrane. Expression of P5P6 in immortalized human pancreatic duct epithelial (HPDE) cells activates downstream AKT, and in human pancreatic epithelial nestin-expressing cells, activates both the AKT and MAPK pathways. Inhibiting RON P5P6 in HPDE cells using a small molecule inhibitor BMS-777607 blocked constitutive activation and decreased AKT signaling. P5P6 transforms NIH3T3 cells and induces tumorigenicity in HPDE cells. Resultant HPDE-P5P6 tumors develop a dense stromal compartment similar to that seen in pancreatic cancer. In summary, we have identified a novel and constitutively active isoform of the RON tyrosine kinase receptor that has transforming activity and is expressed in human pancreatic cancer. These findings provide additional insight into the biology of the RON receptor in pancreatic cancer and are clinically relevant to the study of RON as a potential therapeutic target

On detecting terrestrial planets with timing of giant planet transits

The transits of a planet on a Keplerian orbit occur at time intervals exactly equal to the period of the orbit. If a second planet is introduced the orbit is not Keplerian and the transits are no longer exactly periodic. We compute the magnitude of these variations in the timing of the transits, dt. We investigate analytically several limiting cases: (i) interior perturbing planets with much smaller periods; (ii) exterior perturbing planets on eccentric orbits with much larger periods; (iii) both planets on circular orbits with arbitrary period ratio but not in resonance; and (iv) planets on initially circular orbits locked in resonance. Case (iv) is perhaps the most interesting case since some systems are known to be in resonances and the perturbations are the largest. As long as the perturber is more massive than the transiting planet, the timing variations would be of order of the period regardless of the perturber mass! For lighter perturbers, we show that the timing variations are smaller than the period by the perturber to transiting planet mass ratio. An earth mass planet in 2:1 resonance with a 3-day period transiting planet (e.g. HD 209458b) would cause timing variations of order 3 minutes, which would be accumulated over a year. These are easily detectable with current ground-based measurements. For the case of both planets on eccentric orbits, we compute numerically the transit timing variations for several cases of known multiplanet systems assuming they were edge-on. Transit timing measurements may be used to constrain the masses and radii of the planetary system and, when combined with radial velocity measurements, to break the degeneracy between mass and radius of the host star. (abstract truncated)Comment: 21 pages, 9 figures, submitted to MNRA

CORRELATION OF FINE STRUCTURE AND PHYSIOLOGY OF THE INNERVATION OF SMOOTH MUSCLE IN THE GUINEA PIG VAS DEFERENS

An electron microscope study of the innervation of smooth muscle of the guinea pig vas deferens was undertaken in order to find a structural basis for recent electrophysiological observations. The external longitudinal muscle coat was examined in transverse section. Large areas of the surfaces of adjacent muscle cells were 500 to 800 A apart. Closer contacts were rare. A special type of close contact suggested cytoplasmic transfer between neighbouring cells. Groups of non-myelinated axons from ganglia at the distal end of the hypogastric nerve ramified throughout the muscle. Some small axon bundles and single axons lay in narrow fissures within closely packed muscle masses. Many axons contained "synaptic vesicles." About 25 per cent of the muscle fibres in the plane of section were within 0.25 µ of a partly naked axon; of these 15 per cent were within 500 A of the axon, and about 1 per cent made close contact (200 A) with a naked axon. It is unlikely that every muscle fibre is in close contact with an axon, and it is not possible for every fibre to have many such contacts. Muscle fibres are probably activated by both diffusion of transmitter from naked portions of axons a fraction of a micron distant, and electrotonic spread of activity from neighbouring cells

The potential for circular dichroism as an additional facile and sensitive method of monitoring low-molecular-weight heparins and heparinoids

The ultraviolet circular dichroism (CD) spectra of commercial low-molecular-weight heparins, heparinoids and other anticoagulant preparations have been recorded between 180 and 260 nm. Principal component analysis of the spectra allowed their differentiation into a number of groups related to the means of their production reflecting the structural changes introduced by each process. The findings suggest that CD provides a complementary technique for the rapid analysis of heparin preparations