Characteristics of Staphylococcus aureus infections to consider in designing an effective vaccine.

_Staphylococcus aureus_ is a very versatile and adaptable microorganism. It can potentially infect virtually any host tissue. Given the appropriate conditions it can become a life-threatening pathogen, or a commensal colonizer of the nose. Extensive antibiotic use for infection control facilitated the rise of antibiotic resistance, stressing the need for alternate forms of control. Vaccine efforts in other pathogens have proved successful, but so far _S. aureus_ candidate vaccines have not been as effective. Here we review _S. aureus_ factors involved in pathogenesis that could help develop a successful vaccine, like host nasal colonization and immune evasion factors. An effective multicomponent vaccine could incorporate antigenic fragments from several _S. aureus_ proteins, preferably involved in colonization, immune evasion and/or toxicity

An Analysis of Water Leakages in Series Pipelines Using the Rigid Column Model

[EN] Water leakages are assessed with an extended period simulation (traditional analysis) in water distribution networks. However, when rapid manoeuvres regulate valves, the conventional analysis proves unsuitable for representing the water volume of leaks. Under these circumstances, the Rigid Water Column Model can be utilised to accurately compute the quantity of water volume leaks. This method has been applied to single and parallel pipelines to predict water leakages, considering the opening and closure manoeuvres of regulating valves. This research develops governing equations using the Rigid Column Model to predict water volume leaks in a general scheme of series pipelines, accounting for the opening and closure manoeuvres in regulating valves. The practical application involves two branches of a series pipeline with internal pipe diameters of 0.45 and 0.25 m and pipe lengths of 1200 and 1400 m, respectively. The results of this practical application demonstrate the accuracy of the Rigid Column Model and its relevance in real-world scenarios. For instance, the model shows that traditional analysis can overestimate water volume leaks by approximately 72% when a closure manoeuvre regulates valves for 200 s and by about 28% for 30 s, highlighting the need for a more accurate method.Fuertes-Miquel, VS.; Coronado-Hernández, OE.; Arrieta-Pastrana, A. (2024). An Analysis of Water Leakages in Series Pipelines Using the Rigid Column Model. Fluids. 9(9):1-15. https://doi.org/10.3390/fluids90902011159

Assessment of potential prebiotic activity of pineapple by-products (peels and stems) extracts and maintenance of bioavailability through in vitro gastrointestinal tract system

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Neuropsychological analysis of anxiety and executive control of motor patterns in athletes and non-athletes.

Introduction: Even simple tapping tasks require cognitive processes. Some variants of the Finger Tapping Test (FTT) may reveal cognitive aspects associated with frontal processing, including executive functions such as inhibition, or emotional aspects such as anxiety. A context of particular interest for the application of cognitive-motor-anxiety interactions is sports. Although athletes generally exhibit better anxiety levels, they may experience heightened anxiety before important competitions. The problem lies in determining whether the application of anxiety control techniques can be useful in pre-competition situations, given the lack of quick and easy methods to detect if an athlete is experiencing anxiety at a particular moment. Methods: This exploratory study evaluated anxiety using online versions of questionnaires (ISRA, the Competitive State Anxiety Inventory-2, and STAI) and applied a variant of the FTT to 204 participants, both athletes and non-athletes. The scores were compared and correlated. Results: Athletes exhibited lower general anxiety and greater cognitive resistance to interference (better cognitive inhibition). Non-athletes displayed a particular parameter in the FTT variant that differed from the one obtained by athletes and exhibited higher anxiety levels. In the athletes’ group only, anxiety was correlated with a specific parameter of the FTT task. Discussion: Our conclusion is that this parameter holds potential relevance in elite sports performance to detect if an athlete is experiencing anxiety. It could be of particular interest in psychological interventions in sports. Further investigation is warranted to fully explore this potential

Sex-partitioning of the <i>Plasmodium falciparum</i> stage V gametocyte proteome provides insight into <i>falciparum</i>-specific cell biology

One of the critical gaps in malaria transmission biology and surveillance is our lack of knowledge about Plasmodium falciparum gametocyte biology, especially sexual dimorphic development and how sex ratios that may influence transmission from the human to the mosquito. Dissecting this process has been hampered by the lack of sex-specific protein markers for the circulating, mature stage V gametocytes. The current evidence suggests a high degree of conservation in gametocyte gene complement across Plasmodium, and therefore presumably for sex-specific genes as well. To better our understanding of gametocyte development and subsequent infectiousness to mosquitoes, we undertook a Systematic Subtractive Bioinformatic analysis (filtering) approach to identify sex-specific P. falciparum NF54 protein markers based on a comparison with the Dd2 strain, which is defective in producing males, and with syntenic male and female proteins from the reanalyzed and updated P. berghei (related rodent malaria parasite) gametocyte proteomes. This produced a short list of 174 male- and 258 female-enriched P. falciparum stage V proteins, some of which appear to be under strong diversifying selection, suggesting ongoing adaptation to mosquito vector species. We generated antibodies against three putative female-specific gametocyte stage V proteins in P. falciparum and confirmed either conserved sex-specificity or the lack of cross-species sex-partitioning. Finally, our study provides not only an additional resource for mass spectrometry-derived evidence for gametocyte proteins but also lays down the foundation for rational screening and development of novel sex-partitioned protein biomarkers and transmission-blocking vaccine candidates

Camel genetic resources conservation through tourism: A key sociocultural approach of camelback leisure riding

Camels are exotic elements, which can be comprised within adventure travel companies promoting ecotourism activities. Such recreations contribute to sustainable livelihoods for local communities and educational empowerment towards nature and its conservation. At present, some local camel breeds’ survival reduces to this animal-based leisure industry and its reliability to perform and promote customized services accurately. By conducting an on-site questionnaire to customers participating in camelback riding tours, we assessed the motivational factors affecting participation, satisfaction, and loyalty in this tourism segment that may have made it socially differentiated. The sixfold combination of staff performance, culture geography, diverse and humane close interaction, camel behavior and performance, sociotemporal context, and positive previous experience involves the elemental dimensions that explain customer satisfaction and return intention probability within this entertainment business. Customer knowledge is essential for stakeholders to build personalized riding experiences and align profits with environmental sustainability and biodiversity mainstream concerns into their everyday operations. In turn, domestic camel tourist rides could be managed as a viable path to nature conservation by helping endangered local breeds to avoid their functional devaluation and potential extinction

Effect of research impact on emerging camel husbandry, welfare and social-related awareness

The lack of applied scientific research on camels, despite them being recognized as production animals, compels the reorganization of emerging camel breeding systems with the aim of achieving successful camel welfare management strategies all over the world. Relevant and properly-framed research widely impacts dissemination of scientific contents and drives public willingness to enhance ethically acceptable conditions for domestic animals. Consumer perception of this livestock industry will improve and high-quality products will be obtained. This paper draws on bibliometric indicators as promoting factors for camel-related research advances, tracing historical scientific publications indexed in ScienceDirect directory from 1880–2019. Camel as a species did not affect Journal Citation Reports (JCR) impact (p &gt; 0.05) despite the journal, author number, corresponding author origin, discipline and publication year affecting it (p &lt; 0.001). Countries with traditionally well-established camel farming are also responsible for the papers with the highest academic impact. However, camel research advances may have only locally and partially influenced welfare related laws, so intentional harming acts and basic needs neglect may persist in these species. A sustainable camel industry requires those involved in camel research to influence business stakeholders and animal welfare advocacies by highlighting the benefits of camel wellbeing promotion, co-innovation partnership establishment and urgent enhancement of policy reform

Síntesis y evaluación citotoxica de derivados halogenados y peracetylados de nucleósidos en celulas de cancer de mama

Objectives. To make the synthesis of halogenated derivatives on the nitrogenous base and their respective acyl ester and amide type derivatives for all hydroxyl and amine groups of the uridine and cytarabine nucleosides, and evaluate cytotoxicity against breast cancer cell line. Methods. First, it was accomplished the halogenation reaction on the 5-position of the nitrogenous base, subsequently, the ester and amide derivatives were performed for all hydroxyl and amine group present in the nucleosides. Besides, the uridine acetonide derivatives as prepared by acid catalysis. The products were characterized by nuclear magnetic resonance spectroscopy (1H RMN y 13C RMN) and mass spectrometry in positive mode by direct injection. Derivatives were evaluated in Chinese hamster ovary (CHO-K1) and human breast cancer (MCF-7) cell lines. Results. The four derivatives were obtained with chlorine and bromine for the uridine and cytarabine, respectively, their respective per-acetylated derivatives, the per-acetylated nucleoside and the uridine acetonide; the compounds were obtained with efficiency over 90%. The per-acetylated nucleosides and the halogenated and per-acetylated derivatives did not show inhibitory effects on cell viability in MCF-7 cell line. However, the per-acetylated and halogenated derivatives presented a higher cytotoxic activity than their respective per-acetylated nucleoside. The uridine 3’,4’-acetonide showed a significant cytotoxicity on both cell lines. Conclusions. The per-acetylated nucleoside, and the respective halogenated derivatives with chlorine and bromine were obtained with high yields, nevertheless, these compounds did not exhibit a significant anti-proliferative activity (p˂0.05), possibly due to a low intra-cellular activation.Objetivos: Sintetizar derivados halogenados sobre la base nitrogenada, sus respectivos derivados tipo éster o amida de todos los grupos hidroxilo y amina presentes en los nucleósidos uridina y citarabina, y evaluar su actividad citotóxica sobre una línea celular de cáncer de mama. Metodología: primero se realizó la reacción de halogenación en la posición 5 de la base nitrogenada, posteriormente se formaron los ésteres y amidas de todos los grupos hidroxilos y amino presentes en los nucleósidos. Además, se preparó el derivado acetónido con catálisis ácida. Los compuestos se caracterizaron por espectroscopía de resonancia magnética nuclear (RMN 1H y RMN 13C) y espectrometría de masas por inyección directa en modo positivo. Los derivados se evaluaron sobre líneas celulares de tumor de Ovario de Hámster Chino (CHO) y de cáncer de mamá (MCF-7). Resultados: Se obtuvieron 4 derivados mono-halogenados con cloro y bromo de la uridina y citarabina, respectivamente, sus respectivos derivados per-acetilados, los nucleósidos per-acetilados y el acetónido de la uridina; los compuestos se obtuvieron con rendimientos superiores a 90%. Los nucleósidos per-acetilados, y los derivados per-acetilados y halogenados no exhibieron una inhibición significativa de la viabilidad celular en ambas líneas celulares, sin embargo, de estos, los derivados per-acetilados y halogenados presentaron mayor actividad citotóxica que los respectivos nucleósidos per-acetilados. El derivado acetónido de la uridina mostró citotoxicidad significativa sobre ambas líneas celulares. Conclusiones: se obtuvieron los nucleósidos per-acetilados y los respectivos derivados clorados y bromados de estos, con rendimientos altos, sin embargo, estos compuestos no exhibieron una actividad anti-proliferativa significativa (p˂0,05), posiblemente debido a una baja activación intra-celular de los nucleósidos

Assessment of Peak Water Usage among Residential Consumers across Several Drinking Water Service Areas

[EN] Public drinking water service providers must comprehensively understand and effectively characterise user demands, especially during peak hours, which not only impact the maximum demand within the distribution network but also determine the dimensions of interior networks within buildings. Residential consumers show different consumption patterns based on socioeconomic factors, spatial location, climatic conditions and the consistency and quality of service delivered by public service providers. This study focused on assessing 1,317,584 users distributed across four distinct service areas in Bogota, Colombia. To achieve this, a stratified random sampling of 1233 residential subscribers was conducted and 320 reference digital Y290 Aquabus micro-meters were installed to characterise the four service areas. The installations were grouped into sets of 320 users until the entire sample of 1233 subscribers was encompassed. The results demonstrated that the rational method provided the most accurate fit for estimating the probable maximum flow rates compared to the values measured and, consequently, is the most suitable method for application within the region of interest. However, whereas the Hunter Unal method displayed a reasonable fit, it tended to underestimate the size of internal networks within buildings. The remaining methods, such as the British, square root, simultaneity, Hunter, NTC 1500 Hunter and Chilean methods, did not yield significant adjustments and tended to overestimate the probable maximum flow rates as well as the internal networks within buildings. The results indicate that, depending on the method used to calculate the probable maximum flow or design flow of the internal network, there can be a deviation factor when compared to the actual peak flow measured (real maximum flow). This deviation factor ranges from 0.79 (calculated less than measured) to 3.77 (calculated greater than measured). Additionally, a sizing case study was conducted, which involved applying all methods to a scenario involving a residential user. This study aimed to determine the variation expected in the estimation of the diameter of the supply pipe to the internal network when using the flow results from different methods. This analysis serves to conclude the research.Garzón-Orduña, AJ.; Coronado-Hernández, OE.; Ortiz, RO.; Arrieta-Pastrana, A.; Fuertes-Miquel, VS. (2024). Assessment of Peak Water Usage among Residential Consumers across Several Drinking Water Service Areas. Sustainability. 16(4):1-31. https://doi.org/10.3390/su1604160113116