474 research outputs found
Alternative mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus
2D and 3D cryo-electron microscopy, together with adsorption kinetics assays of ϕCb13 and ϕCbK phage-infected Caulobacter crescentus, provides insight into the mechanisms of infection. ϕCb13 and ϕCbK actively interact with the flagellum and subsequently attach to receptors on the cell pole. We present evidence that the first interaction of the phage with the bacterial flagellum takes place through a filament on the phage head. This contact with the flagellum facilitates concentration of phage particles around the receptor (i.e., the pilus portals) on the bacterial cell surface, thereby increasing the likelihood of infection. Phage head filaments have not been well characterized and their function is described here. Phage head filaments may systematically underlie the initial interactions of phages with their hosts in other systems and possibly represent a widespread mechanism of efficient phage propagation
Efect of magnetic Gd impurities on superconductivity in MoGe films with different thickness and morphology
We studied the effect of magnetic doping with Gd atoms on the superconducting
properties of amorphous Mo70Ge30 films. We observed that in uniform films
deposited on amorphous Ge, the pair-breaking strength per impurity strongly
decreases with film thickness initially and saturates at a finite value in
films with thickness below the spin-orbit scattering length. The variation is
likely caused by surface induced magnetic anisotropy and is consistent with the
fermionic mechanism of superconductivity suppression. In thin films deposited
on SiN the pair-breaking strength becomes zero. Possible reasons for this
anomalous response are discussed. The morphological distinctions between the
films of the two types were identified using atomic force microscopy with a
carbon nanotube tip
Barriers to enrollment in a randomized controlled trial of hydrocortisone for cardiovascular insufficiency in term and late preterm newborn infants.
ObjectiveTo analyze reasons for low enrollment in a randomized controlled trial (RCT) of the effect of hydrocortisone for cardiovascular insufficiency on survival without neurodevelopmental impairment (NDI) in term/late preterm newborns.Study designThe original study was a multicenter RCT. Eligibility: ⩾34 weeks' gestation, <72 h old, mechanically ventilated, receiving inotrope. Primary outcome was NDI at 2 years; infants with diagnoses at high risk for NDI were excluded. This paper presents an analysis of reasons for low patient enrollment.ResultsTwo hundred and fifty-seven of the 932 otherwise eligible infants received inotropes; however, 207 (81%) had exclusionary diagnoses. Only 12 infants were randomized over 10 months; therefore, the study was terminated. Contributing factors included few eligible infants after exclusions, open-label steroid therapy and a narrow enrollment window.ConclusionDespite an observational study to estimate the population, very few infants were enrolled. Successful RCTs of emergent therapy may require fewer exclusions, a short-term primary outcome, waiver of consent and/or other alternatives
Reverberation Mapping Measurements of Black Hole Masses in Six Local Seyfert Galaxies
We present the final results from a high sampling rate, multi-month,
spectrophotometric reverberation mapping campaign undertaken to obtain either
new or improved Hbeta reverberation lag measurements for several relatively
low-luminosity AGNs. We have reliably measured thetime delay between variations
in the continuum and Hbeta emission line in six local Seyfert 1 galaxies. These
measurements are used to calculate the mass of the supermassive black hole at
the center of each of these AGNs. We place our results in context to the most
current calibration of the broad-line region (BLR) R-L relationship, where our
results remove outliers and reduce the scatter at the low-luminosity end of
this relationship. We also present velocity-resolved Hbeta time delay
measurements for our complete sample, though the clearest velocity-resolved
kinematic signatures have already been published.Comment: 52 pages (AASTeX: 29 pages of text, 8 tables, 7 figures), accepted
for publication in the Astrophysical Journa
CSF from Parkinson disease Patients Differentially Affects Cultured Microglia and Astrocytes
<p>Abstract</p> <p>Background</p> <p>Excessive and abnormal accumulation of alpha-synuclein (α-synuclein) is a factor contributing to pathogenic cell death in Parkinson's disease. The purpose of this study, based on earlier observations of Parkinson's disease cerebrospinal fluid (PD-CSF) initiated cell death, was to determine the effects of CSF from PD patients on the functionally different microglia and astrocyte glial cell lines. Microglia cells from human glioblastoma and astrocytes from fetal brain tissue were cultured, grown to confluence, treated with fixed concentrations of PD-CSF, non-PD disease control CSF, or control no-CSF medium, then photographed and fluorescently probed for α-synuclein content by deconvolution fluorescence microscopy. Outcome measures included manually counted cell growth patterns from day 1-8; α-synuclein density and distribution by antibody tagged 3D model stacked deconvoluted fluorescent imaging.</p> <p>Results</p> <p>After PD-CSF treatment, microglia growth was reduced extensively, and a non-confluent pattern with morphological changes developed, that was not evident in disease control CSF and no-CSF treated cultures. Astrocyte growth rates were similarly reduced by exposure to PD-CSF, but morphological changes were not consistently noted. PD-CSF treated microglia showed a significant increase in α-synuclein content by day 4 compared to other treatments (p ≤ 0.02). In microglia only, α-synuclein aggregated and redistributed to peri-nuclear locations.</p> <p>Conclusions</p> <p>Cultured microglia and astrocytes are differentially affected by PD-CSF exposure compared to non-PD-CSF controls. PD-CSF dramatically impacts microglia cell growth, morphology, and α-synuclein deposition compared to astrocytes, supporting the hypothesis of cell specific susceptibility to PD-CSF toxicity.</p
A Revised Broad-Line Region Radius and Black Hole Mass for the Narrow-Line Seyfert 1 NGC 4051
We present the first results from a high sampling rate, multi-month
reverberation mapping campaign undertaken primarily at MDM Observatory with
supporting observations from telescopes around the world. The primary goal of
this campaign was to obtain either new or improved Hbeta reverberation lag
measurements for several relatively low luminosity AGNs. We feature results for
NGC 4051 here because, until now, this object has been a significant outlier
from AGN scaling relationships, e.g., it was previously a ~2-3sigma outlier on
the relationship between the broad-line region (BLR) radius and the optical
continuum luminosity - the R_BLR-L relationship. Our new measurements of the
lag time between variations in the continuum and Hbeta emission line made from
spectroscopic monitoring of NGC 4051 lead to a measured BLR radius of R_BLR =
1.87 (+0.54 -0.50) light days and black hole mass of M_BH = 1.73 (+0.55 -0.52)
x 10^6 M_sun. This radius is consistent with that expected from the R_BLR-L
relationship, based on the present luminosity of NGC 4051 and the most current
calibration of the relation by Bentz et al. (2009a). We also present a
preliminary look at velocity-resolved Hbeta light curves and time delay
measurements, although we are unable to reconstruct an unambiguous
velocity-resolved reverberation signal.Comment: 38 pages, 7 figures, accepted for publication in ApJ, changes from v1
reflect suggestions from anonymous refere
OGLE-2014-BLG-0289: Precise Characterization of a Quintuple-peak Gravitational Microlensing Event
We present the analysis of the binary-microlensing event OGLE-2014-BLG-0289. The event light curve exhibits five very unusual peaks, four of which were produced by caustic crossings and the other by a cusp approach. It is found that the quintuple-peak features of the light curve provide tight constraints on the source trajectory, enabling us to precisely and accurately measure the microlensing parallax πE. Furthermore, the three resolved caustics allow us to measure the angular Einstein radius θE. From the combination of πE and θE, the physical lens parameters are uniquely determined. It is found that the lens is a binary composed of two M dwarfs with masses M1 = 0.52 ± 0.04 M⊙ and M2 = 0.42 ± 0.03 M⊙ separated in projection by a⊥ = 6.4 ± 0.5 au. The lens is located in the disk with a distance of DL = 3.3 ± 0.3 kpc. The reason for the absence of a lensing signal in the Spitzer data is that the time of observation corresponds to the flat region of the light curve
The Extreme Microlensing Event OGLE-2007-BLG-224: Terrestrial Parallax Observation of a Thick-Disk Brown Dwarf
Parallax is the most fundamental technique to measure distances to
astronomical objects. Although terrestrial parallax was pioneered over 2000
years ago by Hipparchus (ca. 140 BCE) to measure the distance to the Moon, the
baseline of the Earth is so small that terrestrial parallax can generally only
be applied to objects in the Solar System. However, there exists a class of
extreme gravitational microlensing events in which the effects of terrestrial
parallax can be readily detected and so permit the measurement of the distance,
mass, and transverse velocity of the lens. Here we report observations of the
first such extreme microlensing event OGLE-2007-BLG-224, from which we infer
that the lens is a brown dwarf of mass M=0.056 +- 0.004 Msun, with a distance
of 525 +- 40 pc and a transverse velocity of 113 +- 21 km/s. The velocity
places the lens in the thick disk, making this the lowest-mass thick-disk brown
dwarf detected so far. Follow-up observations may allow one to observe the
light from the brown dwarf itself, thus serving as an important constraint for
evolutionary models of these objects and potentially opening a new window on
sub-stellar objects. The low a priori probability of detecting a thick-disk
brown dwarf in this event, when combined with additional evidence from other
observations, suggests that old substellar objects may be more common than
previously assumed.Comment: ApJ Letters, in press, 15 pages including 2 figure
Addressing Cervical Cancer Disparities in Texas: Expansion of a Community-Based Prevention initiative For Medically Underserved Populations
Although cervical cancer is preventable, significant disparities exist in access to screening and prevention services. In medically underserved areas (MUAs) of Texas, these rates are 55% higher compared to the remainder of the US. In 2019, we expanded a multicomponent, comprehensive program to improve cervical cancer prevention in partnership with 13 clinics and mobile vans in MUAs of Texas. Our multicomponent intervention program consists of community education and patient navigation coupled with a training/mentoring program for local medical providers to perform diagnostic procedures and treatment for patients with abnormal screening results. Hands-on training courses to learn these skills are coupled with biweekly telementoring conferences using Project ECHO® (Extension for Community Healthcare Outcomes). This program was implemented in 2015 and expanded to other MUAs in Texas in 2019. From March 2019 to August 2022, 75,842 individuals were educated about cervical cancer screening and HPV vaccination. A total of 44,781 women underwent screening for cervical cancer, and 2,216 underwent colposcopy and 264 underwent LEEP. High-grade cervical dysplasia was diagnosed in 658 individuals and invasive cervical cancer in 33 individuals. We trained 22 providers to perform colposcopy and/or LEEP. In addition, 78 Project ECHO telementoring sessions were held with an average of 42 attendees per session, with 72 individual patient cases discussed. Our comprehensive community-based prevention initiative for medically underserved populations has led to a significant number of individuals undergoing cervical cancer screening in MUAs, as well as improved access to colposcopy and LEEP services
MOA-2010-BLG-477Lb: constraining the mass of a microlensing planet from microlensing parallax, orbital motion and detection of blended light
Microlensing detections of cool planets are important for the construction of
an unbiased sample to estimate the frequency of planets beyond the snow line,
which is where giant planets are thought to form according to the core
accretion theory of planet formation. In this paper, we report the discovery of
a giant planet detected from the analysis of the light curve of a
high-magnification microlensing event MOA-2010-BLG-477. The measured
planet-star mass ratio is and the projected
separation is in units of the Einstein radius. The angular
Einstein radius is unusually large mas. Combining
this measurement with constraints on the "microlens parallax" and the lens
flux, we can only limit the host mass to the range . In
this particular case, the strong degeneracy between microlensing parallax and
planet orbital motion prevents us from measuring more accurate host and planet
masses. However, we find that adding Bayesian priors from two effects (Galactic
model and Keplerian orbit) each independently favors the upper end of this mass
range, yielding star and planet masses of
and at a distance of kpc,
and with a semi-major axis of AU. Finally, we show that the
lens mass can be determined from future high-resolution near-IR adaptive optics
observations independently from two effects, photometric and astrometric.Comment: 3 Tables, 12 Figures, accepted in Ap
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