To focus-match or not to focus-match inverse spatially offset Raman spectroscopy : a question of light penetration

Funding: The work was supported by funding from the UK Engineering and Physical Sciences Research Council (EP/P030017/1 and EP/R004854/1) and the H2020 FETOPEN project “Dynamic” (EC-GA 863203).The ability to identify the contents of a sealed container, without the need to extract a sample, is desirable in applications ranging from forensics to product quality control. One technique suited to this is inverse spatially offset Raman spectroscopy (ISORS) which illuminates a sample of interest with an annular beam of light and collects Raman scattering from the centre of the ring, thereby retrieving the chemical signature of the contents while suppressing signal from the container. Here we explore in detail the relative benefits of a recently developed variant of ISORS, called focus-matched ISORS. In this variant, the Fourier relationship between the annular beam and a tightly focused Bessel beam is exploited to focus the excitation light inside the sample and to match the focal point of excitation and collection optics to increase the signal from the contents without out compromising the suppression of the container signal. Using a flexible experimental setup which can realise both traditional and focus-matched ISORS, and Monte-Carlo simulations, we elucidate the relative advantages of the two techniques for a range of optical properties of sample and container.Publisher PDFPeer reviewe

Monitoring Soil Erosion on a Burned Site in the Mojave-Great Basin Transition Zone: Final Report for the Jacob Fire Site

A historic return interval of 100 years for large fires in the U.S. southwestern deserts is being replaced by one where fires may reoccur as frequently as every 20 to 30 years. The shortened return interval, which translates to an increase in fires, has implications for management of Soil Corrective Action Units (CAUs) and Corrective Action Sites (CASs) for which the Department of Energy, National Nuclear Security Administration Nevada Field Office has responsibility. A series of studies was initiated at uncontaminated analog sites to better understand the possible impacts of erosion and transport by wind and water should contaminated soil sites burn. The first of these studies was undertaken at the Jacob Fire site approximately 12 kilometers (7.5 miles) north of Hiko, Nevada. A lightning-caused fire burned approximately 200 hectares during August 6-8, 2008. The site is representative of a transition between Mojave and Great Basin desert ecoregions on the Nevada National Security Site (NNSS), where the largest number of Soil CAUs/CASs are located. The area that burned at the Jacob Fire site was primarily a Coleogyne ramosissima (blackbrush) and Ephedra nevadensis (Mormon tea) community, also an abundant shrub assemblage in the similar transition zone on the NNSS. This report summarizes three years of measurements after the fire. Seven measurement campaigns at the Jacob Fire site were completed. Measurements were made on burned ridge (upland) and drainage sites, and on burned and unburned sites beneath and between vegetation. A Portable In-Situ Wind Erosion Lab (PI-SWERL) was used to estimate emissions of suspended particles at different wind speeds. Context for these measurements was provided through a meteorological tower that was installed at the Jacob Fire site to obtain local, relevant environmental parameters. Filter samples, collected from the exhaust of the PI-SWERL during measurements, were analyzed for chemical composition. Runoff and water erosion were quantified through a series of rainfall/runoff simulation tests in which controlled amounts of water were delivered to the soil surface in a specified amount of time. Runoff data were collected from understory and interspace soils on burned ridge and drainage areas. Runoff volume and suspended sediment in the runoff were sampled; the particle size distribution of the sediment was determined by laboratory analysis. Several land surface and soil characteristics associated with runoff were integrated by the calculation of site-specific curve numbers. Several vegetation surveys were conducted to assess post-burn recovery. Data from plots in both burned and unburned areas included species identification, counts, and location. Characterization of fire-affected area included measures at both the landscape scale and at specific sites. Although wind erosion measurements indicate that there are seasonal influences on almost all parameters measured, several trends were observed. PI-SWERL measurements indicated the potential for PM10 windblown dust emissions was higher on areas that were burned compared to areas that were not. Among the burned areas, understory soils in drainage areas were the most emissive, and interspace soils along burned ridges were least emissive. By 34 months after the burn (MAB), at the end of the study, emissions from all burned soil sites were virtually indistinguishable from unburned levels. Like the amount of emissions, the chemical signature of the fire (indicated by the EC-Soil ratio) was elevated immediately after the fire and approached pre-burn levels by 24 MAB. Thus, the potential for wind erosion at the Jacob Fire site, as measured by the amount and type of emissions, increased significantly after the fire and returned to unburned levels by 24 MAB. The effect of fire on the potential for water erosion at the Jacob Fire site was more ambiguous. Runoff and sediment from ridge interspace soils and unburned interspace soils were similar throughout the study period. Seldom, if ever, did runoff and sediment occur in burned drainage area soils. For burned soils where runoff occurred at 1 MAB, the sediment size was finer than on unburned sites, but this effect disappeared by 3 MAB. For the three year study under the conditions tested at the Jacob Fire site, the potential for water erosion appeared relatively unaffected by the fire. Vegetation responses were documented for each year following the fire. By the end of the study, there was a substantial difference in plant densities and richness between drainage and ridge sites. Cheatgrass densities were higher in unburned plots, and cheatgrass was also more dominant in the community composition in unburned plots. Cheatgrass had increased in the burned area but so did other native species. Three years after the fire, the burned landscape continued to revegetate but had yet to approximate the condition of an unburned landscape. The results from the vegetation surveys support the wind erosion results, where the primary source of windborne particles originate from the understory, where lower plant diversity and densities were found. The soil appears to be more resilient and have a much shorter recovery time than the vegetation in this particular community

Autonomous quantum error correction of Gottesman-Kitaev-Preskill states

The Gottesman-Kitaev-Preskill (GKP) code encodes a logical qubit into a bosonic system with resilience against single-photon loss, the predominant error in most bosonic systems. Here we present experimental results demonstrating quantum error correction of GKP states based on reservoir engineering of a superconducting device. Error correction is made autonomous through an unconditional reset of an auxiliary transmon qubit. The lifetime of the logical qubit is shown to be increased from quantum error correction, therefore reaching the point at which more errors are corrected than generated.Comment: 6 pages, 3 figures + 26 pages, 12 figure

Insights into metazoan evolution from <i>Alvinella pompejana</i> cDNAs

BackgroundAlvinella pompejana is a representative of Annelids, a key phylum for evo-devo studies that is still poorly studied at the sequence level. A. pompejana inhabits deep-sea hydrothermal vents and is currently known as one of the most thermotolerant Eukaryotes in marine environments, withstanding the largest known chemical and thermal ranges (from 5 to 105°C). This tube-dwelling worm forms dense colonies on the surface of hydrothermal chimneys and can withstand long periods of hypo/anoxia and long phases of exposure to hydrogen sulphides. A. pompejana specifically inhabits chimney walls of hydrothermal vents on the East Pacific Rise. To survive, Alvinella has developed numerous adaptations at the physiological and molecular levels, such as an increase in the thermostability of proteins and protein complexes. It represents an outstanding model organism for studying adaptation to harsh physicochemical conditions and for isolating stable macromolecules resistant to high temperatures.ResultsWe have constructed four full length enriched cDNA libraries to investigate the biology and evolution of this intriguing animal. Analysis of more than 75,000 high quality reads led to the identification of 15,858 transcripts and 9,221 putative protein sequences. Our annotation reveals a good coverage of most animal pathways and networks with a prevalence of transcripts involved in oxidative stress resistance, detoxification, anti-bacterial defence, and heat shock protection. Alvinella proteins seem to show a slow evolutionary rate and a higher similarity with proteins from Vertebrates compared to proteins from Arthropods or Nematodes. Their composition shows enrichment in positively charged amino acids that might contribute to their thermostability. The gene content of Alvinella reveals that an important pool of genes previously considered to be specific to Deuterostomes were in fact already present in the last common ancestor of the Bilaterian animals, but have been secondarily lost in model invertebrates. This pool is enriched in glycoproteins that play a key role in intercellular communication, hormonal regulation and immunity.ConclusionsOur study starts to unravel the gene content and sequence evolution of a deep-sea annelid, revealing key features in eukaryote adaptation to extreme environmental conditions and highlighting the proximity of Annelids and Vertebrates

New technologies for 3D realization in Art and Design practice

As digital design technologies become ever more widespread, CAD-CAM, virtual and rapid prototyping techniques are increasingly being exploited by creative practitioners working in areas outside the industrial design and engineering contexts in which these technologies are currently predominantly employed. This review paper aims to critically examine work by artists, craft practitioners, and designer-makers who creatively engage with these new and rapidly emerging technologies and, by doing so, extend their own practice and push at the boundaries of art and design disciplines. Historic precedents for new 3D technologies in the fine and applied arts are identified, and writing by Heidegger, Baudrillard, and Virilio informs the critical review of work by art and design practitioners in sculpture, metalwork, jewellery, and ceramics. The discussion reflects on relationships between art and technology and physical and virtual making, and concludes by pointing to the possibility of new “hybrid” forms of practice which bridge the gap between physical and virtual design worlds. The paper closes by suggesting that the notion of “truth to materials” in the arts and crafts might now be extended to one of “truth to virtual materials”, as practitioners creatively negotiate relationships between digital cause and physical effect

Market segmentation strategies for complex automotive products

With the advent of 'big data', the purpose of this empirical study was to take the opportunity to rethink conventional market segmentation strategies. This is particularly relevant for the automotive industry which is going through a period of rapid change with advanced technologies such as electric powered and autonomous vehicles, creating increased concerns as to how this complexity is communicated effectively. A mixed methods approach was utilised to collect data from multiple sources, incorporating in-depth discussion groups, semi-structured interviews, an online survey, and data collection of communication processes through the attendance of new car product launches. The results suggest that marketing departments should rethink their data capture methods to collect more relevant consumer information, not the contemporary trend of needs, attitude, and motivation variables that are difficult to identify and collect, but basic information on their level of familiarity with products through previous experience and exposure. The basic dimensions identified are characterised by a consumer's expertise, involvement, and familiarity with a product. The findings are synthesised into a theoretical framework to define differing levels of product complexity, which would enable manufacturers to provide more closely defined market segmentation strategies when communicating new product information

The counselling self-estimate inventory (COSE): Does it work in Chinese counsellors?

Counselling self-efficacy is an important construct for research and evaluation in counsellors' competencies and training effectiveness. Larson et al. developed the Counselling Self-Estimate Inventory (COSE) for counsellors in America and examined its factor structure using exploratory factor analysis. They recommended a five-factor model (microskills, counselling process, difficult client behaviour, cultural competence, and awareness of values) and the use of the COSE for future research. However, little research has investigated the validity of the COSE in the context of counselling Chinese students in schools. In the present study, the factor structure of responses to the Chinese version of the Counselling Self-Estimate Inventory in a sample of 578 Hong Kong secondary school guidance teachers was examined using the EQS approach to confirmatory factor analysis. The results showed that while a five-factor model was fairly able to fit the data, the deletion of items related to the awareness of values factor yielded a better fitting model. The discussion of potential uses and limitations of the C-COSE in the context of preparing and supervising school guidance personnel in student counselling is relevant to counselling psychologists and researchers in Hong Kong and other parts of the world.postprin

Tomato: a crop species amenable to improvement by cellular and molecular methods

Tomato is a crop plant with a relatively small DNA content per haploid genome and a well developed genetics. Plant regeneration from explants and protoplasts is feasable which led to the development of efficient transformation procedures. In view of the current data, the isolation of useful mutants at the cellular level probably will be of limited value in the genetic improvement of tomato. Protoplast fusion may lead to novel combinations of organelle and nuclear DNA (cybrids), whereas this technique also provides a means of introducing genetic information from alien species into tomato. Important developments have come from molecular approaches. Following the construction of an RFLP map, these RFLP markers can be used in tomato to tag quantitative traits bred in from related species. Both RFLP's and transposons are in the process of being used to clone desired genes for which no gene products are known. Cloned genes can be introduced and potentially improve specific properties of tomato especially those controlled by single genes. Recent results suggest that, in principle, phenotypic mutants can be created for cloned and characterized genes and will prove their value in further improving the cultivated tomato.