Разработка технологии изготовления центратора

Целью выпускной квалификационной работы является: разработка технологического процесса изготовления центратора. В результате работы был произведен технологический анализ детали, составлен маршрут обработки. Разработан технологический процесс изготовления детали - центратор, произведён размерный анализ технологического процесса. Рассчитаны режимы и силы резания обработки. Подобрано оборудование, необходимое для изготовления центратора, и инструмент, которым будет производится обработка. Также спроектирована технологическая оснастка, необходимая для базирования детали на станках.As a result of the work was made а technological analysis of the detail. A route for machining parts has been developed. The technological process of manufacturing the centralizer part has been developed. Calculated cutting parameters and cutting forces in the process of machining parts. The equipment necessary for manufacturing the centralizer and the tool that will be used for processing have been selected. The technological equipment necessary for basing the part on machines is also designed. An operating map of the technological process has been compiled

A Recessive Mutation Resulting in a Disabling Amino Acid Substitution (T194R) in the LHX3 Homeodomain Causes Combined Pituitary Hormone Deficiency

Background/Aims: Recessive mutations in the LHX3 homeodomain transcription factor gene are associated with developmental disorders affecting the pituitary and nervous system. We describe pediatric patients with combined pituitary hormone deficiency (CPHD) who harbor a novel mutation in LHX3. Methods: Two female siblings from related parents were examined. Both patients had neonatal complications. The index patient had CPHD featuring deficiencies of GH, LH, FSH, PRL, and TSH, with later onset of ACTH deficiency. She also had a hypoplastic anterior pituitary, respiratory distress, hearing impairment, and limited neck rotation. The LHX3 gene was sequenced and the biochemical properties of the predicted altered proteins were characterized. Results: A novel homozygous mutation predicted to change amino acid 194 from threonine to arginine (T194R) was detected in both patients. This amino acid is conserved in the DNA-binding homeodomain. Computer modeling predicted that the T194R change would alter the homeodomain structure. The T194R protein did not bind tested LHX3 DNA recognition sites and did not activate the a-glycoprotein and PRL target genes. Conclusion: The T194R mutation affects a critical residue in the LHX3 protein. This study extends our understanding of the phenotypic features, molecular mechanism, and developmental course associated with mutations in the LHX3 gene. copyright (C) 2012 S. Karger AG, Base

Внедрение корпоративной системы управления проектами для реализации стратегических проектов компании

В целях реализации Восточной газовой программы ООО "Газпром трансгаз Томск" разработало Стратегию развития до 2020 г. и План организационно-технических мероприятий. Для успешного выполнения перспективных задач в организации формируется новая, современная система управления - корпоративная система управления проектами. Разработана методика внедрения корпоративной системы управления проектами, состав системы, определены ключевые участники и критические факторы успеха, выполнен расчет экономической эффективности проекта

Исследование парогазовой установки, работающей на генераторном газе

Выпускная квалификационная работа 116 страниц, 25 рисунков, 25 таблиц, 35 источников, 2 приложения. Ключевые слова: газификация угля, парогазовая установка, газотурбинная установка, генераторный газ, анализ, котел-утилизатор, показатели эффективности. Объектом исследования является ПГУ, работающая на генераторном газе. Цель работы – анализ работоспособности такой ПГУ с ГТУ, работающей на генераторном газе. В процессе исследования проводилось изучение различных способов газификации угля; производился выбор расчетной схемы ПГУ с газификацией; проводился расчет состава и характеристик генераторного газа и определение основных показателей тепловой эффективности ПГУ. В результате исследования был сделан анализ эффективности работы парогазовой установки.Final qualifying work of 116 pages, 25 figures, 25 tables, 35 sources, 2 annexes. Keywords: coal gasification combined cycle plant, a gas turbine plant, the product gas analysis, waste heat boiler, performance indicators. The object of this study is to CCGT running on syngas. The purpose of work - performance analysis of a CCGT with a gas turbine, operating on syngas. The study was carried out to study different methods of coal gasification; selects the estimated PSU circuit with gasification; conducted calculation and composition of the product gas characteristics and the definition of the main indicators of the thermal efficiency of the CCGT. The survey was made the analysis of the efficiency of the combined-cycle plant

The cyclin-dependent kinase inhibitor p57(Kip2) is epigenetically regulated in carboplatin resistance and results in collateral sensitivity to the CDK inhibitor seliciclib in ovarian cancer

Carboplatin remains a first-line agent in the management of epithelial ovarian cancer (EOC). Unfortunately, platinum-resistant disease ultimately occurs in most patients. Using a novel EOC cell line with acquired resistance to carboplatin: PEO1CarbR, genome-wide micro-array profiling identified the cyclin-dependent kinase inhibitor p57(Kip2) as specifically downregulated in carboplatin resistance. Presently, we describe confirmation of these preliminary data with a variety of approaches

Matrix metalloproteinase-9 is up-regulated by CCL21/CCR7 interaction via extracellular signal-regulated kinase-1/2 signaling and is involved in CCL21-driven B-cell chronic lymphocytic leukemia cell invasion and migration

4 páginas, 2 figuras -- PAGS nros. 383-386B-cell chronic lymphocytic leukemia (B-CLL) progression is frequently accompanied by clinical lymphadenopathy, and the CCL21 chemokine may play an important role in this process. Indeed, CCR7 (the CCL21 receptor), as well as matrix metalloproteinase-9 (MMP-9), are overexpressed in infiltrating B-CLL cells. We have studied whether MMP-9 is regulated by CCL21 and participates in CCL21-dependent migration. CCL21 significantly increased B-CLL MMP-9 production, measured by gelatin zymography. This was inhibited by blocking extracellular signal-regulated kinase-1/2 (ERK1/2) activity or by cell transfection with CCR7-siRNA. Accordingly, CCL21/CCR7 interaction activated the ERK1/2/c-Fos pathway and increased MMP-9 mRNA. CCL21-driven B-CLL cell migration through Matrigel or human umbilical vein endothelial cells (HUVEC) was blocked by anti-CCR7 antibodies, CCR7-siRNA transfection, or the ERK1/2 inhibitor U0126, as well as by anti-MMP-9 antibodies or tissue inhibitor of metalloproteinase 1 (TIMP-1). These results strongly suggest that MMP-9 is involved in B-CLL nodal infiltration and expand the roles of MMP-9 and CCR7 in B-CLL progression. Both molecules could thus constitute therapeutic targets for this diseaseThis work was supported by grants PI060400 (to A.G.P.) and PI061637 (to M.J.T.) from the Ministerio de Sanidad y Consumo, and by the Fundación de Investigación Médica Mutua Madrileña (FMM; to A.G.P.). J.R.M. was supported by FMM and the Fundación Ramón ArecesPeer reviewe

Activity and expression of urokinase-type plasminogen activator and matrix metalloproteinases in human colorectal cancer

BACKGROUND: Matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9), and urokinase-type plasminogen activator (uPA) are involved in colorectal cancer invasion and metastasis. There is still debate whether the activity of MMP-2 and MMP-9 differs between tumors located in the colon and rectum. We designed this study to determine any differences in the expression of MMP-2, MMP-9 and uPA system between colon and rectal cancer tissues. METHODS: Cancer tissue samples were obtained from colon carcinoma (n = 12) and rectal carcinomas (n = 10). MMP-2 and MMP-9 levels were examined using gelatin zymography and Western blotting; their endogenous inhibitors, tissue inhibitor of metalloproteinase-2 (TIMP-2) and tissue inhibitor of metalloproteinase-1 (TIMP-1), were assessed by Western blotting. uPA, uPAR and PAI-1 were examined using enzyme-linked immunosorbent assay (ELISA). The activity of uPA was assessed by casein-plasminogen zymography. RESULTS: In both colon and rectal tumors, MMP-2, MMP-9 and TIMP-1 protein levels were higher than in corresponding paired normal mucosa, while TIMP-2 level in tumors was significantly lower than in normal mucosa. The enzyme activities or protein levels of MMP-2, MMP-9 and their endogenous inhibitors did not reach a statistically significant difference between colon and rectal cancer compared with their normal mucosa. In rectal tumors, there was an increased activity of uPA compared with the activity in colon tumors (P = 0.0266), however urokinase-type plasminogen activator receptor (uPAR) and plasminogen activator inhibitor-1 (PAI-1) showed no significant difference between colon and rectal cancer tissues. CONCLUSION: These findings suggest that uPA may be expressed differentially in colon and rectal cancers, however, the activities or protein levels of MMP-2, MMP-9, TIMP-1, TIMP-2, PAI-1 and uPAR are not affected by tumor location in the colon or the rectum