Towards Optimal Energy-Water Supply System Operation for Agricultural and Metropolitan Ecosystems

The energy-water demands of metropolitan regions and agricultural ecosystems are ever-increasing. To tackle this challenge efficiently and sustainably, the interdependence of these interconnected resources has to be considered. In this work, we present a holistic decision-making framework which takes into account simultaneously a water and energy supply system with the capability of satisfying metropolitan and agricultural resource demands. The framework features: (i) a generic large-scale planning and scheduling optimization model to minimize the annualized cost of the design and operation of the energy-water supply system, (ii) a mixed-integer linear optimization formulation, which relies on the development of surrogate models based on feedforward artificial neural networks and first-order Taylor expansions, and (iii) constraints for land and water utilization enabling multi-objective optimization. The framework provides the operational profiles of all energy-water system elements over a given time horizon, which uncover potential synergies between the essential food, energy, and water resource supply systems.Comment: Part of the Foundations of Computer-Aided Process Operations and Chemical Process Control (FOCAPO/CPC) 2023 Proceeding

Human white adipose tissue vasculature contains endothelial colony-forming cells with robust in vivo vasculogenic potential

Epub ahead of print.-- The final publication is available at link.springer.comBlood-derived endothelial colony-forming cells (ECFCs) have robust vasculogenic potential that can be exploited to bioengineer long-lasting human vascular networks in vivo. However, circulating ECFCs are exceedingly rare in adult peripheral blood. Because the mechanism by which ECFCs are mobilized into circulation is currently unknown, the reliability of peripheral blood as a clinical source of ECFCs remains a concern. Thus, there is a need to find alternative sources of autologous ECFCs. Here we aimed to determine whether ECFCs reside in the vasculature of human white adipose tissue (WAT) and to evaluate if WAT-derived ECFCs (watECFCs) have equal clinical potential to blood-derived ECFCs. We isolated the complete endothelial cell (EC) population from intact biopsies of normal human subcutaneous WAT by enzymatic digestion and selection of CD31+ cells. Subsequently, we extensively compared WAT-derived EC phenotype and functionality to bonafide ECFCs derived from both umbilical cord blood and adult peripheral blood. We demonstrated that human WAT is indeed a dependable source of ECFCs with indistinguishable properties to adult peripheral blood ECFCs, including hierarchical clonogenic ability, large expansion potential, stable endothelial phenotype, and robust in vivo blood vessel-forming capacity. Considering the unreliability and low rate of occurrence of ECFCs in adult blood and that biopsies of WAT can be obtained with minimal intervention in an ambulatory setting, our results indicate WAT as a more practical alternative to obtain large amounts of readily available autologous ECFCs for future vascular cell therapies.This work was supported by a National Institutes of Health Grant (R00EB009096, J. M.-M).Peer reviewe