Decay of an isolated monopole into a Dirac monopole configuration

We study numerically the detailed structure and decay dynamics of isolated monopoles in conditions similar to those of their recent experimental discovery. We find that the core of a monopole in the polar phase of a spin-1 Bose-Einstein condensate contains a small half-quantum vortex ring. Well after the creation of the monopole, we observe a dynamical quantum phase transition that destroys the polar phase. Strikingly, the resulting ferromagnetic order parameter exhibits a Dirac monopole in its synthetic magnetic field.Comment: 6 pages, 5 figure

Inert states of spin-S systems

We present a simple but efficient geometrical method for determining the inert states of spin-S systems. It can be used if the system is described by a spin vector of a spin-S particle and its energy is invariant in spin rotations and phase changes. Our method is applicable to an arbitrary S and it is based on the representation of a pure spin state of a spin-S particle in terms of 2S points on the surface of a sphere. We use this method to find candidates for some of the ground states of spinor Bose-Einstein condensates.Comment: 4 pages, 2 figures, minor changes, references added, typos correcte

Floquet analysis of the modulated two-mode Bose-Hubbard model

We study the tunneling dynamics in a time-periodically modulated two-mode Bose-Hubbard model using Floquet theory. We consider situations where the system is in the self-trapping regime and either the tunneling amplitude, the interaction strength, or the energy difference between the modes is modulated. In the former two cases, the tunneling is enhanced in a wide range of modulation frequencies, while in the latter case the resonance is narrow. We explain this difference with the help of Floquet analysis. If the modulation amplitude is weak, the locations of the resonances can be found using the spectrum of the non-modulated Hamiltonian. Furthermore, we use Floquet analysis to explain the coherent destruction of tunneling (CDT) occurring in a large-amplitude modulated system. Finally, we present two ways to create a NOON state (a superposition of $N$ particles in mode 1 with zero particles in mode 2 and vice versa). One is based on a coherent oscillation caused by detuning from a partial CDT. The other makes use of an adiabatic variation of the modulation frequency. This results in a Landau-Zener type of transition between the ground state and a NOON-like state.Comment: 16 pages, 11 figures; published in Phys. Rev.

Explicit expressions for the topological defects of spinor Bose-Einstein condensates

In this paper we first derive a general method which enables one to create expressions for vortices and monopoles. By using this method we construct several order-parameters describing the vortices and monopoles of Bose-Einstein condensates with hyperfine spin F=1 and F=2. We concentrate on defects which are topologically stable in the absence of an external magnetic field. In particular we show that in a ferromagnetic condensate there can be a vortex which does not produce any superfluid flow. We also point out that the order-parameter space of the cyclic phase of F=2 condensate consists of two disconnected sets. Finally we examine the effect of an external magnetic field on the vortices of a ferromagnetic F=1 condensate and discuss the experimental preparation of a vortex in this system.Comment: 17 pages, partly rewritten to improve clarity, conclusions unchange

Application of CRISPR/Cas9 Tools for Genome Editing in the White-Rot Fungus Dichomitus squalens

Dichomitus squalens is an emerging reference species that can be used to investigate white-rot fungal plant biomass degradation, as it has flexible physiology to utilize different types of biomass as sources of carbon and energy. Recent comparative (post-) genomic studies on D. squalens resulted in an increasingly detailed knowledge of the genes and enzymes involved in the lignocellulose breakdown in this fungus and showed a complex transcriptional response in the presence of lignocellulose-derived compounds. To fully utilize this increasing amount of data, efficient and reliable genetic manipulation tools are needed, e.g., to characterize the function of certain proteins in vivo and facilitate the construction of strains with enhanced lignocellulolytic capabilities. However, precise genome alterations are often very difficult in wild-type basidiomycetes partially due to extremely low frequencies of homology directed recombination (HDR) and limited availability of selectable markers. To overcome these obstacles, we assessed various Cas9-single guide RNA (sgRNA) ribonucleoprotein (RNP) -based strategies for selectable homology and non-homologous end joining (NHEJ) -based gene editing in D. squalens. We also showed an induction of HDR-based genetic modifications by using single-stranded oligodeoxynucleotides (ssODNs) in a basidiomycete fungus for the first time. This paper provides directions for the application of targeted CRISPR/Cas9-based genome editing in D. squalens and other wild-type (basidiomycete) fungi

Application of CRISPR/Cas9 Tools for Genome Editing in the White-Rot Fungus Dichomitus squalens

Dichomitus squalens is an emerging reference species that can be used to investigate white-rot fungal plant biomass degradation, as it has flexible physiology to utilize different types of biomass as sources of carbon and energy. Recent comparative (post-) genomic studies on D. squalens resulted in an increasingly detailed knowledge of the genes and enzymes involved in the lignocellulose breakdown in this fungus and showed a complex transcriptional response in the presence of lignocellulose-derived compounds. To fully utilize this increasing amount of data, efficient and reliable genetic manipulation tools are needed, e.g., to characterize the function of certain proteins in vivo and facilitate the construction of strains with enhanced lignocellulolytic capabilities. However, precise genome alterations are often very difficult in wild-type basidiomycetes partially due to extremely low frequencies of homology directed recombination (HDR) and limited availability of selectable markers. To overcome these obstacles, we assessed various Cas9-single guide RNA (sgRNA) ribonucleoprotein (RNP) -based strategies for selectable homology and non-homologous end joining (NHEJ) -based gene editing in D. squalens. We also showed an induction of HDR-based genetic modifications by using single-stranded oligodeoxynucleotides (ssODNs) in a basidiomycete fungus for the first time. This paper provides directions for the application of targeted CRISPR/Cas9-based genome editing in D. squalens and other wild-type (basidiomycete) fungi

Enabling and promoting walking rehabilitation by paired associative stimulation after incomplete paraplegia : a case report

Introduction: Paired associative stimulation (PAS) is a combination of transcranial magnetic stimulation (TMS) and peripheral nerve stimulation (PNS) and induces plastic changes in the human corticospinal tract. We have previously shown that PAS consisting of TMS pulses given at 100% of stimulator output and high-frequency PNS is beneficial for motor rehabilitation of patients with a chronic incomplete spinal cord injury (SCI). The therapeutic possibilities of this PAS variant for walking rehabilitation of paraplegic patients are unexplored. Case presentation: A 47-year old man with traumatic incomplete paraplegia (AIS D, neurological level T7) received PAS to his left leg for 3 months at 12 months post injury (PAS1) and for an additional 3 months at 24 months post injury (PAS2). The right leg had normal AIS scores and was not stimulated. Before PAS, the patient was nonambulatory, could not stand without weight support, and was consequently not eligible for conventional walking rehabilitation. After PAS1, the patient could stand for 1.5 min and take 13 steps (24 steps in follow up) on parallel bars without weight support and was enrolled into conventional walking rehabilitation. He achieved independent walking ability with a rollator. During PAS2, walking distance increased 2.4 times faster than during the preceding year. The left leg AIS score and spinal cord independence measure mobility subscore increased. No adverse effects were detected. Discussion: This is the first report of PAS with a high-frequency peripheral component that enabled and promoted walking rehabilitation. Together with previous reports on this technique, this result encourages further research into its therapeutic potential and mechanism. © 2020, The Author(s).Peer reviewe