Vital dye labelling of Xenopus laevis trunk neural crest reveals multipotency and novel pathways of migration

Although the Xenopus embryo has served as an important model system for both molecular and cellular studies of vertebrate development, comparatively little is known about its neural crest. Here, we take advantage of the ease of manipulation and relative transparency of Xenopus laevis embryos to follow neural crest cell migration and differentiation in living embryos. We use two techniques to study the lineage and migratory patterns of frog neural crest cells: (1) injections of DiI or lysinated rhodamine dextran (LRD) into small populations of neural crest cells to follow movement and (2) injections of LRD into single cells to follow cell lineage. By using non-invasive approaches that allow observations in living embryos and control of the time and position of labelling, we have been able to expand upon the results of previous grafting experiments. Migration and differentiation of the labelled cells were observed over time in individual living embryos, and later in sections to determine precise position and morphology. Derivatives populated by the neural crest are the fins, pigment stripes, spinal ganglia, adrenal medulla, pronephric duct, enteric nuclei and the posterior portion of the dorsal aorta. In the rostral to mid-trunk levels, most neural crest cells migrate along two paths: a dorsal pathway into the fin, followed by presumptive fin cells, and a ventral pathway along the neural tube and notochord, followed by presumptive pigment, sensory ganglion, sympathetic ganglion and adrenal medullary cells. In the caudal trunk, two additional paths were noted. One group of cells moves circumferentially within the fin, in an arc from dorsal to ventral; another progresses ventrally to the anus and subsequently populates the ventral fin. By labelling individual precursor cells, we find that neural tube and neural crest cells often share a common precursor. The majority of clones contain labelled progeny cells in the dorsal fin. The remainder have progeny in multiple derivatives including spinal ganglion cells, pigment cells, enteric cells, fin cells and/or neural tube cells in all combinations, suggesting that many premigratory Xenopus neural crest precursors are multipotent

Segmental migration of trunk neural crest: time-lapse analysis reveals a role for PNA-binding molecules

Trunk neural crest cells migrate through the somites in a striking segmental fashion, entering the rostral but not caudal sclerotome, via cues intrinsic to the somites. Attempts to define the molecular bases of these cues have been hampered by the lack of an accessible assay system. To examine trunk neural crest migration over time and to perturb candidate guiding molecules, we have developed a novel explant preparation. Here, we demonstrate that trunk regions of the chicken embryo, placed in explant culture, continue to develop apparently normally for 2 days. Neural crest cells, recognized by prelabeling with DiI or by poststaining with the HNK-1 antibody, migrate in the somites of the explants in their typical segmental pattern. Furthermore, this paradigm allows us to follow trunk neural crest migration in situ for the first time using low-light-level videomicroscopy. The trajectories of individual neural crest cells were often complex, with cells migrating in an episodic mode encompassing forward, backward and lateral movements. Frequently, neural crest cells migrated in close-knit groups of 2–4 cells, moving at mean rates of migration of 10–14 µm/hour. Treatment of the explants with the lectin peanut agglutinin (PNA) both slowed the rate and altered the pattern of neural crest migration. Neural crest cells entered both the rostral and caudal halves of the sclerotome with mean rates of migration ranging from 6 to 13 µm/hour. These results suggest that peanut agglutinin-binding molecules are required for the segmental patterning of trunk neural crest migration. Because this approach permits neural crest migration to be both observed and perturbed, it offers the promise of more direct assays of the factors that influence neural crest development

Integrating Cellular and Molecular Approchaes into Studies of Development and Evolution: The Issue of Morphological Homology

Understanding the development and evolution of complex morphological characters requires broad, multidisciplinary approaches. By combining biological imaging, phylogenetic analyses, embryological manipulations and additional modem molecular techniques (e.g., whole mount in situ hybridization), we can address fundamental questions, such as the determination of homology. Many of the novel structures that evolved in vertebrates and distinguish them from their ancestors (such as jaws and the mechanosensory lateral line) are derived embryonically from two migratory ectodermal tissues, neural crest and/or various epidermal placodes. In particular, time-lapse cinematography of fluorescently labeled cells in living Xenopus and fish embryos has proved a powerful technique, revealing new information on migration and differentiation. These data allow us to reexamine the developmental criterion for morphological homology. There are three criteria for determining morphological homology of a structure between species: position (anatomical location), development (common origin, gene expression and/or cell behavior), and phylogeny (identification of the structure in an outgroup). Currently the phylogenetic criterion is the most regularly employed. Previous problems with the application of the developmental criterion were based on a paucity of detailed comparative developmental data and the overlooked assumption that ontogenies can not change during evolution and remain homologous while morphologies can. Homology has been defined as similarity due to continuity of information. The rejection of developmental data as a homology criterion is based on emphasizing lack of similarity without considering the information on continuity provided by these data. Often missing from previous analyses are the concepts of multiple developmental mechanisms in the formation of a structure (including such issues as developmental redundancies) and that ontogenies can change during evolution. By including these two concepts in a broadly comparative analysis, such problems as seemingly different developmental and genetic bases for homologous structures, can be explained and are even expected. As examples of how such an analysis can be done, we compared the development of the neural tube and lateral line among several vertebrate species. We show that while across species a given structure\u27s ontogeny may differ, a more broadly comparative developmental criterion using modern cell and molecular biological techniques provides a good homology criterion. Also, we find that molecular homology alone is not yet a good basis for morphological homology. While we would argue that a phylogenetic perspective is important (and critical for polarizing evolutionary changes), it is not essential for our usage of the developmental criterion. Thus, a broadly comparative and detailed understanding of developmental mechanisms makes for a robust criterion of homology

Algoritmo

Poema (texto en verso).Cátedra Libre Jacques Laca

Algoritmo

Poema (texto en verso).Cátedra Libre Jacques Laca

Aptitud anaerobia en deportistas de combate del sexo femenino

Se estudia en nivel de preparación anaerobia en tres selecciones femeninas de deportes de combate de alta calificación: 15 judocas, 7 esgrimistas de la modalidad sable y 16 taekwondoistas. Fueron medidos indicadores neuromusculares, al final de la preparación especial, utilizando los tests de ergosalto y de saltabilidad de Bosco, Se determinan las estadísticas descriptivas, significación de las diferencias entre deportes y la correlación entre indicadores. Se obtienen los resultados: Pala (15,7 ± 2,1-19,2 ± 1,5 W/Kg), Pana (14,5 ± 1,9-17,7 ± 1,2 W/Kg), Los SCAB, SSAB, SCPD, SCPCD y SDPC mas altos fueron los de las esgrimistas y la mejor coordinación se encontró en las competidoras de esgrima, mientras que los mayores valores de CELAS fueron los de las judocas. Los resultados obtenidos se corresponden con las características del gesto deportivo que prevalece en cada uno de los deportes estudiados

Analysis of sun glare on roundabouts with aerial laser scanning data

Road geometry and sun glares play an important role concerning road safety. In this research, the direct sunlight in a roundabout sited in Ávila (Spain) is analysed using Aerial Laser Scanning (ALS) point clouds. First, the roundabout is divided in 8 sections, obtaining the driver bearing vectors of the roundabout. Entrances and exits driver bearing vectors of the roundabout are also considered. Then, sun rays are generated for a specific location of the roundabout and in a specific day and time. The incidence of the sun rays with the driver’s vision angle is analysed based on human vision model. Finally, intersections of sun rays with obstacles are calculated utilizing ALS point clouds. ALS data is processed (removing outliers, reducing point density, and computing a Delaunay Triangulation) in order to obtain accurate intersection results with obstacles and optimise the computational time. The method was tested in a roundabout, considering different driver bearings, the slope of the road and the elevation of the terrain. The results show that sun glares are detected at any day and time of the year, therefore areas with risk of direct sun glare within the roundabout are identified. The sun ray’s incidence in the vision angle of the driver is higher during winter solstice, and intersections with obstacles occur mainly during sunrise and sunset. In roundabout vector 7, during winter solstice there is direct sun glare for 7 hours 30 minutes, at the equinoxes for 6 hours 15 minutes and during summer solstice there is no direct sun glare.Xunta de Galicia | Ref. ED481B-2019-061Xunta de Galicia | Ref. ED431C 2020/01Ministerio de Ciencia e Innovación | Ref. PID2019-105221RB-C43Ministerio de Ciencia e Innovación | Ref. TIN2016-77158 -C4-2-RMinisterio de Ciencia e Innovación | Ref. FJC2018-035550-

SEASONAL EVOLUTION AND SPATIAL DISTRIBUTION OF WEATHERING IN WESTERN GREENLAND

Through physical weathering, the Greenland Ice Sheet (GIS) produces sediments which are subsequently chemically weathered in three types of watersheds: 1) deglacial watersheds that are physically disconnected from the GIS and drain local precipitation, 2) proglacial watersheds that are hydrologically connected to the GIS, and 3) subglacial watersheds that form beneath the GIS. Chemical weathering in the glacial foreland may be important to atmospheric CO2 drawdown and oceanic fluxes of solutes, yet no holistic study exists that compares solute sources across all types of watersheds and through the melt season. Consequently, we investigated spatiotemporal changes in weathering through the 2013 ablation season from a transect of watersheds spanning the coast to the GIS in western Greenland. We sampled one proglacial (PG) watershed, from which we also assess subglacial (SG) weathering, one inland deglacial (IDG) and one coastal deglacial (CDG) watershed. A simple stoichiometric mass balance quantifies solute sources in each watershed. The principal solute source is trace carbonates in all watersheds; however, IDG has more carbonate (61 vs 36 mol%) and less silicate (3 vs 14 mol%) weathering than CDG. PG has similar carbonate (41 mol%) and silicate weathering (16 mol%) proportions to CDG, despite proximity to IDG. Weathering of biotite decreases from 12 mol% at PG to 3 mol% at CDG along an exposure age gradient, consistent with more radiogenic 87Sr/86Sr in waters at PG (0.73556) than DGC (0.71114). Carbonate weathering decreases and biotite + silicate weathering increases downstream through PG, reflecting increased weathering. Solute sources change little through time or space at IDG, but at PG, silicate weathering increases and carbonate weathering decreases as flow increases through the melt season, consistent with increased contributions of SG waters with long residence times in distributed channels. Thus, the evolution of SG through time and connections between subglacial reservoirs and main flow paths plays an important role in weathering at PG. As the GIS retreats, deglacial watersheds will constitute a greater fraction of the weathering flux and thus increased silicate weathering should alter solute fluxes to the oceans and increase atmospheric CO2 drawdown

Exposure age and climate controls on weathering in deglaciated watersheds of western Greenland

Fine-grained sediments deposited by retreating glaciers weather faster than the global average and this weathering can impact the global carbon cycle and oceanic fluxes of nutrients and radiogenic isotopes. Much work has focused on subglacial and proglacial weathering of continental ice sheets, but little is known about weathering and resulting fluxes from deglacial watersheds, which are disconnected from the ice sheets and discharge only annual precipitation and permafrost melt. We investigate the effects of exposure age and precipitation on weathering intensity in four deglacial watersheds on Greenland that form a transect from the coast near Sisimiut toward the Greenland Ice Sheet (GrIS) near Kangerlussuaq based on evaluations of major ion compositions, Sr isotope ratios, and mineral saturation states of waters and sediments. The transect is underlain by Archean orthogneiss and is characterized by gradients in moraine ages (∼7.5–8.0 ky inland to ∼10 ky at the coast) and water balance (−150 mm/yr inland to +150 mm/yr at the coast). Anion compositions are generally dominated by HCO3, but SO4 becomes increasingly important toward the coast, reflecting a switch from trace carbonate dissolution to sulfide mineral oxidation. Coastal watersheds have a higher proportion of dissolved silica, higher Na/Cl, Si/Ca, and lower Ca/Sr ratios than inland watersheds, indicating an increase in the relative proportion of silicate weathering and an increase in the extent of weathering toward the coast. More extensive weathering near the coast is also apparent in differences in the 87Sr/86Sr ratios of stream water and bedload (Δ87Sr/86Sr), which decreases from 0.017 inland to 0.005 at the coast, and in increased saturation states relative to amorphous SiO2 and quartz. The steep weathering gradient from inland to coastal watersheds reflects enhanced weathering compared to that expected from the 2 to 3 ky difference in exposure age caused by elevated coastal precipitation. The gradient of weathering with exposure age, water budget and distance from the ice sheet indicates that oceanic and atmospheric fluxes will change as continental glaciers retreat, precipitation patterns across the deglacial region readjust, and the relative proportion of deglacial to proglacial runoff increases