55 research outputs found
Didactic Conditions for the Implementation of Information and Digital Training of Masters of Vocational Training
ΠΠ°ΡΡΠΎΡΡΠ°Ρ ΡΡΠ°ΡΡΡ ΠΏΠΎΡΠ²ΡΡΠ΅Π½Π° ΠΏΡΠΎΠ±Π»Π΅ΠΌΠ°ΠΌ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΎΠ½Π½ΡΡ
ΠΊΠΎΠΌΠΏΠ΅ΡΠ΅Π½ΡΠΈΠΉ Ρ ΡΡΡΠ΄Π΅Π½ΡΠΎΠ² ΠΌΠ°Π³ΠΈΡΡΡΠ°ΡΡΡΡ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½ΠΈΡ ΠΡΠΎΡΠ΅ΡΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ΅ ΠΎΠ±ΡΡΠ΅Π½ΠΈΠ΅ (ΠΏΠΎ ΠΎΡΡΠ°ΡΠ»ΡΠΌ). ΠΡΡΠ²Π»Π΅Π½Ρ Π΄ΠΈΠ΄Π°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΡΠ»ΠΎΠ²ΠΈΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠΉ ΡΠ΅Π°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΡΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΠΌΠΎΠ΄Π΅Π»ΠΈ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΎΠ½Π½ΠΎ-ΡΠΈΡΡΠΎΠ²ΠΎΠΉ ΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠΈ ΠΌΠ°Π³ΠΈΡΡΡΠΎΠ² Π΄Π°Π½Π½ΠΎΠ³ΠΎ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½ΠΈΡ.This article is devoted to the problems of the formation of information competencies among students of the Master's degree in Vocational training. The didactic conditions for the effective implementation of the structural and functional model of information and digital training of masters in this field are revealed
Features of Assessing the Quality of Information-Digital Training of Teachers of Vocational Training
Π ΡΡΠ°ΡΡΠ΅ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡΡΡ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΠΏΠ΅Π΄Π°Π³ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΠ²Π°Π»ΠΈΠΌΠ΅ΡΡΠΈΠΈ Π΄Π»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΈΠ½ΡΠ΅Π³ΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΎΡΠ΅Π½ΠΊΠΈ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΎΠ½Π½ΠΎ-ΡΠΈΡΡΠΎΠ²ΠΎΠΉ ΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠΈ ΠΏΠ΅Π΄Π°Π³ΠΎΠ³ΠΎΠ² ΠΏΡΠΎΡΠ΅ΡΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΎΠ±ΡΡΠ΅Π½ΠΈΡ.The article is discussed the possibilities of using methods of pedagogical qualimetry to determine the integral assessment of the results of information and digital training of teachers of vocational training
Integral Assessment of the Skills of Future Teachers of Professional Training in the Field of Visualization of Engineering Processes
Π ΡΡΠ°ΡΡΠ΅ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°Π΅ΡΡΡ Π²Π°ΠΆΠ½ΠΎΡΡΡ ΠΏΡΠΎΡΠ΅Π΄ΡΡΡ ΠΎΡΡΡΠ΅ΡΡΠ²Π»Π΅Π½ΠΈΡ ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΠΎΠ±ΡΡΠ΅Π½ΠΈΡ ΠΈ Π²ΡΡΠ²Π»Π΅Π½ΠΈΡ ΠΊΡΠΈΡΠ΅ΡΠΈΠ΅Π² ΠΎΡΠ΅Π½ΠΊΠΈ ΡΠΌΠ΅Π½ΠΈΠΉ Π±ΡΠ΄ΡΡΠΈΡ
ΠΏΠ΅Π΄Π°Π³ΠΎΠ³ΠΎΠ² ΠΏΡΠΎΡΠ΅ΡΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΎΠ±ΡΡΠ΅Π½ΠΈΡ Π² ΠΎΠ±Π»Π°ΡΡΠΈ Π²ΠΈΠ·ΡΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠ½ΡΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ².The article is discussed the importance of the control procedure in the learning process and the identification of criteria for evaluating the skills of future teachers of vocational training in the field of visualization of engineering processes
The Use of Computer Animation Technology in the Study of Complex Engineering Disciplines
Π ΡΡΠ°ΡΡΠ΅ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°Π΅ΡΡΡ ΠΏΡΠΎΠ±Π»Π΅ΠΌΠ° ΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠΈ ΠΌΠΎΠ»ΠΎΠ΄Π΅ΠΆΠΈ ΠΊ ΡΠ°Π±ΠΎΡΠ΅ ΡΠΎ ΡΠ»ΠΎΠΆΠ½ΡΠΌΠΈ ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠ½ΡΠΌΠΈ ΡΠΈΡΡΠ΅ΠΌΠ°ΠΌΠΈ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ Π΅Π΅ ΡΠ΅ΡΠ΅Π½ΠΈΡ - Π²ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅ Π² ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΡΠΉ ΠΏΡΠΎΡΠ΅ΡΡ Π±ΡΠ΄ΡΡΠΈΡ
ΠΏΡΠ΅ΠΏΠΎΠ΄Π°Π²Π°ΡΠ΅Π»Π΅ΠΉ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΠΎΡΠ½ΠΎΠ²Π½ΡΡ
ΠΏΠΎΠ½ΡΡΠΈΠΉ, ΡΠ°ΠΊΡΠΎΠ² ΠΈ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π΄Π»Ρ Π²ΠΈΠ·ΡΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΈΠ½ΠΆΠ΅Π½Π΅ΡΠ½ΡΡ
ΡΠΈΡΡΠ΅ΠΌ, ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠΈ ΠΈ ΡΠΎΠΏΡΠΎΠ²ΠΎΠΆΠ΄Π΅Π½ΠΈΡ ΠΏΡΠΎΡΠ΅ΡΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎ-ΠΏΠ΅Π΄Π°Π³ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π΄Π΅ΡΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ.The article deals with the problem of preparing young people to work with complex engineering systems and methods of its solution - the inclusion in the educational process of future teachers, the use of basic concepts, facts and features of computer modeling technology for the visualization of engineering systems, the development and maintenance of professional and pedagogical activities
Quality Management of Information and Digital Training Students of Vocational Pedagogical University By Methods Pedagogical Qualimetry
Π ΡΡΠ°ΡΡΠ΅ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡΡΡ Π²ΠΎΠΏΡΠΎΡΡ ΠΎΡΠ΅Π½ΠΊΠΈ ΠΈ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΠΊΠ°ΡΠ΅ΡΡΠ²Π° ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΎΠ½Π½ΠΎ-ΡΠΈΡΡΠΎΠ²ΠΎΠΉ ΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠΈ Π±ΡΠ΄ΡΡΠΈΡ
ΠΏΠ΅Π΄Π°Π³ΠΎΠ³ΠΎΠ² ΠΏΡΠΎΡΠ΅ΡΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΎΠ±ΡΡΠ΅Π½ΠΈΡ Π² ΡΡΠ»ΠΎΠ²ΠΈΡΡ
ΡΠ΅Π°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΡΠ΅Π»Π΅ΠΉ ΡΠΈΡΡΠΎΠ²ΠΎΠΉ ΡΠΊΠΎΠ½ΠΎΠΌΠΈΠΊΠΈ. Π ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΠ³ΠΎ ΡΠ΅ΡΠ΅Π½ΠΈΡ ΠΏΡΠΎΠ±Π»Π΅ΠΌΡ ΠΏΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½ΠΎ Π²Π½Π΅Π΄ΡΠ΅Π½ΠΈΠ΅ Π² ΡΡΡΡΠΊΡΡΡΡ ΠΈ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΎΠ½Π½ΠΎ-ΡΠΈΡΡΠΎΠ²ΠΎΠΉ ΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΠΏΠ΅Π΄Π°Π³ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΠ²Π°Π»ΠΈΠΌΠ΅ΡΡΠΈΠΈ. ΠΠ΅ΡΠ°Π»ΡΠ½ΠΎ ΡΠ°ΡΡΠΌΠΎΡΡΠ΅Π½Ρ ΠΎΡΠ½ΠΎΠ²Π½ΡΠ΅ ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΡ ΠΈ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ ΠΊΠ²Π°Π»ΠΈΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΌΠΎΠ½ΠΈΡΠΎΡΠΈΠ½Π³Π° ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΎΠ½Π½ΠΎ-ΡΠΈΡΡΠΎΠ²ΠΎΠΉ ΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠΈ, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π° ΡΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½Π°Ρ ΠΌΠΎΠ΄Π΅Π»Ρ Π΅Π΅ ΠΎΡΡΡΠ΅ΡΡΠ²Π»Π΅Π½ΠΈΡ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΈΠ½ΡΠ΅Π³ΡΠ°ΡΠΈΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΠΏΠ΅Π΄Π°Π³ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΠ²Π°Π»ΠΈΠΌΠ΅ΡΡΠΈΠΈ.The article is dealed with the issues of assessing and improving the quality of information and digital training of future teachers of vocational training in the context of implementing the goals of the digital economy. As a possible solution to the problem, it is proposed to introduce the methods of pedagogical qualimetry into the structure and content of information-digital training. The main provisions and features of qualimetric monitoring of information-digital training are considered in detail, and a structural and functional model for its implementation is developed based on the integration of methods of pedagogical qualimetry
Π‘ΡΡΡΠΊΡΡΡΠ° ΠΈ ΠΎΠΏΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° ΠΏΠΎΠΊΡΡΡΠΈΠΉ Π°Π»ΠΌΠ°Π·ΠΎΠΏΠΎΠ΄ΠΎΠ±Π½ΠΎΠ³ΠΎ ΡΠ³Π»Π΅ΡΠΎΠ΄Π°
Using a hybrid method of cathodic arc (PVD) and chemical (CVD) deposition from the gas phase, a new type of coatings containing high amount of sp3 bonds of carbon, capable of absorbing effectively light has been developed.This thin film material is a promised one for optical devices operating in open space environment as antireflective coating Β for photoreceiver bodies. The hybrid method permits to obtain effective light absorbing coatings having excellent mechanical and tribological properties and is able to sustain temperature cycling in a range from plus 150 to minus 100 oC. Β The optical characteristics of DLC coatings were studied depending on the content of sp2bound content. The combined physical and chemical deposition of DLC coatings allows to achieve a sufficiently high light absorption (a~10 5 cmβ1) and low reflection with a relatively small coating thickness about 1 mm. It has been established that the antireflective properties of such coatings depend on the conditions for their preparation, first of all on hydrocarbon gas pressureΒ Π‘ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π³ΠΈΠ±ΡΠΈΠ΄Π½ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠΎΠ΄Π° ΠΊΠ°ΡΠΎΠ΄Π½ΠΎΒΠ΄ΡΠ³ΠΎΠ²ΠΎΠ³ΠΎ (PVD) ΠΈ Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ (CVD) ΠΎΡΠ°ΠΆΠ΄Π΅Π½ΠΈΡ ΠΈΠ· Π³Π°Π·ΠΎΠ²ΠΎΠΉ ΡΠ°Π·Ρ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½ Π½ΠΎΠ²ΡΠΉ ΡΠΈΠΏ ΠΏΠΎΠΊΡΡΡΠΈΠΉ Ρ Π±ΠΎΠ»ΡΡΠΈΠΌ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ΠΌ sp2ΒΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈ ΡΠ²ΡΠ·Π°Π½Π½ΡΡ
Π°ΡΠΎΠΌΠΎΠ² ΡΠ³Π»Π΅ΡΠΎΠ΄Π°, ΡΠΏΠΎΡΠΎΠ±Π½ΡΡ
ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎ ΠΏΠΎΠ³Π»ΠΎΡΠ°ΡΡ Π²ΠΈΠ΄ΠΈΠΌΠΎΠ΅ ΠΎΠΏΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΠ·Π»ΡΡΠ΅Π½ΠΈΠ΅.ΠΠΌΠΎΡΡΠ½ΡΠΉ ΡΠΎΠ½ΠΊΠΎΠΏΠ»Π΅Π½ΠΎΡΠ½ΡΠΉ ΡΠ³Π»Π΅ΡΠΎΠ΄Π½ΡΠΉ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π» ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌ Π΄Π»Ρ Π½Π°Π½Π΅ΡΠ΅Π½ΠΈΡ ΠΏΠΎΠ³Π»ΠΎΡΠ°ΡΡΠΈΡ
Π°Π½ΡΠΈΠ±Π»ΠΈΠΊΠΎΠ²ΡΡ
ΠΏΠΎΠΊΡΡΡΠΈΠΉ Π½Π° ΠΊΠΎΡΠΏΡΡΠ½ΡΠ΅ Π΄Π΅ΡΠ°Π»ΠΈ ΡΠΎΡΠΎΠΏΡΠΈΠ΅ΠΌΠ½ΠΈΠΊΠΎΠ² ΠΎΠΏΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΠ·Π»ΡΡΠ΅Π½ΠΈΡ, ΡΠ°Π±ΠΎΡΠ°ΡΡΠΈΡ
Π² ΠΎΡΠΊΡΡΡΠΎΠΌ ΠΊΠΎΡΠΌΠΎΡΠ΅. ΠΠΈΠ±ΡΠΈΠ΄Π½ΡΠΉ ΠΌΠ΅ΡΠΎΠ΄ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΏΠΎΠ»ΡΡΠ°ΡΡ ΡΠΈΠ»ΡΠ½ΠΎΠΏΠΎΠ³Π»ΠΎΡΠ°ΡΡΠΈΠ΅ ΠΏΠΎΠΊΡΡΡΠΈΡ, ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΠ΅ ΡΠ°ΠΊΠΆΠ΅ Π²ΡΡΠΎΠΊΠΈΠΌΠΈ ΠΌΠ΅Ρ
Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΈ ΡΡΠΈΠ±ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΡΠ²ΠΎΠΉΡΡΠ²Π°ΠΌΠΈ ΠΈ ΡΠΏΠΎΡΠΎΠ±Π½ΡΠ΅ Π²ΡΠ΄Π΅ΡΠΆΠΈΠ²Π°ΡΡ ΡΠ΅ΡΠΌΠΎΡΠΈΠΊΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΎΡ + 150 Π΄ΠΎ β 100 Β°Π‘.ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΎΠΏΡΠΈΡΠ΅ΡΠΊΠΈΡ
Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊ ΠΠΠ£ ΠΏΠΎΠΊΡΡΡΠΈΠΉ Π² Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΎΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ sp2ΒΡΠ²ΡΠ·Π°Π½Π½ΡΡ
Π°ΡΠΎΠΌΠΎΠ² ΡΠ³Π»Π΅ΡΠΎΠ΄Π°. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠΉ ΠΊΠΎΠΌΠ±ΠΈΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌ ΡΠΏΠΎΡΠΎΠ±ΠΎΠΌ ΡΠΈΠ·ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈ Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΎΡΠ°ΠΆΠ΄Π΅Π½ΠΈΡ ΠΠΠ£ Π² Π²ΠΈΠ΄Π΅ ΡΠΎΠ½ΠΊΠΈΡ
ΡΠ»ΠΎΠ΅Π² ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ Π΄ΠΎΡΡΠΈΡΡ Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎ Π²ΡΡΠΎΠΊΠΎΠ³ΠΎ ΠΏΠΎΠ³Π»ΠΎΡΠ΅Π½ΠΈΡ ΠΈ Π½ΠΈΠ·ΠΊΠΎΠ³ΠΎ ΠΎΡΡΠ°ΠΆΠ΅Π½ΠΈΡ ΠΏΡΠΈ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΠΎ ΠΌΠ°Π»ΠΎΠΉ ΡΠΎΠ»ΡΠΈΠ½Π΅ ΠΏΠΎΠΊΡΡΡΠΈΡ (a ~ 105 ΡΠΌβ1). Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π°Π½ΡΠΈΠ±Π»ΠΈΠΊΠΎΠ²ΡΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° ΡΠ°ΠΊΠΈΡ
ΠΏΠΎΠΊΡΡΡΠΈΠΉ Π·Π°Π²ΠΈΡΡΡ ΠΎΡ ΡΡΠ»ΠΎΠ²ΠΈΠΉ Π΅Π³ΠΎ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ, ΠΈ ΠΏΡΠ΅ΠΆΠ΄Π΅ Π²ΡΠ΅Π³ΠΎ β ΠΎΡ Π΄Π°Π²Π»Π΅Π½ΠΈΡ Π³Π°Π·Π° ΡΠ³Π»Π΅Π²ΠΎΠ΄ΠΎΡΠΎΠ΄ΠΎΠ²
Differential expression of HSPA1 and HSPA2 proteins in human tissues; tissue microarray-based immunohistochemical study
In the present study we determined the expression pattern of HSPA1 and HSPA2 proteins in various normal human tissues by tissue-microarray based immunohistochemical analysis. Both proteins belong to the HSPA (HSP70) family of heat shock proteins. The HSPA2 is encoded by the gene originally defined as testis-specific, while HSPA1 is encoded by the stress-inducible genes (HSPA1A and HSPA1B). Our study revealed that both proteins are expressed only in some tissues from the 24 ones examined. HSPA2 was detected in adrenal gland, bronchus, cerebellum, cerebrum, colon, esophagus, kidney, skin, small intestine, stomach and testis, but not in adipose tissue, bladder, breast, cardiac muscle, diaphragm, liver, lung, lymph node, pancreas, prostate, skeletal muscle, spleen, thyroid. Expression of HSPA1 was detected in adrenal gland, bladder, breast, bronchus, cardiac muscle, esophagus, kidney, prostate, skin, but not in other tissues examined. Moreover, HSPA2 and HSPA1 proteins were found to be expressed in a cell-type-specific manner. The most pronounced cell-type expression pattern was found for HSPA2 protein. In the case of stratified squamous epithelia of the skin and esophagus, as well as in ciliated pseudostratified columnar epithelium lining respiratory tract, the HSPA2 positive cells were located in the basal layer. In the colon, small intestine and bronchus epithelia HSPA2 was detected in goblet cells. In adrenal gland cortex HSPA2 expression was limited to cells of zona reticularis. The presented results clearly show that certain human tissues constitutively express varying levels of HSPA1 and HSPA2 proteins in a highly differentiated way. Thus, our study can help designing experimental models suitable for cell- and tissue-type-specific functional differences between HSPA2 and HSPA1 proteins in human tissues
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