Analysis on existent thermal insulating plasters towards innovative applications: evaluation methodology for a real cost-performance comparison

Thermal insulating plasters are an important means to face the energy efficiency issues in building field, above all in renovation processes. New solutions, such as nanotechnology or aerogel based plasters, could make a significant contribution to this field, reaching higher level of thermal performance and reducing needed thickness. But, in order to be really suitable for the market, new plaster solutions have to answer to specific economical and technical needs. This research provides an overall analysis of thermal insulating plasters in European market, comparing existing products according to technical specifications and economical features The main goal of this survey is to drive researches in thermal plasters fields towards innovative application, creating new plasters able to meets real market and end-users demands. Technical cross assessment considers three main factors, defined according to European standards: volume mass powder, dry bulk density of hardened mortar and thermal conductivity. Benchmarking analysis compares prices per unit, in order to relate material quantity to the achieving of common Rx value. Cross assessments results allow to define thermal, technical and economical requirements that new thermal insulating plasters have to meet to be suitable for European volume market

Risk factors for peri-operative high-grade complications in patients with prostate cancer treated with robotic radical prostatectomy

Aim of our study was to evaluate the risk factors for high-grade complications (Clavien Classification System ≥3a) in patients treated with with robotic assisted radical prostatectomy (RARP). Between 2012 and 2017, a consecutive series of patients with prostate cancer were treated with robotic radical prostatectomy (RRP). Demographic, clinical and histological characteristics of the patients were recorded. Histological specimens were graded according to the new PGG classification. Complications were classified according to the modified Clavien classification system (CCS). Logistic regression analysis was performed to evaluate the risk of high grade complications (CCS>3a)

Transcriptional Stress Induces Chromatin Relocation of the Nucleotide Excision Repair Factor XPG.

Endonuclease XPG participates in nucleotide excision repair (NER), in basal transcription, and in the processing of RNA/DNA hybrids (R-loops): the malfunction of these processes may cause genome instability. Here, we investigate the chromatin association of XPG during basal transcription and after transcriptional stress. The inhibition of RNA polymerase II with 5,6-dichloro-l-β-D-ribofuranosyl benzimidazole (DRB), or actinomycin D (AD), and of topoisomerase I with camptothecin (CPT) resulted in an increase in chromatin-bound XPG, with concomitant relocation by forming nuclear clusters. The cotranscriptional activators p300 and CREB-binding protein (CREBBP), endowed with lysine acetyl transferase (KAT) activity, interact with and acetylate XPG. Depletion of both KATs by RNA interference, or chemical inhibition with C646, significantly reduced XPG acetylation. However, the loss of KAT activity also resulted in increased chromatin association and the relocation of XPG, indicating that these processes were induced by transcriptional stress and not by reduced acetylation. Transcription inhibitors, including C646, triggered the R-loop formation and phosphorylation of histone H2AX (γ-H2AX). Proximity ligation assay (PLA) showed that XPG colocalized with R-loops, indicating the recruitment of the protein to these structures. These results suggest that transcriptional stress-induced XPG relocation may represent recruitment to sites of R-loop processing

Enhancement of Second Harmonic Signal in Nanofabricated Cones

Geometrical effects in optical nanostructures on nanoscale can lead to interesting phenomena such as inhibition of spontaneous emission,(1,2) high-reflecting omnidirectional mirrors, structures that exhibit low-loss-waveguiding,(3) and light confinement.(4,5) Here, we demonstrate a similar concept of exploiting the geometrical effects on nanoscale through precisely fabricating lithium niobate (LiNbO3) nanocones arrays devices. We show a strong second harmonic generation (SHG) enhancement, shape and arrangement dependent, up to 4 times bigger than the bulk one. These devices allow below diffraction limited observation, being perfect platforms for single molecule fluorescence microscopy(6) or single cell endoscopy.(7) Nanocones create a confined illumination volume, devoid from blinking and bleaching, which can excite molecules in nanocones proximity. Illumination volume can be increased by combining the SH enhancement effect with plasmon resonances, excited thanks to a gold plasmonic shell deposited around the nanostructures. This results in a local further enhancement of the SH signal up to 20 times. The global SH enhancement can be rationally designed and tuned through the means of simulations