Transcriptional activation of CBFβ by CDK11p110 is necessary to promote osteosarcoma cell proliferation.

BACKGROUND:Aberrant expression of cyclin-dependent protein kinases (CDK) is a hallmark of cancer. CDK11 plays a crucial role in cancer cell growth and proliferation. However, the molecular mechanisms of CDK11 and CDK11 transcriptionally regulated genes are largely unknown. METHODS:In this study, we performed a global transcriptional analysis using gene array technology to investigate the transcriptional role of CDK11 in osteosarcoma. The promoter luciferase assay, chromatin immunoprecipitation assay, and Gel Shift assay were used to identify direct transcriptional targets of CDK11. Clinical relevance and function of core-binding factor subunit beta (CBFβ) were further accessed in osteosarcoma. RESULTS:We identified a transcriptional role of protein-DNA interaction for CDK11p110, but not CDK11p58, in the regulation of CBFβ expression in osteosarcoma cells. The CBFβ promoter luciferase assay, chromatin immunoprecipitation assay, and Gel Shift assay confirmed that CBFβ is a direct transcriptional target of CDK11. High expression of CBFβ is associated with poor outcome in osteosarcoma patients. Expression of CBFβ contributes to the proliferation and metastatic behavior of osteosarcoma cells. CONCLUSIONS:These data establish CBFβ as a mediator of CDK11p110 dependent oncogenesis and suggest that targeting the CDK11- CBFβ pathway may be a promising therapeutic strategy for osteosarcoma treatment

Correction to: Transcriptional activation of CBFβ by CDK11p110 is necessary to promote osteosarcoma cell proliferation.

Following publication of the original article [1], it was reported that Figs. 4 and 5 were not updated during the production process

Purification and characterization of three laccase isozymes from the white rot fungus Trametes sp. HS-03

Three laccase isozymes (LacI, LacII and LacIII) were isolated from the culture supernatant solution of Trametes sp. HS-03. Diethylaminoethyl (DEAE)-sepharose fast flow anion exchange chromatography and Sephadex G-100 size-exclusion chromatography was performed to achieve electrophoretic homogeneity. The molecular masses (64.2, 60.7 and 38.9 kDa), isoelectric points [pIs (7.3, 4.7 and 3.5), and N-terminal amino acid sequences (G-I-G-P-V, A-I-G-P-T and S-I-G-P-V) were found to be different for the three laccase isozymes. LacI and II have similar thermostability, while LacIII showed better thermostability. LacIII also showed optimal activity at 80°C, with a half-life of 125 min at 70°C. The pI-value of LacI and the molecular mass of LacIII differ significantly from previously described fungal laccases.Keywords: Trametes sp. HS-03, laccase isozymes, purification, characterizatio

PyPop7: A Pure-Python Library for Population-Based Black-Box Optimization

In this paper, we present a pure-Python open-source library, called PyPop7, for black-box optimization (BBO). It provides a unified and modular interface for more than 60 versions and variants of different black-box optimization algorithms, particularly population-based optimizers, which can be classified into 12 popular families: Evolution Strategies (ES), Natural Evolution Strategies (NES), Estimation of Distribution Algorithms (EDA), Cross-Entropy Method (CEM), Differential Evolution (DE), Particle Swarm Optimizer (PSO), Cooperative Coevolution (CC), Simulated Annealing (SA), Genetic Algorithms (GA), Evolutionary Programming (EP), Pattern Search (PS), and Random Search (RS). It also provides many examples, interesting tutorials, and full-fledged API documentations. Through this new library, we expect to provide a well-designed platform for benchmarking of optimizers and promote their real-world applications, especially for large-scale BBO. Its source code and documentations are available at https://github.com/Evolutionary-Intelligence/pypop and https://pypop.readthedocs.io/en/latest, respectively.Comment: 5 page

Nanotube ferroelectric tunnel junctions with giant tunneling electroresistance ratio

Low-dimensional ferroelectric tunnel junctions are appealing for the realization of nanoscale nonvolatile memory devices due to their inherent advantage of device miniaturization. Those based on current mechanisms still have restrictions including low tunneling electroresistance (TER) effects and complex heterostructures. Here, we introduce an entirely new TER mechanism to construct the nanotube ferroelectric tunnel junction with ferroelectric nanotubes as the tunneling region. When rolling a ferroelectric monolayer into a nanotube, due to the coexistence of its intrinsic ferroelectric polarization with the flexoelectric polarization induced by bending, there occurs metal-insulator transition depending on radiative polarization states. For the pristine monolayer, its out-of-plane polarization is tunable by an in-plane electric field, the conducting states of the ferroelectric nanotube can thus be tuned between metallic and insulating via axial electric means. Using {\alpha}-In2Se3 as an example, our first-principles density functional theory calculations and nonequilibrium Green's function formalism confirm the feasibility of the TER mechanism and indicate an ultrahigh TER ratio exceeding 9.9*10^10% of the proposed nanotube ferroelectric tunnel junctions. Our findings provide a promising approach based on simple homogeneous structures for high density ferroelectric microelectronic devices with excellent ON/OFF performance.Comment: 15 pages, 5 figure

Approach to Higher Wheat Yield in the Huang-Huai Plain: Improving Post-anthesis Productivity to Increase Harvest Index

Both increased harvest index (HI) and increased dry matter (DM) are beneficial to yield; however, little is known about the priority of each under different yield levels. This paper aims to determine whether HI or DM is more important and identify the physiological attributes that act as indicators of increased yield. Two field experiments involving different cultivation patterns and water-nitrogen modes, respectively, were carried out from 2013 to 2016 in Huang-Huai Plain, China. Plant DM, leaf area index (LAI), and radiation interception (RI) were measured. Increased yield under low yield levels <7500 kg ha-1 was attributed to an increase in both total DM and HI, while increases under higher yield levels >7500 kg ha-1 were largely dependent on an increase in HI. Under high yield levels, HI showed a significant negative correlation with total DM and a parabolic relationship with net accumulation of DM during filling. Higher net accumulation of DM during filling helped slow down the decrease in HI, thereby maintaining a high value. Moreover, net DM accumulation during filling was positively correlated with yield, while post-anthesis accumulation showed a significant linear relationship with leaf area potential (LAP, R2 = 0.404–0.526) and radiation interception potential (RIP, R2 = 0.452–0.576) during grain filling. These findings suggest that the increase in LAP and RIP caused an increase in net DM accumulation after anthesis. Under DM levels >13,000 kg ha-1 at anthesis, maintaining higher LAI and RI in lower layers during grain formation contributed to higher yield. Furthermore, the ratio of upper- to lower-layer RI showed a second-order curve with yield during filling, with an increase in the optimal range with grain development. Pre-anthesis translocation amount, translocation ratios and contribution ratios also showed second-order curves under high yield levels, with optimal values of 3000–4500 kg ha-1, 25–35, and 30–50%, respectively. These results confirm the importance of HI in improving the yield, thereby providing a theoretical basis for wheat production in the Huang-Huai Plain

Genome-wide identification and expression analysis of the C2H2-zinc finger transcription factor gene family and screening of candidate genes involved in floral development in Coptis teeta Wall. (Ranunculaceae)

© 2023 The authors. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.3389/fgene.2024.1349673Background: C2H2-zinc finger transcription factors comprise one of the largest and most diverse gene superfamilies and are involved in the transcriptional regulation of flowering. Although a large number of C2H2 zinc-finger proteins (C2H2-ZFPs) have been well characterized in a number of model plant species, little is known about their expression and function in Coptis teeta. C. teeta displays two floral phenotypes (herkogamy phenotypes). It has been proposed that the C2H2-zinc finger transcription factor family may play a crucial role in the formation of floral development and herkogamy observed in C. teeta. As such, we performed a genome-wide analysis of the C2H2-ZFP gene family in C. teeta. Results: The complexity and diversity of C. teeta C2H2 zinc finger proteins were established by evaluation of their physicochemical properties, phylogenetic relationships, exon-intron structure, and conserved motifs. Chromosome localization showed that 95 members of the C2H2 zinc-finger genes were unevenly distributed across the nine chromosomes of C. teeta, and that these genes were replicated in tandem and segmentally and had undergone purifying selection. Analysis of cis-acting regulatory elements revealed a possible involvement of C2H2 zinc-finger proteins in the regulation of phytohormones. Transcriptome data was then used to compare the expression levels of these genes during the growth and development of the two floral phenotypes (F-type and M-type). These data demonstrate that in groups A and B, the expression levels of 23 genes were higher in F-type flowers, while 15 genes showed higher expressions in M-type flowers. qRT-PCR analysis further revealed that the relative expression was highly consistent with the transcriptome data. Conclusion: These data provide a solid basis for further in-depth studies of the C2H2 zinc finger transcription factor gene family in this species and provide preliminary information on which to base further research into the role of the C2H2 ZFPs gene family in floral development in C. teeta.This research was supported by the National Natural Science Foundation of China (project grant no: 31971543), Major Special Projects of Yunnan Province (Biomedicine) (project number: 202102AA310045), Yunnan Provincial Science and Technology Department Agriculture Joint Special Project (projectnumber:202101BD070001-008)