Functional characterization of Indian Ocean viral sequences from the viral and larger fractions of metagenomic data in the context of KEGG pathways.

<p>The inset pie charts represent the breakdown of the Metabolism super-pathway (top) and Energy metabolism pathway (bottom). The percentages of viral sequences attributed to the Energy metabolism pathway are indicated on the Metabolism pie charts.</p

Bar chart demonstrating the predicted taxonomy of bacterial host genera for assembled Indian Ocean viral metagenomic data.

<p>The area of each bar is proportional to the total number of viral reads contained in the contigs assigned to a particular host genus. Direct assignments are colored green and transitive assignments are colored blue. Direct assignments were made by assigning viral contigs to the host models built on NCBI RefSeq genomes. Transitive assignments were made by assigning viral contigs to models built on large bacterial contigs, and then assigning taxonomy to these bacterial contigs (as described in the text). Twenty top taxa in pairs of green and blue are shown according to the maximum count from either direct or transitive assignment method, respectively. If no bar is shown for one of the methods, the count was zero.</p

A phylogenetic tree built from amino acid sequences using FastTree for the gene encoding the large subunit terminase.

<p>Selected reference sequences are colored red and green. Representative Indian Ocean VF and LF sequences are colored pink and purple respectively. GOS Phase I sequences are colored blue. Confidence values are displayed on the tree.</p

Estimated diversity of Indian Ocean virioplankton assemblages using PHACCS.

<p>Frag: fragment; PE: paired end; Sub: random subsamples; SW: Shannon-Weiner.</p

Sanger and 454 pyrosequencing statistics for Indian Ocean viral metagenomes.

*<p>Sample not used in taxonomic and functional analyses due to cellular contamination. n/a denote where the data was not used in study. Percent of phylogenetic trees denotes the amount of sequences (ORFs) that were taxonomically classified via APIS.</p

Taxonomic characterization of Indian Ocean virioplankton assemblages.

a<p>% total protein sequences classified at highest (kingdom) taxonomic level per category (LF or VF).</p>b<p>% total protein sequences characterized as viral per category.</p>c<p>% of total of protein sequences characterized as dsDNA viruses comprising >0.1% in one category.</p>d<p>% of total protein sequences characterized as Caudoviruses per category.</p>e<p>% of total protein sequences characterized as NCLDVs per category.</p>f<p>% of total protein sequences characterized as Phycoviruses per category.</p><p>n/a = not available.</p

Principal component analysis (PCA) of the relative abundance of VF and LF viral sequences within protein clusters.

<p>Viral libraries are represented by the green circles and larger fraction libraries are represented by the blue triangles.</p