Efecto de la salinidad en la eficiencia en el uso del agua y la producción de materia seca en guayabo

En plantas de guayaba (Psidium guajava L. cvs. RS 980 y Cimpa 00916) se evaluó el efecto de la salinidad sobre el crecimiento, la producción de materia seca y la eficacia agronómica en el uso del agua. Las plantas se obtuvieron a partir de semilla, crecieron en un invernadero en materas (tres litros) y se expusieron a condiciones no salinas (ECe: 1.2 dS.m-1) o salinas mediante la adición de 20; 40; 60 y 80 mM de NaCl con base en suelo seco al aire (ECe:3.0, 5.4, 7.9 y 10.5 dS.m-1 respectivamente medidas en extracto de saturación) tres meses después de la germinación. Las plantas se cosecharon siete meses después de su exposición al NaCl. La tasa de evapotranspiración (cm3), la producción de la materia seca (g), la eï¬�cacia agronómica en el uso del agua (g.l-1) y el área de la hoja (cm2), en ambos cultivares se redujeron con la salinidad. La distribución de biomasa en raíces, tallos y hojas, así como la relación raíz: brote con base en materia seca resultaron poco afectadas por la salinidad. En comparación con las plantas control, la eï¬�ciencia agronómica en el uso del agua se redujo 29.5% en el cultivar RS 980 y 10.0% en Cimpa 00196 a 80 mM de NaCl, nivel que también disminuyó la producción total de materia seca 50.8% en RS 980 y 29.8% en Cimpa 00196. De este modo, el cultivar RS 980 parece más sensible a la salinidad que Cimpa 00196.Effect of salinity on water use efïciency and dry matter production of guava plants. To study the salt tolerance of guava cultivars (Psidium guajava I L.cvs RS 980 and Cimpa 00916), the effect os salinity on growth, dry matter production and agronomic water use eficiency were evaluated. Seedlings were grown in a glasshouse experiment in 3-1 post ï¬�lled with soil in Tunja, Colombia. Plants were exposed to either non-saline (ECe: 1.2 dS.m-1) or saline conditions using 20, 40, 60 and 80 mM NaCl based to air dry soil (ECe: 3.0, 5.4, 7.9 and 10.5 dS.m-1 respectly of a saturated soil extract) 3 months after germination. Plants were harvested 7 months after the NaCl exposure. The evapotranspiration rate (cm3), dry matter production (g), agronomic water use efï¬�ciency (g1-1) and leaf area (cm2) of both cultivars was reduced by salinity. Dry matter partitioning in roots shoot and leaves and root: shoot ratio based on dry matter were little affected by salinity. In comparison to the control plants, the agronomic water use efï¬�ciency was reduced 29.5% in cultivar RS 980 and 10.0% in Cimpa 00196 by 80 mM NaCl; similarly, 80 mM NaCl reduced the total dry matter production 50.8% in RS 980 and 29.8% in Cimpa 00196 in comparison to the control plants. Thus, it appears that RS 980 was more salt-sensitive than Cimpa 00196

How do university teachers use digital technologies in class?

[EN] The main goal of this article is the description of a peculiar textbook which aims to contribute in a friendly and available way to the teacher-student relationship in a subject devoted to the foundations of a discipline. Analytical Chemistry and Nanotechnology have been selected as case studies. The description of the true concept of innovation in the framework of Einstein and Khun thoughts, as well as the two approaches (top-down and bottom- up) to teach and learn the foundations of an area of knowledge is the subject matter of the Introduction of the paper. The so-named “textbook” is composed by two essential parts. On the one hand, a CD room contains 350 dynamic downloadable images (PowerPoint), and, on the other, a book containing the detailed explanation of each image and its dynamics, a glossary of terms and the detailed responses to 240 questions posed at the end of the chapters. The principal teaching-learning innovation consists of the major relative importance of the images versus the text, which is designed to support images. In this way, the roles of teachers and students are far from the traditional ones. Other innovations are as follows: the fact that an undergraduate student was a full co-author, the permanent cross references of the teaching message and the use of many examples from the real life. This innovation was successively extrapolated to an e-book devoted to the basic divulgation of nanotechnology to the citizens. This approach is subsequently described at the end of the article.[ES] En este artículo se describe una obra docente atípica, con intención de ser rompedora, orientada a contribuir de forma amigable y asequible a una innovación docente-discente de una materia universitaria dedicada a los fundamentos/principios de un área de conocimiento. Como “casos de estudio” se han utilizado la Química Analítica y la Nanotecnología. El contenido del artículo se contextualiza en el apartado Introducción del mismo donde se describe el concepto verdadero de innovación en el marco de los enfoques de Einstein y Khun, y se exponen las dos formas (top-down y bottom-up) de abordar la enseñanza-aprendizaje de los fundamentos de una área de conocimiento. La obra docente consta de un CD con aproximadamente 350 imágenes (descargables en PowerPoint ) con movimientos y un libro de texto que contiene la explicación detallada de cada diapositiva; así como dos anexos con un glosario de 250 términos y la resolución detallada de unas 240 cuestiones, respectivamente. La innovación docente-discente principal que aporta es que las imágenes tienen un mayor peso específico que el texto, lo que implica cambiar los roles del profesor y del estudiante en horas presenciales y no presenciales. Otras innovaciones a destacar son la participación de una estudiante como coautora, las referencias cruzadas permanentes para contextualizar al estudiante; así como el empleo permanente de ejemplos de la vida real y actual para apoyar los conceptos típicos de un libro de fundamentos. Esta innovación se ha extrapolado con éxito a un e-bookde divulgación de la nanotecnología a los ciudadanos, que también se describe sucintamente al final del artículo.Los autores desean mostrar su agradecimiento a la editorial de la Universidad de Córdoba, UCOPRESS, y especialmente a su director, Juan Pedro Monferrer, por la receptividad a estas dos propuestas atípicas (libro de texto y e-book), y su dedicación a superar los problemas que toda innovación (en este caso editorial) implica.González-Alfaya, M.; López-Jiménez, M.; López-Lorente, A.; Soriano Dotor, M.; Valcárcel, M. (2017). ¿Puede un libro de texto atípico inducir a la innovación docente-discente?. REDU. Revista de Docencia Universitaria. 15(2):295-314. doi:10.4995/redu.2017.7777SWORD29531415

First-line single-agent regorafenib in frail patients with metastatic colorectal cancer: a pilot phase II study of the Spanish Cooperative Group for the Treatment of Digestive Tumours (TTD)

BackgroundTreatment of frail patients with advanced colorectal cancer (CRC) is controversial. This pilot phase II trial aimed to assess the efficacy and safety of regorafenib when administered in first-line to frail patients with advanced CRC.MethodsFrail patients without prior advanced colorectal cancer treatment were included in the study. Definition of frailty was defined per protocol based on dependency criteria, presence of chronic comorbid pathologies and/or geriatric features. Main objective: to assess progression-free survival (PFS) rate at 6months. Treatment consisted of 28-daycycles of orally administered regorafenib 160mg/day (3 weeks followed by 1 week rest).ResultsForty-seven patients were included in the study. Median age was 81years (range 63-89). Frailty criteria: dependency was observed in 26 patients (55%), comorbidities in 27 (57%) and geriatric features in 18 (38%). PFS rate at 6months was 45% (95% confidence interval [CI] 30-60]. Median PFS was 5.6months (95%CI 2.7-8.4). Median overall survival (OS) was 16months (95%CI 7.8-24). Complete response, partial response and stable disease were observed in one, two and 21 patients respectively (objective response rate 6.4%; disease control rate 51%). Thirty-nine patients (83%) experienced grade 3-4 adverse events (AEs). The most common grade 3-4 AEs were hypertension (15 patients; 32%), asthenia (14; 30%), hypophosphatemia (6; 13%); diarrhea (4; 8%), hand-foot-skin reaction (4; 8%). There were two toxic deaths (4.2%) (grade 5 rectal bleeding and death not further specified). Dose reduction was required in 26 patients (55%) and dose-delays in 13 patients (28%).ConclusionsThe study did not meet the pre-specified boundary of 55% PFS rate at 6months. Toxicity observed (83% patients experienced grade 3 and 4 AEs) preclude its current use in clinical practice on this setting. Disease control rate and overall survival results are interesting and might warrant further investigation to identify those who benefit from this approach.Trial registrationThis trial was prospectively registered at EudraCT (2013-000236-94). Date of trial registration: April 9th, 2013

A synthetic peptide from transforming growth factor beta type III receptor inhibits liver fibrogenesis in rats with carbon tetrachloride liver injury

Transforming growth factor beta1 (TGF-beta1) is a pleiotropic cytokine, which displays potent profibrogenic effects and is highly expressed in fibrotic livers. For this reason, development of TGF-B1 inhibitors might be of great importance to control liver fibrogenesis as well as other undesired side effects due to this cytokine. Potential peptide inhibitors of TGF-beta1 (derived from TGF-beta1 and from its type III receptor) were tested in vitro and in vivo using different assays. Peptides P11 and P12, derived from TGF-beta1, and P54 and P144, derived from its type III receptor, prevented TGF-beta1-dependent inhibition of MV1Lu proliferation in vitro and markedly reduced binding of TGF-beta1 to its receptors. P144 blocked TGF-beta1-dependent stimulation of a reporter gene under the control of human alpha2(I) collagen promoter. Intraperitoneal administration of P144 also showed potent antifibrogenic activity in vivo in the liver of rats receiving CCl4. These rats also showed a significant decrease in the number of activated hepatic stellate cells as compared with those treated with saline only. These results suggest that short synthetic peptides derived from TGF-beta1 type III receptor may be of value in reducing liver fibrosis in chronic liver injury

Plasmonic antenna hybrids for active control in the near and midinfrared

Resumen del trabajo presentado a la Spanish Conference on Nanophotonics (Conferencia Española de Nanofotónica-CEN), celebrada en Donostia-San Sebastián (España) del 3 al 5 de octubre de 2018.Hybrid platforms combining metallic plasmonic nanoantennas (NAs) and materials with interesting properties as phase-change or spintronics offer excellent technological opportunities for active plasmonics, as they can provide large changes in their optical response. In this talk I will demostrate first how gold NAs grown on vanadium dioxie (VO2), characterized by a reversible insulator-to-metal transition (IMT) at around 68ºC, can improve the performance of this material by providing an efficient enhancement mechanism for both the optically induced excitation and readout. Using picosecond laser pulses a highly localized phase transition is driven in nanoscale regions around the NAs. These antennas-VO2 hybrid solutions provide a conceptual framework to merge field localization and phase transition enabling nanoscale optical memory functionalities. In the second part I will show how the combination of Au microantenna arrays with a Ni81Fe19/Au multilayer supports provide metamaterial platforms with new functionalities. In this case, the plasmon resonance sustained by the NAs alliate with the GMR and MRE effects of the multilayer to allow low magnetic-field controlled modulation in the mid-infrared, where light modulation is very challenging. This approach establishes a roadmap for spintronically-controlled devices in the whole mid-IR to THz band.Peer Reviewe

Diplopia Is Frequent and Associated with Motor and Non-Motor Severity in Parkinson’s Disease: Results from the COPPADIS Cohort at 2-Year Follow-Up

Malaltia de Parkinson; Fenotip; TremolorEnfermedad de Parkinson; Fenotipo; TemblorParkinson’s disease; Phenotype; TremorBackground and objective: Diplopia is relatively common in Parkinson’s disease (PD) but is still understudied. Our aim was to analyze the frequency of diplopia in PD patients from a multicenter Spanish cohort, to compare the frequency with a control group, and to identify factors associated with it. Patients and Methods: PD patients who were recruited from January 2016 to November 2017 (baseline visit; V0) and evaluated again at a 2-year ± 30 days follow-up (V2) from 35 centers of Spain from the COPPADIS cohort were included in this longitudinal prospective study. The patients and controls were classified as “with diplopia” or “without diplopia” according to item 15 of the Non-Motor Symptoms Scale (NMSS) at V0, V1 (1-year ± 15 days), and V2 for the patients and at V0 and V2 for the controls. Results: The frequency of diplopia in the PD patients was 13.6% (94/691) at V0 (1.9% in controls [4/206]; p < 0.0001), 14.2% (86/604) at V1, and 17.1% (86/502) at V2 (0.8% in controls [1/124]; p < 0.0001), with a period prevalence of 24.9% (120/481). Visual hallucinations at any visit from V0 to V2 (OR = 2.264; 95%CI, 1.269–4.039; p = 0.006), a higher score on the NMSS at V0 (OR = 1.009; 95%CI, 1.012–1.024; p = 0.015), and a greater increase from V0 to V2 on the Unified Parkinson’s Disease Rating Scale–III (OR = 1.039; 95%CI, 1.023–1.083; p < 0.0001) and Neuropsychiatric Inventory (OR = 1.028; 95%CI, 1.001–1.057; p = 0.049) scores were independent factors associated with diplopia (R2 = 0.25; Hosmer and Lemeshow test, p = 0.716). Conclusions: Diplopia represents a frequent symptom in PD patients and is associated with motor and non-motor severity.Solano Vila B. has received honoraria for educational presentations and advice service by UCB, Zambon, Teva, Abbvie, Bia

Diplopia is frequent and associated with motor and non-motor severity in parkinson's disease : Results from the COPPADIS cohort at 2-year follow-up

Background and objective: Diplopia is relatively common in Parkinson's disease (PD) but is still understudied. Our aim was to analyze the frequency of diplopia in PD patients from a multicenter Spanish cohort, to compare the frequency with a control group, and to identify factors associated with it. Patients and Methods: PD patients who were recruited from January 2016 to November 2017 (baseline visit; V0) and evaluated again at a 2-year ± 30 days follow-up (V2) from 35 centers of Spain from the COPPADIS cohort were included in this longitudinal prospective study. The patients and controls were classified as "with diplopia" or "without diplopia" according to item 15 of the Non-Motor Symptoms Scale (NMSS) at V0, V1 (1-year ± 15 days), and V2 for the patients and at V0 and V2 for the controls. Results: The frequency of diplopia in the PD patients was 13.6% (94/691) at V0 (1.9% in controls [4/206]; p < 0.0001), 14.2% (86/604) at V1, and 17.1% (86/502) at V2 (0.8% in controls [1/124]; p < 0.0001), with a period prevalence of 24.9% (120/481). Visual hallucinations at any visit from V0 to V2 (OR = 2.264; 95%CI, 1.269-4.039; p = 0.006), a higher score on the NMSS at V0 (OR = 1.009; 95%CI, 1.012-1.024; p = 0.015), and a greater increase from V0 to V2 on the Unified Parkinson's Disease Rating Scale-III (OR = 1.039; 95%CI, 1.023-1.083; p < 0.0001) and Neuropsychiatric Inventory (OR = 1.028; 95%CI, 1.001-1.057; p = 0.049) scores were independent factors associated with diplopia (R = 0.25; Hosmer and Lemeshow test, p = 0.716). Conclusions: Diplopia represents a frequent symptom in PD patients and is associated with motor and non-motor severity

Predictors of survival and toxicity in patients on adjuvant therapy with 5-fluorouracil for colorectal cancer

The present study aimed at investigating whether the simultaneous evaluation of pharmacokinetic, pharmacogenetic and demographic factors could improve prediction on toxicity and survival in colorectal cancer patients treated with adjuvant 5-fluorouracil (5FU)/leucovorin therapy. One hundred and thirty consecutive, B2 and C Duke's stage colorectal cancer patients were prospectively enrolled. 5FU pharmacokinetics was evaluated at the first cycle. Thymidylate synthase (TYMS) 5′UTR and 3′UTR polymorphisms and methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C polymorphisms were assessed in peripheral leukocytes. Univariate and multivariate analyses were applied to evaluate which variables could predict chemotherapy-induced toxicity, disease-free survival (DFS) and overall survival (OS). Multivariate analysis showed that: (a) low 5FU clearance was an independent predictive factor for severe toxicity (OR=7.32; P<0.0001); (b) high-5FU clearance predicted poorer DFS (HR=1.96; P=0.041) and OS (HR=3.37; P=0.011); (c) advanced age was associated with shorter DFS (HR=3.34; P=0.0008) and OS (HR=2.66; P=0.024); (d) the C/C genotype of the MTHFR C677T polymorphism was protective against grade 3–4 toxicity (P=0.040); (e) none of the TYMS polymorphisms could explain 5FU toxicity or clinical outcome

A retrospective observational study of the relationship between single nucleotide polymorphisms associated with the risk of developing Colorectal cancer and survival

Background: There is variability in clinical outcome for patients with apparently the same stage colorectal cancer (CRC). Single nucleotide polymorphisms (SNPs) mapping to chromosomes 1q41, 3q26.2, 6p21, 8q23.3, 8q24.21, 10p14, 11q13, 11q23.1, 12q13.13, 14q22, 14q22.2, 15q13.3, 16q22.1, 18q21.1, 19q13.11, 20p12, 20p12.3, 20q13.33 and Xp22 have robustly been shown to be associated with the risk of developing CRC. Since germline variation can also influence patient outcome the relationship between these SNPs and patient survivorship from CRC was examined. Methods: All enrolled into the National Study of Colorectal Cancer Genetics (NSCCG) were genotyped for 1q41, 3q26.2, 6p21, 8q23.3, 8q24.21, 10p14, 11q13, 11q23.1, 12q13.13, 14q22, 14q22.2, 15q13.3, 16q22.1, 18q21.1, 19q13.11, 20p12, 20p12.3, 20q13.33 and xp22 SNPs. Linking this information to the National Cancer Data Repository allowed patient genotype to be related to survival. Results: The linked dataset consisted of 4,327 individuals. 14q22.22 genotype defined by the SNP rs4444235 showed a significant association with overall survival. Specifically, the C allele was associated with poorer observed survival (per allele hazard ratio 1.13, 95% confidence interval 1.05-1.22, P = 0.0015). Conclusion: The CRC susceptibility SNP rs4444235 also appears to exert an influence in modulating patient survival and warrants further evaluation as a potential prognostic marker

Thymidylate synthase polymorphisms, folate and B-vitamin intake, and risk of colorectal adenoma

The effects of polymorphisms in genes coding for key folate metabolism enzymes such as thymidylate synthetase (TS) on colorectal neoplasia risk are likely to be influenced by gene–gene and gene–nutrient interactions. We investigated the combined effects of three polymorphisms in the TS gene region, TSER, TS 3R G>C, and TS 1494del6, dietary intakes of folate and other B vitamins, and genotype for other folate metabolism variants, in a colorectal adenoma (CRA) case–control study. Individuals homozygous for TS 1494del6 del/del were at significantly reduced CRA risk compared to those with either ins/del or ins/ins genotypes (odds ratio 0.52; 95% confidence interval: 0.31–0.85, P=0.009). We also observed evidence of interactions between TS 1494del6 genotype and intake of folate, and vitamins B6 and B12, and MTHFR C677T genotype, with the reduction in risk in del/del homozygotes being largely confined to individuals with high nutrient intakes and MTHFR 677CC genotype (Pinteraction=0.01, 0.006, 0.03, and 0.07, respectively). TSER genotype, when considered either alone or in combination with TS 3R G>C genotype, did not significantly influence CRA risk. These findings support a role for TS in colorectal carcinogenesis, and provide further evidence that functional polymorphisms in folate metabolism genes act as low-risk alleles for colorectal neoplasia and participate in complex gene–gene and gene–nutrient interactions