Heme oxygenase-1 induction in hepatocytes and non-parenchymal cells protects against liver injury during endotoxemia

INTRODUCTION:Heme oxygenase-1 (HO-1) is a stress response enzyme, which catalyses the breakdown of heme into biliverdin-IX alpha, carbon monoxide and ferrous iron. Under situations of oxidative stress, heat stress, ischemia/reperfusion injury or endotoxemia, HO-1 has been shown to be induced and to elicit a protective effect. The mechanism of how this protective effect is executed is unknown.RESULTS:HO-1 induction with cobalt protoporphorin (Co-PP) dose-dependently protected against apoptotic cell death as well as neutrophil-mediated oncosis in the galactosamine/endotoxin (Gal/ET) shock model. Induction of HO-1 with Co-PP dose-dependently protected against neutrophil-mediated oncosis as indicated by attenuated ALT release and TNF-mediated apoptotic cell death as indicated by reduced caspase-3 activation. HO-1 induction did not attenuate Gal/ET-induced TNF-alpha formation. Furthermore, a similar protective effect with the high dose of Co-PP was observed when animals were treated with Gal/TNF-alpha.CONCLUSIONS:HO-1 induction attenuates apoptosis and neutrophil-mediated oncosis in the Gal/ET shock model. However, the protective effect is not due to the reduction of TNF-alpha release or the attenuation of neutrophil accumulation in the liver sinusoids.This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at [email protected]

Ultraviolet Imaging of the Globular Cluster 47 Tucanae

We have used the Ultraviolet Imaging Telescope to obtain deep far-UV (1620 Angstrom), 40' diameter images of the prototypical metal-rich globular cluster 47 Tucanae. We find a population of about 20 hot (Teff > 9000 K) objects near or above the predicted UV luminosity of the hot horizontal branch (HB) and lying within two half-light radii of the cluster center. We believe these are normal hot HB or post-HB objects rather than interacting binaries or blue stragglers. IUE spectra of two are consistent with post-HB phases. These observations, and recent HST photometry of two other metal-rich clusters, demonstrate that populations with rich, cool HB's can nonetheless produce hot HB and post-HB stars. The cluster center also contains an unusual diffuse far-UV source which is more extended than its V-band light. It is possible that this is associated with an intracluster medium, for which there was earlier infrared and X-ray evidence, and is produced by C IV emission or scattered light from grains.Comment: 13 pages AASLaTeX including one postscript figure and one bitmapped image, JPEG format. Submitted to the Astronomical Jorunal. Full Postscript version available at http://www.astro.virginia.edu/~bd4r

Risk for Hospital Readmission following Bariatric Surgery

Background and Objectives: Complications resulting in hospital readmission are important concerns for those considering bariatric surgery, yet present understanding of the risk for these events is limited to a small number of patient factors. We sought to identify demographic characteristics, concomitant morbidities, and perioperative factors associated with hospital readmission following bariatric surgery. Methods: We report on a prospective observational study of 24,662 patients undergoing primary RYGB and 26,002 patients undergoing primary AGB at 249 and 317 Bariatric Surgery Centers of Excellence (BSCOE), respectively, in the United States from January 2007 to August 2009. Data were collected using standardized assessments of demographic factors and comorbidities, as well as longitudinal records of hospital readmissions, complications, and mortality. Results: The readmission rate was 5.8 % for RYGB and 1.2 % for AGB patients 30 days after discharge. The greatest predictors for readmission following RYGB were prolonged length of stay (adjusted odds ratio [OR], 2.3; 95 % confidence interval [CI]

UIT Detection of Hot Stars in the Globular Cluster NGC362

We used the Ultraviolet Imaging Telescope during the March 1995 Astro-2 mission to obtain a deep far-UV image of the globular cluster NGC 362, which was formerly thought to have an almost entirely red horizontal branch (HB). 84 hot (T_eff > 8500 K) stars were detected within a radius of 8'.25 of the cluster center. Of these, 43 have FUV magnitudes consistent with HB stars in NGC 362, and at least 34 are cluster members. The number of cluster members is made uncertain by background contamination from blue stars in the Small Magellanic Cloud (SMC). There are six candidate supra-HB stars which have probably evolved from the HB. We discuss the implications of these results for the production of hot blue stars in stellar populations.Comment: 10 pages AASLaTeX including one postscript figure and one compressed bitmap, .jpg format. To appear in Ap. J. Letters. Postscript version also available at http://www.astro.virginia.edu/~bd4r

HST Observations of New Horizontal Branch Structures in the Globular Cluster omega Centauri

The globular cluster omega Centauri contains the largest known population of very hot horizontal branch (HB) stars. We have used the Hubble Space Telescope to obtain a far-UV/optical color-magnitude diagram of three fields in omega Cen. We find that over 30% of the HB objects are ``extreme'' HB or hot post-HB stars. The hot HB stars are not concentrated toward the cluster center, which argues against a dynamical origin for them. A wide gap in the color distribution of the hot HB stars appears to correspond to gaps found earlier in several other clusters. This suggests a common mechanism, probably related to giant branch mass loss. The diagram contains a significant population of hot sub-HB stars, which we interpret as the ``blue-hook'' objects predicted by D'Cruz et al. (1996a). These are produced by late He-flashes in stars which have undergone unusually large giant branch mass loss. omega Cen has a well-known spread of metal abundance, and our observations are consistent with a giant branch mass loss efficiency which increases with metallicity.Comment: Submitted to ApJ, 12 pages, including 3 figures, also available at http://www.physics.usyd.edu.au/~noella/research.htm

Detection of RNA from a Novel West Nile-like Virus and High Prevalence of an Insect-specific Flavivirus in Mosquitoes in the Yucatan Peninsula of Mexico

As part of our ongoing surveillance efforts for West Nile virus (WNV) in the Yucatan Peninsula of Mexico, 96,687 mosquitoes collected from January through December 2007 were assayed by virus isolation in mammalian cells. Three mosquito pools caused cytopathic effect. Two isolates were orthobunyaviruses (Cache Valley virus and Kairi virus) and the identity of the third infectious agent was not determined. A subset of mosquitoes was also tested by reverse transcription-polymerase chain reaction (RT-PCR) using WNV-, flavivirus-, alphavirus-, and orthobunyavirus-specific primers. A total of 7,009 Culex quinquefasciatus in 210 pools were analyzed. Flavivirus RNA was detected in 146 (70%) pools, and all PCR products were sequenced. The nucleotide sequence of one PCR product was most closely related (71-73% identity) with homologous regions of several other flaviviruses, including WNV, St. Louis encephalitis virus, and Ilheus virus. These data suggest that a novel flavivirus (tentatively named T\u27Ho virus) is present in Mexico. The other 145 PCR products correspond to Culex flavivirus, an insect-specific flavivirus first isolated in Japan in 2003. Culex flavivirus was isolated in mosquito cells from approximately one in four homogenates tested. The genomic sequence of one isolate was determined. Surprisingly, heterogeneous sequences were identified at the distal end of the 5\u27 untranslated region

Ultraviolet Imaging of the z=0.23 Cluster Abell 2246

We present deep ultraviolet observations of a field containing the cluster Abell 2246 (z=0.225) which provide far-ultraviolet (FUV) images of some of the faintest galaxies yet observed in that bandpass. Abell 2246 lies within the field of view of Ultraviolet Imaging Telescope (UIT) observations of the quasar HS1700+64, which accumulated over 7100 seconds of UIT FUV exposure time during the Astro-2 mission in March 1995. For objects found on both the FUV and ground-based V-band images, we obtain FUV (l ~ 1520 A) photometry and V-band photometry, as well as mid-UV (l ~ 2490 A) photometry from UIT Astro-1 observations and ground-based I-band photometry. We find five objects in the images which are probably galaxies at the distance of Abell 2246, with FUV magnitudes (m(FUV)) between 18.6 and 19.6, and V magnitudes between 18.4 and 19.6. We find that their absolute FUV fluxes and colors imply strongly that they are luminous galaxies with significant current star formation, as well as some relatively recent, but not current, (> 400 Myr ago) star formation. We interpret the colors of these five objects by comparing them with local objects, redshift-corrected template spectra and stellar population models, finding that they are plausibly matched by 10-Gyr-old population models with decaying star formation, with decay time constants in the range 3 Gyr < t < 5 Gyr, with an additional color component from a single burst of moderate ( ~ 400-500 Myr) age. From derived FUV luminosities we compute current star formation rates. We compare the UV properties of Abell 2246 with those of the Coma cluster, finding that Abell 2246 has significantly more recent star formation, consistent with the Butcher-Oemler phenomenon.Comment: Accepted for publication in the Astronomical Journal, June 1998. 17 Pages AAS latex, includes 4 bitmap .jpg format images and 4 other figures. PDF, Embedded Gzipped PS version (1.9Mb) TeX source and figures available at http://www.astro.virginia.edu/~bd4r/galaxies.htm

Multimodal Distributions along the Horizontal Branch

We report on HST/WFPC2 U,V and far-ultraviolet observations of two Galactic Globular Clusters (GGCs), NGC 6205 = M13 and NGC 6093 = M80. Both of these clusters have horizontal-branch (HB) tails that extend to the helium-burning main sequence, with the hottest stars reaching theoretical effective temperatures above 35,000 K. In both clusters, groups of stars are found to be separated by narrow gaps along the blue HB sequence. These gaps appear at similar locations in the color-magnitude diagrams of the two clusters. While stochastic effects may give rise to variations in the color distribution along the HB, the coincidence of gaps in different clusters effectively rules this out as the primary cause. The comparison among the clusters strongly suggests that there are separate physical processes operating during the earlier red-giant phase of evolution to produce mass loss.Comment: 13pp LaTeX, uses AAS Latex version 4. Includes 7 postscript figures. Postscript file available for download at http://www.astro.virginia.edu/~bd4

Pest population dynamics are related to a continental overwintering gradient

Overwintering success is an important determinant of arthropod populations that must be considered as climate change continues to influence the spatiotemporal population dynamics of agricultural pests. Using a long-term monitoring database and biologically relevant overwintering zones, we modeled the annual and seasonal population dynamics of a common pest, Helicoverpa zea (Boddie), based on three overwintering suitability zones throughout North America using four decades of soil temperatures: the southern range (able to persist through winter), transitional zone (uncertain overwintering survivorship), and northern limits (unable to survive winter). Our model indicates H. zea population dynamics are hierarchically structured with continental-level effects that are partitioned into three geographic zones. Seasonal populations were initially detected in the southern range, where they experienced multiple large population peaks. All three zones experienced a final peak between late July (southern range) and mid-August to mid-September (transitional zone and northern limits). The southern range expanded by 3% since 1981 and is projected to increase by twofold by 2099 but the areas of other zones are expected to decrease in the future. These changes suggest larger populations may persist at higher latitudes in the future due to reduced low-temperature lethal events during winter. Because H. zea is a highly migratory pest, predicting when populations accumulate in one region can inform synchronous or lagged population development in other regions. We show the value of combining long-term datasets, remotely sensed data, and laboratory findings to inform forecasting of insect pests