Forested wetlands for water resource management in southern Illinois

A 30 ha cypress - tupelo (Taxodium distichum - Nyssa aquatics), floodplain swamp in Southern Illinois was studied for its hydrologic, biogeochemical and ecological characteristics. The hydrology, water chemistry, sediment dynamics and ecosystem productivity were described for the swamp and interactions with the adjacent Cache River were emphasized. A representative flood in the spring spilled water and sediments from the river to the swamp, temporarily reversing the normal flow of water from the swamp to the river. The annual hydrology budget for the swamp showed inflows of 7'4.4 cm throughfall, 63.9 cm runoff, and 21.9 cm groundwater; the outflows were 7'2.8 cm evapotranspiration, 54.9 cm surface outflow, and 21.0 cm groundwater, the latter two draining to the river. Loading rates for several chemical parameters were calculated from the swamp to the river and water chemistry of the swamp and river was contrasted. Primary productivity measurements showed high rates when the floating duckweed was included; cypress productivity was shown historically to be related to amount of flooding. A phosphorus budget was determined for the swamp and this indicated that the flooding river contributed over 10 times the phosphorus to the swamp as was discharged the rest of the year.U.S. Department of the InteriorU.S. Geological SurveyOpe

SIVagm Infection in Wild African Green Monkeys from South Africa: Epidemiology, Natural History, and Evolutionary Considerations

Pathogenesis studies of SIV infection have not been performed to date in wild monkeys due to difficulty in collecting and storing samples on site and the lack of analytical reagents covering the extensive SIV diversity. We performed a large scale study of molecular epidemiology and natural history of SIVagm infection in 225 free-ranging AGMs from multiple locations in South Africa. SIV prevalence (established by sequencing pol, env, and gag) varied dramatically between infant/juvenile (7%) and adult animals (68%) (p<0.0001), and between adult females (78%) and males (57%). Phylogenetic analyses revealed an extensive genetic diversity, including frequent recombination events. Some AGMs harbored epidemiologically linked viruses. Viruses infecting AGMs in the Free State, which are separated from those on the coastal side by the Drakensberg Mountains, formed a separate cluster in the phylogenetic trees; this observation supports a long standing presence of SIV in AGMs, at least from the time of their speciation to their Plio-Pleistocene migration. Specific primers/probes were synthesized based on the pol sequence data and viral loads (VLs) were quantified. VLs were of 104-106 RNA copies/ml, in the range of those observed in experimentally-infected monkeys, validating the experimental approaches in natural hosts. VLs were significantly higher (107-108 RNA copies/ml) in 10 AGMs diagnosed as acutely infected based on SIV seronegativity (Fiebig II), which suggests a very active transmission of SIVagm in the wild. Neither cytokine levels (as biomarkers of immune activation) nor sCD14 levels (a biomarker of microbial translocation) were different between SIV-infected and SIV-uninfected monkeys. This complex algorithm combining sequencing and phylogeny, VL quantification, serology, and testing of surrogate markers of microbial translocation and immune activation permits a systematic investigation of the epidemiology, viral diversity and natural history of SIV infection in wild African natural hosts. © 2013 Ma et al

Current in vivo models of varicella-zoster virus neurotropism

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. Varicella-zoster virus (VZV), an exclusively human herpesvirus, causes chickenpox and establishes a latent infection in ganglia, reactivating decades later to produce zoster and associated neurological complications. An understanding of VZV neurotropism in humans has long been hampered by the lack of an adequate animal model. For example, experimental inoculation of VZV in small animals including guinea pigs and cotton rats results in the infection of ganglia but not a rash. The severe combined immune deficient human (SCID-hu) model allows the study of VZV neurotropism for human neural sub-populations. Simian varicella virus (SVV) infection of rhesus macaques (RM) closely resembles both human primary VZV infection and reactivation, with analyses at early times after infection providing valuable information about the extent of viral replication and the host immune responses. Indeed, a critical role for CD4 T-cell immunity during acute SVV infection as well as reactivation has emerged based on studies using RM. Herein we discuss the results of efforts from different groups to establish an animal model of VZV neurotropism

SOx trapping performances of cuo based silica mesoporous adsorbents for desulfurization of industrial flue gas stream

In the present work, CuO/SBA-15 SOx regenerable adsorbents were elaborated. Synthesis conditions were controlled in order to obtain highly dispersed Cu2+ species assumed to be Cu-O-Si species. Materials were evaluated as SOx adsorbents through multicycle adsorption/regeneration experiments. Their performances decrease along cycles due to copper species sintering and there is an optimal copper loading for a maximal SOx adsorption efficiency. Please click Additional Files below to see the full abstract

Simian varicella virus infection of Chinese rhesus macaques produces ganglionic infection in the absence of rash

Varicella-zoster virus (VZV) causes varicella (chickenpox), becomes latent in ganglia along the entire neuraxis, and may reactivate to cause herpes zoster (shingles). VZV may infect ganglia via retrograde axonal transport from infected skin or through hematogenous spread. Simian varicella virus (SVV) infection of rhesus macaques provides a useful model system to study the pathogenesis of human VZV infection. To dissect the virus and host immune factors during acute SVV infection, we analyzed four SVV-seronegative Chinese rhesus macaques infected intratracheally with cell-associated 5 × 103 plaque-forming units (pfu) of SVV-expressing green fluorescent protein (n = 2) or 5 × 104 pfu of wild-type SVV (n = 2). All monkeys developed viremia and SVV-specific adaptive B- and T-cell immune responses, but none developed skin rash. At necropsy 21 days postinfection, SVV DNA was found in ganglia along the entire neuraxis and in viscera, and SVV RNA was found in ganglia, but not in viscera. The amount of SVV inoculum was associated with the extent of viremia and the immune response to virus. Our findings demonstrate that acute SVV infection of Chinese rhesus macaques leads to ganglionic infection by the hematogenous route and the induction of a virus-specific adaptive memory response in the absence of skin rash

SOx trapping performances of cuo based silica mesoporous adsorbents for desulfurization of industrial flue gas stream

In the present work, CuO/SBA-15 SOx regenerable adsorbents were elaborated. Synthesis conditions were controlled in order to obtain highly dispersed Cu2+ species assumed to be Cu-O-Si species. Materials were evaluated as SOx adsorbents through multicycle adsorption/regeneration experiments. Their performances decrease along cycles due to copper species sintering and there is an optimal copper loading for a maximal SOx adsorption efficiency. Please click Additional Files below to see the full abstract