Condition of K-12 Public Education in Maine 2009

This twelfth edition of The Condition of K-12 Public Education in Maine is designed to provide Maine citizens, legislators, and educators a yearly report on the state of Maine public schools and education. This new edition updates educational information which appeared in earlier editions, and also provides information on several new topics. Education Indicators are facts and statistics that help to describe a public education system. They are tools which are useful in examining and measuring the effectiveness of the system. Examples include information such as the amount of local funds raised to support local schools, per pupil expenditures, pupil-teacher ratios, and student achievement results. This publication contains a series of indicators which will help interested citizens, policymakers, and legislators understand the many components of the K-12 Maine public education system. This edition is comprised of six categories of indicators: (1) Background Demographics; (2) Enrollment; (3) Staff; (4) Program; (5) Student Performance; and (6) Finance. While the categories have been changed recently from previous editions, the report still contains the same indicators. The Background Demographics section provides information on community and societal characteristics of the education environment which may have an impact on student learning. The Enrollment section highlights enrollment trends statewide and in some cases by county. The Staff section provides characteristics of Teachers and Administrators in schools statewide. The Program section provides information on the school district organizational structure and other specific programs within schools that enhance education in Maine. The Student Performance section provides a tool to assess the productivity and accomplishments of education in Maine. And finally, the Finance section provides financial information relevant to education in Maine. Appended are: (1) Statutory Language for the Maine Education Policy Research Institute Title 20-A Chapter 1 Section 10, MRSA; and (2) Recent Publications. (Contains 55 tables and 39 figures.

School District Reorganization in Maine: Lessons Learned for Policy and Process

In 2007, Maine’s legislature enacted a law mandating school district consolidation with the goal of reducing the state’s 290 districts to approximately 80. Five years later the success of this policy is open to debate. Janet Fairman and Christine Donis-Keller examine what worked and what didn’t work in this effort to consolidate school districts and provide a list of “lessons learned,” with clear implications for the design and implementation of state educational policy

Improving Educational Opportunity and Equity through School District Consolidation in Maine

In 2007 Maine passed sweeping school district consolidation legislation mandating a reduction in the number of Maine school districts from 290 to approximately 80. The primary goals of the policy were to improve the educational opportunities for all students in the state; and to reduce costs through increased efficiency in the delivery of education programs and services. Based primarily on interviews with district leaders, this article describes the impacts of Maine’s school district consolidation policy on educational opportunities and equity within 24 regional school districts, one year after their mergers. Findings illustrate the different choices districts made when consolidating their educational programs, the outcomes of these efforts, and the strategies and structures districts used to implement change

The Condition of K-12 Public Education in Maine 2011

This book is designed to provide Maine citizens, legislators, and educators a bi-annual report on the state of Maine public schools and education. This new edition updates educational information which appeared in earlier editions, and also provides information on several new topics

Chromosomal bar codes produced by multicolor fluorescence in situ hybridization with multiple YAC clones and whole chromosome painting probes

Colored chromosome staining patterns, termed chromosomal ‘bar codes’ (CBCs), were obtained on human chromosomes by fluorescence in situ hybridization (FISH) with pools of Alu-PCR products from YAC dones containing human DNA inserts ranging from 100 kbp to 1 Mbp. In contrast to conventional G- or R-bands, the chromosomal position, extent, Individual color and relative signal intensity of each ‘bar’ could be modified depending on probe selection and labeling procedures. Alu-PCR amplification products were generated from 31 YAC clones which mapped to 37 different chromosome bands. For multiple color FISH, Alu-PCR amplification products from various clones were either biotinylated or labeled with digoxigenin. Probes from up to twenty YAC clones were used simultaneously to produce CBCs on selected human chromosomes. Evaluation using a cooled CCD camera and digital image analysis confirmed the high reproducibility of the bars from one metaphase spread to another. Combinatorial FISH with mixtures of whole chromosome paint probes was applied to paint seven chromosomes simultaneously in different colors along with a set of YAC clones which map to these chromosomes. We discuss the potential to construct analytical chromosomal bar codes adapted to particular needs of cytogenetic investigations and automated image analysis

Isolation of YAC Clones From the Pericentromeric Region of Chromosome 10 and Development of New Genetic Markers Linked to the Multiple Endocrine Neoplasia Type 2A Gene

Genetic linkage mapping and contig assembly using yeast artificial chromosome (YAC) technology form the basis of our strategy to clone and define the genomic structure of the pericentromeric region of chromosome 10 containing the multiple endocrine neoplasia type 2A gene. Thus far YAC walks have been initiated from five chromosome 10 pericentromeric loci including RBP3, D10S94, RET, D10Z1, and FNRB. Long range pulsed-field gel electrophoresis maps are constructed from the YACs isolated to define clone overlaps and to identify putative CpG islands. Bidirectional YAC walks are continued by rescreening the YAC library with sequence-tagged site assays developed from endclones. Several new restriction fragment length polymorphisms and simple sequence repeat polymorphism markers have been identified from the YAC clones. In particular, two highly informative (CA)n dinucleotide repeat markers, sTCL-1 from proximal chromosome 10p (16 alleles, PIC = 0.68) and sJRH-1 from the RBP3 locus (18 alleles. PIC = 0.88), provide useful reagents for a polymerase chain reaction-based predictive genetic test that can be performed rapidly from small amounts of DNA

A strategy for the characterization of minute chromosome rearrangements using multiple color fluorescence in situ hybridization with chromosome-specific DNA libraries and YAC clones

The identification of marker chromosomes in clinical and tumor cytogenetics by chromosome banding analysis can create problems. In this study, we present a strategy to define minute chromosomal rearrangements by multicolor fluorescence in situ hybridization (FISH) with whole chromosome painting probes derived from chromosome-specific DNA libraries and Alu-polymerase chain reaction (PCR) products of various region-specific yeast artificial chromosome (YAC) clones. To demonstrate the usefulness of this strategy for the characterization of chromosome rearrangements unidentifiable by banding techniques, an 8p+ marker chromosome with two extra bands present in the karyotype of a child with multiple anomalies, malformations, and severe mental retardation was investigated. A series of seven-color FISH experiments with sets of fluorochrome-labeled DNA library probes from flow-sorted chromosomes demonstrated that the additional segment on 8p+ was derived from chromosome 6. For a more detailed characterization of the marker chromosome, three-color FISH experiments with library probes specific to chromosomes 6 and 8 were performed in combination with newly established telomeric and subtelomeric YAC clones from 6q25, 6p23, and 8p23. These experiments demonstrated a trisomy 6pter6p22 and a monosomy 8pter8p23 in the patient. The present limitations for a broad application of this strategy and its possible improvements are discusse