A Dual-Fluorescent Composite of Graphene Oxide and Poly(3-Hexylthiophene) Enables the Ratiometric Detection of Amines

A composite prepared by grafting a conjugated polymer, poly(3-hexylthiophene) (P3HT), to the surface of graphene oxide was shown to result in a dual-fluorescent material with tunable photoluminescent properties. Capitalizing on these unique features, a new class of graphene-based sensors that enables the ratiometric fluorescence detection of amine-based pollutants was developed. Moreover, through a detailed spectroscopic study, the origin of the optical properties of the aforementioned composite was studied and was found to be due to electronic decoupling of the conjugated polymer from the GO. The methodology described herein effectively overcomes a long-standing challenge that has prevented graphene based composites from finding utility in sensing and related applications.Meng, Dongli, Shaojun Yang, Dianming Sun, Yi Zeng, Jinhua Sun, Yi Li, Shouke Yan, Yong Huang, Christopher W. Bielawski, and Jianxin Geng. "A dual-fluorescent composite of graphene oxide and poly (3-hexylthiophene) enables the ratiometric detection of amines." Chemical Science 5, no. 8 (Apr., 2014): 3130-3134.Chemistr

Epigenetic regulation of CD271, a potential cancer stem cell marker associated with chemoresistance and metastatic capacity.

Cancer stem cells (CSCs) are considered to be the cause of tumor initiation, metastasis and recurrence. Additionally, CSCs are responsible for the failure of chemotherapy and radiotherapy. The isolation and identification of CSCs is crucial for facilitating the monitoring, therapy or prevention of cancer. We aimed to identify esophageal squamous cell carcinoma (ESCC) stem-like cells, the epigenetic mechanism and identify novel biomarkers for targeting ESCC CSCs. Sixty-three paired ESCC tissues and adjacent non-cancerous tissues were included in this study. CD271, which was identified as the CSC marker for melanoma, was assessed using quantitative PCR (qPCR). Using flow cytometry, we isolated CD271+ cells comprising 7.5% of cancer cells from the KYSE70 cell line. Sphere formation and anchorage-independent growth were analyzed in CD271+ and CD271- cancer cells, respectively. qPCR was used to detect stem-related genes and CCK-8 was performed to analyze the sensitivity to chemotherapy in the two groups. Bisulfite genomic sequencing was used to analyze the methylation status. CD271 expression was significantly higher in ESCC tissues than in adjacent non-cancerous tissues. Compared with CD271- cancer cells, CD271+ cancer cells showed a higher ability of sphere and colony formation, a high level expression of stem-related gene, and resistance to chemotherapy. The expression of CD271 was induced by a demethylation agent. In conclusion, CD271+ ESCC cells possess stem-like properties. CD271 can potentially act as a prognostic marker for ESCC, whose expression is regulated epigenetically

Report drawn up on behalf of the Committee on Economic and Monetary Affairs on the possible loan from the OPEC countries to the Federal Republic of Germany and to France. EP Working Documents 1982-83, Document 1-284/82, 4 June 1982

Abstract Background Hand, foot, and mouth disease (HFMD) has become an emerging infectious disease in China in the last decade. There has been evidence that meteorological factors can influence the HFMD incidence, and understanding the mechanisms can help prevent and control HFMD. Methods HFMD incidence data and meteorological data in Minhang District, Shanghai were obtained for the period between 2009 and 2015. Distributed lag non-linear models (DLNMs) were utilized to investigate the impact of meteorological factors on HFMD incidence after adjusting for potential confounders of long time trend, weekdays and holidays. Results There was a non-linear relationship between temperature and HFMD incidence, the RR of 5th percentile compared to the median is 0.836 (95% CI: 0.671–1.042) and the RR of 95th percentile is 2.225 (95% CI: 1.774–2.792), and the effect of temperature varied across age groups. HFMD incidence increased with increasing average relative humidity (%) (RR = 1.009, 95% CI: 1.005–1.015) and wind speed (m/s) (RR = 1.197, 95% CI: 1.118–1.282), and with decreasing daily rainfall (mm) (RR = 0.992, 95% CI: 0.987–0.997) and sunshine hours (h) (RR = 0.966, 95% CI: 0.951–0.980). Conclusions There were significant relationships between meteorological factors and childhood HFMD incidence in Minhang District, Shanghai. This information can help local health agencies develop strategies for the control and prevention of HFMD under specific climatic conditions

Constructing a prognostic model for hepatocellular carcinoma based on bioinformatics analysis of inflammation-related genes

BackgroundThis study aims to screen inflammation-related genes closely associated with the prognosis of hepatocellular carcinoma (HCC) to accurately forecast the prognosis of HCC patients.MethodsGene expression matrices and clinical information for liver cancer samples were obtained from the Cancer Genome Atlas (TCGA) and the International Cancer Genome Consortium (ICGC). An intersection of differentially expressed genes of HCC and normal and GeneCards yielded inflammation-related genes associated with HCC. Cox regression and the minor absolute shrinkage and selection operator (LASSO) regression analysis to filter genes associated with HCC prognosis. The prognostic value of the model was confirmed by drawing Kaplan–Meier and ROC curves. Select differentially expressed genes between the high-risk and low-risk groups and perform GO and KEGG pathways analyses. CIBERSORT analysis was conducted to assess associations of risk models with immune cells and verified using real-time qPCR.ResultsA total of six hub genes (C3, CTNNB1, CYBC1, DNASE1L3, IRAK1, and SERPINE1) were selected using multivariate Cox regression to construct a prognostic model. The validation evaluation of the prognostic model showed that it has an excellent ability to predict prognosis. A line plot was drawn to indicate the HCC patients’ survival, and the calibration curve revealed satisfactory predictability. Among the six hub genes, C3 and DNASE1L3 are relatively low expressed in HCCLM3 and 97H liver cancer cell lines, while CTNNB1, CYBC1, IRAK1, and SERPINE1 are relatively overexpressed in liver cancer cell lines.ConclusionOne new inflammatory factor-associated prognostic model was constructed in this study. The risk score can be an independent predictor for judging the prognosis of HCC patients’ survival

The GECAM Real-Time Burst Alert System

Gravitational Wave High-energy Electromagnetic Counterpart All-sky Monitor (GECAM), consisting of two micro-satellites, is designed to detect gamma-ray bursts associated with gravitational-wave events. Here, we introduce the real-time burst alert system of GECAM, with the adoption of the BeiDou-3 short message communication service. We present the post-trigger operations, the detailed ground-based analysis, and the performance of the system. In the first year of the in-flight operation, GECAM was triggered by 42 GRBs. GECAM real-time burst alert system has the ability to distribute the alert within $\sim$1 minute after being triggered, which enables timely follow-up observations.Comment: 17 pages, 10 figures; Accepted for publication in RA

Compressed glassy carbon: An ultrastrong and elastic interpenetrating graphene network

Carbon’s unique ability to have both sp2 and sp3 bonding states gives rise to a range of physical attributes, including excellent mechanical and electrical properties. We show that a series of lightweight, ultrastrong, hard, elastic, and conductive carbons are recovered after compressing sp2-hybridized glassy carbon at various temperatures. Compression induces the local buckling of graphene sheets through sp3 nodes to form interpenetrating graphene networks with long-range disorder and short-range order on the nanometer scale. The compressed glassy carbons have extraordinary specific compressive strengths—more than two times that of commonly used ceramics—and simultaneously exhibit robust elastic recovery in response to local deformations. This type of carbon is an optimal ultralight, ultrastrong material for a wide range of multifunctional applications, and the synthesis methodology demonstrates potential to access entirely new metastable materials with exceptional properties

Chk1 Inhibition Ameliorates Alzheimer's Disease Pathogenesis and Cognitive Dysfunction Through CIP2A/PP2A Signaling

Alzheimer's disease (AD) is the most common neurodegenerative disease with limited therapeutic strategies. Cell cycle checkpoint protein kinase 1 (Chk1) is a Ser/Thr protein kinase which is activated in response to DNA damage, the latter which is an early event in AD. However, whether DNA damage-induced Chk1 activation participates in the development of AD and Chk1 inhibition ameliorates AD-like pathogenesis remain unclarified. Here, we demonstrate that Chk1 activity and the levels of protein phosphatase 2A (PP2A) inhibitory protein CIP2A are elevated in AD human brains, APP/PS1 transgenic mice, and primary neurons with A beta treatment. Chk1 overexpression induces CIP2A upregulation, PP2A inhibition, tau and APP hyperphosphorylation, synaptic impairments, and cognitive memory deficit in mice. Moreover, Chk1 inhibitor (GDC0575) effectively increases PP2A activity, decreases tau phosphorylation, and inhibits A beta overproduction in AD cell models. GDC0575 also reverses AD-like cognitive deficits and prevents neuron loss and synaptic impairments in APP/PS1 mice. In conclusion, our study uncovers a mechanism by which DNA damage-induced Chk1 activation promotes CIP2A-mediated tau and APP hyperphosphorylation and cognitive dysfunction in Alzheimer's disease and highlights the therapeutic potential of Chk1 inhibitors in AD

Structural Basis of Chemokine Sequestration by CrmD, a Poxvirus-Encoded Tumor Necrosis Factor Receptor

Pathogens have evolved sophisticated mechanisms to evade detection and destruction by the host immune system. Large DNA viruses encode homologues of chemokines and their receptors, as well as chemokine-binding proteins (CKBPs) to modulate the chemokine network in host response. The SECRET domain (smallpox virus-encoded chemokine receptor) represents a new family of viral CKBPs that binds a subset of chemokines from different classes to inhibit their activities, either independently or fused with viral tumor necrosis factor receptors (vTNFRs). Here we present the crystal structures of the SECRET domain of vTNFR CrmD encoded by ectromelia virus and its complex with chemokine CX3CL1. The SECRET domain adopts a β-sandwich fold and utilizes its β-sheet I surface to interact with CX3CL1, representing a new chemokine-binding manner of viral CKBPs. Structure-based mutagenesis and biochemical analysis identified important basic residues in the 40s loop of CX3CL1 for the interaction. Mutation of corresponding acidic residues in the SECRET domain also affected the binding for other chemokines, indicating that the SECRET domain binds different chemokines in a similar manner. We further showed that heparin inhibited the binding of CX3CL1 by the SECRET domain and the SECRET domain inhibited RAW264.7 cell migration induced by CX3CL1. These results together shed light on the structural basis for the SECRET domain to inhibit chemokine activities by interfering with both chemokine-GAG and chemokine-receptor interactions

Development of a generic framework for dynamic website design

Nowadays most of the web pages were dynamic. It meant that there was a database at the backend. Once a change was made in database, a dynamic change on the website would show. Also web pages used content management system (CMS) techniques to help non-technical administrators manage the web site easily and administrator-friendly. The purpose of the project was to implement a dynamic, CMS based, administrator friendly website. Our aim was to help the managers edit, add new, delete items on the website easily. The scenario of the project was to build a website for iEEE Photonics Society Singapore Chapter. At last, we expected to implement a generic web framework that other small organizations with similar requirement could just use the developed applications to install a dynamic website with simply installation and configuration the developed components of the website. We used Joomla! as our platform and our basic framework to start with. At last, two Joomla! components were developed and installed. The two components were for event management and committee member management. We implemented the components to achieve the functionalities of editing, adding new, deleting items, as well as registering events and mail function to inform registered user or administrators upon activity succeeded. Other functionalities that were required but beyond our main scope were also realized by installing existing Joomla! extensions. A deep search for suitable extensions was done. At last, the website was successful implemented and installed in a real web server.Bachelor of Engineerin