Puukohola Heiau National Historic Site plant survey

Reports were scanned in black and white at a resolution of 600 dots per inch and were converted to text using Adobe Paper Capture Plug-in.A checklist of all plant species located within the Puukohola Heiau National Historic Site was compiled for the dry season during May 1975, and for the wet season in March 1976. A total of 50 species from 21 Angiosperm families were recorded along with 2 ferns and 1 Basidiomycete. Of the total number of species collected, 38 were introductions, 10 were indigenous, and 5 were endemic. Among the species present are Ophioglossum concinnum, a fern proposed for rare and endangered status, and an unknown species of Atriplex, provisionally identified as A. johnstonii. Four distinct communities were recognized: a xerophytic scrub savannah, a closed kiawe forest, a halophytic scrub, and a disturbed roadside community. Recommendations are made concerning a potential fire hazard, the control of two noxious weeds, and the preservation of the rare fern.National Park Service Contract No. CX 8000 6 003

Ultrastructure and physiology of germination in Phytophthora parasitica

Typescript.Thesis (Ph. D.)--University of Hawaii, 1970.Bibliography: leaves [190]-196.x, 196 l illu

The physiography and marine fauna of inshore and intertidal areas in the Puukohola Heiau National Historic Site

Reports were scanned in black and white at a resolution of 600 dots per inch and were converted to text using Adobe Paper Capture Plug-in.This report describes the physiography and marine fauna of waters enclosed by the boundaries of the Puukohola Heiau National Historic Site from the high tide mark to approximately 150 m from shore. Line transect and qualitative methods were employed to sample the benthic and demersal macrofauna within biotopes ranging from brackish pools to coral patch reefs. Spot checks were also made outside the survey site for comparative purposes. With few exceptions, the reef ecosystem within the site is depauperate. About half as many species of fish (63) were observed as were previously reported (111 species) in an area a few hundred meters seaward of the site. Grey reef, blacktip, and whitetip reef sharks were commonly seen breaking the surface near the presumed site of the Hale o Kapuni heiau. The sharks may have been attracted by the warm waters of the site and/or to the large schools of juvenile mullet seen within these waters. Striking gradients in diversity and distribution of benthic invertebrates were typically correlated with substrate and water quality factors. Corals in the site were under moderate to heavy siltation stress and inshore areas were dominated by opportunistic species. Recommendations to stabilize and improve the reef ecosystem are given.National Park Service Contract No. CX 8000 6 003

Pu-u Kohol? Heiau National Historic Site plant survey

Western Region, National Park Service, Contract #CX8000 6 003

Data from: Investigating the allelic evolution of an imperfect microsatellite locus in the Hawaiian mushroom Rhodocollybia laulaha

The evolutionary mechanisms that give rise to microsatellite alleles remain poorly understood in general and are especially understudied for fungal microsatellite loci. The unusual G28 microsatellite locus was developed from the Hawaiian mushroom Rhodocollybia laulaha. Here we employ a novel approach to test for allele size homoplasy and examine competing mechanistic models of microsatellite evolution in the context of biogeographic expectations for this locus based on Hawaiian geologic history. Seven G28 alleles have been identified from a sampling of 153 individuals. The G28 locus is comprised of a tri-nucleotide, imperfect motif which permits examination of the relationships between alleles and allows for detection of potential size homoplasy within the repetitive element. Alignment of G28 allele sequence data across multiple unrelated individuals suggests that alleles of like size are homologous within Hawaii. A variety of gap coding methods are explored in the inference of allele evolution. Length differences between alleles appear to be the result of polymerase slippage at multiple positions in the repetitive element suggesting an intricate process of allelic evolution which is not necessarily stepwise. Complex migration scenarios must be invoked to explain the current geographic distribution of alleles if their evolution was in fact sequential (from longest to shortest or from shortest to longest) as predicted by the “progression rule”

Keirle et al., (SUPPLEMENTARY DOCUMENTS)

Keirle et al., (SUPPLEMENTARY DOCUMENTS