129 research outputs found
Effects of extractable protein hydrolysates, lipids, and polyphenolic compounds from pearl millet (Pennisetum glaucum (L.) R. BR.) whole grain flours on starch digestibility
Pearl millet and other minor cereal production is marginalized in the Sahara of Algeria (Tidikelt and Hoggar regions). Their productions in these areas depend on traditional harvesting and processing. Pearl millet seeds are used as animal feed and rarely for human consumption. This work was to assess the starch digestion of pearl millet cultivated in the arid areas of Algeria. The seeds from this cereal could provide broad potential benefits to human health. However, their digestion properties have not been reported. Therefore, in this study, the in-vitro starch digestibility of pearl millet flour and the effect of processing on the expected glycemic index (eGI) were investigated. Grains from six pearl millet samples were chosen from two regions: Tidikelt and Hoggar. Five flours were prepared by dry milling (MF) and different treatments after dry milling such as extraction of phenolic compounds (MF-PP), lipid extraction (MF-L), protein hydrolysate extraction (MF-P) or lipid plus protein hydrolysate extraction (MF-L-P). The flours were then subjected to digestion, and the effects of grain treatments on the in vitro starch digestion were investigated. For all pearl millet samples, the kinetics of in vitro starch digestion displayed first-order model as substrates were digested to different extents; k (kinetic constant), C∞ (percentage of starch hydrolyzed at infinite time), HI (hydrolysis index) and eGI (expected glycemic index) of the samples were also calculated. Significant increases in C∞, HI and eGI (P<0.05) of the samples were observed after extraction of proteins or proteins plus lipids from flour. Four flours obtained after lipid extraction and five flours from extraction of phenolic compounds had low glycemic index (<55), with values ranging between 31.36 and 44.97. In contrast, flours obtained from protein hydrolysate extraction or lipids plus protein hydrolysates had the highest glycemic index (>69), with values ranging between 77.50 and 121.44. This study confirmed that some of the processed pearl millet seed flours have acceptable nutritional values suitable for human health and nutrition due to the low glycemic index values
Empirical equations for the estimation of natural mortality in Mediterranean teleosts
Empirical relationships were established linking estimates of the instantaneous rate of natural mortality (M), the von Bertalanffy growth parameters, L sub( infinity ) (or W sub( infinity )) and K, and annual mean water temperature in 56 stocks of Mediterranean teleosts fish. It is suggested that these relationships generate for these fish more reliable estimates of M than the widely-used model of Pauly (1980, J. Cons. CIEM 33(3):175-192), which was based on 175 fish stocks, but included only five stocks from the Mediterranean
Empirical equations for the estimation of natural mortality in Mediterranean teleosts
Natural mortality, Statistical analysis, Mediterranean Region, Teleostei
Sorafenib improves survival of FLT3-mutated acute myeloid leukemia in relapse after allogeneic stem cell transplantation: a report of EBMT acute leukemia Working Party
The Mutant Form of Lamin A that Causes Hutchinson-Gilford Progeria Is a Biomarker of Cellular Aging in Human Skin
Hutchinson-Gilford progeria syndrome (HGPS, OMIM 176670) is a rare disorder characterized by accelerated aging and early death, frequently from stroke or coronary artery disease. 90% of HGPS cases carry the LMNA G608G (GGC>GGT) mutation within exon 11 of LMNA, activating a splice donor site that results in production of a dominant negative form of lamin A protein, denoted progerin. Screening 150 skin biopsies from unaffected individuals (newborn to 97 years) showed that a similar splicing event occurs in vivo at a low level in the skin at all ages. While progerin mRNA remains low, the protein accumulates in the skin with age in a subset of dermal fibroblasts and in a few terminally differentiated keratinocytes. Progerin-positive fibroblasts localize near the basement membrane and in the papillary dermis of young adult skin; however, their numbers increase and their distribution reaches the deep reticular dermis in elderly skin. Our findings demonstrate that progerin expression is a biomarker of normal cellular aging and may potentially be linked to terminal differentiation and senescence in elderly individuals
Misguided Transcriptional Elongation Causes Mixed Lineage Leukemia
Investigation of the activity of a family of fusion proteins that cause aggressive leukemia suggests transcriptional elongation as a new mechanism for oncogenic transformation
Defective Lamin A-Rb Signaling in Hutchinson-Gilford Progeria Syndrome and Reversal by Farnesyltransferase Inhibition
Hutchinson-Gilford Progeria Syndrome (HGPS) is a rare premature aging disorder caused by a de novo heterozygous point mutation G608G (GGC>GGT) within exon 11 of LMNA gene encoding A-type nuclear lamins. This mutation elicits an internal deletion of 50 amino acids in the carboxyl-terminus of prelamin A. The truncated protein, progerin, retains a farnesylated cysteine at its carboxyl terminus, a modification involved in HGPS pathogenesis. Inhibition of protein farnesylation has been shown to improve abnormal nuclear morphology and phenotype in cellular and animal models of HGPS. We analyzed global gene expression changes in fibroblasts from human subjects with HGPS and found that a lamin A-Rb signaling network is a major defective regulatory axis. Treatment of fibroblasts with a protein farnesyltransferase inhibitor reversed the gene expression defects. Our study identifies Rb as a key factor in HGPS pathogenesis and suggests that its modulation could ameliorate premature aging and possibly complications of physiological aging
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