Spin Bottlenecks in the Quantum Hall Regim

We present a theory of time-dependent tunneling between a metal and a partially spin-polarized two-dimensional electron system (2DES). We find that the leakage current which flows to screen an electric field between the metal and the 2DES is the sum of two exponential contributions whose relative weights depend on spin-dependent tunneling conductances, on quantum corrections to the electrostatic capacitance of the tunnel junction, and on the rate at which the 2DES spin-polarization approaches equilibrium. For high-mobility and homogeneous 2DES's at Landau level filling factor $\nu=1$, we predict a ratio of the fast and slow leakage rates equal to $(2K+1)^2$ where $K$ is the number of reversed spins in the skyrmionic elementary charged excitations.Comment: 4 pages, 3 figures, submitted to Phys. Rev. Let

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly

Water Conservation for the County of San Luis Obispo

This study is an academic research project completed to satisfy the California Polytechnic Master Thesis Requirement for the Master Degree of City and Regional Planning in the College of Architecture and Environmental Design. The County of San Luis Obispo Planning and Building Department is the client for this professional project. The project was requested by the County of San Luis Obispo to assist in its water conservation efforts and to help achieve a 20 percent per capita reduction of water use by the year 2020. The project consists of two documents: A Handbook of Water Conservation Technologies and Practices (Handbook) and the Background Report for the Water Conservation Handbook (Background Report). The Handbook is intended to provide a quick guide to various water conserving fixtures and appliances that developers and residents throughout the County of San Luis Obispo can reference for personal use. The Handbook includes information on the type of technology, the benefits in terms of water saving potential, and the cost of implementation. The Handbook is organized based on Indoor and Outdoor water uses. The Background Report is a supplemental document for the Handbook which provides more in depth descriptions and examples on each technology. The Background Report also provides history on water conservation issues in California and San Luis Obispo. It looks at two case studies: one, on the water conservation efforts in Phoenix, Arizona, and two, on a plumbing retrofit project on the Cal Poly Campus that was awarded LEED certification for its water savings. Furthermore, the Background Report explores case studies in the use of community participatory planning to produce water conservation plans. The studies focused on a Community Plan development in Arroyo Grande, California, and on an Integrated Regional Water Management Plan in the Greater Los Angeles Region. The Background Report concludes by providing some cautionary advice on water conservation technologies and provides future recommendations for the county of San Luis Obispo to improve its water conservation efforts

The stochastic separatrix and the reaction coordinate for complex systems

We present a new approach to the identification of degrees of freedom which comprise a reaction coordinate in a complex system. The method begins with the generation of an ensemble of reactive trajectories. Each trajectory is analyzed for its equicommittor position or transition state; then the transition state ensemble is identified as the stochastic separatrix. Numerical analysis of the points along the separatrix for variability of coordinate location correctly identifies the components of the reaction coordinate in a test system of a double well coupled to a promoting vibration and a bath of linearly coupled oscillators

Electric Fields and Fast Protein Dynamics in Enzymes

In recent years, there has been much discussion regarding the origin of enzymatic catalysis and whether including protein dynamics is necessary for understanding catalytic enhancement. An important contribution in this debate was made with the application of the vibrational Stark effect spectroscopy to measure electric fields in the active site. This provided a window on electric fields at the transition state in enzymatic reactions. We performed computational studies on two enzymes where we have shown that fast dynamics is part of the reaction mechanism and calculated the electric field near the bond-breaking event. We found that the fast motions that we had identified lead to an increase of the electric field, thus preparing an enzymatic configuration that is electrostatically favorable for the catalytic chemical step. We also studied the enzyme that has been the subject of Stark spectroscopy, ketosteroid isomerase, and found electric fields of a similar magnitude to the two previous examples

Approximate inclusion of quantum effects in transition path sampling

We propose a method for incorporating nuclear quantum effects in transition path sampling studies of systems that consist of a few degrees of freedom that must be treated quantum mechanically, while the rest are classical-like. We used the normal mode centroid method to describe the quantum subsystem, which is a method that is not CPU intensive but still reasonably accurate. We applied this mixed centroid∕classical transition path sampling method to a model system that has nontrivial quantum behavior, and showed that it can capture the correct quantum dynamical features

Incorporating Fast Protein Dynamics into Enzyme Design: A Proposed Mutant Aromatic Amine Dehydrogenase

In recent years, there has been encouraging progress in the engineering of enzymes that are designed to catalyze reactions not accelerated by natural enzymes. We tested the possibility of reengineering an existing enzyme by introducing a fast protein motion that couples to the reaction. Aromatic amine dehydrogenase is a system that has been shown to use a fast substrate motion as part of the reaction mechanism. We identified a mutation that preserves this fast motion but also introduces a favorable fast motion near the active site that did not exist in the native enzyme. Transition path sampling was used for the analysis of the atomic details of the mechanism