8 research outputs found
Biosensing Technologies for Mycobacterium tuberculosis Detection: Status and New Developments
Biosensing technologies promise to improve Mycobacterium tuberculosis (M. tuberculosis) detection and management in clinical diagnosis, food analysis, bioprocess, and environmental monitoring. A variety of portable, rapid, and sensitive biosensors with immediate “on-the-spot” interpretation have been developed for M. tuberculosis detection based on different biological elements recognition systems and basic signal transducer principles. Here, we present a synopsis of current developments of biosensing technologies for M. tuberculosis detection, which are classified on the basis of basic signal transducer principles, including piezoelectric quartz crystal biosensors, electrochemical biosensors, and magnetoelastic biosensors. Special attention is paid to the methods for improving the framework and analytical parameters of the biosensors, including sensitivity and analysis time as well as automation of analysis procedures. Challenges and perspectives of biosensing technologies development for M. tuberculosis detection are also discussed in the final part of this paper
Fluorescent Nanoparticle-Based Indirect Immunofluorescence Microscopy for Detection of Mycobacterium tuberculosis
A method of fluorescent nanoparticle-based indirect immunofluorescence microscopy
(FNP-IIFM) was developed for the rapid detection of Mycobacterium tuberculosis.
An anti-Mycobacterium tuberculosis antibody was used as primary antibody to recognize
Mycobacterium tuberculosis, and then an antibody binding protein (Protein A) labeled with
Tris(2,2-bipyridyl)dichlororuthenium(II) hexahydrate (RuBpy)-doped silica nanoparticles was
used to generate fluorescent signal for microscopic examination. Prior to the detection, Protein A was immobilized on RuBpy-doped silica nanoparticles with a coverage of ∼5.1×102 molecules/nanoparticle. With this method, Mycobacterium tuberculosis in bacterial mixture as
well as in spiked sputum was detected. The use of the fluorescent nanoparticles reveals amplified
signal intensity and higher photostability than the direct use of conventional fluorescent dye as
label. Our preliminary studies have demonstrated the potential application of the FNP-IIFM
method for rapid detection of Mycobacterium tuberculosis in clinical samples