MiRNA-34 and stress response

Psychiatric disorders are known to result from a strong interaction between genetic predisposition and environmental factors, mainly exposure to stressful events. Environmental events can modulate genes expression, possibly via epigenetic mechanisms, and affect onset/expression of a disease [1]. Epigenetic mechanisms include, among others, post-transcriptional regulation by non-coding RNAs such as microRNAs (miRNAs). MiRNAs are small non-coding RNAs predicted to regulate hundreds of targets and to be engaged in every biological process [2]. Thanks to their ability to fine-tune gene expression, miRNAs can control gene expression patterns favoring organism’s adaptation to internal and environmental (external) factors [3], such as stressful events

Effects of lack of microRNA-34 on the neural circuitry underlying the stress response and anxiety

Stress-related psychiatric disorders, including anxiety, are complex diseases that have genetic, and environmental causes. Stressful experiences increase the release of prefrontal amygdala neurotransmitters, a response that is relevant to cognitive, emotional, and behavioral coping. Moreover, exposure to stress elicits anxiety-like behavior and dendritic remodeling in the amygdala. Members of the miR-34 family have been suggested to regulate synaptic plasticity and neurotransmission processes, which mediate stress-related disorders. Using mice that harbored targeted deletions of all 3 members of the miR-34-family (miR-34-TKO), we evaluated acute stress-induced basolateral amygdala (BLA)-GABAergic and medial prefrontal cortex (mpFC) aminergic outflow by intracerebral in vivo microdialysis. Moreover, we also examined fear conditioning/extinction, stress-induced anxiety, and dendritic remodeling in the BLA of stress-exposed TKO mice. We found that TKO mice showed resilience to stress-induced anxiety and facilitation in fear extinction. Accordingly, no significant increase was evident in aminergic prefrontal or amygdala GABA release, and no significant acute stress-induced amygdalar dendritic remodeling was observed in TKO mice. Differential GRM7, 5-HT2C, and CRFR1 mRNA expressionwas noted in the mpFC and BLA between TKO andWT mice. Our data demonstrate that the miR-34 has a critical function in regulating the behavioral and neurochemical response to acute stress and in inducing stress-related amygdala neuroplasticity

The helium spread in the Globular cluster 47 Tuc

Spectroscopy has shown the presence of the CN band dicothomy and the Na-O anticorrelations for 50--70% of the investigated samples in the cluster 47 Tuc, otherwise considered a "normal" prototype of high metallicity clusters from the photometric analysis. Very recently, the re-analysis of a large number of archival HST data of the cluster core has been able to put into evidence the presence of structures in the Sub Giant Branch: it has a brighter component with a spread in magnitude by $\sim$0.06 mag and a second one, made of about 10% of stars, a little fainter (by $\sim$0.05 mag). These data also show that the Main Sequence of the cluster has an intrinsic spread in color which, if interpreted as due to a small spread in helium abundance, suggests $\Delta$Y$\sim$0.027. In this work we examine in detail whether the Horizontal Branch morphology and the Sub Giant structure provide further independent indications that a real --although very small-helium spread is present in the cluster. We re--analyze the HST archival data for the Horizontal Branch of 47 Tuc, obtaining a sample of $\sim$500 stars with very small photometric errors, and build population synthesis based on new models to show that its particular morphology can be better explained by taking into account a spread in helium abundance of 2% in mass. The same variation in helium is able to explain the spread in luminosity of the Sub Giant Branch, while a small part of the second generation is characterized by a small C+N+O increase and provides an explanation for the fainter Sub Giant Branch. We conclude that three photometric features concur to form the paradigm that a small but real helium spread is present in a cluster that has no spectacular evidence for multiple populations like those shown by other massive clusters.Comment: Accepted for publication in the MNRAS on 2010 June 8. Received 2010 May 19; in original form 2010 February 9. 7 pages and 3 figures. No table

The rotational shear layer inside the early red-giant star KIC 4448777

We present the asteroseismic study of the early red-giant star KIC 4448777, complementing and integrating a previous work (Di Mauro et al. 2016), aimed at characterizing the dynamics of its interior by analyzing the overall set of data collected by the {\it Kepler} satellite during the four years of its first nominal mission. We adopted the Bayesian inference code DIAMOND (Corsaro \& De Ridder 2014) for the peak bagging analysis and asteroseismic splitting inversion methods to derive the internal rotational profile of the star. The detection of new splittings of mixed modes, more concentrated in the very inner part of the helium core, allowed us to reconstruct the angular velocity profile deeper into the interior of the star and to disentangle the details better than in Paper I: the helium core rotates almost rigidly about 6 times faster than the convective envelope, while part of the hydrogen shell seems to rotate at a constant velocity about 1.15 times lower than the He core. In particular, we studied the internal shear layer between the fast-rotating radiative interior and the slow convective zone and we found that it lies partially inside the hydrogen shell above $r \simeq 0.05R$ and extends across the core-envelope boundary. Finally, we theoretically explored the possibility for the future to sound the convective envelope in the red-giant stars and we concluded that the inversion of a set of splittings with only low-harmonic degree $l\leq 3$, even supposing a very large number of modes, will not allow to resolve the rotational profile of this region in detail.Comment: accepted for publication on Ap

A test for asymptotic giant branch evolution theories: Planetary Nebulae in the Large Magellanic Cloud

We used a new generation of asymptotic giant branch (AGB) stellar models that include dust formation in the stellar winds to find the links between evolutionary models and the observed properties of a homogeneous sample of Large Magellanic Cloud (LMC) planetary nebulae (PNe). Comparison between the evolutionary yields of elements such as CNO and the corresponding observed chemical abundances is a powerful tool to shed light on evolutionary processes such as hot bottom burning (HBB) and third dredge-up (TDU). We found that the occurrence of HBB is needed to interpret the nitrogen-enriched (log(N/H)+12>8) PNe. In particular, N-rich PNe with the lowest carbon content are nicely reproduced by AGB models of mass M >=6 Mo, whose surface chemistry reflects the pure effects of HBB. PNe with log(N/H)+12<7.5 correspond to ejecta of stars that have not experienced HBB, with initial mass below about 3 Mo. Some of these stars show very large carbon abundances, owing to the many TDU episodes experienced. We found from our LMC PN sample that there is a threshold to the amount of carbon accumulated at AGB surfaces, log(C/H)+12<9. Confirmation of this constraint would indicate that, after the C-star stage is reached,AGBs experience only a few thermal pulses, which suggests a rapid loss of the external mantle, probably owing to the effects of radiation pressure on carbonaceous dust particles present in the circumstellar envelope. The implications of these findings for AGB evolution theories and the need to extend the PN sample currently available are discussed.Comment: 12 pages, 4 figures, 1 table, accepted for publication in MNRAS (2015 July 13; in original form 2015 June 9

Magnesium isotopes: a tool to understand self-enrichment in Globular Clusters

A critical issue in the asymptotic giant branch (AGB) self-enrichment scenario for the formation of multiple populations in Globular Clusters (GCs) is the inability to reproduce the magnesium isotopic ratios, despite the model in principle can account for the depletion of magnesium. In this work we analyze how the uncertainties on the various p-capture cross sections affect the results related to the magnesium content of the ejecta of AGB stars. The observed distribution of the magnesium isotopes and of the overall Mg-Al trend in M13 and NGC 6752 are successfully reproduced when the proton-capture rate by 25Mg at the temperatures 100 MK, in particular the 25Mg(p, gamma)26Alm channel, is enhanced by a factor 3 with respect to the most recent experimental determinations. This assumption also allows to reproduce the full extent of the Mg spread and the Mg-Si anticorrelation observed in NGC 2419. The uncertainties in the rate of the 25Mg(p,gamma)26Alm reaction at the temperatures of interest here leave space for our assumption and we suggest that new experimental measurements are needed to settle this problem. We also discuss the competitive model based on the super massive star nucleosynthesis.Comment: accepted for publication on MNRA

Planetary Nebulae in the Small Magellanic Cloud

We analyse the planetary nebulae (PNe) population of the Small Magellanic Cloud (SMC), based on evolutionary models of stars with metallicities in the range $10^{-3} \leq Z \leq 4\times 10^{-3}$ and mass $0.9 M\odot < M < 8M\odot$, evolved through the asymptotic giant branch (AGB) phase. The models used account for dust formation in the circumstellar envelope. To characterise the PNe sample of the SMC, we compare the observed abundances of the various species with the final chemical composition of the AGB models: this study allows us to identify the progenitors of the PNe observed, in terms of mass and chemical composition. According to our interpretation, most of the PNe descend from low-mass ($M < 2 M\odot$) stars, which become carbon rich, after experiencing repeated third dredge-up episodes, during the AGB phase. A fraction of the PNe showing the signature of advanced CNO processing are interpreted as the progeny of massive AGB stars, with mass above $\sim 6 M\odot$, undergoing strong hot bottom burning. The differences with the chemical composition of the PNe population of the Large Magellanic Cloud (LMC) is explained on the basis of the diverse star formation history and age-metallicity relation of the two galaxies. The implications of the present study for some still highly debated points regarding the AGB evolution are also commented.Comment: Accepted for publication in MNRAS, 11 pages, 4 figure

Constraints on the core mu-gradient of the solar-like star HD 49385 via low-degree mixed modes

The existence of an l=1 avoided crossing in the spectrum of the solar-like pulsator CoRoT-target HD 49385 was established by Deheuvels & Michel (2009). It is the first confirmed detection of such a phenomenon. The authors showed in a preliminary modeling of the star that it was in a post main sequence status. Being a 1.3 Msun-star, HD 49385 has had a convective core during its main sequence phase. The mu-gradient left by the withdrawal of this core bears information about the processes of transport at the boundary of the core. We here investigate the constraints that the observed avoided crossing brings on the mu-gradient in the core of the star.Comment: 5 pages, 5 figures, accepted in Astron. Nach. This work was presented at the HELAS Conference in Lanzarote in February 201