Leaders\u2019 competence and warmth: Their relationships with employees\u2019 well-being and organizational effectiveness

The aim of this work was to investigate competence and warmth \u2014 the two basic dimensions of social judgment \u2014 as dimensions employees use to evaluate their supervisors. A mediation model was tested in which supervisor\u2019s perceived competence and warmth were associated with relevant outcomes (lower burnout, weaker turnover intentions, more frequent citizenship behaviors) through the mediation of affective organizational commitment (AOC). In Study 1, data were collected from employees of a company in the water service sector. In Study 2, participants were financial promoters. In Study 3, the sample included employees from different organizations. As hypothesized, the perception of one\u2019s supervisor as competent (Studies 1-3) and warm (Study 3) was related to employees\u2019 lower burnout, weaker turnover intentions, more frequent prosocial behaviors through the mediation of AOC. Theoretical and practical implications of findings are discussed

Priming attachment security and outgroup humanization: The mediation role of intergroup emotions

Individuals tend to dehumanize the outgroup. In this paper, we explore whether the activation of attachment security can attenuate dehumanization. Two studies were performed. In Study 1, attachment security was primed by showing pictures that depicted relationships with attachment figures; the outgroup was the homeless and humanization was measured considering the attribution of uniquely human and non-uniquely human emotions to this group. In Study 2, the sense of interpersonal security was activated by inviting participants to relive a recent interaction that left them with a feeling of safety and warmth. Outgroup members were the Roma, and humanization was measured considering the attribution of uniquely human and human nature traits to them. In Study 2, the mediation effect of intergroup emotions was investigated. In both studies, outgroup humanization effects were highlighted. In Study 2, these effects were mediated by increased empathy toward the outgroup. Interestingly, the positive impact of security activation was not moderated by chronic attachment orientations. Findings suggest strategies that can be used to improve intergroup relations in specific contexts and in society at large

Automatic synchronisation of the cell cycle in budding yeast through closed-loop feedback control

The cell cycle is the process by which eukaryotic cells replicate. Yeast cells cycle asynchronously with each cell in the population budding at a different time. Although there are several experimental approaches to synchronise cells, these usually work only in the short-term. Here, we build a cyber-genetic system to achieve long-term synchronisation of the cell population, by interfacing genetically modified yeast cells with a computer by means of microfluidics to dynamically change medium, and a microscope to estimate cell cycle phases of individual cells. The computer implements a controller algorithm to decide when, and for how long, to change the growth medium to synchronise the cell-cycle across the population. Our work builds upon solid theoretical foundations provided by Control Engineering. In addition to providing an avenue for yeast cell cycle synchronisation, our work shows that control engineering can be used to automatically steer complex biological processes towards desired behaviours similarly to what is currently done with robots and autonomous vehicles

A single-cell analysis of breast cancer cell lines to study tumour heterogeneity and drug response

Cancer cells within a tumour have heterogeneous phenotypes and exhibit dynamic plasticity. How to evaluate such heterogeneity and its impact on outcome and drug response is still unclear. Here, we transcriptionally profile 35,276 individual cells from 32 breast cancer cell lines to yield a single cell atlas. We find high degree of heterogeneity in the expression of biomarkers. We then train a deconvolution algorithm on the atlas to determine cell line composition from bulk gene expression profiles of tumour biopsies, thus enabling cell line-based patient stratification. Finally, we link results from large-scale in vitro drug screening in cell lines to the single cell data to computationally predict drug responses starting from single-cell profiles. We find that transcriptional heterogeneity enables cells with differential drug sensitivity to co-exist in the same population. Our work provides a framework to determine tumour heterogeneity in terms of cell line composition and drug response

Parental Support during the COVID-19 Pandemic: Friend or Foe? A Moderation Analysis of the Association between Maternal Anxiety and Children’s Stress in Italian Dyads

There is evidence that parental psychological disorders in stressful situations increase the risk of disturbance in child development. This has been investigated in disasters but not in pandemics, which are sensibly different from other types of traumatic events. We investigated the relationship between mothers’ anxiety and their children’s (self-reported) stress and the boundary conditions of this association during the first full COVID-19 lockdown in Italy. During the COVID-19 pandemic, mothers might have increased their protective attitudes to secure and support their children; we tested whether the relationship between mothers’ anxiety and children’s stress was weaker (buffer effect) or stronger (over-protection effect) when perceived parental support was high. We measured mothers’ anxiety, children’s perceived parental support, and children’s stress in a sample of 414 8- to 11-year-old primary school children (229 females, Mage = 9.44) and 395 mothers (Mage = 42.84). Results supported the over-protection scenario and provided the first evidence for the “helicopter-parent effect” during the COVID-19 pandemic: mothers’ anxiety was positively associated with children’s stress only when perceived support was high. Our finding highlights the importance of educating parents (for example, via emotional training) to prevent the worst consequences of adverse events in children and promote their mental health

Body Shame in 7–12-Year-Old Girls and Boys: The Role of Parental Attention to Children’s Appearance

Guided by the Tripartite Influence Model and Objectification Theory, we examined whether parents’ attention to their children’s appearance was related to higher body shame in girls and boys. In Study 1 (N = 195) and 2 (N = 163), we investigated 7-12-year-old children’s metaperceptions about parents’ attention to their appearance and its association with children’s body shame. In Study 3, we examined the link between parents’ self-reported attention to their children’s appearance and children’s body shame among parent-child triads (N = 70). Results demonstrated that both children’s metaperceptions and fathers’ self-reported attention to children’s appearance were associated with body shame in children. Furthermore, when mothers’ and fathers’ attitudes toward their children were analyzed simultaneously, only fathers’ attention to their children’s appearance was associated with greater body shame in girls and boys. Notably, no gender differences emerged, suggesting that parents’ attention to their children’s appearance was not differentially related to body shame in girls and boys. These results remained significant when controlling for other sources of influence, namely peer and media influence, both of which were found to have a strong association with body shame in children. Theoretical and practical implications of our findings are discussed

Cosmic-ray propagation with DRAGON2: I. numerical solver and astrophysical ingredients

We present version 2 of the DRAGON code designed for computing realistic predictions of the CR densities in the Galaxy. The code numerically solves the interstellar CR transport equation (including inhomogeneous and anisotropic diffusion, either in space and momentum, advective transport and energy losses), under realistic conditions. The new version includes an updated numerical solver and several models for the astrophysical ingredients involved in the transport equation. Improvements in the accuracy of the numerical solution are proved against analytical solutions and in reference diffusion scenarios. The novel features implemented in the code allow to simulate the diverse scenarios proposed to reproduce the most recent measurements of local and diffuse CR fluxes, going beyond the limitations of the homogeneous galactic transport paradigm. To this end, several applications using DRAGON2 are presented as well. This new version facilitates the users to include their own physical models by means of a modular C++ structure. © 2017 IOP Publishing Ltd and Sissa Medialab srl

The impact of microRNAs on transcriptional heterogeneity and gene co-expression across single embryonic stem cells

MicroRNAs act posttranscriptionally to suppress multiple target genes within a cell population. To what extent this multi-target suppression occurs in individual cells and how it impacts transcriptional heterogeneity and gene co-expression remains unknown. Here we used single-cell sequencing combined with introduction of individual microRNAs. miR-294 and let-7c were introduced into otherwise microRNA-deficient Dgcr8 knockout mouse embryonic stem cells. Both microRNAs induce suppression and correlated expression of their respective gene targets. The two microRNAs had opposing effects on transcriptional heterogeneity within the cell population, with let-7c increasing and miR-294 decreasing the heterogeneity between cells. Furthermore, let-7c promotes, whereas miR-294 suppresses, the phasing of cell cycle genes. These results show at the individual cell level how a microRNA simultaneously has impacts on its many targets and how that in turn can influence a population of cells. The findings have important implications in the understanding of how microRNAs influence the co-expression of genes and pathways, and thus ultimately cell fate

High-intensity ultrashort laser-induced ablation of stainless steel foil targets in the presence of ambient gas

Ablation depths of stainless steel targets irradiated by 80-fs laser pulses at a flux F ≤ 40 J/cm2 (intensity ≤ 5 × 1014 W/cm2) in the presence of air at atmospheric pressure are experimentally measured. These values are lower than the theoretical predictions for metal targets in vacuum. Results are analyzed on the basis of the role of the ambient gas and of crater formation on the behavior of the ablated material