Aphid Performance Changes with Plant Defense Mediated by \u3cem\u3eCucumber mosaic virus\u3c/em\u3e Titer

Background: Cucumber mosaic virus (CMV) causes appreciable losses in vegetables, ornamentals and agricultural crops. The green peach aphid, Myzus persicae Sulzer (Aphididae) is one of the most efficient vectors for CMV. The transmission ecology of aphid-vectored CMV has been well investigated. However, the detailed description of the dynamic change in the plant-CMV-aphid interaction associated with plant defense and virus epidemics is not well known. Results: In this report, we investigated the relationship of virus titer with plant defense of salicylic acid (SA) and jasmonic acid (JA) during the different infection time and their interaction with aphids in CMV-infected tobacco plants. Our results showed that aphid performance changed with virus titer and plant defense on CMV-inoculated plants. At first, plant defense was low and aphid number increased gradually. The plant defense of SA signaling pathway was induced when virus titer was at a high level, and aphid performance was correspondingly reduced. Additionally, the winged aphids were increased. Conclusion: Our results showed that aphid performance was reduced due to the induced plant defense mediated by Cucumber mosaic virus titer. Additionally, some wingless aphids became to winged aphids. In this way CMV could be transmitted with the migration of winged aphids. We should take measures to prevent aphids in the early stage of their occurrence in the field to prevent virus outbreak

Effect of Science and Technology Finance Policy on Urban Green Development in China

Green sustainable development is a major challenge faced by countries worldwide. Against the strategic background of innovation-driven development, studying the impact of science and technology finance policy (STFP) on urban green development is of great practical significance. Based on urban panel data from 2003 to 2019, this study systematically examines whether and how STFP affects urban green development in China using the difference-in-differences (DID) method. The empirical results show that STFP has significantly stimulated the urban green development level, and the effect of policy implementation has increased first and then decreased over time. The findings remain robust when using propensity score matching DID to avoid selection bias and other factors that may interfere with the estimation results. Additionally, technological innovation and green innovation are essential channels for STFP to improve urban green development. The impact of STFP is found to vary by region and by the level of urban innovation. Specifically, the policy effect is more pronounced in midwestern and high-innovation cities but less obvious in eastern and low-innovation cities. In conclusion, this study provides city-level empirical evidence from China for an in-depth understanding of the green economy effect of STFP. It also provides theoretical guidance and policy references for accelerating the green transition in the context of sustainable development

The genome evolution and domestication of tropical fruit mango

Background: Mango is one of the world’s most important tropical fruits. It belongs to the family Anacardiaceae, which includes several other economically important species, notably cashew, sumac and pistachio from other genera. Many species in this family produce family-specific urushiols and related phenols, which can induce contact dermatitis. Results: We generate a chromosome-scale genome assembly of mango, providing a reference genome for the Anacardiaceae family. Our results indicate the occurrence of a recent whole-genome duplication (WGD) event in mango. Duplicated genes preferentially retained include photosynthetic, photorespiration, and lipid metabolic genes that may have provided adaptive advantages to sharp historical decreases in atmospheric carbon dioxide and global temperatures. A notable example of an extended gene family is the chalcone synthase (CHS) family of genes, and particular genes in this family show universally higher expression in peels than in flesh, likely for the biosynthesis of urushiols and related phenols. Genome resequencing reveals two distinct groups of mango varieties, with commercial varieties clustered with India germplasms and demonstrating allelic admixture, and indigenous varieties from Southeast Asia in the second group. Landraces indigenous in China formed distinct clades, and some showed admixture in genomes. Conclusions: Analysis of chromosome-scale mango genome sequences reveals photosynthesis and lipid metabolism are preferentially retained after a recent WGD event, and expansion of CHS genes is likely associated with urushiol biosynthesis in mango. Genome resequencing clarifies two groups of mango varieties, discovers allelic admixture in commercial varieties, and shows distinct genetic background of landraces

Tomato Chlorosis Virus Infection Facilitates \u3cem\u3eBemisia tabaci\u3c/em\u3e MED Reproduction by Elevating \u3cem\u3eVitellogenin\u3c/em\u3e Expression

Transmission of plant pathogenic viruses mostly relies on insect vectors. Plant virus could enhance its transmission by modulating the vector. Previously, we showed that feeding on virus infected plants can promote the reproduction of the sweet potato whitefly, Bemisia tabaci MED (Q biotype). In this study, using a whitefly-Tomato chlorosis virus (ToCV)-tomato system, we investigated how ToCV modulates B. tabaci MED reproduction to facilitate its spread. Here, we hypothesized that ToCV-infected tomato plants would increase B. tabaci MED fecundity via elevated vitellogenin (Vg) gene expression. As a result, fecundity and the relative expression of B. tabaci MED Vg was measured on ToCV-infected and uninfected tomato plants on days 4, 8, 12, 16, 20 and 24. The role of Vg on B. tabaci MED reproduction was examined in the presence and absence of ToCV using dietary RNAi. ToCV infection significantly increased B. tabaci MED fecundity on days 12, 16 and 20, and elevated Vg expression on days 8, 12 and 16. Both ovarian development and fecundity of B. tabaci MED were suppressed when Vg was silenced with or without ToCV infection. These combined results suggest that ToCV infection increases B. tabaci MED fecundity via elevated Vg expression

A Review of Spatter in Laser Powder Bed Fusion Additive Manufacturing: In Situ Detection, Generation, Effects, and Countermeasures

Spatter is an inherent, unpreventable, and undesired phenomenon in laser powder bed fusion (L-PBF) additive manufacturing. Spatter behavior has an intrinsic correlation with the forming quality in L-PBF because it leads to metallurgical defects and the degradation of mechanical properties. This impact becomes more severe in the fabrication of large-sized parts during the multi-laser L-PBF process. Therefore, investigations of spatter generation and countermeasures have become more urgent. Although much research has provided insights into the melt pool, microstructure, and mechanical property, reviews of spatter in L-PBF are still limited. This work reviews the literature on the in situ detection, generation, effects, and countermeasures of spatter in L-PBF. It is expected to pave the way towards a novel generation of highly efficient and intelligent L-PBF systems

The genome evolution and domestication of tropical fruit mango

Background: Mango is one of the world’s most important tropical fruits. It belongs to the family Anacardiaceae, which includes several other economically important species, notably cashew, sumac and pistachio from other genera. Many species in this family produce family-specific urushiols and related phenols, which can induce contact dermatitis. Results: We generate a chromosome-scale genome assembly of mango, providing a reference genome for the Anacardiaceae family. Our results indicate the occurrence of a recent whole-genome duplication (WGD) event in mango. Duplicated genes preferentially retained include photosynthetic, photorespiration, and lipid metabolic genes that may have provided adaptive advantages to sharp historical decreases in atmospheric carbon dioxide and global temperatures. A notable example of an extended gene family is the chalcone synthase (CHS) family of genes, and particular genes in this family show universally higher expression in peels than in flesh, likely for the biosynthesis of urushiols and related phenols. Genome resequencing reveals two distinct groups of mango varieties, with commercial varieties clustered with India germplasms and demonstrating allelic admixture, and indigenous varieties from Southeast Asia in the second group. Landraces indigenous in China formed distinct clades, and some showed admixture in genomes. Conclusions: Analysis of chromosome-scale mango genome sequences reveals photosynthesis and lipid metabolism are preferentially retained after a recent WGD event, and expansion of CHS genes is likely associated with urushiol biosynthesis in mango. Genome resequencing clarifies two groups of mango varieties, discovers allelic admixture in commercial varieties, and shows distinct genetic background of landraces

Monitoring Water Transparency in Shallow and Eutrophic Lake Waters Based on GOCI Observations

Water transparency represented by the Secchi disk depth (Zsd) plays an important role in understanding water ecology environment variations, especially for optically complex and shallow lake waters. In this study, using in situ measured remote sensing reflectance (Rrs), diffuse attenuation coefficient (Kd), and Zsd data collected in Lake Taihu (China), a regional algorithm for estimating Kd from Rrs was designed, and the semi-analytical model proposed by Lee et al. (2015) (hereafter called Lee_2015 model) was refined using a linear scaling correction for remote sensing of Zsd. The results showed that a good agreement between the derived Kd and in situ measured data (mean absolute percentage error (MAPE) = 26% for Kd(490); MAPE < 5% for Kd at 443, 555, and 660 nm). The in situ Rrs-derived Zsd results using the refined Lee_2015 model compared well with the in situ measured Zsd (R2 = 0.72 and MAPE = 36%), which was an obvious improvement over the Lee_2015 model in our study region. Subsequently, the refined Lee_2015 model was applied to the geostationary ocean color imager (GOCI) observations between 2012 and 2018 to yield the spatial and temporal variations of water transparency in the Lake Taihu waters. The long-term mean distribution of Zsd revealed that water transparency values in the northeastern Lake Taihu were generally higher than those in the southwest part. Monthly climatological Zsd patterns suggested that the Zsd distributions had large temporal variability, and distinct monthly patterns of Zsd existed in different subregions of Lake Taihu. The significant interannual variations of Zsd in Lake Taihu are probably affected by a combination of the water column stability mainly caused by wind, water temperature, human activity, and riverine discharge. The present study can provide a new approach for quantifying water visibility and serve for water-color remote sensing of optically complex and highly turbid waters