Development of a Biomedical Innovation Economy-Panama

In this study we consider the progress toward the development of innovation incubators within the Central American country of Panama. We attempt to identify the extent to which Panama, as a developing country with a small market size, has recognized the importance of technology and innovation in its efforts to build a sustainable economy. We examined the “Triple Helix” government-academia-industry model (Etzkowitz, 2008), and applied it to the bioscience sector, while also incorporating the role of foreign collaborators. By reinforcing and maintaining synergies among all stakeholders, while also concentrating resources in the areas of strengths and increasing R&D expenditures, Panama could become a key regional player in the bioscience sector. Examples of cooperative research innovation are highlighted, and suggestions are made to enhance their commercialization potential.In this study we consider the progress toward the development of innovation incubators within the Central American country of Panama. We attempt to identify the extent to which Panama, as a developing country with a small market size, has recognized the importance of technology and innovation in its efforts to build a sustainable economy. We examined the “Triple Helix” government-academia-industry model (Etzkowitz, 2008), and applied it to the bioscience sector, while also incorporating the role of foreign collaborators. By reinforcing and maintaining synergies among all stakeholders, while also concentrating resources in the areas of strengths and increasing R&D expenditures, Panama could become a key regional player in the bioscience sector. Examples of cooperative research innovation are highlighted, and suggestions are made to enhance their commercialization potential.In this study we consider the progress toward the development of innovation incubators within the Central American country of Panama. We attempt to identify the extent to which Panama, as a developing country with a small market size, has recognized the importance of technology and innovation in its efforts to build a sustainable economy. We examined the “Triple Helix” government-academia-industry model (Etzkowitz, 2008), and applied it to the bioscience sector, while also incorporating the role of foreign collaborators. By reinforcing and maintaining synergies among all stakeholders, while also concentrating resources in the areas of strengths and increasing R&D expenditures, Panama could become a key regional player in the bioscience sector. Examples of cooperative research innovation are highlighted, and suggestions are made to enhance their commercialization potential

Formin1 disruption confers oligodactylism and alters Bmp signaling

Proper limb development requires concerted communication between cells within the developing limb bud. Several molecules have been identified which contribute to the formation of a circuitry loop consisting in large part of secreted proteins. The intracellular actin nucleator, Formin 1 (Fmn1), has previously been implicated in limb development, but questions remain after the identification of a Gremlin transcriptional enhancer within the 3′ end of the Fmn 1 locus. To resolve this issue, a knockout mouse devoid of Fmn1 protein was created and characterized. The mice exhibit a reduction of digit number to four, a deformed posterior metatarsal, phalangeal soft tissue fusion as well as the absence of a fibula to 100% penetrance in the FVB genetic background. Importantly, this mutant allele does not genetically disrupt the characterized Gremlin enhancer, and indeed Gremlin RNA expression is upregulated at the 35 somite stage of development. Our data reveal increased Bone Morphogenetic Protein (Bmp) activity in mice which carry a disruption in Fmn1, as evidenced by upregulation of Msx1 and a decrease in Fgf4 within the apical ectodermal ridge. Additionally, these studies show enhanced activity downstream of the Bmp receptor in cells where Fmn1 is perturbed, suggesting a role for Fmn1 in repression of Bmp signalin

Turning the Page to Year 2016

As we conclude another year (2015), Volume 2 completed, we are pleased with the number of quality published manuscripts. We are also excited to announce Nanobiomedicine has been indexed in DOAJ (Directory of Open Access Journals) (https://doaj.org/toc/1849-5435)! This was in part attributed with the help of our Special Editor, Dr. Barbara Smith, who spearheaded manuscripts highlighting innovative results that impacted the global health spectrum implementing new methods for disease diagnosis, including technological and product development for enhanced point-of-care and personalized health care. Dr. Smith undertook this endeavor as she transitioned from a post-doc position (from George Whitesides’ lab at Harvard University) to a faculty position at Arizona State, getting acclimated and setting up her laboratory. We want to thank Dr. Smith for her time and commitment to our journal. It’s worth noting, we had a high number of submissions throughout the year, however, the expectations of the manuscripts not published fell short due to our review process, indicating the emphasis of publishing high quality manuscripts. We thank all the reviewers for their time and feedback

An analytical framework to assess the governance of universities and their involvement in Smart Specialisation Strategies

The renewed EU agenda for higher education (European Commission, 2017) has emphasized that higher education institutions are not contributing as much as they should to innovation in their regions and countries. The engagement of universities in S3 has shown to be particularly important in countries and regions with weaker regional innovation systems and sub-critical public institutional capacity. The ability of universities to bring together education, research and innovation, places them as particularly important stakeholders to contribute to the research and innovation system. Nevertheless, becoming more engaged in regional innovation policies and S3 requires an important strategic vision and institutional change by HEIs to be able to engage in co-creation dynamics with quadruple helix actors. Moreover, the ability of universities to adjust their working agenda could require some change in their common practices. How they can manage this, mandates a governance framework which can allow for agility from institutes steeped in tradition. The issue of governance is complex, multi-dimensional, and often involves changes in policy, behaviour and outreach for a successful implementation of set objectives. Institutional governance in general and for universities in particular, implies setting in motion or overseeing various institutional processes and regulatory provisions to allow for the planned targets and outcomes to be achieved. The current report proposes an analytical framework for university governance allowing the comparison and benchmarking of governance systems across EU member states, which could serve as guidance for university managers and policy makers to design the institutional incentives and funding programmes for increased engagement in S3. This analytical framework is experimented through a survey involving 74 European universities, the analysis of country annual reports of the Research and innovation observatory (RIO) and the knowledge generated in S2E project covering particularly EU13 countries and the higher education for Smart specialisation initiative (HESS). The main results and limits are commented and discussed with some recommendations.JRC.B.3-Territorial Developmen

Lentiviral gene transfer into the dorsal root ganglion of adult rats

<p>Abstract</p> <p>Background</p> <p>Lentivector-mediated gene delivery into the dorsal root ganglion (DRG) is a promising method for exploring pain pathophysiology and for genetic treatment of chronic neuropathic pain. In this study, a series of modified lentivector particles with different cellular promoters, envelope glycoproteins, and viral accessory proteins were generated to evaluate the requirements for efficient transduction into neuronal cells <it>in vitro </it>and adult rat DRG <it>in vivo</it>.</p> <p>Results</p> <p><it>In vitro</it>, lentivectors expressing enhanced green fluorescent protein (EGFP) under control of the human elongation factor 1α (EF1α) promoter and pseudotyped with the conventional vesicular stomatitis virus G protein (VSV-G) envelope exhibited the best performance in the transfer of EGFP into an immortalized DRG sensory neuron cell line at low multiplicities of infection (MOIs), and into primary cultured DRG neurons at higher MOIs. <it>In vivo</it>, injection of either first or second-generation EF1α-EGFP lentivectors directly into adult rat DRGs led to transduction rates of 19 ± 9% and 20 ± 8% EGFP-positive DRG neurons, respectively, detected at 4 weeks post injection. Transduced cells included a full range of neuronal phenotypes, including myelinated neurons as well as both non-peptidergic and peptidergic nociceptive unmyelinated neurons.</p> <p>Conclusion</p> <p>VSV-G pseudotyped lentivectors containing the human elongation factor 1α (EF1α)-EGFP expression cassette demonstrated relatively efficient transduction to sensory neurons following direct injection into the DRG. These results clearly show the potential of lentivectors as a viable system for delivering target genes into DRGs to explore basic mechanisms of neuropathic pain, with the potential for future clinical use in treating chronic pain.</p

Science and technology parks as innovation intermediaries for green innovation

Author's accepted version (postprint).This is an Accepted Manuscript of an article published by Springer in Lecture Notes in Mechanical Engineering on 18/08/2020.Available online: https://link.springer.com/chapter/10.1007/978-3-030-48021-9_101This paper discusses how science and technology parks (STPs) act as intermediaries for projects regarding green innovation. The empirical evidence is gathered through a case study of the City of Knowledge in Panama. For the recent Panama channel’s expansion, local authorities faced the need to improve the water resource management to secure enough fresh water for the canal’s operation. We inductively analysed data from 24 interviews, documents and participant observer. Preliminary results show the intermediation of STPs in green innovation processes in three phases: a first intermediation process is the STP as a hub for knowledge generation, including training for entrepreneurship. A second stage of the park as an innovation intermediary regards to an arena for knowledge and technology transfer, including collaboration with universities. A third phase implies financing and brokerage of green innovation between local and global actors. Our results challenge the existing literature about STPs with a narrow focus on economic spillover effects, or as hubs for attracting and developing cutting-edge technological innovations.acceptedVersio

Isoflavone glycosides: Synthesis and evaluation as α-glucosidase inhibitors

On the basis of the structure of 4′,7,8-trihydroxyisoflavone 7-O-α-D-arabinofuranoside (namely A-76202, 1), a Rhodococcus metabolite showing potent inhibitory activities against the α-glucosidases of rat liver microsome (IC 50 = 0.46 ng/mL), 26 analogs, each with minor variations at the sugar moiety and the isoflavone A and B rings, were readily synthesized. Notably, a new and efficient method was developed for the divergent synthesis of the B-ring congeners of the isoflavone glycosides by using Suzuki-Miyaura coupling as the final step. Modifications at the sugar moiety and the isoflavone A ring significantly diminish the activity, whereas variations at the B ring are largely tolerated for retaining the potent α-glucosidase inhibitory activity. © Wiley-VCH Verlag GmbH & Co. KGaA, 2008.postprin

Formin 2 links neuropsychiatric phenotypes at young age to an increased risk for dementia

Age-associated memory decline is due to variable combinations of genetic and environmental risk factors. How these risk factors interact to drive disease onset is currently unknown. Here we begin to elucidate the mechanisms by which post-traumatic stress disorder (PTSD) at a young age contributes to an increased risk to develop dementia at old age. We show that the actin nucleator Formin 2 (Fmn2) is deregulated in PTSD and in Alzheimer's disease (AD) patients. Young mice lacking the Fmn2 gene exhibit PTSD-like phenotypes and corresponding impairments of synaptic plasticity, while the consolidation of new memories is unaffected. However, Fmn2 mutant mice develop accelerated age-associated memory decline that is further increased in the presence of additional risk factors and is mechanistically linked to a loss of transcriptional homeostasis. In conclusion, our data present a new approach to explore the connection between AD risk factors across life span and provide mechanistic insight to the processes by which neuropsychiatric diseases at a young age affect the risk for developing dementia

Formin1 Mediates the Induction of Dendritogenesis and Synaptogenesis by Neurogenin3 in Mouse Hippocampal Neurons

Neurogenin3, a proneural transcription factor controlled by Notch receptor, has been recently shown to regulate dendritogenesis and synaptogenesis in mouse hippocampal neurons. However, little is known about the molecular mechanisms involved in these actions of Ngn3. We have used a microarray analysis to identify Ngn3 regulated genes related with cytoskeleton dynamics. One of such genes is Fmn1, whose protein, Formin1, is associated with actin and microtubule cytoskeleton. Overexpression of the Fmn1 isoform-Ib in cultured mouse hippocampal neurons induced an increase in the number of primary dendrites and in the number of glutamatergic synaptic inputs at 4 days in vitro. The same changes were provoked by overexpression of Ngn3. In addition downregulation of Fmn1 by the use of Fmn1-siRNAs impaired such morphological and synaptic changes induced by Ngn3 overexpression in neurons. These results reveal a previously unknown involvement of Formin1 in dendritogenesis and synaptogenesis and indicate that this protein is a key component of the Ngn3 signaling pathway that controls neuronal differentiation