Chronic constipation due to presacral teratoma in a 36-year-old woman: a case report

Abstract Introduction Teratomas of the sacrococcygeal area are usually diagnosed in infancy and are rarely seen in adults. Case presentation We report the case of a 36-year-old Greek woman experiencing chronic constipation due to a benign presacral teratoma. Imaging examinations showed a pelvic mass without evidence of malignancy. An ovoid tumour with a maximum dimension of 6 cm was surgically removed. A histologic examination revealed a mature cystic teratoma. Two years after surgery, the patient is well, with no evidence of recurrence and no constipation. Conclusion Sacrococcygeal teratomas are rare in adults. A high index of suspicion is important in making an early diagnosis. Rectal examination and radiologic evaluation are also valuable.</p

Sample parameters affecting the clinical relevance of RNA biomarkers in translational breast cancer research

In the frame of translational breast cancer research, eligibility criteria for formalin-fixed paraffin-embedded tissue (FFPE) material processing for gene expression studies include tumor cell content (TCC) and sample site (primary vs metastatic tumors). Herein we asked whether the observed differences in gene expression between paired samples with respect to TCC and sample site also have different clinical significance. We assessed ESR1, ERBB2, MAPT, MMP7, and RACGAP1 mRNA expression with real time PCR in paired samples before (NMD) and after macrodissection (MD) from 98 primary tumors (P(MD), P(NMD)) and 72 metastatic lymph nodes (LN(MD), LN(NMD)), as well as from 93 matched P (mP) and LN (mLN). TCC range was 2.5–75 % in the NMD series and 28–98 % in the MD and in the mP/mLN series. The prognostic effect of these markers, individually or in clusters, remained stable between paired P(MD/NMD). In comparison, cluster classification failed in the LN(NMD) group with lower TCC. In the mP/mLN cohort, RACGAP1 mRNA expression was of prognostic significance when tested in mLN samples (p < 0.001). Similarly, luminal B, HER2, and triple negative tumors were of dismal prognosis when classified in the LN component of the same series (mLN, overall survival: p = 0.013, p = 0.034, and p = 0.007, respectively). In conclusion, the clinical relevance of the RNA markers examined may be affected by TCC in metastatic LN samples but not in primary tumors, while it differs between primary tumors and matched metastases. These data will facilitate the design of translational studies involving FFPE sample series. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00428-012-1357-1) contains supplementary material, which is available to authorized users

THE MARINE ENVIRONMENT AND ITS INFLUENCE ON SEAFARING AND MARITIME ROUTES IN THE PREHISTORIC AEGEAN

This article seeks to illustrate the decisive contribution of the sea environment and particularly the sea-surface circulation (which is determined by the circulation of sea currents and the winds) to the early growth of seafaring and maritime communications in the prehistoric Aegean. Given the means and techniques of navigation in that era, an attempt is made to reveal a dense network of sea routes which vessels could follow through the Aegean, in order to facilitate their trip. These sea routes are primarily based on environmental data and are confirmed concurrently by archaeological evidence and data of ancient sources. Therefore, it is evident that these sea routes played an important role not only in the early inhabitation of the Aegean islands and the foundation of coastal settlements throughout the Aegean area from the sixth millennium BC to the end of the third millennium BC, but in the development of the Aegean civilization during earlier prehistoric times as well