Antimicrobial activity of the marine actinomycetes

Antibacterial activity of 107 marine actinomycetes isolated form near sea shore sediment and seawater from Konkan   coast of Maharashtra was studied. A total 107 actinomycetes were subjected to primary screening by perpendicular streak method against various test microorganisms. Among 107 actinomycetes 22,14,34,14,07,52,27 and 6 number of actinomycetal  isolates were antagonistic against Bacillus  subtilis, Staphylococcus aureus, Proteus vulgaris ,Escherichia  coli, Klebsiella aerogenes , Pseudomonas  aeruginosa.,Candida albicans  and  Aspergillus. niger respectively. Out of 107 actinomycetal isolates 13 isolates showing maximum antagonistic activities that were subjected for the secondary screening by agar well method. Finally 0 5 isolates were selected for further study on the basis of maximum zone of inhibition and broad spectrum activity. Selected 05 isolates were inoculated in glucose soybean broth 7 days at 300C and antibacterial substances were extracted with ethyl acetate.T L C of the ethyl acetate extract was carried out using N butanol : acetic acid : water  ( 4:1:5 ) as a solvent system. Spots were observed under UV light and in iodine chamber. Bioautograhy of ethyl acetate extract of selected 05 isolates were carried using test organisms B. subtilus and P. vulgaris. Inhibition zones were observed and they were associated with the purple spots at the chromatograms as detected under UV light. This may indicate the same compound was responsible for the antibacterial activity of the actinomycetes.Finaly one potent   actinomycetal isolate (GA-22) was selected and it’s morphological, cultural, physiological and biochemical characters was studied.   It was found that biochemically GA-22 was very active marine actinomycetes it was able to produce variety of enzymes and utilize number of sugars

Privacy Preserving Public Auditing and Data Integrity for Secure Cloud Storage Using Third Party Auditor

Using cloud services, anyone can remotely store their data and can have the on-demand high quality applications and services from a shared pool of computing resources, without the burden of local data storage and maintenance. Cloud is a commonplace for storing data as well as sharing of that data. However, preserving the privacy and maintaining integrity of data during public auditing remains to be an open challenge. In this paper, we introducing a third party auditor (TPA), which will keep track of all the files along with their integrity. The task of TPA is to verify the data, so that the user will be worry-free. Verification of data is done on the aggregate authenticators sent by the user and Cloud Service Provider (CSP). For this, we propose a secure cloud storage system which supports privacy-preserving public auditing and blockless data verification over the cloud

Comparative study of oxytetracycline and doxycycline on calcium chelation: in-vitro assay

Background: Tetracycline class of antibiotics differ in their pharmacokinetic profile and chelating property. Objective of present study is to assess the effect of oxytetracycline and doxycycline on calcium chelationMethods: For estimation of calcium chelation of Oxytetracycline and Doxycycline, EDTA method (P. Trinder) and calcium binding assay was followed. Different doses of Oxytetracycline (25 mg, 50 mg and 100 mg) and Doxycycline (25 mg, 50 mg and 100 mg) were used in EDTA method and different concentrations of calcium were used in calcium binding assay. The procedure was done according to the standard methodology.Results: The intensity of colour appear to be increased with increase in dose of the Oxytetracycline (25 mg, 50 mg, 100 mg) as the concentration of calcium binding increases. But in Doxycycline intensity of colour is more with 100 mg as compared with 25 mg and 50 mg The UV absorption spectrum of solution of Oxytetracycline (1mM) was changed after the addition of CaCl2 to provide different concentration of Ca2+ (0.1, 0.5 and 1.0 mM).  With minor shift in the absorption coefficient and no shift in wavelength were observed for Doxycycline.Conclusions: The study concludes that oxytetracycline has more calcium chelating property than doxycycline

Assessment of medication adherence among hypertensive patients: a cross-sectional study

Background: Hypertension affects around one billion individuals worldwide and is expected to increase by 29% to reach 1.56 billion by 2025. It is usually asymptomatic, chronic disorder needing lifelong treatment. The objective of this study was to study the medication adherence among hypertensive patients using hill-bone compliance to high blood pressure therapy scale (HILL-BONE CHBPTS) and to compare medication adherence in hypertensive patients with controlled and uncontrolled blood pressure.Methods: A cross-sectional, observational study was conducted for a period of one year in the Outpatient department of Medicine in a tertiary care hospital, Navi Mumbai. A total of 129 hypertensive patients who were on at least six months on antihypertensive medications were enrolled. Blood pressure was measured and details of drug therapy were noted. Medication adherence was assessed using HILL-BONE CHBPTS and respective scores were calculated.Results: HILL-BONE CHBPTS scores were on the higher side signifying poor medication adherence among hypertensive patients. HILL-BONE CHBPTS score correlated significantly in a positive direction with diastolic blood pressure, duration of treatment and the number of medications, As per JNC 8 recommendations, 58.9% (76) hypertensive patients were having blood pressure under control, whereas 41.1% (53) were having uncontrolled blood pressure. HILL-BONE CHBPTS scores were significantly higher (reflecting lower adherence) in hypertensive patients with uncontrolled blood pressure than those having optimally controlled blood pressure.Conclusions: Overall the medication adherence was poor in hypertensive patients. Adherence to therapeutic regimens is an important factor in blood pressure control among hypertensive patients and needs priority. Health education related to medication adherence needs be improved in hypertensive patients

Biodiesel from Thevetia peruviana seed oil with dimethyl carbonate using as an active catalyst potassium-methoxide

The transesterification of Thevetia peruviana seed oil with dimethyl carbonate (DMC) for preparing biodiesel has been studied using as an active catalyst potassium-methoxide (KOCH3). The effects of reaction conditions: Molar ratio of dimethyl carbonate to Thevetia peruviana seed oil, catalyst concentration, reaction time and agitation speed on dimethyl esters (DMC-Tp-BioDs) yield were investigated. The highest DMC-Tp-BioDs yield could reach 97.1% at refluxing temperature for 90 min with molar ratio of DMC-to-oil 5:1 and 2.0% w/w KOCH3 (based on oil weight). The fuel properties of the produced DMC-Tp-BioDs were compared with the ASTM D6751-02 biodiesel standard

An Ant Colony Optimization based Routing Techniques for VANET

With number of moving vehicles, vehicular Ad Hoc Network (VANET) is formed. These are provided with the wireless connections. Among various challenges in the VANET such as security and privacy of the messages, data forwarding is also considered as a major challenge. The effective communication is mainly depends on the how safely and fast the data is being forwarded among the vehicles. Data forwarding using Greedy mechanism suitable for routing in the VANETs, it depends only on the position of nodes and also data forwarding is done with minimum number of hops. In this paper, Position based GPCR and topology based DYMO routing protocol are adapted to make the use of Ant Colony Optimization (ACO) procedures. The resulting bio-inspired protocols, ACO_GPCR and ACO_DYMO had its performance evaluated and compared against existing GPCR and DYMO routing protocols. The obtained results suggest that making the use of ACO algorithm make these protocols more efficient in terms of Delay, Jitter, Packet Delivery Ratio and energy consumption

Observational cross-sectional study to evaluate the effects of self-medication with topical agents used by patients for superficial fungal skin infection at tertiary care hospital in Mumbai

Background: Superficial fungal infections of the hair, skin, and nails are a major cause of morbidity in the world and their incidence continues to increase. While self-medication is also one of the major health concerns for health authorities worldwide. The aim of the study was to evaluate the effects of topical agents used by patients for self-medication, to enlisting the topical agents used by patients for self-medication, to ensure by whose suggestion’s patients engaged in self-medication.Methods: It was an observational cross-sectional study which was conducted at the outpatient department (OPD) of dermatology in tertiary care hospital. A detailed history of the participant was recorded regarding topical agents used for self-medication and their effects were documented in a predesigned case record form.Results: The population consisted of 128 males and 87 females. All these 215 participants had used 61 topical agents of different brand names and the majority of were irritant, corticosteroids and antifungal much high in use. Among 215 participants, 111 had temporary relief from the infection and reoccurred after stopping its use. 98 showed aggravation in their infection among which 90 showed effects like irritation, burning and itching at the application site, while 8 participants showed dryness or flaking of skin.Conclusions: Self-medication is widely practiced among patients with a superficial fungal skin infection in Mumbai. A pharmacist was the major source for obtaining these topical agents. We can conclude, there is a need to counsel and discourage patients from self-medication and regulations for dispensing the prescribed drugs should be made stringent

Morphometric studies in the genus Clerodendrum L.

Six Clerodendrum L. species from Kolhapur district were morphometrically analyzed with the help of PCA, cluster analysis and CD. It was observed that the quantitative characters viz. petiole length, leaf length and leaf width have great significance in delimitation of all the species and corolla tube length, leaf width, gynoecium length and leaf length have great contribution in separation of the taxa. Clerodendrum multiflorum (Burm.f.) O. Ktze.- Clerodendrum inerme (L.) Gaertn., Clerodendrum paniculatum L. - Clerodendrum viscosum Vent. and Clerodendrum inerme (L.) Gaertn. - Clerodendrum serratum (L.) Moon. are very closely related with each other and Clerodendrum multiflorum (Burm.f.) O.Ktze.- Clerodendrum paniculatum L. and Clerodendrum multiflorum (Burm.f.) O. Ktze. - Clerodendrum viscosum Vent. are significantly different from each other

Anaesthetic management of neonate with giant occipital meningoencephalocele: case report

Meningoencephalocele is herniation of cerebrospinal fluid, brain tissue and meninges through the skull defect. The anaesthetic management of occipital meningoencephalocele is challenging because of the difficulty in securing airway, prone position, blood loss and, perioperative care. The two major aims of the anaesthesiologists while caring for children with occipital encephalocoele intraoperatively are to avoid premature rupture of the encephalocoele and to manage a possible difficult airway due to restricted neck movement and inability to achieve optimal position for intubation of the trachea. We report a case of giant occipital meningoencephalocele presented for surgical excision. Perioperative management of patients with giant meningoencephalocele may be challenging for both anaesthesiologist and neurosurgeon. These patients must be managed closely with an interdisciplinary approach