Chemical Composition of the Essential Oil and Antimicrobial Activity of Scaligeria DC. Taxa and Implications for Taxonomy

Six different Scaligeria DC. taxa (Apiaceae) essential oils (EOs) obtained by hydrodistillation from herba with the flowers collected from different sites from Turkey. The oils were analyzed and characterized by gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS) simultaneously. A total of 133 different compounds were identified and relative qualitative and quantitative differences were observed among the evaluated samples. Analytical profiles of the Scaligeria EOs showed characteristic differences in terms of different main chemical constituents, between the two taxa S. lazica Boiss. and S. tripartita (Kalen.) Tamamsch; and S. napiformis (Sprengel) Grande, S. meifolia (Fenzl) Boiss., S. capillifolia Post, S. hermonis Post, S. glaucescens (DC.) Boiss. taxa, respectively. The main component germacrene D can be utilized as marker for the chemical discrimination of the Scaligeria genus. In addition, Scaligeria EOs were evaluated in vitro for their antimicrobial activity against pathogenic Gram positive (Staphylococcus aureus and Bacillus cereus), Gram negative (Pseudomonas aeruginosa) and yeast (Candida albicans, C. parapsilosis, and C. krusei) standard strains by using a micro-dilution assay. As a general result, the oils showed moderate inhibitory range when compared with standard antimicrobial agents

CYTOTOXIC AND GENOTOXIC EVALUATION OF SOME NEWLY SYNTHESIZED SULFONAMIDE DERIVATIVES

In the present study, it was aimed to assess the genotoxic potentials of [4,4-Dimethyl-2,6dioxocyclohexylidene) methylamino) benzene sulfonamide] (2b) and [4-((1,3-Dimethy1-2,4,6trioxo-tetrahydropyrimidin-5(6H)-ylidene) methyla mino) benzenesulfonamide] (2e) compounds which were synthesized considering that they may be used as drug raw materials and detected to inhibit human carbonic anhydrase I, II isoenzymes. For this purpose, chromosomal aberration, micronucleus and comet tests were implemented in human peripheral blood lymphocytes. 2b was used at the concentrations of 2.12, 1.06, 0.53 mu g/mL. 2e was used at the concentrations of 2.52, 1.26, 0.63 mu g/mL for these in vitro assays. We observed that 2b and 2e had no significant difference in all our application doses for chromosomal abnormalities and micronucleus assay. 2b and 2e showed different responses for tail length, tail intensity and tail moment in Comet assay. 2b reduced the mitotic index in all concentrations in 48 h application compared to both control groups, whereas 2e only reduced mitotic index at 2.52 mu g/mL compared to negative control and in all concentrations compared to the solvent control. According to the obtained results, the test substances are cytotoxic at high concentrations and long-term exposure but they are not genotoxic in human peripheral lymphocytes