2,104 research outputs found
Rapid, quantitative determination of bacteria in water
A bioluminescent assay for ATP in water borne bacteria is made by adding nitric acid to a water sample with concentrated bacteria to rupture the bacterial cells. The sample is diluted with sterile, deionized water, then mixed with a luciferase-luciferin mixture and the resulting light output of the bioluminescent reaction is measured and correlated with bacteria present. A standard and a blank also are presented so that the light output can be correlated to bacteria in the sample and system noise can be substracted from the readings. A chemiluminescent assay for iron porphyrins in water borne bacteria is made by adding luminol reagent to a water sample with concentrated bacteria and measuring the resulting light output of the chemiluminescent reaction
Detection of Close-In Extrasolar Giant Planets Using the Fourier-Kelvin Stellar Interferometer
We evaluate the direct detection of extrasolar giant planets with a
two-aperture nulling infrared interferometer, working at angles
, and using a new `ratio-of-two-wavelengths' technique.
Simple arguments suggest that interferometric detection and characterization
should be quite possible for planets much closer than the conventional inner
working angle, or angular resolution limit. We show that the peak signal from a
nulling infrared interferometer of baseline ( meters) will often
occur `inside the null', and that the signal variations from path-difference
fluctuations will cancel to first order in the ratio of two wavelengths. Using
a new interferometer simulation code, we evaluate the detectability of all the
known extrasolar planets as observed using this two-color method with the
proposed {\it Fourier Kelvin Stellar Interferometer (FKSI)}. In its minimum
configuration {\it FKSI} uses two 0.5-meter apertures on a 12.5-meter baseline,
and a field-of-regard. We predict that known
extrasolar planets are directly detectable using {\it FKSI}, with
low-resolution spectroscopy () being possible in the most favorable
cases. Spaceborne direct detection of extrasolar giant planets is possible with
meter baselines, and does not require the much longer baselines
provided by formation flying.Comment: Accepted for publication in ApJ Letter
Determination of antimicrobial susceptibilities on infected urines without isolation
A method is described for the quick determination of the susceptibilities of various unidentified bacteria contained in an aqueous physiological fluid sample, particularly urine, to one or more antibiotics. A bacterial adenosine triphosphate (ATP) assay is carried out after the elimination of non-bacterial ATP to determine whether an infection exists. If an infection does exist, a portion of the sample is further processed, including subjecting parts of the portion to one or more antibiotics. Growth of the bacteria in the parts are determined, again by an ATP assay, to determine whether the unidentified bacteria in the sample are susceptible to the antibiotic or antibiotics under test
The Importance of Phase in Nulling Interferometry and a Three Telescope Closure-Phase Nulling Interferometer Concept
We discuss the theory of the Bracewell nulling interferometer and explicitly
demonstrate that the phase of the "white light" null fringe is the same as the
phase of the bright output from an ordinary stellar interferometer. As a
consequence a "closure phase" exists for a nulling interferometer with three or
more telescopes. We calculate the phase offset as a function of baseline length
for an Earth-like planet around the Sun at 10 pc, with a contrast ratio of
at 10 m. The magnitude of the phase due to the planet is radians, assuming the star is at the phase center of the array.
Although this is small, this phase may be observable in a three-telescope
nulling interferometer that measures the closure phase. We propose a simple
non-redundant three-telescope nulling interferometer that can perform this
measurement. This configuration is expected to have improved characteristics
compared to other nulling interferometer concepts, such as a relaxation of
pathlength tolerances, through the use of the "ratio of wavelengths" technique,
a closure phase, and better discrimination between exodiacal dust and planets
Application of firefly luciferase assay for adenosine triphosphate (ATP) to antimicrobial drug sensitivity testing
The development of a rapid method for determining microbial susceptibilities to antibiotics using the firefly luciferase assay for adenosine triphosphate (ATP) is documented. The reduction of bacterial ATP by an antimicrobial agent was determined to be a valid measure of drug effect in most cases. The effect of 12 antibiotics on 8 different bacterial species gave a 94 percent correlation with the standard Kirby-Buer-Agar disc diffusion method. A 93 percent correlation was obtained when the ATP assay method was applied directly to 50 urine specimens from patients with urinary tract infections. Urine samples were centrifuged first to that bacterial pellets could be suspended in broth. No primary isolation or subculturing was required. Mixed cultures in which one species was predominant gave accurate results for the most abundant organism. Since the method is based on an increase in bacterial ATP with time, the presence of leukocytes did not interfere with the interpretation of results. Both the incubation procedure and the ATP assays are compatible with automation
Quantifying structure in networks
We investigate exponential families of random graph distributions as a
framework for systematic quantification of structure in networks. In this paper
we restrict ourselves to undirected unlabeled graphs. For these graphs, the
counts of subgraphs with no more than k links are a sufficient statistics for
the exponential families of graphs with interactions between at most k links.
In this framework we investigate the dependencies between several observables
commonly used to quantify structure in networks, such as the degree
distribution, cluster and assortativity coefficients.Comment: 17 pages, 3 figure
Geometric View of Measurement Errors
The slope of the best fit line from minimizing the sum of the squared oblique
errors is the root of a polynomial of degree four. This geometric view of
measurement errors is used to give insight into the performance of various
slope estimators for the measurement error model including an adjusted fourth
moment estimator introduced by Gillard and Iles (2005) to remove the jump
discontinuity in the estimator of Copas (1972). The polynomial of degree four
is associated with a minimun deviation estimator. A simulation study compares
these estimators showing improvement in bias and mean squared error
Exoplanet Atmospheres
At the dawn of the first discovery of exoplanets orbiting sun-like stars in
the mid-1990s, few believed that observations of exoplanet atmospheres would
ever be possible. After the 2002 Hubble Space Telescope detection of a
transiting exoplanet atmosphere, many skeptics discounted it as a one-object,
one-method success. Nevertheless, the field is now firmly established, with
over two dozen exoplanet atmospheres observed today. Hot Jupiters are the type
of exoplanet currently most amenable to study. Highlights include: detection of
molecular spectral features; observation of day-night temperature gradients;
and constraints on vertical atmospheric structure. Atmospheres of giant planets
far from their host stars are also being studied with direct imaging. The
ultimate exoplanet goal is to answer the enigmatic and ancient question, "Are
we alone?" via detection of atmospheric biosignatures. Two exciting prospects
are the immediate focus on transiting super Earths orbiting in the habitable
zone of M-dwarfs, and ultimately the spaceborne direct imaging of true Earth
analogs.Comment: Annual Reviews of Astronomy and Astrophysics, in press. 47 pages, 16
Figures. This article was completed in January 2010; more recent references
are not include
The interaction of lean and building information modeling in construction
Lean construction and Building Information Modeling are quite different initiatives, but both are having profound impacts on the construction industry. A rigorous analysis of the myriad specific interactions between them indicates that a synergy exists which, if properly understood in theoretical terms, can be exploited to improve construction processes beyond the degree to which it might be improved by application of either of these paradigms independently. Using a matrix that juxtaposes BIM functionalities with prescriptive lean construction principles, fifty-six interactions have been identified, all but four of which represent constructive interaction. Although evidence for the majority of these has been found, the matrix is not considered complete, but rather a framework for research to
explore the degree of validity of the interactions. Construction executives, managers, designers and developers of IT systems for construction can also benefit from the framework as an aid to recognizing the potential synergies when planning their lean and BIM adoption strategies
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