Biostimulatory Action of Arbuscular Mycorrhizal Fungi Enhances Productivity, Functional and Sensory Quality in ‘Piennolo del Vesuvio’ Cherry Tomato Landraces

Arbuscular mycorrhizal fungi (AMF) are a promising tool to improve plant nutrient use efficiency (NUE) and tolerance against abiotic stresses. Moreover, AMF can potentially increase plant productivity and reduce the negative externalities of the agricultural sector. Our study aimed to elucidate whether AMF (containing Rhizoglomus irregulare and Funneliformis mosseae) could positively affect not only tomato growth and productivity but also the nutritional and nutraceutical quality of yellow-pigmented type (‘Giagiù’) and red-pigmented type (‘Lucariello’) tomatoes (Solanum lycopersicum L.). These cherry tomatoes are landraces of the Protected Designation of Origin (PDO) ‘Pomodorino del Piennolo del Vesuvio’ (PPV), one of the most typical agricultural products of the Campania region (Southern Italy). AMF rose fruit yield by increasing the number of fruits per plant (+49% and +29% in ‘Giagiù’ and ‘Lucariello’, respectively) but not of the fruit mean mass. AMF increased lycopene (+40%), total ascorbic acid (TAA; +41%), alanine (+162%), gamma-Aminobutyric acid (GABA; +101%) and branched-chain amino acids (BCAAs; +53%) in ‘Lucariello’. In ‘Giagiù’, AMF increased calcium (+63%), zinc (+45%), ASP (+70%), GABA (+53%) and the essential amino acids arginine (+58%) and lysine (+45%), also indicating a genotype-specific response. In both landraces, AMF improved nutrient uptake and biosynthesis of important molecules involved in the control the oxidative stress and cellular pH. In addition to the beneficial effects of human health, the molecules influenced by the AMF treatment are expected to extend the shelf life of tomato fruits, thus further promoting the useful agronomic application of AMF for premium tomatoes marketed fresh or in pendulums (‘piennoli’)

Salinity Duration Differently Modulates Physiological Parameters and Metabolites Profile in Roots of Two Contrasting Barley Genotypes

Hordeum maritimum With. is a wild salt tolerant cereal present in the saline depressions of the Eastern Tunisia, where it significantly contributes to the annual biomass production. In a previous study on shoot tissues it was shown that this species withstands with high salinity at the seedling stage restricting the sodium entry into shoot and modulating over time the leaf synthesis of organic osmolytes for osmotic adjustment. However, the tolerance strategy mechanisms of this plant at root level have not yet been investigated. The current research aimed at elucidating the morphological, physiological and biochemical changes occurring at root level in H. maritimum and in the salt sensitive cultivar Hordeum vulgare L. cv. Lamsi during five-weeks extended salinity (200 mM NaCl), salt removal after two weeks of salinity and non-salt control. H. maritimum since the first phases of salinity was able to compartmentalize higher amounts of sodium in the roots compared to the other cultivar, avoiding transferring it to shoot and impairing photosynthetic metabolism. This allowed the roots of wild plants to receive recent photosynthates from leaves, gaining from them energy and carbon skeletons to compartmentalize toxic ions in the vacuoles, synthesize and accumulate organic osmolytes, control ion and water homeostasis and re-establish the ability of root to grow. H. vulgare was also able to accumulate compatible osmolytes but only in the first weeks of salinity, while soon after the roots stopped up taking potassium and growing. In the last week of salinity stress, the wild species further increased the root to shoot ratio to enhance the root retention of toxic ions and consequently delaying the damages both to shoot and root. This delay of few weeks in showing the symptoms of stress may be pivotal for enabling the survival of the wild species when soil salinity is transient and not permanent

Spatial and Temporal Profile of Glycine Betaine Accumulation in Plants Under Abiotic Stresses

Several halophytes and a few crop plants, including Poaceae, synthesize and accumulate glycine betaine (GB) in response to environmental constraints. GB plays an important role in osmoregulation, in fact, it is one of the main nitrogen-containing compatible osmolytes found in Poaceae. It can interplay with molecules and structures, preserving the activity of macromolecules, maintaining the integrity of membranes against stresses and scavenging ROS. Exogenous GB applications have been proven to induce the expression of genes involved in oxidative stress responses, with a restriction of ROS accumulation and lipid peroxidation in cultured tobacco cells under drought and salinity, and even stabilizing photosynthetic structures under stress. In the plant kingdom, GB is synthesized from choline by a two-step oxidation reaction. The first oxidation is catalyzed by choline monooxygenase (CMO) and the second oxidation is catalyzed by NAD+-dependent betaine aldehyde dehydrogenase. Moreover, in plants, the cytosolic enzyme, named N-methyltransferase, catalyzes the conversion of phosphoethanolamine to phosphocholine. However, changes in CMO expression genes under abiotic stresses have been observed. GB accumulation is ontogenetically controlled since it happens in young tissues during prolonged stress, while its degradation is generally not significant in plants. This ability of plants to accumulate high levels of GB in young tissues under abiotic stress, is independent of nitrogen (N) availability and supports the view that plant N allocation is dictated primarily to supply and protect the growing tissues, even under N limitation. Indeed, the contribution of GB to osmotic adjustment and ionic and oxidative stress defense in young tissues, is much higher than that in older ones. In this review, the biosynthesis and accumulation of GB in plants, under several abiotic stresses, were analyzed focusing on all possible roles this metabolite can play, particularly in young tissues

Biostimulation as a Means for Optimizing Fruit Phytochemical Content and Functional Quality of Tomato Landraces of the San Marzano Area

The effect of plant biostimulation on fruits of traditional tomato germplasm is largely unknown. We examined how a tropical plant-derived biostimulant impacts the nutritional, functional, and compositional characteristics of tomato fruits from four landraces, collected in the San Marzano (SM) tomato Protected Designation of Origin (PDO) region, by profiling primary and secondary metabolites. Biostimulation was not able to completely reshuffle the morpho-physiological and nutritional profile of the four landraces. Their distinct phytochemical profile indicated a genotype specific tuning of the analyzed traits, which also included an improved yield and fruit quality. Biostimulation of SM1 and SM3 increased photosynthetic accumulation of carbohydrate reserves, improved mineral nutrient use efficiency and consequently, yield (+21% and 34%, respectively). Moreover, biostimulation augmented the nutraceutical properties of the SM2 landrace. Interestingly, the plant-derived product increased in all genotypes lycopene, but not polyphenol accumulation in fruits. Our results show the potential of biostimulatory applications towards optimizing the fruit quality of the acclaimed SM landraces, which is suitable to satisfy both the rising consumer demand for premium traditional tomatoes and the technological needs of the food industry

Ascophyllum nodosum Based Extracts Counteract Salinity Stress in Tomato by Remodeling Leaf Nitrogen Metabolism

Biostimulants have rapidly and widely been adopted as growth enhancers and stress protectants in agriculture, however, due to the complex nature of these products, their mechanism of action is not clearly understood. By using two algal based commercial biostimulants in combination with the Solanum lycopersicum cv. MicroTom model system, we assessed how the modulation of nitrogen metabolites and potassium levels could contribute to mediate physiological mechanisms that are known to occur in response to salt/and or osmotic stress. Here we provide evidence that the reshaping of amino acid metabolism can work as a functional effector, coordinating ion homeostasis, osmotic adjustment and scavenging of reactive oxygen species under increased osmotic stress in MicroTom plant cells. The Superfifty biostimulant is responsible for a minor amino acid rich-phenotype and could represent an interesting instrument to untangle nitrogen metabolism dynamics in response to salinity and/or osmotic stress

Effect of Thermal Stress on Tissue Ultrastructure and Metabolite Profiles During Initiation of Radiata Pine Somatic Embryogenesis

Climate change will inevitably lead to environmental variations, thus plant drought tolerance will be a determinant factor in the success of plantations and natural forestry recovery. Some metabolites, such as soluble carbohydrates and amino acids, have been described as being the key to both embryogenesis efficiency and abiotic stress response, contributing to phenotypic plasticity and the adaptive capacity of plants. For this reason, our main objectives were to evaluate if the temperature during embryonal mass initiation in radiata pine was critical to the success of somatic embryogenesis, to alter the morphological and ultrastructural organization of embryonal masses at cellular level and to modify the carbohydrate, protein, or amino acid contents. The first SE initiation experiments were carried out at moderate and high temperatures for periods of different durations prior to transfer to the control temperature of 23°C. Cultures initiated at moderate temperatures (30°C, 4 weeks and 40°C, 4 days) showed significantly lower initiation and proliferation rates than those at the control temperature or pulse treatment at high temperatures (50°C, 5 min). No significant differences were observed either for the percentage of embryogenic cell lines that produced somatic embryos, or for the number of somatic embryos per gram of embryonal mass. Based on the results from the first experiments, initiation was carried out at 40°C 4 h; 50°C, 30 min; and a pulse treatment of 60°C, 5 min. No significant differences were found for the initiation or number of established lines or for the maturation of somatic embryos. However, large morphological differences were observed in the mature somatic embryos. At the same time, changes observed at cellular level suggested that strong heat shock treatments may trigger the programmed cell death of embryogenic cells, leading to an early loss of embryogenic potential, and the formation of supernumerary suspensor cells. Finally, among all the differences observed in the metabolic profile, it is worth highlighting the accumulation of tyrosine and isoleucine, both amino acids involved in the synthesis of abiotic stress response-related secondary metabolites

Lactic acid production from tomato pomace fermentable sugars using innovative biological treatments

The organic residues from agro-food sector can be transformed into valuable economic resources rather than representing an unmanageable waste, whose disposal in landfills contributes to increase both soil pollution and the greenhouse effect. With this in mind, the aim of the investigation is to design eco-friendly compatible innovative biological processes for producing lactic acid (LA) from fermentable sugars using as substrate tomato pomace. In the first part of the investigation, we show the physico-chemical characterization of tomato pomace produced in the 2017 season with or without thermal pre-treatment. pH, total and volatile solids content, sugars and cellulose are measured. The microorganism consortia suitable for lactic acid production are selected from buffalo dung, and compared with commercial strains of lactic acid producing bacteria. Both bacteria consortia are used as inoculum for the anaerobic fermentation of tomato pomace as sole substrate for producing fermentable sugars and convert them in LA. Sugars are measured by a spectrophotometric-coupled enzyme assay and LA by HPLC with electrical conductivity detector. The molecular analysis of the isolated bacteria is performed by using the denaturing gradient gel electrophoresis (DGGE). Anaerobic fermentation of tomato pomace is also performed using different initial pH values of the substrate. The final goal is to design biological processes aimed at achieving the highest LA recovery yields

Metabolic Profile and Performance Responses of Ranunculus asiaticus L. Hybrids as Affected by Light Quality of Photoperiodic Lighting

R. asiaticus is a quantitative long day plant grown for cut flowers and flowering potted plants production. We evaluated the influence of light spectrum of three light sources for end-of-day photoperiodic treatments, with different phytochrome photoequilibria (PPE) induced at plant level, on metabolic profiling of two hybrids of Ranunculus asiaticus L., MBO and MDR, in plants from vernalized tuberous roots. The following treatments were compared to natural day length (NL): white fluorescent light (FL, PPE 0.84), LEDs Red:Far Red light at 3:1 ratio (R:FR 3:1, PPE, 0.84), and LEDs Red:Far Red light at 1:3 ratio (R:FR 1:3, PPE 0.63). Measurements were carried out to evaluate the time course of carbohydrate, amino acid and protein levels throughout the growing cycle in tuberous roots and leaves, in relation to the different plant stages (pre-planting, vegetative phase and flowering). The study of metabolic profiling suggested that the differences between the tuberous root reserves of the two R. asiaticus hybrids could be responsible for the capacity of MBO to exert an early flowering. In particular, the proton-consuming synthesis during pre-planting of two amino acids, alanine and aminobutyric acid (GABA), is able to buffer the cytoplasmic acidosis and pH altered by the vernalization process, and GABA istself can efficiently scavenge reactive oxygen species. This fast response to the stress caused by vernalization allows MBO plants to accelerate the process of vegetative development and flowering. Some other changes in metabolites profile were certainly related to the different response to day length and photoperiodic light quality in the two hybrids, like dose exerted by low R:FR lighting in both MBO and MDR. However, the most of responses are under a strict genetic control

Ascophyllum nodosum-based algal extracts act as enhancers of growth, fruit quality, and adaptation to stress in salinized tomato plants

Seaweeds extracts (SWE) are widely used to improve plant growth, quality and stress tolerance. However, the functional link between the complex composition of these products and their mechanisms of action has been only marginally addressed. A greenhouse experiment was performed on Microtom tomato plants in order to evaluate the effect of two Ascophyllum nodosum based algal derivatives, Rygex (R) and Super Fifty (SF), on a tomato exposed to salinity (0, 42.5 and 85 mM NaCl) and normal and reduced nutrient availability (100% and 70% of the standard regimen). Bioactive compounds with beneficial effects on growth and stress adaptation characterized via gas chromatography–mass spectrometry analysis (GC-MS). Enhanced growth of 13% was observed in Super Fifty treatment under a full-strength nutritional regimen, independent of the salinity treatment. Although Rygex and Super Fifty treatments did not significantly enhance plant growth and yield under salt treatment, they enhanced the accumulation of minerals, antioxidants, and essential amino acids in tomato fruits, with an overall improvement in nutritional value. Overall, SWE may affect and ameliorate different aspects of nutrition and stress tolerance and thus contribute to the sustainability of agricultural systems. Elucidating the link between bioactive compounds in SWE and plant responses will be critical to characterizing the mechanism of action of SWE.Seaweed extracts (SWE) are widely used to improve plant growth, fruit quality, and stress tolerance. However, the functional link between the complex composition of algal-based products and their mechanisms of action has been only marginally addressed. A greenhouse experiment was performed on Microtom tomato plants in order to evaluate the effect of two Ascophyllum nodosum-based algal derivatives, Rygex (R) and Super Fifty (SF), on a tomato exposed to salinity (0, 42.5, and 85 mM NaCl) and normal and reduced nutrient availability (100 and 70% of the standard regimen). Bioactive compounds, with possible beneficial effects on growth and stress adaptation, were characterized via gas chromatography-mass spectrometry analysis (GC-MS). Enhanced growth of 13% was observed with Super Fifty treatment under a full-strength nutritional regimen, independent of the salinity treatment. Although Rygex and Super Fifty treatments did not significantly enhance plant growth and yield under salt treatment, they enhanced the accumulation of minerals, antioxidants, and essential amino acids in tomato fruits, with an overall improvement in nutritional value. Overall, SWE may affect and ameliorate different aspects of nutrition and stress tolerance and thus contribute to the sustainability of agricultural systems. Elucidating the link between bioactive compounds in SWE and plant responses will be critical to characterizing the mechanism of action of SWE

Light spectral composition affects metabolic response and flowering in non-vernalized Ranunculus asiaticus L

We investigated the influence of photoperiodic light spectrum, inducing different phytochrome photoequilibria (PPE) at plant level, on photosynthesis, metabolic profiling, plant growth and flowering of Ranunculus asiaticus L. hybrids, MBO and MDR with different flowering earliness, grown in glasshouse from rehydrated dry tuberous roots. Plants were exposed to three photoperiodic treatments (day extension to 14 h), compared to natural day length (NL): white fluorescent light (PPE 0.84), and LEDs Red:Far Red light at 3:1 ratio (PPE 0.84) and 1:3 ratio (PPE 0.63). We discuss the results also compared to data on plants from rehydrated and vernalized roots previously reported in Modarelli et al., 2000a. Leaf gas exchanges and quantum yield of PSII electron transport were higher in MDR than in MBO, whereas non-photochemical quenching showed the opposite behaviour. In MDR, R:FR 3:1 light was the most effective in promoting stomatal conductance, while it reduced photochemistry and increased heat dissipation compared to other treatments. Under NL, leaf area was greater in MBO while flowering earliness and flower stems were similar in the hybrids. Photoperiodic treatments did not influence the plant growth while anticipated flowering in both the hybrids. In both the hybrids, lighting did not change the content of chlorophylls, carotenoids, glucose and sucrose. The greater number and expansion of leaves in MBO, except under R:FR 3:1, was accompanied by a lower photosynthetic capacity per leaf area. Conversely, in MDR, with lower leaf number, area and DW, an interesting increase of N-containing metabolites (i.e. chlorophylls and amino acids) occurred, thus exerting a positive effect on photosynthetic rate