The role of apoptosis in the pathogenesis of dermatitis herpetiformis.

Apoptosis is a form of cell death that is claimed to be involved in a number of chronic inflammatory and malignant skin diseases. The aim of this study was to investigate whether apoptosis may contribute to the pathogenesis of epidermal changes in dermatitis herpetiformis (DH) and, in particular, whether certain apoptosis-related markers such as Bax, Bcl-2, Fas and Fas ligand (FasL) take part in this process. For the detection of apoptotic nuclei, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labelling technique (TUNEL) was employed on cryostat sections. Skin lesions from six and perilesional skin from four DH patients were stained with monoclonal antibodies to Bax, Bcl-2, Fas and FasL. The same evaluation was also performed on three patients affected by bullous pemphigoid (BP) and in two healthy donors. Using TUNEL technique, a remarkable increase in the apoptotic rate within the epidermal compartment was observed in DH and BP patients in comparison with normal controls. In our immunohistochemical analysis, Bax/Bcl-2 ratio was almost the same in the epidermis of perilesional/lesional DH, BP and healthy skin specimens. In DH and BP specimens both Bax and Bcl-2 proteins were increased in the dermal perivascular compartment. Fas showed a prevalently epidermal staining, both in DH and BP lesions, while FasL was distributed in perivascular and subjunctional dermis; some FasL+ cells infiltrated the DEJ and the basal layer of epidermis. This study allowed us to highlight conspicuous apoptotic phenomena in basal and suprabasal keratinocytes within lesional and perilesional skin of DH. We conclude that in DH, as well as in BP, apoptosis plays a role in the pathogenesis of cutaneous lesions in concert with other pathogenetic mechanisms

Competence Centre ICDI per Open Science, FAIR, ed EOSC - Mission, Strategia e piano d'azione

This document presents the mission and strategy of the Italian Competence Centre on Open Science, FAIR, and EOSC. The Competence Centre is an initiative born within the Italian Computing and Data Infrastructure (ICDI), a forum created by representatives of major Italian Research Infrastructures and e-Infrastructures, with the aim of promoting sinergies at the national level, and optimising the Italian participation to European and global challenges in this field, including the European Open Science Cloud (EOSC), the European Data Infrastructure (EDI) and HPC. This working paper depicts the mission and objectives of the ICDI Competence Centre, a network of experts with various skills and competences that are supporting the national stakeholders on topics related to Open Science, FAIR principles application and participation to the EOSC. The different actors and roles are described in the document as well as the activities and services offered, and the added value each stakeholder can find the in Competence Centre. The tools and services provided, in particular the concept for the portal, though which the Centre will connect to the national landscape and users, are also presented

Mappatura CIDOC CRM per l’integrazione delle risorse del progetto RESTORE

The RESTORE project (smaRt accESs TO digital heRitage and mEmory) started in June 2020 with a duration of 2 years. The project consortium, coordinated by the Istituto Opera del Vocabolario Italiano - CNR (National Research Council of Italy), includes the State Archives and the Museum of Palazzo Pretorio in Prato, the Archival and Bibliographic Superintendency of Tuscany as well as the SPACE SpA company. The project - co-financed by the Region of Tuscany - has as its main purpose the recovery, integration and accessibility of data and digital objects produced in the last twenty years by its partners, in order to build a knowledge base with information on the history of the city and of its institutions, on the development of its economic and entrepreneurial system, and on the role of women in the development of a welfare state and network. Starting from the figure of the merchant Francesco di Marco Datini, his family and his entourage, and broadening the focus from the local dimension it will be possible to reconstruct a significant part of the history of European and Mediterranean cities of the 14th century, including commercial and economical aspects. This paper presents a focused overview on the mapping of the archival and museum digital resources provided by the partners (encoded with different standards, such as: EAD, EAC-CPF, TEI, ICCD etc.) to the CIDOC Conceptual Reference Model, the ontology chosen within the RESTORE project as the “common language” for semantic data integration. The landscape for the scientific and infrastructural development of the project’s activities consists in the collaboration (via the CNR- OVI) with some key players in the EU RIs environment, such as DARIAH-ERIC (ESFRI Landmark for the Humanities and Social Sciences) and E-RIHS (ESFRI project for the Heritage Science), as well as other actors within the EOSC Framework.Il progetto RESTORE (smaRt accESs TO digital heRitage and mEmory) è iniziato nel giugno 2020 con una durata di 2 anni. RESTORE è coordinato dall’Istituto Opera del Vocabolario Italiano del CNR, in partenariato con l’Archivio di Stato, il Museo di Palazzo Pretorio di Prato e la Soprintendenza Archivistica e Bibliografica della Toscana, con la partecipazione dell’azienda SPACE SpA nel ruolo di provider tecnologico. Il progetto - cofinanziato dalla Regione Toscana - ha come scopo principale il recupero, l’integrazione e l’accessibilità di dati e oggetti digitali che sono stati prodotti negli ultimi vent’anni anni dai suoi partner, al fine di costituire una base di conoscenza riguardante la storia della città e delle sue istituzioni, lo sviluppo del suo tessuto economico e imprenditoriale e, non da ultimo, il ruolo centrale giocato dalle donne nella gestione e creazione di scambi d’affari, di network e di contatti che favorissero l’ambiente cittadino generando occasioni di ulteriore ampliamento, sviluppo e benessere non solo economico, ma anche socio-culturale per la città di Prato. A partire dalla figura del mercante Francesco di Marco Datini, della sua famiglia e del suo entourage e muovendo da una dimensione solo apparentemente locale, sarà possibile ricostruire una parte rilevante della storia delle città d’Europa e del Mediterraneo del XIV secolo. Questo contributo si propone di offrire una panoramica della mappatura realizzata per le risorse archivistiche e museali fornite dai partner (modellate sugli standard EAD, EAC-CPF, ICCD, TEI) verso il modello concettuale CIDOC, ontologia scelta da RESTORE come “linguaggio comune” per l’integrazione semantica dei dati. Il contesto di riferimento per le attività scientifiche e di sviluppo infrastrutturale è costituito dalla collaborazione (attraverso il CNR-OVI) con alcune delle più rilevanti infrastrutture di ricerca a livello europeo, come DARIAH-ERIC (ESFRI Landmark per le discipline umanistiche e le scienze sociali) ed E-RIHS (progetto ESFRI per la scienza del patrimonio), oltre che dalla Cloud Europea della Ricerca (EOSC)

D8.3 Trustworthy Digital Repository status update and certification solutions for SSHOC repositories

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Parthenos D3.2 Report on Guidelines for Common Policies Implementation (Final)

The aim of this report is to present to its stakeholders (researchers, policy makers, cultural heritage institutions, research infrastructures, archives) a series of recommendations and guidelines about which policies to apply during and after their research or infrastructure work. “During their research work”, because policies on data and repositories guide the data creator to produce high quality data; “after their research work”, because policies on access and reuse help make the data more accessible and reusable. The research communities are: Archaeology, History, Language related studies and social sciences

SSHOC D8.2 Certification plan for SSHOC repositories

This report is the first deliverable of Task 8.2 “Trust & Quality Assurance” within WP8 of the SSHOC project. The distributed character of data infrastructures within the SSHOC communities requires developing an agreed approach to assessing the trustworthiness and quality of data repositories. This deliverable provides an overview of Trusted Digital Repository (TDR) standards offering a certification framework for communities represented in the SSHOC project (CESSDA, CLARIN, DARIAH, E-RIHS). Moreover, the deliverable lays the ground for the SSHOC trust work that is needed in order to facilitate the adoption of TDR standards and the FAIR principles in SSH data repositories across the board. In this report, ‘trust’ refers to the landscape of issues, standards and processes related to trustworthy digital repositories. Trust between all parties in the quality of data and services is critical for research infrastructure in terms of people, processes and technologies. The level of trustworthiness can be assessed through evaluation against agreed requirements