Maternal-child co-participation in physical activity-related behaviours: prevalence and cross-sectional associations with mothers and children\u27s objectively assessed physical activity levels

BACKGROUND: Co-participation in physical activity may be a useful strategy for increasing physical activity in mothers and their young children, yet little empirical evidence exists on this topic for young families. This study aimed to identify the prevalence of mother-child co-participation in physical activity and examine the association between co-participatory behaviours and objectively-assessed physical activity in young children and their mothers. METHODS: One-hundred twenty-three 4-6 year-old children and their mothers were recruited from preschools in Belgium between November 2010 and January 2011. Mothers completed a questionnaire assessing the frequency of co-participation in five activities. Both mothers and children wore ActiGraph GT1M accelerometers concurrently for 7 days to assess the time spent in moderate-to vigorous-intensity physical activity (MVPA) and light- to vigorous-intensity physical activity (LMVPA). Descriptive statistics (means, frequencies) were used to determine the prevalence of co-participation. Separate multiple linear regression analyses examined the association between co-participation and mothers\u27 and children\u27s physical activity on weekdays and weekends. RESULTS: Most mothers reported infrequent co-participation in physical activities with their children. On weekdays, walking or cycling for short trips was positively associated with children\u27s MVPA while attending a park or similar more than once per week was negatively associated with children\u27s MVPA and LMVPA. Going to an indoor play centre together once or more per week was negatively associated with mother\u27s LMVPA. On weekends, walking or cycling with their child in their free time was positively associated with both children\u27s and mothers\u27 MVPA and childrens\u27 LMVPA. Going to an indoor play centre together 1-3 times/month was negatively associated with children\u27s weekend MVPA. CONCLUSIONS: Reported rates of co-participation in mothers and their preschool children were low. The association with maternal and child physical activity may be dependent on the co-participatory behaviour assessed and may differ between weekday and weekends. Promoting walking and cycling together during leisure time may be an effective strategy to increase both preschool children\u27s and mothers\u27 MVPA

Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies

Expansion microscopy (ExM) enables imaging of preserved specimens with nanoscale precision on diffraction-limited instead of specialized super-resolution microscopes. ExM works by physically separating fluorescent probes after anchoring them to a swellable gel. The first ExM method did not result in the retention of native proteins in the gel and relied on custom-made reagents that are not widely available. Here we describe protein retention ExM (proExM), a variant of ExM in which proteins are anchored to the swellable gel, allowing the use of conventional fluorescently labeled antibodies and streptavidin, and fluorescent proteins. We validated and demonstrated the utility of proExM for multicolor super-resolution (~70 nm) imaging of cells and mammalian tissues on conventional microscopes.United States. National Institutes of Health (1R01GM104948)United States. National Institutes of Health (1DP1NS087724)United States. National Institutes of Health ( NIH 1R01EY023173)United States. National Institutes of Health (1U01MH106011

Advances in Mobile Health for Inflammatory Bowel Disease.

Mobile health has the potential to transform the management of chronic illnesses, expanding treatment from a purely clinic-based approach to a more patient-centered delivery of care. For patients with inflammatory bowel disease (IBD), a condition characterized by a relapsing and remitting course, adoption of mobile health strategies can promote improved quality of care delivery and clinical outcomes. Benefits of mobile health applications for IBD include tracking symptoms to guide disease management, coordinating data exchange across clinical care providers, increasing communication between patients and the care team, and providing educational materials to increase patient engagement and satisfaction. In this review, we present the current offerings for telemedicine systems and mobile applications designed for patients with IBD and discuss the potential advantages and limitations of utilizing mobile health in the care of these patients

Advances in Mobile Health for Inflammatory Bowel Disease

Mobile health has the potential to transform the management of chronic illnesses, expanding treatment from a purely clinic-based approach to a more patient-centered delivery of care. For patients with inflammatory bowel disease (IBD), a condition characterized by a relapsing and remitting course, adoption of mobile health strategies can promote improved quality of care delivery and clinical outcomes. Benefits of mobile health applications for IBD include tracking symptoms to guide disease management, coordinating data exchange across clinical care providers, increasing communication between patients and the care team, and providing educational materials to increase patient engagement and satisfaction. In this review, we present the current offerings for telemedicine systems and mobile applications designed for patients with IBD and discuss the potential advantages and limitations of utilizing mobile health in the care of these patients

Advances in Mobile Health for Inflammatory Bowel Disease

Mobile health has the potential to transform the management of chronic illnesses, expanding treatment from a purely clinic-based approach to a more patient-centered delivery of care. For patients with inflammatory bowel disease (IBD), a condition characterized by a relapsing and remitting course, adoption of mobile health strategies can promote improved quality of care delivery and clinical outcomes. Benefits of mobile health applications for IBD include tracking symptoms to guide disease management, coordinating data exchange across clinical care providers, increasing communication between patients and the care team, and providing educational materials to increase patient engagement and satisfaction. In this review, we present the current offerings for telemedicine systems and mobile applications designed for patients with IBD and discuss the potential advantages and limitations of utilizing mobile health in the care of these patients

Sexual desire in female-to-male transsexual persons: exploration of the role of testosterone

Objective: To describe sexual desire in female-to-male transsexual persons post sex reassignment surgery (SRS). The associations between serum androgen levels and sexual desire are examined. Design: Single center cross-sectional study. Methods: Forty-five female-to-male transsexual persons post SRS completed a standardized questionnaire assessing sexual desire (Sexual Desire Inventory). In addition, participants were asked questions on sexual desire before starting hormone treatment and having SRS. Serum levels of testosterone, LH and sex hormone-binding globulin were measured on fasting morning serum samples. Results: In retrospect, 73.9% of the participants reported an increase in sexual desire after hormone treatment and SRS. Solitary sexual desire scores were significantly correlated with frequency of masturbation (r=0.835; P<0.001), whereas frequency of sexual intercourse with a partner was not. No direct associations were found between testosterone and solitary or dyadic sexual desire. However, ANOVA showed an independent effect of LH on solitary sexual desire (P<0.001). Post hoc analysis revealed that female-to-male transsexual persons with elevated levels of LH, indicating suboptimal testosterone therapy, reported significantly lower solitary sexual desire levels (than those with low LH levels; PZ0.007). Suppressed LH levels were also associated with having a higher need for sexual activities (P=0.009) and a higher frequency of excessive sexual desire (P=0.007). Conclusion: Most female-to-male transsexual persons report on a marked increase in sexual desire after testosterone treatment and SRS. No direct associations between levels of testosterone and solitary or dyadic sexual desire were found. However, measures of sexual desire were inversely associated with LH levels