636 research outputs found
Neuropeptide AF and FF modulation of adipocyte metabolism. Primary insights from functional genomics and effects on beta-adrenergic responsiveness.
The presence of a neuropeptide AF and FF receptor (NPFF-R2) mRNA in human adipose tissue (Elshourbagy, N. A., Ames, R. S., Fitzgerald, L. R., Foley, J. J., Chambers, J. K., Szekeres, P. G., Evans, N. A., Schmidt, D. B., Buckley, P. T., Dytko, G. M., Murdock, P. R., Tan, K. B., Shabon, U., Nuthulaganti, P., Wang, D. Y., Wilson, S., Bergsma, D. J., and Sarau, H. M. (2000) J. Biol. Chem. 275, 25965-25971) suggested these peptides, principally recognized for their pain modulating effects, may also impact on adipocyte metabolism, an aspect that has not been explored previously. Our aim was thus to obtain more insights into the actions of these peptides on adipocytes, an approach initially undertaken with a functional genomic assay. First we showed that 3T3-L1 adipocytes express both NPFF-R1 and NPFF-R2 transcripts, and that NPAF binds adipocyte membranes with a nanomolar affinity as assessed by surface plasmon resonance technology. Then, and following a 24-h treatment with NPFF or NPAF (1 microm), we have measured using real-time quantitative reverse transcriptase-PCR the mRNA steady state levels of already well characterized genes involved in key pathways of adipose metabolism. Among the 45 genes tested, few were modulated by NPFF ( approximately 10%) and a larger number by NPAF ( approximately 27%). Interestingly, NPAF increased the mRNA levels of beta2- and beta3-adrenergic receptors (AR), and to a lesser extent those of beta1-ARs. These variations in catecholamine receptor mRNAs correlated with a clear induction in the density of beta2- and beta3-AR proteins, and in the potency of beta-AR subtype-selective agonists to stimulate adenylyl cyclase activity. Altogether, these data show that NPFF-R1 and NPFF-R2 are functionally present in adipocytes and suggest that besides their well described pain modulation effects, NPAF and to a lesser extent NPFF, may have a global impact on body energy storage and utilization
Invariant Regularization of Anomaly-Free Chiral Theories
We present a generalization of the Frolov-Slavnov invariant regularization
scheme for chiral fermion theories in curved spacetimes. local gauge symmetries
of the theory, including local Lorentz invariance. The perturbative scheme
works for arbitrary representations which satisfy the chiral gauge anomaly and
the mixed Lorentz-gauge anomaly cancellation conditions. Anomalous theories on
the other hand manifest themselves by having divergent fermion loops which
remain unregularized by the scheme. Since the invariant scheme is promoted to
also include local Lorentz invariance, spectator fields which do not couple to
gravity cannot be, and are not, introduced. Furthermore, the scheme is truly
chiral (Weyl) in that all fields, including the regulators, are left-handed;
and only the left-handed spin connection is needed. The scheme is, therefore,
well suited for the study of the interaction of matter with all four known
forces in a completely chiral fashion. In contrast with the vectorlike
formulation, the degeneracy between the Adler-Bell-Jackiw current and the
fermion number current in the bare action is preserved by the chiral
regularization scheme.Comment: 28pgs, LaTeX. Typos corrected. Further remarks on singlet current
Degenerate Rotating Black Holes, Chiral CFTs and Fermi Surfaces I - Analytic Results for Quasinormal Modes
In this work we discuss charged rotating black holes in
that degenerate to extremal black holes with zero entropy. These black holes
have scaling properties between charge and angular momentum similar to those of
Fermi surface operators in a subsector of SYM. We add a
massless uncharged scalar to the five dimensional supergravity theory, such
that it still forms a consistent truncation of the type IIB ten dimensional
supergravity and analyze its quasinormal modes. Separating the equation of
motion to a radial and angular part, we proceed to solve the radial equation
using the asymptotic matching expansion method applied to a Heun equation with
two nearby singularities. We use the continued fraction method for the angular
Heun equation and obtain numerical results for the quasinormal modes. In the
case of the supersymmetric black hole we present some analytic results for the
decay rates of the scalar perturbations. The spectrum of quasinormal modes
obtained is similar to that of a chiral 1+1 CFT, which is consistent with the
conjectured field-theoretic dual. In addition, some of the modes can be found
analytically.Comment: 41 pages, 1 figure, LaTeX; v2: typos corrected, references adde
Thermal and electrical conductivity of iron at Earth's core conditions
The Earth acts as a gigantic heat engine driven by decay of radiogenic
isotopes and slow cooling, which gives rise to plate tectonics, volcanoes, and
mountain building. Another key product is the geomagnetic field, generated in
the liquid iron core by a dynamo running on heat released by cooling and
freezing to grow the solid inner core, and on chemical convection due to light
elements expelled from the liquid on freezing. The power supplied to the
geodynamo, measured by the heat-flux across the core-mantle boundary (CMB),
places constraints on Earth's evolution. Estimates of CMB heat-flux depend on
properties of iron mixtures under the extreme pressure and temperature
conditions in the core, most critically on the thermal and electrical
conductivities. These quantities remain poorly known because of inherent
difficulties in experimentation and theory. Here we use density functional
theory to compute these conductivities in liquid iron mixtures at core
conditions from first principles- the first directly computed values that do
not rely on estimates based on extrapolations. The mixtures of Fe, O, S, and Si
are taken from earlier work and fit the seismologically-determined core density
and inner-core boundary density jump. We find both conductivities to be 2-3
times higher than estimates in current use. The changes are so large that core
thermal histories and power requirements must be reassessed. New estimates of
adiabatic heat-flux give 15-16 TW at the CMB, higher than present estimates of
CMB heat-flux based on mantle convection; the top of the core must be thermally
stratified and any convection in the upper core driven by chemical convection
against the adverse thermal buoyancy or lateral variations in CMB heat flow.
Power for the geodynamo is greatly restricted and future models of mantle
evolution must incorporate a high CMB heat-flux and explain recent formation of
the inner core.Comment: 11 pages including supplementary information, two figures. Scheduled
to appear in Nature, April 201
Determination of the (3x3)-Sn/Ge(111) structure by photoelectron diffraction
At a coverage of about 1/3 monolayer, Sn deposited on Ge(111) below 550 forms
a metastable (sqrt3 x sqrt3)R30 phase. This phase continuously and reversibly
transforms into a (3x3) one, upon cooling below 200 K. The photoemission
spectra of the Sn 4d electrons from the (3x3)-Sn/Ge(111) surface present two
components which are attributed to inequivalent Sn atoms in T4 bonding sites.
This structure has been explored by photoelectron diffraction experiments
performed at the ALOISA beamline of the Elettra storage ring in Trieste
(Italy). The modulation of the intensities of the two Sn components, caused by
the backscattering of the underneath Ge atoms, has been measured as a function
of the emission angle at fixed kinetic energies and viceversa. The bond angle
between Sn and its nearest neighbour atoms in the first Ge layer (Sn-Ge1) has
been measured by taking polar scans along the main symmetry directions and it
was found almost equivalent for the two components. The corresponding bond
lengths are also quite similar, as obtained by studying the dependence on the
photoelectron kinetic energy, while keeping the photon polarization and the
collection direction parallel to the Sn-Ge1 bond orientation (bond emission). A
clear difference between the two bonding sites is observed when studying the
energy dependence at normal emission, where the sensitivity to the Sn height
above the Ge atom in the second layer is enhanced. This vertical distance is
found to be 0.3 Angstroms larger for one Sn atom out of the three contained in
the lattice unit cell. The (3x3)-Sn/Ge(111) is thus characterized by a
structure where the Sn atom and its three nearest neighbour Ge atoms form a
rather rigid unit that presents a strong vertical distortion with respect to
the underneath atom of the second Ge layer.Comment: 10 pages with 9 figures, added reference
Three-body structure of low-lying 18Ne states
We investigate to what extent 18Ne can be descibed as a three-body system
made of an inert 16O-core and two protons. We compare to experimental data and
occasionally to shell model results. We obtain three-body wave functions with
the hyperspherical adiabatic expansion method. We study the spectrum of 18Ne,
the structure of the different states and the predominant transition strengths.
Two 0+, two 2+, and one 4+ bound states are found where they are all known
experimentally. Also one 3+ close to threshold is found and several negative
parity states, 1-, 3-, 0-, 2-, most of them bound with respect to the 16O
excited 3- state. The structures are extracted as partial wave components, as
spatial sizes of matter and charge, and as probability distributions.
Electromagnetic decay rates are calculated for these states. The dominating
decay mode for the bound states is E2 and occasionally also M1.Comment: 17 pages, 5 figures (version to appear in EPJA
Differences in bleeding behavior after endoscopic band ligation: a retrospective analysis
<p>Abstract</p> <p>Background</p> <p>Endoscopic band ligation (EBL) is generally accepted as the treatment of choice for bleeding from esophageal varices. It is also used for secondary prophylaxis of esophageal variceal hemorrhage. However, there is no data or guidelines concerning endoscopic control of ligation ulcers. We conducted a retrospective study of EBL procedures analyzing bleeding complications after EBL.</p> <p>Methods</p> <p>We retrospectively analyzed data from patients who underwent EBL. We analyzed several data points, including indication for the procedure, bleeding events and the time interval between EBL and bleeding.</p> <p>Results</p> <p>255 patients and 387 ligation sessions were included in the analysis. We observed an overall bleeding rate after EBL of 7.8%. Bleeding events after elective treatment (3.9%) were significantly lower than those after treatment for acute variceal hemorrhage (12.1%). The number of bleeding events from ligation ulcers and variceal rebleeding was 14 and 15, respectively. The bleeding rate from the ligation site in the group who underwent emergency ligation was 7.1% and 0.5% in the group who underwent elective ligation. Incidence of variceal rebleeding did not vary significantly. Seventy-five percent of all bleeding episodes after elective treatment occurred within four days after EBL. 20/22 of bleeding events after emergency ligation occured within 11 days after treatment. Elective EBL has a lower risk of bleeding from treatment-induced ulceration than emergency ligation.</p> <p>Conclusions</p> <p>Patients who underwent EBL for treatment of acute variceal bleeding should be kept under medical surveillance for 11 days. After elective EBL, it may be reasonable to restrict the period of surveillance to four days or even perform the procedure in an out-patient setting.</p
Artificial immune systems
The biological immune system is a robust, complex, adaptive system that defends the body from foreign pathogens. It is able to categorize all cells (or molecules) within the body as self or nonself substances. It does this with the help of a distributed task force that has the intelligence to take action from a local and also a global perspective using its network of chemical messengers for communication. There are two major branches of the immune system. The innate immune system is an unchanging mechanism that detects and destroys certain invading organisms, whilst the adaptive immune system responds to previously unknown foreign cells and builds a response to them that can remain in the body over a long period of time. This remarkable information processing biological system has caught the attention of computer science in recent years
The Standard Model with gravity couplings
In this paper, we examine the coupling of matter fields to gravity within the
framework of the Standard Model of particle physics. The coupling is described
in terms of Weyl fermions of a definite chirality, and employs only
(anti)self-dual or left-handed spin connection fields. It is known from the
work of Ashtekar and others that such fields can furnish a complete description
of gravity without matter. We show that conditions ensuring the cancellation of
perturbative chiral gauge anomalies are not disturbed. We also explore a global
anomaly associated with the theory, and argue that its removal requires that
the number of fundamental fermions in the theory must be multiples of 16. In
addition, we investigate the behavior of the theory under discrete
transformations P, C and T; and discuss possible violations of these discrete
symmetries, including CPT, in the presence of instantons and the
Adler-Bell-Jackiw anomaly.Comment: Extended, and replaced with LaTex file. 25 Page
CMB Telescopes and Optical Systems
The cosmic microwave background radiation (CMB) is now firmly established as
a fundamental and essential probe of the geometry, constituents, and birth of
the Universe. The CMB is a potent observable because it can be measured with
precision and accuracy. Just as importantly, theoretical models of the Universe
can predict the characteristics of the CMB to high accuracy, and those
predictions can be directly compared to observations. There are multiple
aspects associated with making a precise measurement. In this review, we focus
on optical components for the instrumentation used to measure the CMB
polarization and temperature anisotropy. We begin with an overview of general
considerations for CMB observations and discuss common concepts used in the
community. We next consider a variety of alternatives available for a designer
of a CMB telescope. Our discussion is guided by the ground and balloon-based
instruments that have been implemented over the years. In the same vein, we
compare the arc-minute resolution Atacama Cosmology Telescope (ACT) and the
South Pole Telescope (SPT). CMB interferometers are presented briefly. We
conclude with a comparison of the four CMB satellites, Relikt, COBE, WMAP, and
Planck, to demonstrate a remarkable evolution in design, sensitivity,
resolution, and complexity over the past thirty years.Comment: To appear in: Planets, Stars and Stellar Systems (PSSS), Volume 1:
Telescopes and Instrumentatio
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