Generation of a human iPS cell line from a patient with retinitis pigmentosa due to EYS mutation

Retinitis pigmentosa (RP) is an inherited retinal degenerative disease. Mutations in EYS have been associated with autosomal recessive RP. The human iPS cell line, CABi002-A, derived from peripheral blood mononuclear cells from a patient carrying a heterozygous double mutation in EYS gene was generated by non-integrative reprogramming technology, using hOCT3/4, hSOX2, hc-MYC and hKLF4 reprogramming factors. Pluripotency and differentiation capacity were assessed by immunocytochemistry and RT-PCR. This iPSC line can be further differentiated towards the affected cells to understand the pathophysiology of the disease and test new therapeutic strategies.Cellex FoundationFundación Progreso y Salu

Gestión integrada de la calidad, medio ambiente, seguridad y salud laboral bajo la triple E y el paradigma holónico fractal

El presente trabajo tiene como objetivo plantear una propuesta que contribuya a la creación de un Sistema Integrado de Gestión de calidad, medioambiente, seguridad y salud laboral apoyándose en el paradigma holónico y construyendo una propuesta incardinada en la Triple E. Con la implantación de las normas ISO 9001, ISO 14001 y OHSAS 18001 (ISO 45001) desde un enfoque sistémico, las empresas son consideradas como un conjunto de procesos que tienen que ser gestionados con todas sus variables, de manera eficaz y eficiente, para así visibilizarse en el mercado como actuante responsable a lo que demandan todas las partes interesadas. Esta situación promueve dar respuesta a la necesidad que poseen en la actualidad las empresas de encontrar herramientas que faciliten la gestión de los procesos de manera responsable e integral. Se propone un marco de trabajo donde desarrollar una propuesta a través de una herramienta informática que haga realidad esta integración.The objective of this work is to develop a proposal that contributes to the creation of an Integrated Management System for quality, environment, safety and occupational health, based on the holonic paradigm and building a proposal included in the Triple E. With the implementation of the ISO 9001, ISO 14001 and OHSAS 18001 (ISO 45001) standards from a systemic approach, companies are considered as a set of processes that have to be managed with all their variables, in an effective and efficient manner, in order to become visible in the market as a responsible actor to what all interested parties demand. This situation promotes a response to the need that companies currently must find tools that facilitate the management of processes in a responsible and comprehensive manner. It proposes a framework where to develop a proposal through a computer tool that makes this integration a reality

High-Throughput System for the Early Quantification of Major Architectural Traits in Olive Breeding Trials Using UAV Images and OBIA Techniques

The need for the olive farm modernization have encouraged the research of more efficient crop management strategies through cross-breeding programs to release new olive cultivars more suitable for mechanization and use in intensive orchards, with high quality production and resistance to biotic and abiotic stresses. The advancement of breeding programs are hampered by the lack of efficient phenotyping methods to quickly and accurately acquire crop traits such as morphological attributes (tree vigor and vegetative growth habits), which are key to identify desirable genotypes as early as possible. In this context, an UAV-based high-throughput system for olive breeding program applications was developed to extract tree traits in large-scale phenotyping studies under field conditions. The system consisted of UAV-flight configurations, in terms of flight altitude and image overlaps, and a novel, automatic, and accurate object-based image analysis (OBIA) algorithm based on point clouds, which was evaluated in two experimental trials in the framework of a table olive breeding program, with the aim to determine the earliest date for suitable quantifying of tree architectural traits. Two training systems (intensive and hedgerow) were evaluated at two very early stages of tree growth: 15 and 27 months after planting. Digital Terrain Models (DTMs) were automatically and accurately generated by the algorithm as well as every olive tree identified, independently of the training system and tree age. The architectural traits, specially tree height and crown area, were estimated with high accuracy in the second flight campaign, i.e. 27 months after planting. Differences in the quality of 3D crown reconstruction were found for the growth patterns derived from each training system. These key phenotyping traits could be used in several olive breeding programs, as well as to address some agronomical goals. In addition, this system is cost and time optimized, so that requested architectural traits could be provided in the same day as UAV flights. This high-throughput system may solve the actual bottleneck of plant phenotyping of "linking genotype and phenotype," considered a major challenge for crop research in the 21st century, and bring forward the crucial time of decision making for breeders

Nanobiosensors based on optoelectronic and nanomechanical transducers for genomic and proteomic applications

5 páginas, 10 figuras.-- PACS: 42.25.-p; 42.82.-m; 85.85.+[EN] We show the design, fabrication and testing of micro/nanobiosensor devices based on highly sensitive optoelectronic and nanomechanical transducers. Most of the devices are fabricated by standard Silicon CMOS microelectronics technology according to a precise design for achieving a high sensitivity for biosensing applications. Three biosensors have been developed: (a) a Surface Plasmon Resonance biosensor, (b) an integrated, Mach-Zehnder micro/nano interferometer device based on optical waveguides, and (c) nanomechanical biosensors based on microcantilevers.[ES] En este artículo se muestra el diseño, la fabricación y la caracterización de dispositivos micro/nanobiosensores basados en transductores optoelectrónicos y nanomecánicos. La mayoría de los dispositivos se fabrican con tecnología estándar de Si compatible CMOS, después de un cuidadoso diseño de los transductores para conseguir biosensores de alta sensibilidad. Se muestran tres tipos de biosensors: (a) un biosensor de Resonancia de Plasmón Superficial (b) un micro/nano Ínterferómetro integrado Mach-Zehnder basado en guías de ondas ópticas y (c) biosensores nanomecánicos basados en micropalancas.Peer reviewe

Diversity of plant growth-promoting bacteria associated with sugarcane

The sugarcane (Saccharum spp) presents economic importance, mainly for tropical regions, being an important Brazilian commodity. However, this crop is strongly dependent on fertilizers, mainly nitrogen (N). This study assessed the plant growth-promoting bacteria (PGPB) associated with sugarcane that could be used as a potential inoculant to the crop. We evaluated the genetic diversity of PGPB in the plant tissue of sugarcane varieties (RB 867515, RB 1011, and RB 92579). The primer BOX-A1R was used to differentiate the similar isolated and further sequencing 16S rRNA ribosomal gene. The 16S rRNA gene showed the presence of seven different genera distributed into four groups, the genus Bacillus, followed by Paenibacillus (20%), Burkholderia (14%), Herbaspirillum (6%), Pseudomonas (6%), Methylobacterium (6%), and Brevibacillus (3%). The molecular characterization of endophytic isolates from sugarcane revealed a diversity of bacteria colonizing this plant, with a possible biotechnological potential to be used as inoculant and biofertilizers

Sostenibilidad 4.0: análisis del ciclo de la vida integrado del producto inteligente conectado

La sostenibilidad se sitúa como un aspecto central en la industria con el desarrollo de la fabricación sostenible donde la tendencia de minimizar los impactos producidos por el desarrollo de productos con el objetivo de crear un ecosistema global sostenible supone el nuevo contexto para los productos de la Cuarta Revolución Industrial (Industria 4.0). La Industria 4.0 promueve la transformación de las organizaciones productivas del siglo XXI hacia nuevas concepciones de los sistemas de fabricación denominados inteligentes. El nuevo yacimiento de valor es la conectividad entre todos los agentes ciber-físicos (personas, procesos y sistemas) que crean unas nuevas redes de valor inteligentes conectadas en tiempo real, monitorizadas, controladas y organizadas dinámicamente. Este trabajo propone un nuevo marco de trabajo basado en el paradigma de la economía circular para evaluar la sostenibilidad en tiempo real a través de un análisis del ciclo de vida integrado en las tres dimensiones (economía, ecología y equidad) donde analizar y procesar los datos recogidos en tiempo real en el ciclo de vida del producto junto con aquellos datos provenientes de las bases de datos estandarizadas y el histórico de datos generados en el ciclo de vida del producto a través de una Cloud Manufacturing Platform.Sustainability is placed as a central aspect in the industry with the development of sustainable manufacturing where the tendency to minimize the impacts produced by the development of products with the aim of creating a sustainable global ecosystem is the new context for the products of the Fourth Industrial Revolution (Industry 4.0). Industry 4.0 promotes the transformation of the productive organizations of the 21st century towards new conceptions of manufacturing systems called intelligent. The new value deposit is the connectivity between all the cyber-physical agents (people, processes and systems) that create new smart value networks connected in real time, monitored, controlled and dynamically organized. This work proposes a new framework based on the circular economy paradigm to evaluate sustainability in real time through an analysis of the life cycle integrated in the three dimensions (economy, ecology and equity) in which to analyze and process the data collected in real time in the life cycle of the product together with data from the standardized databases and the historical data generated in the life cycle of the product through a Cloud Manufacturing Platform.Plan Propio de la Universidad de Sevilla Proyecto: 2017/0000096

Sistema inteligente inalámbrico para análisis y monitorización de líneas de tensión subterráneas en Smart-grids (SIIAM)

El mantenimiento de la infraestructura eléctrica supone un enorme coste para las compañías. Por ello, es una línea de investigación activa el desarrollo de sistemas de monitorización de dicha infraestructura. Lamentablemente, es inevitable que se sigan produciendo algunos desperfectos, debido a agentes externos y al propio desgaste de las instalaciones. Estos problemas provocan las “faltas” (que es la interrupción del suministro eléctrico en una zona afectada). Ante dichas faltas las compañías deben actuar con la mayor celeridad posible (identificando la posición de la falta y reconfigurando la red). No obstante, a pesar de los avances existentes en monitorización de líneas eléctricas, la localización de una “falta” no es un problema sencillo, requiriendo una gran inversión de recursos materiales y humanos. Una de las infraestructuras en las que más se agrava este problema es en las líneas de media tensión subterráneas, ya que su monitorización actualmente está poco desarrollada. Por ello, en el marco del proyecto SIIAM se han investigado diversas soluciones para la monitorización de este tipo de líneas de distribución, para las cuales actualmente se está finalizando el desarrollo de un prototipo preindustrial, que permita monitorizar y facilite la localización en caso de falta.The maintenance of electrical structure to guarantee the proper functioning of service, pose a huge cost for the electrical enterprises. This causes that monitoring of the electrical infrastructure becomes an active research line, so we can reach a fast error detection and reduce the electrical lines problems. Unfortunately, it is impossible to avoid damages in the system caused by external agents, that causes electrical installation wear away. These Problems produce “faults” (Interruption of energy supply over one affected area). The companies need to fix this fault quickly (The Company need to locate the fault and reconfigure the electrical network, trying to restore the energy supply to the bigger number of clients). However, the fault location need to employ a lot of workers and materials, despite nowadays the monitoring process is very advanced. This problem is worse in the medium voltage underground lines, because of fault monitoring devices are little developed here. In SIIAM Project, we have studied some solutions to fix monitoring problem of underground medium voltage lines. Currently, we have finished a prototype that can monitor and locate fault in medium voltage underground lines without the need to damage the cable or its shield.Plan Propio de la Universidad de Sevilla Proyecto: 2017/00000962Ministerio de Economía y Competitividad (España) TEC2013-40767-