Reversed-Phase HPLC Determination of Cholesterol in Food Items.

Cholesterol is a fat-like molecule found among lipids in animal (including human) tissues. It is needed for maintaining good health. However, health issues have been raised because of the strong correlation between high levels of cholesterol in the body and cardiovascular disease. An HPLC method for quantitative determination of cholesterol in foods is presented. This involves a C-18 stationary phase using a 70:30 methanol: 2-propanol mobile phase with an UV detector set at 212 nm. The method showed linearity in the range 5.0 to 100.0 μg/mL and also good reproducibility with relative standard deviation of 4.22%, 2.71%, 4.8%, and 3.7% for the different samples analyzed. The mean recovery of the butter sample was 106.5%. The samples under investigation were common food items such as butter, lard, and two different types of cheese

Nineteenth-Century Literature in Transition: The 1830s

This instalment in the Nineteenth-Century Literature in Transition series concerns a decade that was as technologically transitional as it was eventful on a global scale. It collects work from a group of internationally renowned scholars across disciplinary boundaries in order to engage with the wide array of cultural developments that defined the 1830s. Often overlooked as a boundary between the Romantic and Victorian periods, this decade was, the book proposes, the central pivot of the nineteenth century. Far from a time of peaceful reform, it was marked by violent colonial expansion, political resistance, and revolutionary technologies such as the photograph, the expansion of steam power, and the railway that changed the world irreversibly. Contributors explore a flurry of cultural forms to take the pulse of the decade, from Silver Fork fiction to lithography, from working-class periodicals to photographs, and from urban sketches to magazine fiction.</p