In silico biomechanical design of the metal frame of transcatheter aortic valves: multi-objective shape and cross-sectional size optimization

Transcatheter aortic valve (TAV) implantation has become an established alternative to open-hearth surgical valve replacement. Current research aims to improve the treatment safety and extend the range of eligible patients. In this regard, computational modeling is a valuable tool to address these challenges, supporting the design phase by evaluating and optimizing the mechanical performance of the implanted device. In this study, a computational framework is presented for the shape and cross-sectional size optimization of TAV frames. Finite element analyses of TAV implantation were performed in idealized aortic root models with and without calcifications, implementing a mesh-morphing procedure to parametrize the TAV frame. The pullout force magnitude, peak maximum principal stress within the aortic wall, and contact pressure in the left ventricular outflow tract were defined as objectives of the optimization problem to evaluate the device mechanical performance. Design of experiment coupled with surrogate modeling was used to define an approximate relationship between the objectives and the TAV frame parameters. Surrogate models were interrogated within a fixed design space and multi-objective design optimization was conducted. The investigation of the parameter combinations within the design space allowed the successful identification of optimized TAV frame geometries, suited to either a single or groups of aortic root anatomies. The optimization framework was efficient, resulting in TAV frame designs with improvedmechanical performance, ultimately leading to enhanced procedural outcomes and reduced costs associated with the device iterative development cycle

Click-connected 2-(hydroxyimino)aldehydes for the design of UV-responsive functional molecules

Click chemistry is used to functionalize simple lipophilic and water-soluble molecules, a complex PEGylated phospholipid (DSPE-PEG2000), and two benzylic substrates with the 2-(hydroxyimino)aldehyde (HIA) group. To this end, two terminal alkynes bearing the HIA moiety were synthesized and coupled to different azides through copper(I)-catalyzed azide alkyne cycloaddition (CuAAC). Norrish–Yang photoisomerization (λ= 365 nm, LED source) is successfully obtained, with no interference by the triazole linker, except when the forbidden n-π* carbonyl transition is screened by a remote substituent such as salicylaldehyde. UV-Vis spectrometry suggests a specific interaction of HIAs with Cu(II), whereas no such evidence is found with Cu(I). We thereby show that the CuAAC methodology can be used successfully to obtain HIA-based UV-responsive hydrophilic or lipophilic ligands, phospholipidic components for the construction of liposomes, and macrocycle precursors. © 2020 Wiley-VCH Gmb

Compact and tunable stretch bioreactor advancing tissue engineering implementation. Application to engineered cardiac constructs

Physical stimuli are crucial for the structural and functional maturation of tissues both in vivo and in vitro . In tissue engineering applications, bioreactors have become fundamental and effective tools for provid- ing biomimetic culture conditions that recapitulate the native physical stimuli. In addition, bioreactors play a key role in assuring strict control, automation, and standardization in the production process of cell-based products for future clinical application. In this study, a compact, easy-to-use, tunable stretch bioreactor is proposed. Based on customizable and low-cost technological solutions, the bioreactor was designed for providing tunable mechanical stretch for biomimetic dynamic culture of different engineered tissues. In-house validation tests demonstrated the accuracy and repeatability of the imposed mechanical stimulation. Proof of concepts biological tests performed on engineered cardiac constructs, based on de- cellularized human skin scaffolds seeded with human cardiac progenitor cells, confirmed the bioreactor Good Laboratory Practice compliance and ease of use, and the effectiveness of the delivered cyclic stretch stimulation on the cardiac construct maturation

A low-cost 3D-printed sample-holder for stirring-based decellularization of biological tissues

An innovative, low-cost, 3D-printed sample-holder is proposed for reproducible and effective stirring-based decellularization of biological tissues. The sample-holder was designed to be low-cost, easy to use with conventional laboratory equipment, and manufacturable through 3D printing. During stirring-based decellularization, the sample holder exposes the samples to convective flow, enhancing the reagent transport while protecting the samples from disruptive forces. Computational fluid dynamics analyses were carried out to elucidate the developing hydrodynamics. Explanatory tests, performed on human cardiac tissue samples, demonstrated the effectiveness of the presented device

A low-cost scalable 3D-printed sample-holder for agitation-based decellularization of biological tissues

Decellularized extracellular matrix is one of the most promising biological scaffold supporting in vitro tissue growth and in vivo tissue regeneration in both preclinical research and clinical practice. In case of thick tissues or even organs, conventional static decellularization methods based on chemical or enzymatic treatments are not effective in removing the native cellular material without affecting the extracellular matrix. To overcome this limitation, dynamic decellularization methods, mostly based on perfusion and agitation, have been proposed. In this study, we developed a low-cost scalable 3D-printed sample-holder for agitation-based decellularization purposes, designed for treating multiple specimens simultaneously and for improving efficiency, homogeneity and reproducibility of the decellularization treatment with respect to conventional agitation-based approaches. In detail, the proposed sample-holder is able to house up to four specimens and, immersed in the decellularizing solution within a beaker placed on a magnetic stirrer, to expose them to convective flow, enhancing the solution transport through the specimens while protecting them. Computational fluid dynamics analyses were performed to investigate the fluid phenomena establishing within the beaker and to support the sample-holder design. Exploratory biological tests performed on human skin specimens demonstrated that the sample-holder reduces process duration and increases treatment homogeneity and reproducibility

Design of Super-Resolving Toraldo Pupils for Radio Astronomical Applications

More than half a century ago, in 1952, Giuliano Toraldo di Francia suggested that the resolving power of an optical instrument could be improved using a filter consisting of finite-width concentric coronae of different amplitude and phase transmittance, now known as Toraldo Pupils (TPs). The concept of “super-resolution” was born, and in the cur- rent literature it is generally associated with various meth- ods for improving the angular resolution of an optical imag- ing system beyond the classical diffraction limit. In the mi- crowave range, the first successful laboratory test of TPs was performed in 2003. These first results suggested that TPs could represent a viable approach to achieve super- resolution in Radio Astronomy. We have therefore started a project devoted to an exhaustive study of TPs and how they could be implemented on a radio telescope. In this work we present a summary of the status of this project, and then we describe a preliminary design to implement a TP system on a 32-m radio telescope

FIRST TESTS OF A TORALDO PUPIL OPTICAL MODULE FOR THE 32M MEDICINA ANTENNA

Toraldo Pupils can improve the angular resolving power of an optical instrument beyond the classical diffraction limit (hence the term “super-resolution”) using a filter consisting of finite- width concentric coronae with different amplitude and phase transmittance. Toraldo Pupils represent a viable approach to achieve super-resolution on antennas and radio telescopes. In this work we present a summary of the electromagnetic simulations and laboratory tests of a prototype optical module based on a Toraldo Pupil that has been field-tested on the Medicina 32-m radio telescope

The Sardinia Radio Telescope Front-Ends

The 64 m diameter Sardinia Radio Telescope (SRT) has recently started an early science program using three cryogenic front-ends covering four bands: P-band (305-410 MHz), L-band (1.3-1.8 GHz), high C-band (5.7-7.7 GHz), K-band (18-26.5 GHz). The L- and the P-bands can be observed simultaneously with a single coaxial receiver installed at the primary focus, while a seven beam K-band receiver and a mono-feed high C-band receiver are installed, respectively at the secondary and beam waveguide focus. Additional front-ends are under construction to further expand the telescope observing capabilities. We report on the design and performance of the front-ends already installed on SRT and give an overview of the new ones to be completed in the near future

Status of the radio receiver system of the Sardina Radio Telescope

In this article, we present the design and performances of the radio receiver system installed at the Sardinia Radio Telescope (SRT). The three radio receivers planned for the first light of the Sardinian Telescope have been installed in three of the four possible focus positions. A dual linear polarization coaxial receiver that covers two frequency bands, the P-band (305-410 MHz) and the L-band (1.3-1.8 GHz) is installed at the primary focus. A mono-feed that covers the High C-band (5.7-7.7 GHz) is installed at the beam waveguide foci. A multi-beam (seven beams) K-band receiver (18- 26.5 GHz) is installed at the Gregorian focus. Finally, we give an overview about the radio receivers, which under test and under construction and which are needed for expanding the telescope observing capabilities

Front-Ends and Phased Array Feeds for the Sardinia Radio Telescope

We describe the design and performance of the Front- Ends for the 64-m diameter Sardinia Radio Telescope (SRT). An early science program was completed with SRT in August 2016, following a successful technical and scientific commissioning of the telescope and of its instrumentation. We give an overview of the three cryogenic Front-Ends, covering four bands, that were deployed on SRT during the early science program: P-band (305-410 MHz), L-band (1.3-1.8 GHz), high C-band (5.7- 7.7 GHz) and K-band (18-26.5 GHz). In addition, we describe the cryogenic Front-Ends that are currently under development, among which a seven beam for S-band (3.0-4.5 GHz) a mono-feed for Low-Cband (4.2-5.6 GHz), a 19-element for Q-band (33-50 GHz) and a mono-feed for a 3 mm band. Finally, we describe the development status of a demonstrator of a cryogenic C-band Phased Array Feed (PAF) for potential use at the SRT primary focus