Utilizing Data and Knowledge Mining for Probabilistic Knowledge Bases

Problems can arise whenever inferencing is attempted on a knowledge base that is incomplete. Our work shows that data mining techniques can be applied to fill in incomplete areas in Bayesian Knowledge Bases (BKBs), as well as in other knowledge-based systems utilizing probabilistic representations. The problem of inconsistency in BKBs has been addressed in previous work, where reinforcement learning techniques from neural networks were applied. However, the issue of automatically solving incompleteness in BKBs has yet to be addressed. Presently, incompleteness in BKBs is repaired through the application of traditional knowledge acquisition techniques. We show how association rules can be extracted from databases in order to replace excluded information and express missing relationships. A methodology for incorporating those results while maintaining a consistent knowledge base is also included

Trade with the People\u27s Republic of China: Current Status and Future Prospects

Since the normalization of diplomatic relations with the People\u27s Republic of China (PRC) in 1979, U.S. trade with China has developed rapidly. In particular, 1980 saw significant developments in the institutional framework of U.S.-China trade relations, with the signing of several new trade-related bilateral agreements and a number of high-level visits in both directions.2 At the same time, China has begun to update its domestic legal system as it plans to integrate more fully into the international economic order.3 The last year has also witnessed the cancellation of certain large development projects, and delay in the implementation of others, as the Chinese readjust their economic plans and priorities. This Perspective will first discuss the current status of U.S.-China trade relations, with an emphasis on the developments of 1980. It will then present our view of the Chinese economy and prospects for the future development of China\u27s foreign trade. Changes in the Chinese legal system will be mentioned only briefly, as those matters are treated in detail elsewhere in this issue

A hidden alkaline and carbonatite province of early carboniferous age in northeast Poland: Zircon U-Pb and pyrrhotite Re-Os geochronology

Extensive geophysical investigations in NE Poland in the 1950s and 1960s led to the discovery of an alkaline and carbonatite magmatic province buried under thick (600-800 m) Meso-Cenozoic cover north of the Trans-European Suture Zone, or Tornquist Line. Drilling focused on geophysical anomalies identified three intrusions in the Paleoproterozoic metasedimentary and metavolcanic rocks of the Mazowsze Domain: the Pisz gabbro-syenite massif, the Ełk syenite massif, and the small, differentiated Tajno body consisting of clinopyroxenite cumulates and syenites crosscut by carbonatite veins. Emplacement ages for these intrusions have been obtained by (1) zircon U-Pb geochronology on a gabbro from Pisz, a syenite from Ełk, and an albitite from Tajno and (2) a Re-Os model age for pyrrhotite from a Tajno carbonatite. The ages measured by both methods fall in the narrow range 354-345 Ma (Early Carboniferous: Tournaisian). This is slightly younger than the Late Devonian (380-360 Ma) Kola Peninsula alkaline and carbonatite province (20 intrusions) of NW Russia and Karelia but is of comparable age to the first manifestations of the long-lasting (~100 m.yr.) Carboniferous to Permian magmatic event (360-250 Ma) manifest in northern Europe (from the British Isles to southern Scandinavia, the North Sea, and northern Germany) in the foreland of the Variscan orogeny (in the so-called West European Carboniferous Basin) and the East European Craton

Transcriptional Auto-Regulation of RUNX1 P1 Promoter

RUNX1 a member of the family of runt related transcription factors (RUNX), is essential for hematopoiesis. The expression of RUNX1 gene is controlled by two promoters; the distal P1 promoter and the proximal P2 promoter. Several isoforms of RUNX1 mRNA are generated through the use of both promoters and alternative splicing. These isoforms not only differs in their temporal expression pattern but also exhibit differences in tissue specificity. The RUNX1 isoforms derived from P2 are expressed in a variety of tissues, but expression of P1-derived isoform is restricted to cells of hematopoietic lineage. However, the control of hematopoietic-cell specific expression is poorly understood. Here we report regulation of P1-derived RUNX1 mRNA by RUNX1 protein. In silico analysis of P1 promoter revealed presence of two evolutionary conserved RUNX motifs, 0.6kb upstream of the transcription start site, and three RUNX motifs within 170bp of the 5\u27UTR. Transcriptional contribution of these RUNX motifs was studied in myeloid and T-cells. RUNX1 genomic fragment containing all sites show very low basal activity in both cell types. Mutation or deletion of RUNX motifs in the UTR enhances basal activity of the RUNX1 promoter. Chromatin immunoprecipitation revealed that RUNX1 protein is recruited to these sites. Overexpression of RUNX1 in non-hematopoietic cells results in a dose dependent activation of the RUNX1 P1 promoter. We also demonstrate that RUNX1 protein regulates transcription of endogenous RUNX1 mRNA in T-cell. Finally we show that SCL transcription factor is recruited to regions containing RUNX motifs in the promoter and the UTR and regulates activity of the RUNX1 P1 promoter in vitro. Thus, multiple lines of evidence show that RUNX1 protein regulates its own gene transcription

\u3cem\u3eShewanella oneidensis\u3c/em\u3e Cytochrome c Nitrite Reductase (ccNiR) Does Not Disproportionate Hydroxylamine to Ammonia and Nitrite, Despite a Strongly Favorable Driving Force

Cytochrome c nitrite reductase (ccNiR) from Shewanella oneidensis, which catalyzes the six-electron reduction of nitrite to ammonia in vivo, was shown to oxidize hydroxylamine in the presence of large quantities of this substrate, yielding nitrite as the sole free nitrogenous product. UV–visible stopped-flow and rapid-freeze-quench electron paramagnetic resonance data, along with product analysis, showed that the equilibrium between hydroxylamine and nitrite is fairly rapidly established in the presence of high initial concentrations of hydroxylamine, despite said equilibrium lying far to the left. By contrast, reduction of hydroxylamine to ammonia did not occur, even though disproportionation of hydroxylamine to yield both nitrite and ammonia is strongly thermodynamically favored. This suggests a kinetic barrier to the ccNiR-catalyzed reduction of hydroxylamine to ammonia. A mechanism for hydroxylamine reduction is proposed in which the hydroxide group is first protonated and released as water, leaving what is formally an NH2+ moiety bound at the heme active site. This species could be a metastable intermediate or a transition state but in either case would exist only if it were stabilized by the donation of electrons from the ccNiR heme pool into the empty nitrogen p orbital. In this scenario, ccNiR does not catalyze disproportionation because the electron-donating hydroxylamine does not poise the enzyme at a sufficiently low potential to stabilize the putative dehydrated hydroxylamine; presumably, a stronger reductant is required for this

Correlations between the Electronic Properties of \u3cem\u3eShewanella oneidensis\u3c/em\u3e Cytochrome \u3cem\u3ec\u3c/em\u3e Nitrite Reductase (ccNiR) and Its Structure: Effects of Heme Oxidation State and Active Site Ligation

The electrochemical properties of Shewanella oneidensis cytochrome c nitrite reductase (ccNiR), a homodimer that contains five hemes per protomer, were investigated by UV–visible and electron paramagnetic resonance (EPR) spectropotentiometries. Global analysis of the UV–vis spectropotentiometric results yielded highly reproducible values for the heme midpoint potentials. These midpoint potential values were then assigned to specific hemes in each protomer (as defined in previous X-ray diffraction studies) by comparing the EPR and UV–vis spectropotentiometric results, taking advantage of the high sensitivity of EPR spectra to the structural microenvironment of paramagnetic centers. Addition of the strong-field ligand cyanide led to a 70 mV positive shift of the active site’s midpoint potential, as the cyanide bound to the initially five-coordinate high-spin heme and triggered a high-spin to low-spin transition. With cyanide present, three of the remaining hemes gave rise to distinctive and readily assignable EPR spectral changes upon reduction, while a fourth was EPR-silent. At high applied potentials, interpretation of the EPR spectra in the absence of cyanide was complicated by a magnetic interaction that appears to involve three of five hemes in each protomer. At lower applied potentials, the spectra recorded in the presence and absence of cyanide were similar, which aided global assignment of the signals. The midpoint potential of the EPR-silent heme could be assigned by default, but the assignment was also confirmed by UV–vis spectropotentiometric analysis of the H268M mutant of ccNiR, in which one of the EPR-silent heme’s histidine axial ligands was replaced with a methionine