The CPLEAR Electromagnetic Calorimeter

A large-acceptance lead/gas sampling electromagnetic calorimeter (ECAL) was constructed for the CPLEAR experiment to detect photons from decays of $\pi^0$s with momentum $p_{\pi^0} \le 800$ MeV$/c$. The main purpose of the ECAL is to determine the decay vertex of neutral-kaon decays \ko \rightarrow \pi^0\pi^0 \rightarrow 4 \gamma and \ko \rightarrow \pi^0\pi^0\pi^0 \rightarrow 6 \gamma. This requires a position-sensitive photon detector with high spatial granularity in $r$-, $\varphi$-, and $z$-coordinates. The ECAL --- a barrel without end-caps located inside a magnetic field of 0.44 T --- consists of 18 identical concentric layers. Each layer of $1/3$ radiation length (X${_0}$) contains a converter plate followed by small cross-section high-gain tubes of 2640 mm active length which are sandwiched by passive pick-up strip plates. The ECAL, with a total of $6$ X${_0}$, has an energy resolution of $\sigma (E)/E \approx 13\% / \sqrt{E(\mathrm{GeV})}$ and a position resolution of 4.5 mm for the shower foot. The shower topology allows separation of electrons from pions. The design, construction, read-out electronics, and performance of the detector are described

A method for detergent-free isolation of membrane proteins in their local lipid environment.

Despite the great importance of membrane proteins, structural and functional studies of these proteins present major challenges. A significant hurdle is the extraction of the functional protein from its natural lipid membrane. Traditionally achieved with detergents, purification procedures can be costly and time consuming. A critical flaw with detergent approaches is the removal of the protein from the native lipid environment required to maintain functionally stable protein. This protocol describes the preparation of styrene maleic acid (SMA) co-polymer to extract membrane proteins from prokaryotic and eukaryotic expression systems. Successful isolation of membrane proteins into SMA lipid particles (SMALPs) allows the proteins to remain with native lipid, surrounded by SMA. We detail procedures for obtaining 25 g of SMA (4 d); explain the preparation of protein-containing SMALPs using membranes isolated from Escherichia coli (2 d) and control protein-free SMALPS using E. coli polar lipid extract (1-2 h); investigate SMALP protein purity by SDS-PAGE analysis and estimate protein concentration (4 h); and detail biophysical methods such as circular dichroism (CD) spectroscopy and sedimentation velocity analytical ultracentrifugation (svAUC) to undertake initial structural studies to characterize SMALPs (∼2 d). Together, these methods provide a practical tool kit for those wanting to use SMALPs to study membrane proteins

The CPLEAR detector at CERN

The CPLEAR collaboration has constructed a detector at CERN for an extensive programme of CP-, T- and CPT-symmetry studies using ${\rm K}^0$ and $\bar{\rm K}^0$ produced by the annihilation of $\bar{\rm p}$'s in a hydrogen gas target. The ${\rm K}^0$ and $\bar{\rm K}^0$ are identified by their companion products of the annihilation ${\rm K}^{\pm} \pi^{\mp}$ which are tracked with multiwire proportional chambers, drift chambers and streamer tubes. Particle identification is carried out with a liquid Cherenkov detector for fast separation of pions and kaons and with scintillators which allow the measurement of time of flight and energy loss. Photons are measured with a lead/gas sampling electromagnetic calorimeter. The required antiproton annihilation modes are selected by fast online processors using the tracking chamber and particle identification information. All the detectors are mounted in a 0.44 T uniform field of an axial solenoid of diameter 2 m and length 3.6 m to form a magnetic spectrometer capable of full on-line reconstruction and selection of events. The design, operating parameters and performance of the sub-detectors are described.

DESIGN AND TEST OF A PROTOTYPE GAS-SAMPLING ELECTROMAGNETIC CALORIMETER OF HIGH GRANULARITY FOR THE CPLEAR EXPERIMENT

We have designed and tested a gas-sampling calorimeter of high granularity as a prototype for the electromagnetic calorimeter of the CPLEAR experiment. The prototype calorimeter, consisting of 18 layers of 1.5 mm thin lead converters interleaved with a total of 1152 high-gain tubes and 2304 pick-up strips, was tested in a tagged photon beam with photons in the energy range of 50 to 350 MeV. It well fulfilled the requirements of a good detection efficiency for photons above 50 MeV with a spatial resolution sigma almost-equal-to 5 mm for the photon conversion point and an energy resolution sigma(E)/E almost-equal-to 15% square-root E[GeV]

The CPLEAR detector at CERN

The CPLEAR collaboration has constructed a detector at CERN for an extensive programme of CP-, T- and CPT-symmetry studies using K-0 and <(K)over bar (0)> produced by the annihilation of <(p)over bar ‘s> in a hydrogen gas target. The K-0 and <(K)over bar (0)> are identified by their companion products of the annihilation K(+/-)pi(-/+) which are tracked with multiwire proportional chambers, drift chambers and streamer tubes. Particle identification is carried out with a liquid Cherenkov detector for fast separation of pions and kaons and with scintillators which allow the measurement of time of flight and energy loss. Photons are measured with a lead/gas sampling electromagnetic calorimeter. The required antiproton annihilation modes are selected by fast online processors using the tracking chamber and particle identification information. All the detectors are mounted in a 0.44 T uniform field of an axial solenoid of diameter 2 m and length 3.6 m to form a magnetic spectrometer capable of full on-line reconstruction and selection of events. The design, operating parameters and performance of the subdetectors are described

Study of the background in the measuring station at the n_TOF facility at CERN: sources and solutions

A background roughly two orders of magnitude higher than tolerable was found in the n_TOF facility at CERN during the first measurements [1]. This note describes a series of additional measurements performed in the n_TOF experimental area to study the origin and the characteristics of the background. The program of these measurements was determined taking into account the results from the simulations carried out by the EET group [2]. A first phase of measurements confirmed what was expected from the simulations, namely that the dominant source of background was due to neutrons generated by negative muon capture. Actions to reduce the background were taken according to the results from both measurements and simulations. An iron shielding wall 3.2 m thick was then placed in between the sweeping magnet and the second collimator, with the purpose of stopping most of the muons. In a second phase of measurements, results showed that the additional shielding reduced the main component of the background by about a factor of 30