Limitations of cross-talk between osteosarcoma and bone marrow-derived mesenchymal stem cells

Objectives: Metastasis is a multi-step process which leads the tumor cells to escape from primer tumor region due to their need to gain malign phenotypes. While the effect of bone marrow-derived mesenchymal stem cells upon metastasis is not certain, some studies point out bone marrow-derived mesenchymal stem cells (BM-MSCs) to have this ability due to cell-cell interaction, released cytokines, and organization with the extracellular matrix in the micro-environment. Cross-talk via soluble factors also shifts the metastatic character. Patients and Methods: In this study, the effects of mesenchymal stem cells on tumor behavior by creating different microenvironments in 3-dimensional (3D) in vitro cancer model is analyzed. The BM-MSCs and osteosarcoma cells were co-cultured via hanging-drop modeled 3D structure in normoxic and hypoxic conditions, and the cross-talk was modeled to measure their chemoattractant effects The invasion and migration rates were measured with xCELLigence DP real-time cell analysis system. Mann Whitney U Test was used to compare independent samples. All P-values <0.05 were considered statistically significant. Results: In this study, the most effective chemoattractant that increases the rate of migration in the osteosarcoma cell line under both normoxic (P 0.02) and hypoxic (P 0.004) conditions have been found to be the chemoattractant obtained from the BMSC culture. Conclusion: Soluble factors secreted by BM- MSCs to micro-environment are highly effective chemoattractants for osteosarcoma, nevertheless the stem cells that have been co-cultured with the MG-63 decrease this behavior. These results could provide a new scientific approach to downregulate the metastasis induced by the effect of BM-MSCs.Dokuz Eylul UniversityTailor of Science Biotechnology Innovation I.C

Salivary Pro-Inflammatory Cytokines and Salivary Bacterial Challenge Effect on Dental Caries: A Clinico-Molecular Cross-Sectional Study

Objectives: Objective of this study is to determine the correlation between salivary cytokine concentrations and cariogram value high (C1) and low (C2) experience in people. in this direction, saliva cytokines showed a significant correlation with the patient’s caries experience.Patients and Methods: Unstimulated whole saliva samples were collected from 38 appropriate populations of patients aged between 20 to 57 years old. All patients’ DMF (decay/missing/filled) values were higher than 0. Twenty-one caries activity groups (C1) and 17 caries non-activity groups (C2) patients were collected for this study. Procedures of the clinical evaluation were the collection of saliva samples and oral examination. the whole saliva from 38 patients with C1 and C2 was investigated for the existence of GM-CSF, TNF?, IFN-g, IL-2, IL-4, IL-6, IL-8, and IL-10.Results: the study population consisted of 38 subjects with a mean age of 34.76±14.43 years. Likewise, there was a significant interaction between salivary flow rate, S. Mutans number, number of Lactobacillus, number of meals and IL-6, a significant difference in caries activity groups.Conclusion: Salivary cytokines demonstrated significant interactions to caries experience of the patient