Amino acid sequence and distribution of mRNA encoding a major skeletal muscle laminin binding protein: an extracellular matrix-associated protein with an unusual COOH-terminal polyaspartate domain.

Two cDNAs encoding an abundant chicken muscle extracellular matrix (ECM)-associated laminin-binding protein (LBP) have been isolated and sequenced. The predicted primary amino acid sequence includes a probable signal peptide and a site for N-linked glycosylation, but lacks a hydrophobic segment long enough to span the membrane. The COOH terminus consists of an unusual repeat of 33 consecutive aspartate residues. Comparison with other sequences indicates that this protein is different from previously described LBPs and ECM receptors. RNA blot analysis of LBP gene expression showed that LBP mRNA was abundant in skeletal and heart muscle, but barely detectable in other tissues. Blots of chicken genomic DNA suggest that a single gene encodes this LBP. The amino acid sequence and mRNA distribution are consistent with the biochemical characterization described by Hall and co-workers (Hall, D. E., K. A. Frazer, B. C. Hahn, and L. F. Reichardt. 1988. J. Cell Biol. 107:687-697). These analyses indicate that LBP is an abundant ECM-associated muscle protein with an unusually high negative charge that interacts with both membranes and laminin, and has properties of a peripheral, not integral membrane protein. Taken together, our studies show that muscle LBP is a secreted, peripheral membrane protein with an unusual polyaspartate domain. Its laminin and membrane binding properties suggest that it may help mediate muscle cell interactions with the extracellular matrix. We propose the name "aspartactin" for this LBP

HR Del remnant anatomy using 2-D spectral data and 3-D photoionization shell models

The HR Del nova remnant was observed with the IFU-GMOS at Gemini North. The spatially resolved spectral data cube was used in the kinematic, morphological and abundance analysis of the ejecta. The line maps show a very clumpy shell with two main symmetric structures. The first one is the outer part of the shell seen in H-alpha, that forms two rings projected in the sky plane. These ring structures correspond to a closed hourglass shape, first proposed by Harman and O'Brien (2003). The equatorial emission enhancement is caused by the superimposed hourglass structures in the line of sight. The second structure seen only in the [OIII] and [NII] maps is located along the polar directions inside the hourglass structure. Abundances gradients between the polar caps and equatorial region were not found. However, the outer part of the shell seems to be less abundant in Oxygen and Nitrogen than the inner regions. Detailed 2.5D photoionization modeling of the 3D shell was performed using the mass distribution inferred from the observations and the presence of mass clumps. The resulting model grids are used to constrain the physical properties of the shell as well as the central ionizing source. A sequence of 3D clumpy models including a disk shaped ionization source is able to reproduce the ionization gradients between polar and equatorial regions of the shell. Differences between shell axial ratios in different lines can also be explained by aspherical illumination. A total shell mass of 9 x 10-4 Msun is derived from these models. We estimate that 50% to 70% of the shell mass is contained in neutral clumps with density contrast up to a factor of 30.Comment: 31 pages 12 figures 4 tables title correcte

Exploring the fate of cattle herds with inconclusive reactors to the tuberculin skin test

Bovine tuberculosis (TB) is an important animal health issue in many parts of the world. In England and Wales, the primary test to detect infected animals is the single intradermal comparative cervical tuberculin test, which compares immunological responses to bovine and avian tuberculins. Inconclusive test reactors (IRs) are animals that demonstrate a positive reaction to the bovine tuberculin only marginally greater than the avian reaction, so are not classified as reactors and immediately removed. In the absence of reactors in the herd, IRs are isolated, placed under movement restrictions and re-tested after 60 days. Other animals in these herds at the time of the IR result are not usually subject to movement restrictions. This could affect efforts to control TB if undetected infected cattle move out of those herds before the next TB test. To improve our understanding of the importance of IRs, this study aimed to assess whether median survival time and the hazard of a subsequent TB incident differs in herds with only IRs detected compared with negative-testing herds. Survival analysis and extended Cox regression were used, with herds entering the study on the date of the first whole herd test in 2012. An additional analysis was performed using an alternative entry date to try to remove the impact of IR retesting and is presented in the Supplementary Material. Survival analysis showed that the median survival time among IR only herds was half that observed for clear herds (2.1 years and 4.2 years respectively; p < 0.001). Extended Cox regression analysis showed that IR-only herds had 2.7 times the hazard of a subsequent incident compared with negative-testing herds in year one (hazard ratio: 2.69; 95% CI: 2.54, 2.84; p < 0.001), and that this difference in the hazard reduced by 63% per year. After 2.7 years the difference had disappeared. The supplementary analysis supported these findings showing that IR only herds still had a greater hazard of a subsequent incident after the IR re-test, but that the effect was reduced. This emphasizes the importance of careful decision making around the management of IR animals and indicates that re-testing alone may not be sufficient to reduce the risk posed by IR only herds in England and Wales

On the Complexity of Hilbert Refutations for Partition

Given a set of integers W, the Partition problem determines whether W can be divided into two disjoint subsets with equal sums. We model the Partition problem as a system of polynomial equations, and then investigate the complexity of a Hilbert's Nullstellensatz refutation, or certificate, that a given set of integers is not partitionable. We provide an explicit construction of a minimum-degree certificate, and then demonstrate that the Partition problem is equivalent to the determinant of a carefully constructed matrix called the partition matrix. In particular, we show that the determinant of the partition matrix is a polynomial that factors into an iteration over all possible partitions of W.Comment: Final versio

OH rotational lines as a diagnostic of the warm neutral gas in galaxies

We present Infrared Space Observatory (ISO) observations of several OH, CH and H2O rotational lines toward the bright infrared galaxies NGC253 and NGC1068. As found in the Galactic clouds in SgrB2 and Orion, the extragalactic far-IR OH lines change from absorption to emission depending on the physical conditions and distribution of gas and dust along the line of sight. As a result, most of the OH rotational lines that appear in absorption toward NGC253 are observed in emission toward NGC1068. We show that the far-IR spectrum of OH can be used as a powerful diagnostic to derive the physical conditions of extragalactic neutral gas. In particular, we find that a warm (Tk~150 K, n(H2)< 5 10^4 cm^-3) component of molecular gas with an OH abundance of 10^{-7} from the inner <15'' can qualitatively reproduce the OH lines toward NGC253. Similar temperatures but higher densities (5 10^5 cm^-3) are required to explain the OH emission in NGC1068.Comment: 5 pages, 4 figures, accepted in ApJ Part I (2004, October 6

The Role of Management Practices in Closing the Productivity Gap

There is no doubt that management practices are linked to the productivity and performance of a company. However, research findings are mixed. This paper provides a multi-disciplinary review of the current evidence of such a relationship and offers suggestions for further exploration. We provide an extensive review of the literature in terms of research findings from studies that have been trying to measure and understand the impact that individual management practices and clusters of management practices have on productivity at different levels of analysis. We focus our review on Operations Management (om) and Human Resource Management (hrm) practices as well as joint applications of these practices. In conclusion, we can say that taken as a whole, the research findings are equivocal. Some studies have found a positive relationship between the adoption of management practices and productivity, some negative and some no association whatsoever. We believe that the lack of universal consensus on the effect of the adoption of complementary management practices might be driven either by measurement issues or by the level of analysis. Consequently, there is a need for further research. In particular, for a multi-level approach from the lowest possible level of aggregation up to the firm-level of analysis in order to assess the impact of management practices upon the productivity of firms

Alkali-metal-mediated zincation (AMMZn) meets N-heterocyclic carbene (NHC) chemistry : Zn–H exchange reactions and structural authentication of a dinuclear Au(I) complex with a NHC anion

Merging two evolving areas in synthesis, namely cooperative bimetallics and N-heterocyclic carbenes (NHCs), this study reports the isolation of the first intermediates of alkali-metal-mediated zincation (AMMZn) of a free NHC and a Zn–NHC complex using sodium zincate [(TMEDA)NaZn(TMP)(tBu)2] (1) as a metallating reagent. The structural authentication of (THF)3Na[:C{[N(2,6-iPr2C6H3)]2CHCZn(tBu2)}] (2) and [Na(THF)6]+[tBu2Zn:C{[N(2,6-iPr2C6H3)]2CHCZn(tBu2)}]− (4), resulting from the reactions of 1 with unsaturated free NHC IPr (IPr = 1,3-bis(2,6-di-isopropylphenylimidazole-2-ylidene) and NHC complex ZntBu2IPr (3) respectively demonstrates that in both cases, this mixed-metal approach can easily facilitate the selective C4 zincation of the unsaturated backbone of the NHC ligand. Furthermore, the generation of anionic NHC fragments enables dual coordination through their normal (C2) and abnormal (C4) positions to the bimetallic system, stabilising the kinetic AMMZn intermediates which normally go undetected and provides new mechanistic insights in to how these mixed-metal reagents operate. In stark contrast to this bimetallic approach when NHC-complex 3 is reacted with a more conventional single-metal base such as tBuLi, the deprotonation of the coordinated carbene is inhibited, favouring instead, co-complexation to give NHC-stabilised [IPr·LiZntBu3] (5). Showing the potential of 2 to act as a transfer agent of its anionic NHC unit to transition metal complexes, this intermediate reacts with two molar equivalents of [ClAu(PPh3)] to afford the novel digold species [ClAu:C{[N(2,6-iPr2C6H3)]2CHCAu(PPh3)}] (6) resulting from an unprecedented double transmetallation reaction which involves the simultaneous exchange of both cationic (Na+) and neutral (ZntBu2) entities on the NHC framework

Characterization of an INVS Model IV Neutron Counter for High Precision (γ,n\gamma,n) Cross-Section Measurements

A neutron counter designed for assay of radioactive materials has been adapted for beam experiments at TUNL. The cylindrical geometry and 60% maximum efficiency make it well suited for ($\gamma,n$) cross-section measurements near the neutron emission threshold. A high precision characterization of the counter has been made using neutrons from several sources. Using a combination of measurements and simulations, the absolute detection efficiency of the neutron counter was determined to an accuracy of $\pm$ 3% in the neutron energy range between 0.1 and 1 MeV. It is shown that this efficiency characterization is generally valid for a wide range of targets.Comment: 22 pages, 13 figure

Putting the geology back into Earth models

New digital methods for data capture can now provide photorealistic, spatially precise, and geometrically accurate three-dimensional (3-D) models of rocks exposed at the Earth's surface [Xu et al., 2000; Pringle et al., 2001; Clegg et al., 2005]. These “virtual outcrops” have the potential to create a new form of laboratory-based teaching aids for geoscience students, to help address accessibility issues in fieldwork, and generally to improve public awareness of the spectacular nature of geologic exposures from remote locations worldwide. This article addresses how virtual outcrops can provide calibration, or a quantitative “reality check,” for a new generation of high-resolution predictive models for the Earth's subsurface