Curvature and torsion in growing actin networks

Intracellular pathogens such as Listeria monocytogenes and Rickettsia rickettsii move within a host cell by polymerizing a comet-tail of actin fibers that ultimately pushes the cell forward. This dense network of cross-linked actin polymers typically exhibits a striking curvature that causes bacteria to move in gently looping paths. Theoretically, tail curvature has been linked to details of motility by considering force and torque balances from a finite number of polymerizing filaments. Here we track beads coated with a prokaryotic activator of actin polymerization in three dimensions to directly quantify the curvature and torsion of bead motility paths. We find that bead paths are more likely to have low rather than high curvature at any given time. Furthermore, path curvature changes very slowly in time, with an autocorrelation decay time of 200 seconds. Paths with a small radius of curvature, therefore, remain so for an extended period resulting in loops when confined to two dimensions. When allowed to explore a 3D space, path loops are less evident. Finally, we quantify the torsion in the bead paths and show that beads do not exhibit a significant left- or right-handed bias to their motion in 3D. These results suggest that paths of actin-propelled objects may be attributed to slow changes in curvature rather than a fixed torque

The PSI-U1 snRNP interaction regulates male mating behavior in Drosophila

Alternative pre-mRNA splicing (AS) is a critical regulatory mechanism that operates extensively in the nervous system to produce diverse protein isoforms. Fruitless AS isoforms have been shown to influence male courtship behavior, but the underlying mechanisms are unknown. Using genome-wide approaches and quantitative behavioral assays, we show that the P-element somatic inhibitor (PSI) and its interaction with the U1 small nuclear ribonucleoprotein complex (snRNP) control male courtship behavior. PSI mutants lacking the U1 snRNP-interacting domain (PSIΔAB mutant) exhibit extended but futile mating attempts. The PSIΔAB mutant results in significant changes in the AS patterns of ∼1,200 genes in the Drosophila brain, many of which have been implicated in the regulation of male courtship behavior. PSI directly regulates the AS of at least one-third of these transcripts, suggesting that PSI-U1 snRNP interactions coordinate the behavioral network underlying courtship behavior. Importantly, one of these direct targets is fruitless, the master regulator of courtship. Thus, PSI imposes a specific mode of regulatory control within the neuronal circuit controlling courtship, even though it is broadly expressed in the fly nervous system. This study reinforces the importance of AS in the control of gene activity in neurons and integrated neuronal circuits, and provides a surprising link between a pleiotropic pre-mRNA splicing pathway and the precise control of successful male mating behavior

Load fluctuations drive actin network growth

The growth of actin filament networks is a fundamental biological process that drives a variety of cellular and intracellular motions. During motility, eukaryotic cells and intracellular pathogens are propelled by actin networks organized by nucleation-promoting factors, which trigger the formation of nascent filaments off the side of existing filaments in the network. A Brownian ratchet (BR) mechanism has been proposed to couple actin polymerization to cellular movements, whereby thermal motions are rectified by the addition of actin monomers at the end of growing filaments. Here, by following actin--propelled microspheres using three--dimensional laser tracking, we find that beads adhered to the growing network move via an object--fluctuating BR. Velocity varies with the amplitude of thermal fluctuation and inversely with viscosity as predicted for a BR. In addition, motion is saltatory with a broad distribution of step sizes that is correlated in time. These data point to a model in which thermal fluctuations of the microsphere or entire actin network, and not individual filaments, govern motility. This conclusion is supported by Monte Carlo simulations of an adhesion--based BR and suggests an important role for membrane tension in the control of actin--based cellular protrusions.Comment: To be published in PNA

High Power Cyclotrons for the Neutrino Experiments DAEδALUS and IsoDAR

DAEδALUS (Decay At rest Experiment for δcp At a Laboratory for Underground Science) has been proposed to measure the value of the CP violating phase delta through the oscillation of low energy muon anti-neutrinos to electron antineutrinos. With a single large detector, three accelerators at different distances enable the oscillation to be measured with sufficient accuracy. We have proposed the superconducting multi-megawatt DAEδALUS Supercinducting Ring Cyclotron (DSRC) as the means of producing the 800 MeV 12 mA protons required, through the acceleration of H2+, ions with highly efficient stripping extraction. The DSRC comprises twin ion sources and injector cyclotrons, followed by a booster. The injector cyclotron can also be used for a separate experiment, IsoDAR (Isotope Decay At Rest) in which low energy protons produce Lithium 8, and thus a very pure electron antineutrino source which can be used to measure, or rule out, short range oscillation to a sterile neutrino. We describe recent developments in the designs of the injector and the booster, and the prospects for the two experiments

Leptonic CP violation studies at MiniBooNE in the (3+2) sterile neutrino oscillation hypothesis

We investigate the extent to which leptonic CP-violation in (3+2) sterile neutrino models leads to different oscillation probabilities for $\bar{\nu}_{\mu}\to\bar{\nu}_e$ and $\nu_{\mu}\to\nu_e$ oscillations at MiniBooNE. We are using a combined analysis of short-baseline (SBL) oscillation results, including the LSND and null SBL results, to which we impose additional constraints from atmospheric oscillation data. We obtain the favored regions in MiniBooNE oscillation probability space for both (3+2) CP-conserving and (3+2) CP-violating models. We further investigate the allowed CP-violation phase values and the MiniBooNE reach for such a CP violation measurement. The analysis shows that the oscillation probabilities in MiniBooNE neutrino and antineutrino running modes can differ significantly, with the latter possibly being as much as three times larger than the first. In addition, we also show that all possible values of the single CP-violation phase measurable at short baselines in (3+2) models are allowed within 99% CL by existing data.Comment: Fixed a typo following PRD Erratum. 8 pages, 5 figure

Phenomenological Determination of the Beauty Meson Decay Parameter fBf_B and the CP-Violating Angle δ\delta

We fit the ${\cal CKM}$-matrix to all recent data with the following free parameters: three mixing angles, the CP-violating angle $\delta$ in the Maiani parametrisation, the top quark mass $m_t$, and the product f_B{\cal B}_{\B}^{1/2}, where $f_B$ is the $B$-meson decay parameter and {\cal B}_{\B} is the bag parameter. Our fits span a contiguous region in the (f_B{\cal B}_{\B}^{1/2},\ \cos\delta)--plane, limited by 0.117\lsim f_B{\cal B}_{\B}^{1/2}/{\rm GeV}\lsim 0.231 and --0.95 \lsim $\cos\delta$ \lsim 0.70. The parameters f_B{\cal B}_{\B}^{1/2} and $\cos\delta$ are strongly positively correlated.Comment: 9 pages + 1 figure available upon request, HU-TFT-94-3

Low energy neutrino scattering measurements at future Spallation Source facilities

In the future several Spallation Source facilities will be available worldwide. Spallation Sources produce large amount of neutrinos from decay-at-rest muons and thus can be well adapted to accommodate state-of-the-art neutrino experiments. In this paper low energy neutrino scattering experiments that can be performed at such facilities are reviewed. Estimation of expected event rates are given for several nuclei, electrons and protons at a detector located close to the source. A neutrino program at Spallation Sources comprises neutrino-nucleus cross section measurements relevant for neutrino and core-collapse supernova physics, electroweak tests and lepton-flavor violation searches.Comment: 12 pages, 4 figures, 5 table

First Measurement of Monoenergetic Muon Neutrino Charged Current Interactions

We report the first measurement of monoenergetic muon neutrino charged current interactions. MiniBooNE has isolated 236 MeV muon neutrino events originating from charged kaon decay at rest ($K^+ \rightarrow \mu^+ \nu_\mu$) at the NuMI beamline absorber. These signal $\nu_\mu$-carbon events are distinguished from primarily pion decay in flight $\nu_\mu$ and $\overline{\nu}_\mu$ backgrounds produced at the target station and decay pipe using their arrival time and reconstructed muon energy. The significance of the signal observation is at the 3.9$\sigma$ level. The muon kinetic energy, neutrino-nucleus energy transfer ($\omega=E_\nu-E_\mu$), and total cross section for these events is extracted. This result is the first known-energy, weak-interaction-only probe of the nucleus to yield a measurement of $\omega$ using neutrinos, a quantity thus far only accessible through electron scattering.Comment: 6 pages, 4 figure