37,801 research outputs found
Field and photon enhanced electron emission characteristics of cadmium sulphide field emitters
Field and photon enhanced electron emission characteristics of cadmium sulfide field emitter
Planar cell polarity genes Frizzled3a, Vangl2, and Scribble are required for spinal commissural axon guidance
Background A fundamental feature of early nervous system development is the guidance of axonal projections to their targets in order to assemble neural circuits that control behavior. Spinal commissural neurons are an attractive model to investigate the multiple guidance cues that control growth cone navigation both pre- and post-midline crossing, as well as along both the dorsal–ventral (D–V) and anterior–posterior (A–P) axes. Accumulating evidence suggests that guidance of spinal commissural axons along the A–P axis is dependent on components of the planar cell polarity (PCP) signaling pathway. In the zebrafish, the earliest born spinal commissural neuron to navigate the midline and turn rostrally is termed commissural primary ascending (CoPA). Unlike mammalian systems, CoPA axons cross the midline as a single axon and allow an analysis of the role of PCP components in anterior pathfinding in single pioneering axons. Results Here, we establish CoPA cells in the zebrafish spinal cord as a model system for investigating the molecular function of planar cell polarity signaling in axon guidance. Using mutant analysis, we show that the functions of Fzd3a and Vangl2 in the anterior turning of commissural axons are evolutionarily conserved in teleosts. We extend our findings to reveal a role for the PCP gene scribble in the anterior guidance of CoPA axons. Analysis of single CoPA axons reveals that these commissural axons become responsive to PCP-dependent anterior guidance cues even prior to midline crossing. When midline crossing is prevented by dcc gene knockdown, ipsilateral CoPA axons still extend axons anteriorly in response to A–P guidance cues. We show that this ipsilateral anterior pathfinding that occurs in the absence of midline crossing is dependent on PCP signaling. Conclusion Our results demonstrate that anterior guidance decisions by CoPA axons are dependent on the function of planar cell polarity genes both prior to and after midline crossing
Recommended from our members
Towards a Theory of Practice: Critical Transdisciplinary Multiliteracies
About the book: Education institutions and organizations throughout the world are currently being held accountable for achieving and maintaining historically unmatched standards of academic quality and performance. Accreditation bodies; policy makers; boards of trustees; and teacher, parent, and student groups all place educational institutions and organizations under unprecedented accountability pressures. The aim of this volume is to explore and better understand how these pressures are impacting a broad range of social and cultural issues and, subsequently, how these issues impact student motivation and learnin
The time resolved measurement of ultrashort THz-band electric fields without an ultrashort probe
The time-resolved detection of ultrashort pulsed THz-band electric field
temporal profiles without an ultrashort laser probe is demonstrated. A
non-linear interaction between a narrow-bandwidth optical probe and the THz
pulse transposes the THz spectral intensity and phase information to the
optical region, thereby generating an optical pulse whose temporal electric
field envelope replicates the temporal profile of the real THz electric field.
This optical envelope is characterised via an autocorrelation based FROG
measurement, hence revealing the THz temporal profile. The combination of a
narrow-bandwidth, long duration, optical probe and self-referenced FROG makes
the technique inherently immune to timing jitter between the optical probe and
THz pulse, and may find particular application where the THz field is not
initially generated via ultrashort laser methods, such as the measurement of
longitudinal electron bunch profiles in particle accelerators.Comment: 7 pages, 3 figures, submitted to AP
Aggregating Dependency Graphs into Voting Agendas in Multi-Issue Elections
Many collective decision making problems have a
combinatorial structure: the agents involved must
decide on multiple issues and their preferences over
one issue may depend on the choices adopted for
some of the others. Voting is an attractive method
for making collective decisions, but conducting a
multi-issue election is challenging. On the one hand,
requiring agents to vote by expressing their preferences
over all combinations of issues is computationally
infeasible; on the other, decomposing the
problem into several elections on smaller sets of
issues can lead to paradoxical outcomes. Any pragmatic
method for running a multi-issue election will
have to balance these two concerns. We identify
and analyse the problem of generating an agenda
for a given election, specifying which issues to vote
on together in local elections and in which order to
schedule those local elections
- …