Distinguishing wet from dry age-related macular degeneration using three-dimensional computer-automated threshold Amsler grid testing

Background/aims: With the increased efficacy of current therapy for wet age-related macular degeneration (AMD), better ways to detect wet AMD are needed. This study was designed to test the ability of three-dimensional contrast threshold Amsler grid (3D-CTAG) testing to distinguish wet AMD from dry AMD. Methods: Conventional paper Amsler grid and 3D-CTAG tests were performed in 90 eyes: 63 with AMD (34 dry, 29 wet) and 27 controls. Qualitative comparisons were based upon the three-dimensional shapes of central visual field (VF) defects. Quantitative analyses considered the number and volume of the three-dimensional defects. Results: 25/34 (74%) dry AMD and 6/29 (21%) wet AMD eyes had no distortions on paper Amsler grid. Of these, 5/25 (20%) dry and 6/6 (100%) wet (p=0.03) AMD eyes exhibited central VF defects with 3D-CTAG. Wet AMD displayed stepped defects in 16/28 (57%) eyes, compared with only 2/34 (6%) of dry AMD eyes (p=0.002). All three volumetric indices of VF defects were two- to four-fold greater in wet than dry AMD (p<0.006). 3D-CTAG had 83.9% positive and 90.6% negative predictive values for wet AMD. Conclusions: 3D-CTAG has a higher likelihood of detecting central VF defects than conventional Amsler grid, especially in wet AMD. Wet AMD can be distinguished from dry AMD by qualitative and quantitative 3D-CTAG criteria. Thus, 3D-CTAG may be useful in screening for wet AMD, quantitating disease severity, and providing a quantitative outcome measure of therapy

Coccidia (Apicomplexa: Eimeriidae) of Three-toed Box Turtles, Terrapene carolina triunguis (Reptilia: Testudines), from Arkansas and Oklahoma

We collected 50 three-toed box turtles (Terrapene carolina triunguis) from 9 counties of Arkansas and 4 counties of Oklahoma, and examined their feces for coccidial parasites. Nine of 24 (38%) turtles from Arkansas and 8 of 26 (31%) from Oklahoma were found to be passing oocysts of Eimeria ornata. This represents two new geographic distributional records for this coccidian. Measurements of individual isolates of E. ornate as well as morphological characteristics are provided with comparison to its original description and to another Terrapene coccidian, Eimeria carri. In addition, we noted an adelid pseudoparasite being passed by a single T. c. triunguis from Oklahoma that likely represents a parasite of arthropods

Vertebrate Natural History Notes from Arkansas, 2017

Because meaningful observations of natural history are not always part of larger studies, important pieces of information often are unreported. Small details, however, can fills gaps in understanding and also lead to interesting questions about ecological relationships or environmental change. We have compiled recent observations of foods, reproduction, record size, parasites, and distribution of 30 species of fishes, new records of distribution and parasites of 2 species of amphibians, and new records of distribution, parasites, reproduction and anomalies of 11 species of mammals

The role of upstream sequences in selecting the reading frame on tmRNA

<p>Abstract</p> <p>Background</p> <p>tmRNA acts first as a tRNA and then as an mRNA to rescue stalled ribosomes in eubacteria. Two unanswered questions about tmRNA function remain: how does tmRNA, lacking an anticodon, bypass the decoding machinery and enter the ribosome? Secondly, how does the ribosome choose the proper codon to resume translation on tmRNA? According to the -1 triplet hypothesis, the answer to both questions lies in the unique properties of the three nucleotides upstream of the first tmRNA codon. These nucleotides assume an A-form conformation that mimics the codon-anticodon interaction, leading to recognition by the decoding center and choice of the reading frame. The -1 triplet hypothesis is important because it is the most credible model in which direct binding and recognition by the ribosome sets the reading frame on tmRNA.</p> <p>Results</p> <p>Conformational analysis predicts that 18 triplets cannot form the correct structure to function as the -1 triplet of tmRNA. We tested the tmRNA activity of all possible -1 triplet mutants using a genetic assay in <it>Escherichia coli</it>. While many mutants displayed reduced activity, our findings do not match the predictions of this model. Additional mutagenesis identified sequences further upstream that are required for tmRNA function. An immunoblot assay for translation of the tmRNA tag revealed that certain mutations in U85, A86, and the -1 triplet sequence result in improper selection of the first codon and translation in the wrong frame (-1 or +1) <it>in vivo</it>.</p> <p>Conclusion</p> <p>Our findings disprove the -1 triplet hypothesis. The -1 triplet is not required for accommodation of tmRNA into the ribosome, although it plays a minor role in frame selection. Our results strongly disfavor direct ribosomal recognition of the upstream sequence, instead supporting a model in which the binding of a separate ligand to A86 is primarily responsible for frame selection.</p

Vertebrate Natural History Notes from Arkansas, 2016

Often, interesting and important observations of vertebrate distribution and natural history are not published because they are not part of a larger study. Knowledge of small details, however, not only fills gaps in understanding but also lead researchers to interesting questions about ecological relationships or environmental change. We have compiled recent observations of importance that can add immensely to the growth of knowledge of the biology of vertebrates in Arkansas

Racial and ethnic disparities in pelvic floor disorder awareness

Objectives: Pelvic floor disorders (PFD) affect the quality of life for many women and can be a significant cause of distress. With the United States’ aging population, PFD are becoming increasingly prevalent. Previous population based studies identified both differences in prevalence and baseline knowledge of PFD by race and ethnicity. These prior studies assessing PFD knowledge were limited to single geographical areas or institutions. We aim to conduct a multicenter study across the United States to better understand the baseline knowledge and perceptions of PFD and its variance across different races and ethnicities. Our goal is to provide data that will help deliver culturally competent information to patients. Materials and Methods: This was a multicenter cross-sectional study involving six sites. Upon initial presentation to a urogynecology clinic, patients were evaluated with the Prolapse and Incontinence Knowledge Questionnaire along with additional questions aimed to assess differences in PFD descriptive language, barriers to care, and attitude towards PFD. Surveys were distributed at participating institutions from October 2019 to February 2021. Statistical analyses were performed with Fisher\u27s exact test and Chi-square test, using p \u3c0.05 for statistical significance. Results: A total of 287 women completed the survey, with 27 excluded due to omission of race and or ethnicity responses. Respondents identified their ethnicity as Hispanic (21.8%) and race as White (70.5%), African American (AA) (19.2%), and Other Women of Color (OWOC) (10.3%). Overall, the mean percent correct for the urinary incontinence (UI) and pelvic organ prolapse (POP) sub-scores were 61.2 ± 28.2 and 60.5 ± 28.2, respectively. AA and OWOC had lower scores compared to White respondents in both UI (AA 60.7 ± 30.1; OWOC 46.8 ± 31.2; W 63.5 ± 26.5; p = 0.018) and POP (AA 55.5 ±30.1; OWOC 48.9 ± 31.0; W 64 ± 26.2; p = 0.011). Hispanic women scored lower in both UI (43.9 ± 29.8 vs 66.5 ± 25.3, p \u3c0.001) and POP (46.2 ± 28.4 vs 66.5 ± 25.3, p\u3c0.001) when compared to non-Hispanic respondents. AA and OWOC had decreased knowledge of POP language compared to White respondents (32.3 ± 30.8 % correct vs 35.4 ± 33.6 % correct vs 50.4 ± 32.9 % correct, p \u3c0.001). This was similar for Hispanic compared to non-Hispanic respondents (33.3 ± 31.2 % correct vs 40.1 ± 32.5 % correct, p\u3c0.001). Responses to UI and POP misconception questions were not different by race or ethnicity. Conclusion: This is the first multicenter, cross-sectional survey of a diverse patient population assessing knowledge and perception of PFD. We observed differences between baseline knowledge and language proficiency of PFD amongst AA, White, and OWOC, as well as between Hispanic and non-Hispanic women. This suggests the presence of racial disparities in women pursuing urogynecologic care and highlights the importance of delivering culturally sensitive information to educate women of diverse backgrounds