Reduction of in-shell Brazil nut (Bertholletia excelsa H.B.K.) aflatoxin contamination by ozone gas application during storage

The susceptibility of the in-shell Brazil nut mycoflora and aflatoxins (AFLs) contamination to ozone (O3) gas during storage is reported. In-shell Brazil nuts obtained from retail market were submitted to O3 gas atmosphere at different concentrations immediately before to be stored. Samples were collected just after the gas exposure and every 30 days during the storage period to carry on mycological tests and AFLs analysis. A sensorial evaluation by descriptive quality analysis was carried out to check treated nuts sensory attributes according to consumer acceptance after gas exposure. The O3 treatment applied within 5 h at 31 mg/L was able to successfully inhibit the viability of fungi of the nut-contaminating microflora and so the toxigenic Aspergillus species from the day of application. AFLs were totally degraded in all samples whatever O3 concentration applied. No significant changes on sensory attributes were observed that could affect nut acceptability  after the O3 treatments and storage conditions applied in the present experiment. This procedure is tentatively applied at an Amazon State nut factory for checking its potential in mycotoxin risk contamination of in-shell Brazil nuts safeguarding under the Amazon region environment. Keywords: In-shell Brazil nut, Ozone, Mycoflora, Aflatoxin, Storage, Sensory evaluation

Effect of oxygen reducing atmospheres on the quality and safety of stored shelled Brazil nut packs

High moisture content, relative humidity, temperature and environment rich in oxygen (O2) are the main factors for tree nuts to get infected by fungi and so aflatoxins (AFLs) contaminated. During storage and commercialization dry Brazil nuts packs need to maintain their safety and quality. Modified atmospheres in storage (macro-environment) and packaging (micro-environment) have been used to prolong food shelf life by reducing O2 concentration with inhibitory gases or, more recently, by adding O2 absorber pads. This work reports the application of O2 atmosphere reducing methods on stored shelled Brazil nut packs aiming fungi and AFL degradation as well as hygienic conditions improvements. The methods applied were: (a) ozone - O3, (b) carbon dioxide - CO2 and (c) O2 absorber pads with and without vacuum. Nuts were submitted to microbiological tests (fungi, aflatoxigenic strains, yeast and bacteria), moisture content and AFLs analysis. From all O2 reducing atmosphere evaluated, the best performance was obtained with O3. A reduction on fungi growth (1.8 x 104 cfu.g-1 to 2.6 x 10 cfu.g-1) and yeast destruction after the first month of storage were registered. Also O3 was the only nut treatment that was able to degrade AFLs. None of the spiked (AFLs: 15 ppb) nut samples O3 treated had AFLs detected up to the LOQ of the method (0.36 μg.kg-1 for AFB1+AFB2+AFG1+AFG2) i.e., much lower than the allowed by the European Union regulation (MRL: 4 and 2 ppb for total and AFB1, respectively), thus producing safer nuts. All other treatments stabilized and/or inhibited microorganisms growth. Add CO2 and O2 pads played an important role on nut quality. Further study will be carried out in order to adjust O3 concentration and application conditions for longer period of storage

Diversidade genética ente progênies de macaúba na fase de muda.

A macaúba (Acrocomia aculeata) é uma palmeira arborescente, espinhosa, de até 20 m de altura. Conhecer a diversidade genética desta espécie é importante para o inicio do melhoramento. Assim, o objetivo deste trabalho foi determinar a divergência genética e agrupar 15 progênies de macaúba, baseado nas características da muda através de análise multivariada. Quinze matrizes de macaúba coletadas foram avaliadas quanto a duas características da planta na fase de muda. O experimento foi conduzido em blocos ao acaso, com cinco repetições e quinze genótipos. As características avaliadas das mudas com 12 meses de idade foram: Altura da Muda (cm) e Número de Folhas por muda. De acordo com os resultados obtidos para as características avaliadas observou-se a presença de variabilidade entre as 15 progênies. A existência de variabilidade genética entre as progênies permitiu separá-los pelo método de Tocher em oito grupos. As formações dos grupos variaram de uma a três progênies. O método utilizado agrupou as progênies com tendência da origem geográfica. Esta separação em grupos distintos é importante para o melhoramento genético da espécie

Protocolo de micropropagação para as espécies Piper hispidinervum e P. aduncum.

Este trabalho apresenta o protocolo de cultivo in vitro para as espécies Piper hispidinervum e P. aduncum, utilizando material oriundo de mudas obtidas por meio da germinação in vitro de sementes.bitstream/item/72680/1/24590.pd

Catálogo Taxonômico da Fauna do Brasil: setting the baseline knowledge on the animal diversity in Brazil

The limited temporal completeness and taxonomic accuracy of species lists, made available in a traditional manner in scientific publications, has always represented a problem. These lists are invariably limited to a few taxonomic groups and do not represent up-to-date knowledge of all species and classifications. In this context, the Brazilian megadiverse fauna is no exception, and the Catálogo Taxonômico da Fauna do Brasil (CTFB) (http://fauna.jbrj.gov.br/), made public in 2015, represents a database on biodiversity anchored on a list of valid and expertly recognized scientific names of animals in Brazil. The CTFB is updated in near real time by a team of more than 800 specialists. By January 1, 2024, the CTFB compiled 133,691 nominal species, with 125,138 that were considered valid. Most of the valid species were arthropods (82.3%, with more than 102,000 species) and chordates (7.69%, with over 11,000 species). These taxa were followed by a cluster composed of Mollusca (3,567 species), Platyhelminthes (2,292 species), Annelida (1,833 species), and Nematoda (1,447 species). All remaining groups had less than 1,000 species reported in Brazil, with Cnidaria (831 species), Porifera (628 species), Rotifera (606 species), and Bryozoa (520 species) representing those with more than 500 species. Analysis of the CTFB database can facilitate and direct efforts towards the discovery of new species in Brazil, but it is also fundamental in providing the best available list of valid nominal species to users, including those in science, health, conservation efforts, and any initiative involving animals. The importance of the CTFB is evidenced by the elevated number of citations in the scientific literature in diverse areas of biology, law, anthropology, education, forensic science, and veterinary science, among others

Dewatering of septic tank sludge in alternative sludge drying bed

In dewatering of septic tank sludge, the sludge drying bed is one of the main techniques used, but it demands a great deal of area and time for the removal of sludge cake. Modification of this system using permeable pavements and polymer can minimize such problems, facilitating the management of sludge when decentralized sanitation is used. Therefore, the objective of this study was to compare the dewatering of septic tank sludge using conventional sludge drying bed (CSDB) and a sludge drying bed with permeable pavement (SDBPP). At the same time of dewatering, the volume drained by the SDBPP was 37.4 ± 4.6% higher than that obtained in the CSDB. Therefore, a lower drying bed could be used. It was found that the use of synthetic polymer allowed dewatering to occur in less time, but did not interfere in the solids content of the sludge cake. The reuse of the pavement was proven possible, but required large volumes of water and mechanical equipment84792798CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP311275/2015-02017/07490-